(Circular) (Six-base) MAPSORT of: pFN21AB7321.seq Check: 9715 from: 1 to: 8302 pFN21AB7321 8302 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4435 4435 Size: 8302 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6112 278 Size: 53 83 186 936 4576 2468 Fragments arranged by size: 4576 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 4951 5645 1937 Size: 741 2273 694 4594 Fragments arranged by size: 4594 2273 741 694 Acc65I G'GTAC_C Cuts at: 2491 5623 2491 Size: 3132 5170 AclI AA'CG_TT Cuts at: 1563 6429 6802 1563 Size: 4866 373 3063 Fragments arranged by size: 4866 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 3134 4571 6365 7749 8017 1632 Size: 824 678 1437 1794 1384 268 1917 Fragments arranged by size: 1917 1794 1437 1384 824 678 268 AfeI AGC'GCT Cuts at: 5389 5389 Size: 8302 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8105 AflIII A'CryG_T Cuts at: 1203 2413 2891 4600 4788 7201 8178 1203 Size: 1210 478 1709 188 2413 977 1327 Fragments arranged by size: 2413 1709 1327 1210 977 478 188 AgeI A'CCGG_T Cuts at: 7133 7133 Size: 8302 AhdI GACnn_n'nnGTC Cuts at: 7031 7031 Size: 8302 AleI CACnn'nnGTG Cuts at: 1166 3478 1166 Size: 2312 5990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8302 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5099 5131 5099 Size: 32 8270 AlwNI CAG_nnn'CTG Cuts at: 6045 7617 6045 Size: 1572 6730 ApaLI G'TGCA_C Cuts at: 6359 7515 6359 Size: 1156 7146 ApoI r'AATT_y Cuts at: 1493 2119 2554 2618 2870 2883 3120 3404 3593 Size: 626 435 64 252 13 237 284 189 Cuts at: 3593 3926 4016 4123 4876 5611 5837 5989 8098 Size: 333 90 107 753 735 226 152 2109 Cuts at: 8098 1493 Size: 1697 Fragments arranged by size: 2109 1697 753 735 626 435 333 284 252 237 226 189 152 107 90 64 13 AseI AT'TA_AT Cuts at: 160 1032 6856 160 Size: 872 5824 1606 Fragments arranged by size: 5824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8302 AvaI C'yCGr_G Cuts at: 1870 1943 5627 1870 Size: 73 3684 4545 Fragments arranged by size: 4545 3684 73 BamHI G'GATC_C Cuts at: 3722 5632 5999 3722 Size: 1910 367 6025 Fragments arranged by size: 6025 1910 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2491 5623 7078 618 Size: 325 217 627 704 3132 1455 1842 Fragments arranged by size: 3132 1842 1455 704 627 325 217 BanII G_rGCy'C Cuts at: 1391 2028 5223 5621 1391 Size: 637 3195 398 4072 Fragments arranged by size: 4072 3195 637 398 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 2917 4262 928 Size: 971 572 446 1345 4968 Fragments arranged by size: 4968 1345 971 572 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6493 6527 1903 Size: 34 50 34 4472 34 3678 Fragments arranged by size: 4472 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5143 6194 7404 5143 Size: 1051 1210 6041 Fragments arranged by size: 6041 1210 1051 BclI T'GATC_A Cuts at: 1548 1548 Size: 8302 BfrBI ATG'CAT Cuts at: 2791 5374 2791 Size: 2583 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6913 136 Size: 107 122 71 631 605 5241 1525 Fragments arranged by size: 5241 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8297 8297 Size: 8302 BlpI GC'TnA_GC Cuts at: 5719 5719 Size: 8302 Bme1580I G_kGCm'C Cuts at: 1790 6363 7519 1790 Size: 4573 1156 2573 Fragments arranged by size: 4573 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2490 5302 6033 Size: 416 53 83 399 24 1073 2812 731 Cuts at: 6033 6991 442 Size: 958 1753 Fragments arranged by size: 2812 1753 1073 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5244 1056 Size: 4188 4114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2544 2544 Size: 8302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4011 4918 5199 5539 6962 1239 Size: 2772 907 281 340 1423 2579 Fragments arranged by size: 2772 2579 1423 907 340 281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5109 6427 7292 7590 5109 Size: 1318 865 298 5821 Fragments arranged by size: 5821 1318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6965 882 Size: 617 5466 2219 Fragments arranged by size: 5466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3685 6093 493 Size: 711 2481 2408 2702 Fragments arranged by size: 2702 2481 2408 711 BsaBI GATnn'nnATC Cuts at: 2444 3846 8296 2444 Size: 1402 4450 2450 Fragments arranged by size: 4450 2450 1402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6109 6491 7126 275 Size: 53 83 186 936 4576 382 635 1451 Fragments arranged by size: 4576 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6735 7133 7407 7554 7965 6735 Size: 398 274 147 411 7072 Fragments arranged by size: 7072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 2891 2921 3822 Size: 30 165 30 1257 30 201 30 901 Cuts at: 3822 3852 1178 Size: 30 5628 Fragments arranged by size: 5628 1257 901 201 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3209 5147 5150 1186 Size: 2023 1938 3 4338 Fragments arranged by size: 4338 2023 1938 3 BseYI C'CCAG_C Cuts at: 3057 5265 7505 8001 3057 Size: 2208 2240 496 3358 Fragments arranged by size: 3358 2240 2208 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3728 4410 1163 Size: 573 1992 682 5055 Fragments arranged by size: 5055 1992 682 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5752 6513 7541 1540 Size: 275 186 3751 761 1028 2301 Fragments arranged by size: 3751 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2530 4139 5466 6363 6448 7519 2530 Size: 1609 1327 897 85 1071 3313 Fragments arranged by size: 3313 1609 1327 1071 897 85 BsmI GAATG_Cn' Cuts at: 5813 5906 5813 Size: 93 8209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2530 4139 5223 5466 5621 6363 Size: 399 238 502 1609 1084 243 155 742 Cuts at: 6363 6448 7519 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1609 1084 1071 742 502 399 243 238 155 85 BspEI T'CCGG_A Cuts at: 7965 7965 Size: 8302 BspHI T'CATG_A Cuts at: 2683 2857 5158 6191 7921 2683 Size: 174 2301 1033 1730 3064 Fragments arranged by size: 3064 2301 1730 1033 174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4435 5562 5657 844 Size: 3591 1127 95 3489 Fragments arranged by size: 3591 3489 1127 95 BsrBI CCG'CTC Cuts at: 1394 1823 6189 1394 Size: 429 4366 3507 Fragments arranged by size: 4366 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2828 3864 4278 4733 4846 6797 Size: 1183 1395 185 1036 414 455 113 1951 Cuts at: 6797 6971 65 Size: 174 1396 Fragments arranged by size: 1951 1396 1395 1183 1036 455 414 185 174 113 BsrFI r'CCGG_y Cuts at: 1700 1810 6946 7133 1700 Size: 110 5136 187 2869 Fragments arranged by size: 5136 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2603 96 Size: 2507 5795 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8302 BssSI C'ACGA_G Cuts at: 6362 7374 6362 Size: 1012 7290 BstAPI GCAn_nnn'nTGC Cuts at: 794 2348 2642 3863 4118 794 Size: 1554 294 1221 255 4978 Fragments arranged by size: 4978 1554 1221 294 255 BstBI TT'CG_AA Cuts at: 7106 7106 Size: 8302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8302 BstYI r'GATC_y Cuts at: 1972 2779 3722 3847 5632 5999 6384 6401 7853 Size: 807 943 125 1785 367 385 17 1452 Cuts at: 7853 7939 7951 8297 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1785 1452 943 807 385 367 346 125 86 17 12 BstZ17I GTA'TAC Cuts at: 2679 2679 Size: 8302 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7750 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7563 Fragments arranged by size: 7563 683 56 BtsI GCAGTG_nn' Cuts at: 3572 3737 4076 5818 6612 6632 3572 Size: 165 339 1742 794 20 5242 Fragments arranged by size: 5242 1742 794 339 165 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8267 DraI TTT'AAA Cuts at: 4627 5190 5606 4627 Size: 563 416 7323 Fragments arranged by size: 7323 563 416 DraIII CAC_nnn'GTG Cuts at: 3370 4654 3370 Size: 1284 7018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7309 809 Size: 507 5993 1802 Fragments arranged by size: 5993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2751 4410 4645 Size: 54 1425 50 275 18 921 1659 235 Cuts at: 4645 5234 5749 6638 8267 8 Size: 589 515 889 1629 43 Fragments arranged by size: 1659 1629 1425 921 889 589 515 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5749 1537 Size: 275 3937 4090 Fragments arranged by size: 4090 3937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4175 6232 1353 Size: 2822 2057 3423 Fragments arranged by size: 3423 2822 2057 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6889 7263 7409 387 Size: 1064 138 5300 374 146 1280 Fragments arranged by size: 5300 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 3134 4011 4571 4918 5199 5539 Size: 393 824 678 877 560 347 281 340 Cuts at: 5539 6365 6962 7749 8017 1239 Size: 826 597 787 268 1524 Fragments arranged by size: 1524 877 826 824 787 678 597 560 393 347 340 281 268 EcoNI CCTnn'n_nnAGG Cuts at: 2364 4768 2364 Size: 2404 5898 EcoO109I rG'GnC_Cy Cuts at: 2431 2431 Size: 8302 EcoRI G'AATT_C Cuts at: 4876 5611 5989 4876 Size: 735 378 7189 Fragments arranged by size: 7189 735 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2495 2903 2941 3383 4186 2495 Size: 408 38 442 803 6611 Fragments arranged by size: 6611 803 442 408 38 FspI TGC'GCA Cuts at: 6808 6808 Size: 8302 HaeII r_GCGC'y Cuts at: 1678 5391 7449 1678 Size: 3713 2058 2531 Fragments arranged by size: 3713 2531 2058 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 2576 2608 3647 Size: 32 135 32 645 32 327 32 1039 Cuts at: 3647 3679 5359 5391 6943 6975 7017 7049 1373 Size: 32 1680 32 1552 32 42 32 2626 Fragments arranged by size: 2626 1680 1552 1039 645 327 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4794 5211 5646 5892 669 Size: 988 281 2856 417 435 246 3079 Fragments arranged by size: 3079 2856 988 435 417 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8302 HpaI GTT'AAC Cuts at: 5892 5892 Size: 8302 KpnI G_GTAC'C Cuts at: 2495 5627 2495 Size: 3132 5170 MfeI C'AATT_G Cuts at: 5901 5901 Size: 8302 MluI A'CGCG_T Cuts at: 8178 8178 Size: 8302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5077 7416 7600 1305 Size: 3772 2339 184 2007 Fragments arranged by size: 3772 2339 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2753 4412 4647 10 Size: 54 1425 343 921 1659 235 3665 Fragments arranged by size: 3665 1659 1425 921 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 2418 3478 4178 5457 5514 6260 6619 Size: 648 1252 1060 700 1279 57 746 359 Cuts at: 6619 6778 518 Size: 159 2042 Fragments arranged by size: 2042 1279 1252 1060 746 700 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2011 2339 5381 5718 6395 7543 7788 1606 Size: 405 328 3042 337 677 1148 245 2120 Fragments arranged by size: 3042 2120 1148 677 405 337 328 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7750 NdeI CA'TA_TG Cuts at: 387 3217 3477 387 Size: 2830 260 5212 Fragments arranged by size: 5212 2830 260 NheI G'CTAG_C Cuts at: 1052 5240 1052 Size: 4188 4114 NotI GC'GGCC_GC Cuts at: 5749 5749 Size: 8302 NsiI A_TGCA'T Cuts at: 2793 5376 2793 Size: 2583 5719 NspI r_CATG'y Cuts at: 1132 1229 2791 2895 4604 4792 5197 5660 7205 Size: 97 1562 104 1709 188 405 463 1545 Cuts at: 7205 1132 Size: 2229 Fragments arranged by size: 2229 1709 1562 1545 463 405 188 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8302 PciI A'CATG_T Cuts at: 2891 4600 4788 7201 2891 Size: 1709 188 2413 3992 Fragments arranged by size: 3992 2413 1709 188 PflMI CCAn_nnn'nTGG Cuts at: 1108 3052 4654 4979 1108 Size: 1944 1602 325 4431 Fragments arranged by size: 4431 1944 1602 325 PfoI T'CCnGG_A Cuts at: 3667 3667 Size: 8302 PmeI GTTT'AAAC Cuts at: 5606 5606 Size: 8302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6371 6403 7884 7916 6371 Size: 32 1481 32 6757 Fragments arranged by size: 6757 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2431 Size: 8302 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8302 PsiI TTA'TAA Cuts at: 2262 5872 2262 Size: 3610 4692 PstI C_TGCA'G Cuts at: 830 2344 4432 5654 830 Size: 1514 2088 1222 3478 Fragments arranged by size: 3478 2088 1514 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8302 PvuII CAG'CTG Cuts at: 2339 2339 Size: 8302 SalI G'TCGA_C Cuts at: 1936 5644 1936 Size: 3708 4594 SbfI CC_TGCA'GG Cuts at: 5654 5654 Size: 8302 ScaI AGT'ACT Cuts at: 3448 6550 8262 3448 Size: 3102 1712 3488 Fragments arranged by size: 3488 3102 1712 SfcI C'TryA_G Cuts at: 826 1045 2340 2727 4078 4250 4428 5418 5650 Size: 219 1295 387 1351 172 178 990 232 Cuts at: 5650 6785 7466 7657 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1351 1295 1135 990 681 387 232 219 191 178 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8302 SmaI CCC'GGG Cuts at: 1872 5629 1872 Size: 3757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5124 6406 7307 7569 820 Size: 197 926 3181 1282 901 262 1553 Fragments arranged by size: 3181 1553 1282 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6093 493 Size: 5600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8302 SphI G_CATG'C Cuts at: 1132 5660 1132 Size: 4528 3774 SspI AAT'ATT Cuts at: 5 52 6226 5 Size: 47 6174 2081 Fragments arranged by size: 6174 2081 47 StuI AGG'CCT Cuts at: 1517 5171 1517 Size: 3654 4648 StyI C'CwwG_G Cuts at: 513 1065 2072 2274 2748 3051 5741 513 Size: 552 1007 202 474 303 2690 3074 Fragments arranged by size: 3074 2690 1007 552 474 303 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3097 5100 5308 6329 6346 6499 6684 Size: 1255 1517 2003 208 1021 17 153 185 Cuts at: 6684 7129 325 Size: 445 1498 Fragments arranged by size: 2003 1517 1498 1255 1021 445 208 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2603 3446 4344 Size: 274 80 33 51 1443 626 843 898 Cuts at: 4344 4926 6548 8260 96 Size: 582 1622 1712 138 Fragments arranged by size: 1712 1622 1443 898 843 626 582 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8262 8294 8262 Size: 32 8270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8302 XbaI T'CTAG_A Cuts at: 3083 5638 3083 Size: 2555 5747 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2403 3065 4006 2403 Size: 662 941 6699 Fragments arranged by size: 6699 941 662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8302 XmaI C'CCGG_G Cuts at: 1870 5627 1870 Size: 3757 4545 XmnI GAAnn'nnTTC Cuts at: 2702 2883 4527 6431 2702 Size: 181 1644 1904 4573 Fragments arranged by size: 4573 1904 1644 181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6110 276 Size: 53 83 186 936 4576 2468 Fragments arranged by size: 4576 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI