(Circular) (Six-base) MAPSORT of: pFN21AB7737.seq Check: 9001 from: 1 to: 6013 pFN21AB7737 6013 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 3356 1937 Size: 583 836 4594 Fragments arranged by size: 4594 836 583 Acc65I G'GTAC_C Cuts at: 3195 3217 3334 3195 Size: 22 117 5874 Fragments arranged by size: 5874 117 22 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 4076 5460 5728 1632 Size: 1172 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 1172 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 3022 1166 Size: 1856 4157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 2390 2835 3756 5328 2390 Size: 445 921 1572 3075 Fragments arranged by size: 3075 1572 921 445 ApaI G_GGCC'C Cuts at: 2061 2882 3082 3251 2061 Size: 821 200 169 4823 Fragments arranged by size: 4823 821 200 169 ApaLI G'TGCA_C Cuts at: 2550 4070 5226 2550 Size: 1520 1156 3337 Fragments arranged by size: 3337 1520 1156 ApoI r'AATT_y Cuts at: 1493 3322 3548 3700 5809 1493 Size: 1829 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2269 2402 2729 2993 3338 1870 Size: 73 326 133 327 264 345 4545 Fragments arranged by size: 4545 345 327 326 264 133 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3016 3195 3217 3334 4789 Size: 325 217 627 1229 179 22 117 1455 Cuts at: 4789 618 Size: 1842 Fragments arranged by size: 1842 1455 1229 627 325 217 179 117 22 BanII G_rGCy'C Cuts at: 1391 2061 2452 2484 2502 2882 3082 3107 3191 Size: 670 391 32 18 380 200 25 84 Cuts at: 3191 3251 3299 3332 1391 Size: 60 48 33 4072 Fragments arranged by size: 4072 670 391 380 200 84 60 48 33 32 25 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5042 BbvCI CC'TCA_GC Cuts at: 3042 3042 Size: 6013 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2522 2556 4204 4238 1903 Size: 34 585 34 1648 34 3678 Fragments arranged by size: 3678 1648 585 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 2337 1548 Size: 789 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2900 4624 136 Size: 107 122 71 631 605 1228 1724 1525 Fragments arranged by size: 1724 1525 1228 631 605 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3139 3430 3139 Size: 291 5722 Bme1580I G_kGCm'C Cuts at: 1790 2061 2228 2554 2882 3082 3251 4074 5230 Size: 271 167 326 328 200 169 823 1156 Cuts at: 5230 1790 Size: 2573 Fragments arranged by size: 2573 1156 823 328 326 271 200 169 167 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3030 3078 Size: 416 53 83 399 24 1293 320 48 Cuts at: 3078 3744 4702 442 Size: 666 958 1753 Fragments arranged by size: 1753 1293 958 666 416 399 320 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2335 2335 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2232 2309 3108 3185 4673 1239 Size: 826 167 77 799 77 1488 2579 Fragments arranged by size: 2579 1488 826 799 167 77 77 Bpu10I CC'TnA_GC Cuts at: 2282 3042 2282 Size: 760 5253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2726 4676 882 Size: 617 1227 1950 2219 Fragments arranged by size: 2219 1950 1227 617 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2853 3820 4202 4837 Size: 53 83 186 936 1320 967 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1451 1320 967 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2437 4446 4844 5118 5265 5676 2437 Size: 2009 398 274 147 411 2774 Fragments arranged by size: 2774 2009 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3222 3252 1178 Size: 30 165 30 1819 30 3939 Fragments arranged by size: 3939 1819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6013 BseYI C'CCAG_C Cuts at: 2351 2475 2483 2682 2986 3107 3199 5216 5712 Size: 124 8 199 304 121 92 2017 496 Cuts at: 5712 2351 Size: 2652 Fragments arranged by size: 2652 2017 496 304 199 124 121 92 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 3041 1163 Size: 573 882 423 4135 Fragments arranged by size: 4135 882 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2554 4074 4159 5230 2554 Size: 1520 85 1071 3337 Fragments arranged by size: 3337 1520 1071 85 BsmI GAATG_Cn' Cuts at: 3524 3617 3524 Size: 93 5920 BsmBI CGTCTCn'nnnn_ Cuts at: 2844 2844 Size: 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2228 2452 2484 2502 2554 2882 Size: 399 271 167 224 32 18 52 328 Cuts at: 2882 3082 3107 3191 3251 3299 3332 4074 4159 Size: 200 25 84 60 48 33 742 85 Cuts at: 4159 5230 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 271 224 200 167 85 84 60 52 48 33 32 25 18 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3368 844 Size: 2524 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2913 3900 1394 Size: 429 1090 987 3507 Fragments arranged by size: 3507 1090 987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2233 2469 3211 4657 4844 1700 Size: 110 232 191 236 742 1446 187 2869 Fragments arranged by size: 2869 1446 742 236 232 191 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 2016 4073 5085 2016 Size: 2057 1012 2944 Fragments arranged by size: 2944 2057 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2641 794 Size: 1847 4166 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2971 3343 3710 4095 4112 5564 5650 5662 Size: 999 372 367 385 17 1452 86 12 Cuts at: 5662 6008 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 999 385 372 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2220 2617 3073 513 Size: 552 1155 397 456 3453 Fragments arranged by size: 3453 1155 552 456 397 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3529 4323 4343 3529 Size: 794 20 5199 Fragments arranged by size: 5199 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2004 2039 529 Size: 35 1440 35 4503 Fragments arranged by size: 4503 1440 35 35 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DraIII CAC_nnn'GTG Cuts at: 2927 2927 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2620 3460 Size: 54 1425 50 275 18 354 436 840 Cuts at: 3460 4349 5978 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 840 436 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2858 3943 1353 Size: 1505 1085 3423 Fragments arranged by size: 3423 1505 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2232 2309 2804 3108 3185 4076 Size: 393 433 167 77 495 304 77 891 Cuts at: 4076 4673 5460 5728 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 495 433 393 304 268 167 77 77 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 6013 EcoO109I rG'GnC_Cy Cuts at: 2057 2254 2878 2983 3078 3079 3247 3248 2057 Size: 197 624 105 95 1 168 1 4822 Fragments arranged by size: 4822 624 197 168 105 95 1 1 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 2476 3139 5160 1678 Size: 798 663 2021 2531 Fragments arranged by size: 2531 2021 798 663 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2370 2402 3281 3313 4654 Size: 32 135 32 798 32 879 32 1341 Cuts at: 4654 4686 4728 4760 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 879 798 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3199 3221 3338 3199 Size: 22 117 5874 Fragments arranged by size: 5874 117 22 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5127 5311 1305 Size: 3822 184 2007 Fragments arranged by size: 3822 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 3022 3971 4330 4489 518 Size: 648 1581 275 949 359 159 2042 Fragments arranged by size: 2042 1581 949 648 359 275 159 MspA1I CmG'CkG Cuts at: 1606 2460 2487 3008 3203 3429 4106 5254 5499 Size: 854 27 521 195 226 677 1148 245 Cuts at: 5499 1606 Size: 2120 Fragments arranged by size: 2120 1148 854 677 521 245 226 195 27 NaeI GCC'GGC Cuts at: 2044 2235 2471 2044 Size: 191 236 5586 Fragments arranged by size: 5586 236 191 NcoI C'CATG_G Cuts at: 513 1065 2220 3073 513 Size: 552 1155 853 3453 Fragments arranged by size: 3453 1155 853 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2042 2233 2469 2042 Size: 191 236 5586 Fragments arranged by size: 5586 236 191 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2365 3371 4916 1132 Size: 97 1136 1006 1545 2229 Fragments arranged by size: 2229 1545 1136 1006 97 PasI CC'CwG_GG Cuts at: 1348 2035 2947 1348 Size: 687 912 4414 Fragments arranged by size: 4414 912 687 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PfoI T'CCnGG_A Cuts at: 2156 2817 3114 2156 Size: 661 297 5055 Fragments arranged by size: 5055 661 297 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 2057 2878 3078 3247 2057 Size: 821 200 169 4823 Fragments arranged by size: 4823 821 200 169 PstI C_TGCA'G Cuts at: 830 2693 3365 830 Size: 1863 672 3478 Fragments arranged by size: 3478 1863 672 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SexAI A'CCwGG_T Cuts at: 2923 2923 Size: 6013 SfcI C'TryA_G Cuts at: 826 1045 2689 3361 4496 5177 5368 826 Size: 219 1644 672 1135 681 191 1471 Fragments arranged by size: 1644 1471 1135 681 672 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 3211 1700 Size: 1511 4502 SmaI CCC'GGG Cuts at: 1872 2271 2995 3340 1872 Size: 399 724 345 4545 Fragments arranged by size: 4545 724 399 345 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5018 5280 820 Size: 197 926 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 2903 3047 1517 Size: 1386 144 4483 Fragments arranged by size: 4483 1386 144 StyI C'CwwG_G Cuts at: 513 1065 2220 3073 3241 3452 513 Size: 552 1155 853 168 211 3074 Fragments arranged by size: 3074 1155 853 552 211 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2208 2939 4040 4057 4210 4395 4840 Size: 1255 628 731 1101 17 153 185 445 Cuts at: 4840 325 Size: 1498 Fragments arranged by size: 1498 1255 1101 731 628 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4259 5971 96 Size: 274 80 33 51 1443 2282 1712 138 Fragments arranged by size: 2282 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3222 3254 5973 6005 3222 Size: 32 2719 32 3230 Fragments arranged by size: 3230 2719 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2516 1316 Size: 1200 4813 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 2269 2993 3338 1870 Size: 399 724 345 4545 Fragments arranged by size: 4545 724 399 345 XmnI GAAnn'nnTTC Cuts at: 2312 4142 2312 Size: 1830 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI