(Circular) (Six-base) MAPSORT of: pFN21AB5030.seq Check: 70 from: 1 to: 5557 pFN21AB5030 5557 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2346 2346 Size: 5557 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 2900 1937 Size: 262 701 4594 Fragments arranged by size: 4594 701 262 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2145 4456 5433 1203 Size: 942 2311 977 1327 Fragments arranged by size: 2311 1327 977 942 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 4543 AlwNI CAG_nnn'CTG Cuts at: 3300 4872 3300 Size: 1572 3985 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2483 2866 3092 3244 5353 1493 Size: 990 383 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 383 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2882 1870 Size: 73 95 844 4545 Fragments arranged by size: 4545 844 95 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2471 2878 4333 618 Size: 325 217 627 387 297 407 1455 1842 Fragments arranged by size: 1842 1455 627 407 387 325 297 217 BanII G_rGCy'C Cuts at: 1391 2876 1391 Size: 1485 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 928 Size: 971 702 3884 Fragments arranged by size: 3884 971 702 BbvCI CC'TCA_GC Cuts at: 2724 2724 Size: 5557 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2044 2078 3748 3782 1903 Size: 34 107 34 1670 34 3678 Fragments arranged by size: 3678 1670 107 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BfrBI ATG'CAT Cuts at: 2602 2602 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4168 136 Size: 107 122 71 631 605 2496 1525 Fragments arranged by size: 2496 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 3618 4774 1790 Size: 1828 1156 2573 Fragments arranged by size: 2573 1828 1156 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 4729 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4246 442 Size: 416 53 83 399 24 1871 958 1753 Fragments arranged by size: 1871 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 4217 1239 Size: 1052 1926 2579 Fragments arranged by size: 2579 1926 1052 Bpu10I CC'TnA_GC Cuts at: 2724 2724 Size: 5557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 3682 4547 4845 2570 Size: 1112 865 298 3282 Fragments arranged by size: 3282 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 4220 882 Size: 617 519 2202 2219 Fragments arranged by size: 2219 2202 617 519 BsaAI yAC'GTr Cuts at: 493 1204 2539 3348 493 Size: 711 1335 809 2702 Fragments arranged by size: 2702 1335 809 711 BsaBI GATnn'nnATC Cuts at: 2246 5551 2246 Size: 3305 2252 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5332 Fragments arranged by size: 5332 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4617 BseYI C'CCAG_C Cuts at: 2030 2138 2512 2667 4760 5256 2030 Size: 108 374 155 2093 496 2331 Fragments arranged by size: 2331 2093 496 374 155 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 1163 Size: 573 825 4159 Fragments arranged by size: 4159 825 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3768 4796 1540 Size: 275 186 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 2559 2691 3618 3703 4774 2538 Size: 21 132 927 85 1071 3321 Fragments arranged by size: 3321 1071 927 132 85 21 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 BsmBI CGTCTCn'nnnn_ Cuts at: 2785 2785 Size: 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2538 2559 2691 2876 3618 3703 4774 Size: 399 748 21 132 185 742 85 1071 Cuts at: 4774 1391 Size: 2174 Fragments arranged by size: 2174 1071 748 742 399 185 132 85 21 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2912 844 Size: 1502 566 3489 Fragments arranged by size: 3489 1502 566 BsrBI CCG'CTC Cuts at: 1394 1823 2445 3444 1394 Size: 429 622 999 3507 Fragments arranged by size: 3507 999 622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4201 4388 1700 Size: 110 2391 187 2869 Fragments arranged by size: 2869 2391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2644 2416 Size: 228 5329 BstYI r'GATC_y Cuts at: 1972 2413 2887 3254 3639 3656 5108 5194 5206 Size: 441 474 367 385 17 1452 86 12 Cuts at: 5206 5552 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 474 441 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2071 2098 2736 513 Size: 552 939 67 27 638 3334 Fragments arranged by size: 3334 939 638 552 67 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3073 3867 3887 2689 Size: 384 794 20 4359 Fragments arranged by size: 4359 794 384 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2384 4564 809 Size: 507 1068 2180 1802 Fragments arranged by size: 2180 1802 1068 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 3004 3893 Size: 54 1425 50 275 18 804 370 889 Cuts at: 3893 5522 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 804 370 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2743 3487 1353 Size: 1390 744 3423 Fragments arranged by size: 3423 1390 744 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4144 4518 4664 387 Size: 1064 138 435 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 3620 4217 5004 5272 1239 Size: 393 659 1329 597 787 268 1524 Fragments arranged by size: 1524 1329 787 659 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2608 2728 2608 Size: 120 5437 EcoRI G'AATT_C Cuts at: 2483 2866 3244 2483 Size: 383 378 4796 Fragments arranged by size: 4796 383 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 2818 2788 Size: 30 5527 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 2220 4704 1678 Size: 542 2484 2531 Fragments arranged by size: 2531 2484 542 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 10 Size: 54 1425 343 804 2931 Fragments arranged by size: 2931 1425 804 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 3515 3874 4033 518 Size: 648 1227 1122 359 159 2042 Fragments arranged by size: 2042 1227 1122 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2632 2671 2701 2973 3650 4798 5043 Size: 494 532 39 30 272 677 1148 245 Cuts at: 5043 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 532 494 272 245 39 30 NcoI C'CATG_G Cuts at: 513 1065 2004 2071 513 Size: 552 939 67 3999 Fragments arranged by size: 3999 939 552 67 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NsiI A_TGCA'T Cuts at: 2604 2604 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2602 2915 4460 1132 Size: 97 1373 313 1545 2229 Fragments arranged by size: 2229 1545 1373 313 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 2704 1108 Size: 1593 3 3961 Fragments arranged by size: 3961 1593 3 PfoI T'CCnGG_A Cuts at: 2268 2268 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PmlI CAC'GTG Cuts at: 2539 2539 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2051 2083 2051 Size: 32 5525 PstI C_TGCA'G Cuts at: 830 2190 2909 830 Size: 1360 719 3478 Fragments arranged by size: 3478 1360 719 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 PvuII CAG'CTG Cuts at: 2632 2671 2701 2632 Size: 39 30 5488 Fragments arranged by size: 5488 39 30 SacII CC_GC'GG Cuts at: 2101 2101 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 2060 3805 5517 2060 Size: 1745 1712 2100 Fragments arranged by size: 2100 1745 1712 SexAI A'CCwGG_T Cuts at: 2610 2610 Size: 5557 SfcI C'TryA_G Cuts at: 826 1045 2186 2905 4040 4721 4912 826 Size: 219 1141 719 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 719 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2040 2884 1872 Size: 168 844 4545 Fragments arranged by size: 4545 844 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3661 4562 4824 820 Size: 197 926 642 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 2382 2409 2996 513 Size: 552 939 67 311 27 587 3074 Fragments arranged by size: 3074 939 587 552 311 67 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 3803 5515 Size: 274 80 33 51 1443 81 1745 1712 Cuts at: 5515 96 Size: 138 Fragments arranged by size: 1745 1712 1443 274 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2038 2882 1870 Size: 168 844 4545 Fragments arranged by size: 4545 844 168 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI