(Circular) (Six-base) MAPSORT of: pFN21AA0162.seq Check: 5206 from: 1 to: 9883 pFN21AA0162 9883 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3805 4282 6610 3805 Size: 477 2328 7078 Fragments arranged by size: 7078 2328 477 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7693 278 Size: 53 83 186 936 6157 2468 Fragments arranged by size: 6157 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3393 7226 1937 Size: 1456 3833 4594 Fragments arranged by size: 4594 3833 1456 Acc65I G'GTAC_C Cuts at: 2071 6489 7204 2071 Size: 4418 715 4750 Fragments arranged by size: 4750 4418 715 AclI AA'CG_TT Cuts at: 1563 8010 8383 1563 Size: 6447 373 3063 Fragments arranged by size: 6447 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 3031 3491 4002 4230 5027 5036 5940 Size: 1317 82 460 511 228 797 9 904 Cuts at: 5940 6163 7946 9330 9598 1632 Size: 223 1783 1384 268 1917 Fragments arranged by size: 1917 1783 1384 1317 904 797 511 460 268 228 223 82 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9686 AflIII A'CryG_T Cuts at: 1203 3570 8782 9759 1203 Size: 2367 5212 977 1327 Fragments arranged by size: 5212 2367 1327 977 AgeI A'CCGG_T Cuts at: 8714 8714 Size: 9883 AhdI GACnn_n'nnGTC Cuts at: 4742 8612 4742 Size: 3870 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9883 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6422 1184 Size: 5238 4645 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 9851 AlwNI CAG_nnn'CTG Cuts at: 2581 3752 3820 4633 7048 7090 7626 9198 2581 Size: 1171 68 813 2415 42 536 1572 3266 Fragments arranged by size: 3266 2415 1572 1171 813 536 68 42 ApaI G_GGCC'C Cuts at: 3063 3063 Size: 9883 ApaLI G'TGCA_C Cuts at: 7940 9096 7940 Size: 1156 8727 ApoI r'AATT_y Cuts at: 1493 2090 2323 2678 4517 4781 4886 5732 6215 Size: 597 233 355 1839 264 105 846 483 Cuts at: 6215 7192 7418 7570 9679 1493 Size: 977 226 152 2109 1697 Fragments arranged by size: 2109 1839 1697 977 846 597 483 355 264 233 226 152 105 AseI AT'TA_AT Cuts at: 160 1032 8437 160 Size: 872 7405 1606 Fragments arranged by size: 7405 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9883 AvaI C'yCGr_G Cuts at: 1870 1943 2076 3632 5036 6273 6438 7208 1870 Size: 73 133 1556 1404 1237 165 770 4545 Fragments arranged by size: 4545 1556 1404 1237 770 165 133 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2339 2372 2339 Size: 33 9850 BamHI G'GATC_C Cuts at: 3225 6553 7213 7580 3225 Size: 3328 660 367 5528 Fragments arranged by size: 5528 3328 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 3121 3764 4270 6489 Size: 325 217 627 284 1050 643 506 2219 Cuts at: 6489 7204 8659 618 Size: 715 1455 1842 Fragments arranged by size: 2219 1842 1455 1050 715 643 627 506 325 284 217 BanII G_rGCy'C Cuts at: 1391 2826 3063 3331 3549 3702 3804 3996 5269 Size: 1435 237 268 218 153 102 192 1273 Cuts at: 5269 7202 1391 Size: 1933 4072 Fragments arranged by size: 4072 1933 1435 1273 268 237 218 192 153 102 BbeI G_GCGC'C Cuts at: 3768 3768 Size: 9883 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2780 3990 4815 5077 928 Size: 971 881 1210 825 262 5734 Fragments arranged by size: 5734 1210 971 881 825 262 BbvCI CC'TCA_GC Cuts at: 2031 2745 3821 2031 Size: 714 1076 8093 Fragments arranged by size: 8093 1076 714 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3542 3576 8074 8108 1903 Size: 34 1605 34 4498 34 3678 Fragments arranged by size: 4498 3678 1605 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2609 6422 7775 8985 2609 Size: 3813 1353 1210 3507 Fragments arranged by size: 3813 3507 1353 1210 BclI T'GATC_A Cuts at: 1548 2143 1548 Size: 595 9288 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2492 2501 6755 Size: 107 122 71 631 605 820 9 4254 Cuts at: 6755 6771 8494 136 Size: 16 1723 1525 Fragments arranged by size: 4254 1723 1525 820 631 605 122 107 71 16 9 BglII A'GATC_T Cuts at: 3285 5448 6501 6643 9878 3285 Size: 2163 1053 142 3235 3290 Fragments arranged by size: 3290 3235 2163 1053 142 BlpI GC'TnA_GC Cuts at: 5393 7300 5393 Size: 1907 7976 Bme1580I G_kGCm'C Cuts at: 1790 3063 5600 5669 7944 9100 1790 Size: 1273 2537 69 2275 1156 2573 Fragments arranged by size: 2573 2537 2275 1273 1156 69 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2820 2909 6434 Size: 416 53 83 399 24 1403 89 3525 Cuts at: 6434 6745 7033 7614 8572 442 Size: 311 288 581 958 1753 Fragments arranged by size: 3525 1753 1403 958 581 416 399 311 288 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3459 1056 Size: 2403 7480 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2493 2732 2984 3729 4710 5357 6459 Size: 1073 181 239 252 745 981 647 1102 Cuts at: 6459 6745 6902 8543 1239 Size: 286 157 1641 2579 Fragments arranged by size: 2579 1641 1102 1073 981 745 647 286 252 239 181 157 Bpu10I CC'TnA_GC Cuts at: 2031 2745 3543 3821 2031 Size: 714 798 278 8093 Fragments arranged by size: 8093 798 714 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3341 3533 3950 4175 4479 4808 5093 8008 8873 Size: 192 417 225 304 329 285 2915 865 Cuts at: 8873 9171 3341 Size: 298 4053 Fragments arranged by size: 4053 2915 865 417 329 304 298 285 225 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5474 6041 8546 882 Size: 617 3975 567 2505 2219 Fragments arranged by size: 3975 2505 2219 617 567 BsaAI yAC'GTr Cuts at: 493 1204 6811 7674 493 Size: 711 5607 863 2702 Fragments arranged by size: 5607 2702 863 711 BsaBI GATnn'nnATC Cuts at: 5253 9877 5253 Size: 4624 5259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3765 4572 5536 7690 Size: 53 83 186 936 2232 807 964 2154 Cuts at: 7690 8072 8707 275 Size: 382 635 1451 Fragments arranged by size: 2232 2154 1451 964 936 807 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5415 6486 8316 8714 8988 9135 9546 5415 Size: 1071 1830 398 274 147 411 5752 Fragments arranged by size: 5752 1830 1071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 3149 3179 5108 Size: 30 165 30 1302 30 414 30 1929 Cuts at: 5108 5138 6457 6487 1178 Size: 30 1319 30 4574 Fragments arranged by size: 4574 1929 1319 1302 414 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2117 2132 2135 2138 2141 2144 2222 Size: 862 69 15 3 3 3 3 78 Cuts at: 2222 2333 2414 2496 2528 2531 2549 3542 3986 Size: 111 81 82 32 3 18 993 444 Cuts at: 3986 4079 4354 4874 5459 5957 6173 6350 1186 Size: 93 275 520 585 498 216 177 4719 Fragments arranged by size: 4719 993 862 585 520 498 444 275 216 177 111 93 82 81 78 69 32 18 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3062 3216 3593 5049 6622 6745 6828 6951 6957 Size: 154 377 1456 1573 123 83 123 6 Cuts at: 6957 7109 9086 9582 3062 Size: 152 1977 496 3363 Fragments arranged by size: 3363 1977 1573 1456 496 377 154 152 123 123 83 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4130 4193 4816 5853 1163 Size: 573 2394 63 623 1037 5193 Fragments arranged by size: 5193 2394 1037 623 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7333 8094 9122 1540 Size: 275 186 5332 761 1028 2301 Fragments arranged by size: 5332 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3549 7944 8029 9100 3549 Size: 4395 85 1071 4332 Fragments arranged by size: 4395 4332 1071 85 BsmI GAATG_Cn' Cuts at: 2977 4338 6667 7394 7487 2977 Size: 1361 2329 727 93 5373 Fragments arranged by size: 5373 2329 1361 727 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4750 4750 Size: 9883 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2826 3063 3331 3549 3702 3804 3996 Size: 399 1036 237 268 218 153 102 192 Cuts at: 3996 5269 5600 5669 7202 7944 8029 9100 1391 Size: 1273 331 69 1533 742 85 1071 2174 Fragments arranged by size: 2174 1533 1273 1071 1036 742 399 331 268 237 218 192 153 102 85 69 BspEI T'CCGG_A Cuts at: 5415 9546 5415 Size: 4131 5752 BspHI T'CATG_A Cuts at: 5088 5805 7772 9502 5088 Size: 717 1967 1730 5469 Fragments arranged by size: 5469 1967 1730 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2892 3660 3805 4282 5852 6610 7238 844 Size: 2048 768 145 477 1570 758 628 3489 Fragments arranged by size: 3489 2048 1570 768 758 628 477 145 BsrBI CCG'CTC Cuts at: 1394 1823 2907 4480 5430 7770 1394 Size: 429 1084 1573 950 2340 3507 Fragments arranged by size: 3507 2340 1573 1084 950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4353 4932 5518 5523 5578 6702 7140 Size: 1183 3105 579 586 5 55 1124 438 Cuts at: 7140 8378 8552 65 Size: 1238 174 1396 Fragments arranged by size: 3105 1396 1238 1183 1124 586 579 438 174 55 5 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 4122 5714 8527 8714 1700 Size: 110 548 1764 1592 2813 187 2869 Fragments arranged by size: 2869 2813 1764 1592 548 187 110 BsrGI T'GTAC_A Cuts at: 96 3573 4606 96 Size: 3477 1033 5373 Fragments arranged by size: 5373 3477 1033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9883 BssSI C'ACGA_G Cuts at: 3674 7943 8955 3674 Size: 4269 1012 4602 Fragments arranged by size: 4602 4269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5219 6269 7048 794 Size: 4425 1050 779 3629 Fragments arranged by size: 4425 3629 1050 779 BstBI TT'CG_AA Cuts at: 6219 8687 6219 Size: 2468 7415 BstEII G'GTnAC_C Cuts at: 1184 5056 1184 Size: 3872 6011 BstXI CCAn_nnnn'nTGG Cuts at: 3346 3593 6753 3346 Size: 247 3160 6476 Fragments arranged by size: 6476 3160 247 BstYI r'GATC_y Cuts at: 1972 2961 3225 3285 5010 5448 6501 6553 6643 Size: 989 264 60 1725 438 1053 52 90 Cuts at: 6643 7213 7580 7965 7982 9434 9520 9532 9878 Size: 570 367 385 17 1452 86 12 346 Cuts at: 9878 1972 Size: 1977 Fragments arranged by size: 1977 1725 1452 1053 989 570 438 385 367 346 264 90 86 60 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2487 2499 3693 4134 6051 6258 513 Size: 552 1422 12 1194 441 1917 207 4138 Fragments arranged by size: 4138 1917 1422 1194 552 441 207 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7164 487 Size: 683 56 5938 3206 Fragments arranged by size: 5938 3206 683 56 BtsI GCAGTG_nn' Cuts at: 2227 3203 5220 5600 5794 6790 6805 7049 7399 Size: 976 2017 380 194 996 15 244 350 Cuts at: 7399 8193 8213 2227 Size: 794 20 3897 Fragments arranged by size: 3897 2017 996 976 794 380 350 244 194 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9883 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9848 DraI TTT'AAA Cuts at: 3924 7187 3924 Size: 3263 6620 DraIII CAC_nnn'GTG Cuts at: 4108 4384 5064 6198 4108 Size: 276 680 1134 7793 Fragments arranged by size: 7793 1134 680 276 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5708 8890 809 Size: 507 4392 3182 1802 Fragments arranged by size: 4392 3182 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 3418 3609 Size: 54 1425 50 275 18 421 1167 191 Cuts at: 3609 3711 4137 4179 4671 5759 6840 7330 8219 Size: 102 426 42 492 1088 1081 490 889 Cuts at: 8219 9848 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1167 1088 1081 889 492 490 426 421 275 191 102 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 7330 1537 Size: 275 5518 4090 Fragments arranged by size: 5518 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2255 2712 3480 4470 5325 6970 7813 Size: 741 161 457 768 990 855 1645 843 Cuts at: 7813 1353 Size: 3423 Fragments arranged by size: 3423 1645 990 855 843 768 741 457 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2614 7052 7087 8470 8844 8990 Size: 1064 138 1025 4438 35 1383 374 146 Cuts at: 8990 387 Size: 1280 Fragments arranged by size: 4438 1383 1280 1064 1025 374 146 138 35 EcoICRI GAG'CTC Cuts at: 3547 3547 Size: 9883 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2493 2732 2949 2984 3031 3491 Size: 393 680 181 239 217 35 47 460 Cuts at: 3491 3729 4002 4230 4710 5027 5036 5357 5940 Size: 238 273 228 480 317 9 321 583 Cuts at: 5940 6163 6459 6745 6902 7946 8543 9330 9598 Size: 223 296 286 157 1044 597 787 268 Cuts at: 9598 1239 Size: 1524 Fragments arranged by size: 1524 1044 787 680 597 583 480 460 393 321 317 296 286 273 268 239 238 228 223 217 181 157 47 35 9 EcoNI CCTnn'n_nnAGG Cuts at: 2315 4989 6561 2315 Size: 2674 1572 5637 Fragments arranged by size: 5637 2674 1572 EcoO109I rG'GnC_Cy Cuts at: 2494 3059 3088 3539 4768 4982 4995 5075 5410 Size: 565 29 451 1229 214 13 80 335 Cuts at: 5410 6277 6591 2494 Size: 867 314 5786 Fragments arranged by size: 5786 1229 867 565 451 335 314 214 80 29 13 EcoRI G'AATT_C Cuts at: 6215 7192 7570 6215 Size: 977 378 8528 Fragments arranged by size: 8528 977 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6388 6388 Size: 9883 FspI TGC'GCA Cuts at: 8389 8389 Size: 9883 HaeII r_GCGC'y Cuts at: 1678 3768 9030 1678 Size: 2090 5262 2531 Fragments arranged by size: 5262 2531 2090 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 3353 3385 4728 Size: 32 135 32 950 32 799 32 1343 Cuts at: 4728 4760 5108 5140 6226 6258 8524 8556 8598 Size: 32 348 32 1086 32 2266 32 42 Cuts at: 8598 8630 1373 Size: 32 2626 Fragments arranged by size: 2626 2266 1343 1086 950 799 348 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4660 4924 5463 7227 7473 669 Size: 988 281 2722 264 539 1764 246 3079 Fragments arranged by size: 3079 2722 1764 988 539 281 264 246 HindIII A'AGCT_T Cuts at: 748 2606 748 Size: 1858 8025 HpaI GTT'AAC Cuts at: 5463 7473 5463 Size: 2010 7873 KasI G'GCGC_C Cuts at: 3764 3764 Size: 9883 KpnI G_GTAC'C Cuts at: 2075 6493 7208 2075 Size: 4418 715 4750 Fragments arranged by size: 4750 4418 715 MfeI C'AATT_G Cuts at: 4255 7482 4255 Size: 3227 6656 MluI A'CGCG_T Cuts at: 9759 9759 Size: 9883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3032 6100 7026 8997 9181 1305 Size: 1727 3068 926 1971 184 2007 Fragments arranged by size: 3068 2007 1971 1727 926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 3611 3713 4139 4181 Size: 54 1425 343 421 1358 102 426 42 Cuts at: 4181 4673 6842 10 Size: 492 2169 3051 Fragments arranged by size: 3051 2169 1425 1358 492 426 421 343 102 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 3174 3344 6147 7133 7841 8200 8359 Size: 648 2008 170 2803 986 708 359 159 Cuts at: 8359 518 Size: 2042 Fragments arranged by size: 2803 2042 2008 986 708 648 359 170 159 MspA1I CmG'CkG Cuts at: 1606 3220 3670 3695 4633 5053 5658 5968 6312 Size: 1614 450 25 938 420 605 310 344 Cuts at: 6312 7087 7299 7976 9124 9369 1606 Size: 775 212 677 1148 245 2120 Fragments arranged by size: 2120 1614 1148 938 775 677 605 450 420 344 310 245 212 25 NarI GG'CG_CC Cuts at: 3765 3765 Size: 9883 NcoI C'CATG_G Cuts at: 513 1065 2487 2499 4134 6051 6258 513 Size: 552 1422 12 1635 1917 207 4138 Fragments arranged by size: 4138 1917 1635 1422 552 207 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 9883 NheI G'CTAG_C Cuts at: 1052 3455 1052 Size: 2403 7480 NotI GC'GGCC_GC Cuts at: 7330 7330 Size: 9883 NspI r_CATG'y Cuts at: 1132 1229 3574 4850 6146 7241 8786 1132 Size: 97 2345 1276 1296 1095 1545 2229 Fragments arranged by size: 2345 2229 1545 1296 1276 1095 97 PasI CC'CwG_GG Cuts at: 1348 3091 5128 5542 1348 Size: 1743 2037 414 5689 Fragments arranged by size: 5689 2037 1743 414 PciI A'CATG_T Cuts at: 3570 8782 3570 Size: 5212 4671 PflMI CCAn_nnn'nTGG Cuts at: 1108 3373 6145 6198 6259 7023 1108 Size: 2265 2772 53 61 764 3968 Fragments arranged by size: 3968 2772 2265 764 61 53 PfoI T'CCnGG_A Cuts at: 2454 2611 3000 4713 4764 7164 2454 Size: 157 389 1713 51 2400 5173 Fragments arranged by size: 5173 2400 1713 389 157 51 PmeI GTTT'AAAC Cuts at: 7187 7187 Size: 9883 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 7952 7984 9465 9497 4694 Size: 32 3226 32 1481 32 5080 Fragments arranged by size: 5080 3226 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4768 4982 4995 5075 5410 6277 6591 4768 Size: 214 13 80 335 867 314 8060 Fragments arranged by size: 8060 867 335 314 214 80 13 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9883 PsiI TTA'TAA Cuts at: 7453 7453 Size: 9883 PspOMI G'GGCC_C Cuts at: 3059 3059 Size: 9883 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4137 4169 4137 Size: 32 9851 PstI C_TGCA'G Cuts at: 830 3789 5263 5929 6311 7053 7235 830 Size: 2959 1474 666 382 742 182 3478 Fragments arranged by size: 3478 2959 1474 742 666 382 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9883 PvuII CAG'CTG Cuts at: 3220 4633 5053 3220 Size: 1413 420 8050 Fragments arranged by size: 8050 1413 420 SacI G_AGCT'C Cuts at: 3549 3549 Size: 9883 SacII CC_GC'GG Cuts at: 3696 3696 Size: 9883 SalI G'TCGA_C Cuts at: 1936 7225 1936 Size: 5289 4594 SanDI GG'GwC_CC Cuts at: 4995 5075 4995 Size: 80 9803 SapI GCTCTTCn'nnn_ Cuts at: 4470 5325 4470 Size: 855 9028 SbfI CC_TGCA'GG Cuts at: 5263 7235 5263 Size: 1972 7911 ScaI AGT'ACT Cuts at: 8131 9843 8131 Size: 1712 8171 SexAI A'CCwGG_T Cuts at: 4104 4104 Size: 9883 SfcI C'TryA_G Cuts at: 826 1045 2965 3785 4237 5259 5925 6307 7049 Size: 219 1920 820 452 1022 666 382 742 Cuts at: 7049 7231 8366 9047 9238 826 Size: 182 1135 681 191 1471 Fragments arranged by size: 1920 1471 1135 1022 820 742 681 666 452 382 219 191 182 SfiI GGCCn_nnn'nGGCC Cuts at: 2492 2492 Size: 9883 SfoI GGC'GCC Cuts at: 3766 3766 Size: 9883 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9883 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9883 SmaI CCC'GGG Cuts at: 1872 6275 6440 7210 1872 Size: 4403 165 770 4545 Fragments arranged by size: 4545 4403 770 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 3356 3548 3965 4190 4494 4823 Size: 197 926 1413 192 417 225 304 329 Cuts at: 4823 5036 5108 7987 8888 9150 820 Size: 213 72 2879 901 262 1553 Fragments arranged by size: 2879 1553 1413 926 901 417 329 304 262 225 213 197 192 72 SnaBI TAC'GTA Cuts at: 493 7674 493 Size: 7181 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9883 SphI G_CATG'C Cuts at: 1132 7241 1132 Size: 6109 3774 SspI AAT'ATT Cuts at: 5 52 7807 5 Size: 47 7755 2081 Fragments arranged by size: 7755 2081 47 StuI AGG'CCT Cuts at: 1517 4235 4955 1517 Size: 2718 720 6445 Fragments arranged by size: 6445 2718 720 StyI C'CwwG_G Cuts at: 513 1065 2487 2499 3702 3714 4134 4215 4327 Size: 552 1422 12 1203 12 420 81 112 Cuts at: 4327 6051 6258 6721 7322 513 Size: 1724 207 463 601 3074 Fragments arranged by size: 3074 1724 1422 1203 601 552 463 420 207 112 81 12 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3039 6015 7910 7927 8080 8265 Size: 1255 738 721 2976 1895 17 153 185 Cuts at: 8265 8710 325 Size: 445 1498 Fragments arranged by size: 2976 1895 1498 1255 738 721 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3573 4606 4878 Size: 274 80 33 51 1443 1596 1033 272 Cuts at: 4878 8129 9841 96 Size: 3251 1712 138 Fragments arranged by size: 3251 1712 1596 1443 1033 274 272 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3194 3226 6109 6141 6193 6225 9843 9875 3194 Size: 32 2883 32 52 32 3618 32 3202 Fragments arranged by size: 3618 3202 2883 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9883 XbaI T'CTAG_A Cuts at: 7219 7219 Size: 9883 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 4970 5068 6773 7162 2092 Size: 2878 98 1705 389 4813 Fragments arranged by size: 4813 2878 1705 389 98 XhoI C'TCGA_G Cuts at: 1943 5036 1943 Size: 3093 6790 XmaI C'CCGG_G Cuts at: 1870 6273 6438 7208 1870 Size: 4403 165 770 4545 Fragments arranged by size: 4545 4403 770 165 XmnI GAAnn'nnTTC Cuts at: 8012 8012 Size: 9883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7691 276 Size: 53 83 186 936 6157 2468 Fragments arranged by size: 6157 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SrfI SwaI