(Circular) (Six-base) MAPSORT of: pFN21AE0559.seq Check: 2625 from: 1 to: 5590 pFN21AE0559 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2337 2337 Size: 5590 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2520 2911 2520 Size: 391 5199 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AfeI AGC'GCT Cuts at: 2828 2828 Size: 5590 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 2682 4489 5466 1203 Size: 1479 1807 977 1327 Fragments arranged by size: 1807 1479 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2162 1166 Size: 996 4594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 2176 2356 3333 4905 2176 Size: 180 977 1572 2861 Fragments arranged by size: 2861 1572 977 180 ApaI G_GGCC'C Cuts at: 2094 2094 Size: 5590 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2239 2520 2911 4366 Size: 325 217 627 366 86 281 391 1455 Cuts at: 4366 618 Size: 1842 Fragments arranged by size: 1842 1455 627 391 366 325 281 217 86 BanII G_rGCy'C Cuts at: 1391 2094 2224 2745 2909 1391 Size: 703 130 521 164 4072 Fragments arranged by size: 4072 703 521 164 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2592 2804 928 Size: 971 693 212 3714 Fragments arranged by size: 3714 971 693 212 BbvCI CC'TCA_GC Cuts at: 2870 2870 Size: 5590 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2311 4201 136 Size: 107 122 71 631 605 639 1890 1525 Fragments arranged by size: 1890 1525 639 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 2549 3007 2549 Size: 458 5132 Bme1580I G_kGCm'C Cuts at: 1790 2094 2244 3651 4807 1790 Size: 304 150 1407 1156 2573 Fragments arranged by size: 2573 1407 1156 304 150 BmgBI CAC'GTC Cuts at: 1318 2407 2685 1318 Size: 1089 278 4223 Fragments arranged by size: 4223 1089 278 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2096 3321 4279 Size: 416 53 83 399 24 679 1225 958 Cuts at: 4279 442 Size: 1753 Fragments arranged by size: 1753 1225 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2303 2517 2648 4250 1239 Size: 821 243 214 131 1602 2579 Fragments arranged by size: 2579 1602 821 243 214 131 Bpu10I CC'TnA_GC Cuts at: 2454 2870 2454 Size: 416 5174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2270 3715 4580 4878 2270 Size: 1445 865 298 2982 Fragments arranged by size: 2982 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 2651 4253 882 Size: 617 920 232 1602 2219 Fragments arranged by size: 2219 1602 920 617 232 BsaAI yAC'GTr Cuts at: 493 1204 2047 2233 2530 3381 493 Size: 711 843 186 297 851 2702 Fragments arranged by size: 2702 851 843 711 297 186 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 1178 Size: 30 165 30 1341 30 3994 Fragments arranged by size: 3994 1341 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 4107 BseYI C'CCAG_C Cuts at: 2786 4793 5289 2786 Size: 2007 496 3087 Fragments arranged by size: 3087 2007 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 4201 Fragments arranged by size: 4201 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2745 3651 3736 4807 2745 Size: 906 85 1071 3528 Fragments arranged by size: 3528 1071 906 85 BsiWI C'GTAC_G Cuts at: 2233 2233 Size: 5590 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2224 2244 2745 2909 3651 3736 Size: 399 304 130 20 501 164 742 85 Cuts at: 3736 4807 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 501 399 304 164 130 85 20 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 2769 3479 5209 2769 Size: 710 1730 3150 Fragments arranged by size: 3150 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2337 2945 844 Size: 1289 204 608 3489 Fragments arranged by size: 3489 1289 608 204 BsrBI CCG'CTC Cuts at: 1394 1823 2062 2880 3477 1394 Size: 429 239 818 597 3507 Fragments arranged by size: 3507 818 597 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2622 2920 3287 3672 3689 5141 5227 5239 Size: 650 298 367 385 17 1452 86 12 Cuts at: 5239 5585 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 650 385 367 346 298 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2275 2275 Size: 5590 BtgI C'CryG_G Cuts at: 513 1065 2004 2309 2661 2811 513 Size: 552 939 305 352 150 3292 Fragments arranged by size: 3292 939 552 352 305 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2388 2423 529 Size: 35 1824 35 3696 Fragments arranged by size: 3696 1824 35 35 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2411 4597 809 Size: 507 1095 2186 1802 Fragments arranged by size: 2186 1802 1095 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555 Size: 54 1425 50 275 18 1207 889 1629 Cuts at: 5555 8 Size: 43 Fragments arranged by size: 1629 1425 1207 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 3520 1353 Size: 1229 938 3423 Fragments arranged by size: 3423 1229 938 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2743 2743 Size: 5590 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2303 2517 2648 3653 4250 5037 Size: 393 428 243 214 131 1005 597 787 Cuts at: 5037 5305 1239 Size: 268 1524 Fragments arranged by size: 1524 1005 787 597 428 393 268 243 214 131 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2770 2651 Size: 119 5471 EcoO109I rG'GnC_Cy Cuts at: 2056 2090 2091 2463 2514 2056 Size: 34 1 372 51 5132 Fragments arranged by size: 5132 372 51 34 1 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2821 2821 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2644 2830 4737 1678 Size: 966 186 1907 2531 Fragments arranged by size: 2531 1907 966 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2524 2915 2524 Size: 391 5199 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 4704 4888 1305 Size: 1035 2364 184 2007 Fragments arranged by size: 2364 2007 1035 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3768 Fragments arranged by size: 3768 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 3548 3907 4066 518 Size: 648 996 1386 359 159 2042 Fragments arranged by size: 2042 1386 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3006 3683 4831 5076 1606 Size: 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2661 513 Size: 552 939 657 3442 Fragments arranged by size: 3442 939 657 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 2437 1108 Size: 1068 261 4261 Fragments arranged by size: 4261 1068 261 PfoI T'CCnGG_A Cuts at: 2016 2478 2625 2016 Size: 462 147 4981 Fragments arranged by size: 4981 462 147 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PmlI CAC'GTG Cuts at: 2530 2530 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2463 2514 2056 Size: 407 51 5132 Fragments arranged by size: 5132 407 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2090 2090 Size: 5590 PstI C_TGCA'G Cuts at: 830 2100 2867 2942 830 Size: 1270 767 75 3478 Fragments arranged by size: 3478 1270 767 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 SacI G_AGCT'C Cuts at: 2745 2745 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SanDI GG'GwC_CC Cuts at: 2056 2056 Size: 5590 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 2072 3838 5550 2072 Size: 1766 1712 2112 Fragments arranged by size: 2112 1766 1712 SfcI C'TryA_G Cuts at: 826 1045 2096 2863 2938 4073 4754 4945 826 Size: 219 1051 767 75 1135 681 191 1471 Fragments arranged by size: 1471 1135 1051 767 681 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 3694 4595 4857 820 Size: 197 926 306 1445 901 262 1553 Fragments arranged by size: 1553 1445 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 2233 3381 493 Size: 1740 1148 2702 Fragments arranged by size: 2702 1740 1148 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2661 3029 513 Size: 552 939 657 368 3074 Fragments arranged by size: 3074 939 657 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2152 2573 3617 3634 3787 3972 4417 Size: 1255 572 421 1044 17 153 185 445 Cuts at: 4417 325 Size: 1498 Fragments arranged by size: 1498 1255 1044 572 445 421 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 3836 5548 Size: 274 80 33 51 1443 93 1766 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1766 1712 1443 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 5550 5582 2744 Size: 32 2774 32 2752 Fragments arranged by size: 2774 2752 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2668 2809 2668 Size: 141 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI