Restriction map for
pFN21AB7227
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI Ksp632I MaeIII MamI
MboI MflI MluI MluNI MroI
MscI MspI MvaI MwoI NaeI
NarI NciI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI Sau96I SbfI ScaI ScrFI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI TseI
Tsp45I TspRI Tth111I Van91I XcmI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 433 CGCG CG^CG
AcsI 124 AAATTC [GA]^AATT[CT]
AluI 174 AGCT AG^CT
Aor51HI 353 AGCGCT AGC^GCT
ApoI 124 AAATTC [GA]^AATT[CT]
AvaI 323 CTCGAG C^[CT]CG[GA]G
BanII 309 GGGCTC G[GA]GC[CT]^C
BmyI 309 GGGCTC G[AGT]GC[ACT]^C
BpmI 203 CTGGAG CTGGAG(16/14)
BsmFI 537 GTCCC GGGAC(10/14)
BsoBI 323 CTCGAG C^[CT]CG[GA]G
Bsp1286I 309 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 344 TGTACA T^GTACA
BsrDI 230 CATTGC GCAATG(2/0)
BsrGI 344 TGTACA T^GTACA
BstUI 433 CGCG CG^CG
BstXI 444 CCATCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 354 GCGC GCG^C
Eco47III 353 AGCGCT AGC^GCT
EcoT14I 129 CCATGG C^C[AT][AT]GG
HaeII 353 AGCGCT [GA]GCGC^[CT]
HhaI 354 GCGC GCG^C
HinP1I 354 GCGC G^CGC
KspI 432 CCGCGG CCGC^GG
MaeII 633 ACGT A^CGT
MfeI 14 CAATTG C^AATTG
MseI 80 TTAA T^TAA
MspA1I 432 CCGCGG C[AC]G^C[GT]G
MunI 14 CAATTG C^AATTG
MvnI 433 CGCG CG^CG
NcoI 129 CCATGG C^CATGG
NdeI 276 CATATG CA^TATG
NlaIV 310 GGCTCC GG[ACGT]^[ACGT]CC
PaeR7I 323 CTCGAG C^TCGAG
SacII 432 CCGCGG CCGC^GG
SmlI 323 CTCGAG C^T[CT][GA]AG
SpeI 498 ACTAGT A^CTAGT
SspBI 344 TGTACA T^GTACA
StyI 129 CCATGG C^C[AT][AT]GG
Tru9I 80 TTAA T^TAA
XbaI 118 TCTAGA T^CTAGA
XhoI 323 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 432 CCGC CCGC(-3/-1)
434 GCGG
AfaI 250 GTAC GT^AC
345 GTAC
BfaI 119 CTAG C^TAG
499 CTAG
BsmAI 206 GAGAC GTCTC(1/5)
326 GAGAC
BsrI 202 ACTGG ACTGG(1/-1)
107 CCAGT
BstF5I 445 CATCC GGATG(2/0)
585 CATCC
DdeI 339 CTTAG C^T[ACGT]AG
573 CTTAG
FokI 445 CATCC GGATG(9/13)
585 CATCC
HinfI 166 GAATC G^A[ACGT]TC
262 GATTC
HphI 169 TCACC GGTGA(8/7)
466 TCACC
MaeI 119 CTAG C^TAG
499 CTAG
MboII 475 GAAGA GAAGA(8/7)
418 TCTTC
MslI 272 CATTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
445 CATCCTTGTG
RsaI 250 GTAC GT^AC
345 GTAC
TfiI 166 GAATC G^A[AT]TC
262 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BsaJI DsaI MnlI NlaIII TaqI
Tsp509I TthHB8I