(Circular) (Six-base) MAPSORT of: pFN21AE0301.seq Check: 6692 from: 1 to: 6232 pFN21AE0301 6232 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2008 2008 Size: 6232 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 3531 4359 4732 1563 Size: 1968 828 373 3063 Fragments arranged by size: 3063 1968 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 4295 5679 5947 1632 Size: 1172 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 1172 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 3165 3295 5131 6108 1203 Size: 1962 130 1836 977 1327 Fragments arranged by size: 1962 1836 1327 977 130 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2966 1184 Size: 1782 4450 AlwNI CAG_nnn'CTG Cuts at: 2023 2237 2507 2633 3401 3975 5547 2023 Size: 214 270 126 768 574 1572 2708 Fragments arranged by size: 2708 1572 768 574 270 214 126 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 3541 3767 3919 6028 1493 Size: 2048 226 152 2109 1697 Fragments arranged by size: 2109 2048 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2852 3449 3557 1870 Size: 73 909 597 108 4545 Fragments arranged by size: 4545 909 597 108 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2379 2412 2379 Size: 33 6199 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3551 1391 Size: 2160 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3103 928 Size: 971 1204 4057 Fragments arranged by size: 4057 1204 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2380 2540 4124 5334 2380 Size: 160 1584 1210 3278 Fragments arranged by size: 3278 1584 1210 160 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2449 4293 5449 1790 Size: 659 1844 1156 2573 Fragments arranged by size: 2573 1844 1156 659 BmgBI CAC'GTC Cuts at: 1318 2161 1318 Size: 843 5389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 3013 3963 Size: 416 53 83 399 24 636 960 950 Cuts at: 3963 4921 442 Size: 958 1753 Fragments arranged by size: 1753 960 958 950 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2357 1056 Size: 1301 4931 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2583 4892 1239 Size: 1203 141 2309 2579 Fragments arranged by size: 2579 2309 1203 141 Bpu10I CC'TnA_GC Cuts at: 2358 2358 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2803 4023 493 Size: 711 1599 1220 2702 Fragments arranged by size: 2702 1599 1220 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3046 4665 5063 5337 5484 5895 3046 Size: 1619 398 274 147 411 3383 Fragments arranged by size: 3383 1619 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3267 1186 Size: 2081 4151 BseYI C'CCAG_C Cuts at: 2349 5435 5931 2349 Size: 3086 496 2650 Fragments arranged by size: 3086 2650 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 5308 Fragments arranged by size: 5308 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2383 4293 4378 5449 2383 Size: 1910 85 1071 3166 Fragments arranged by size: 3166 1910 1071 85 BsmI GAATG_Cn' Cuts at: 2593 3743 3836 2593 Size: 1150 93 4989 Fragments arranged by size: 4989 1150 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2102 2102 Size: 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2383 2449 3551 4293 4378 5449 1391 Size: 399 593 66 1102 742 85 1071 2174 Fragments arranged by size: 2174 1102 1071 742 593 399 85 66 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 2946 4121 5851 2946 Size: 1175 1730 3327 Fragments arranged by size: 3327 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 3587 844 Size: 1164 1579 3489 Fragments arranged by size: 3489 1579 1164 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2994 4727 4901 65 Size: 1183 1746 1733 174 1396 Fragments arranged by size: 1746 1733 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3162 96 Size: 3066 3166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 794 Size: 1229 5003 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2688 2728 3187 3562 3929 4314 4331 5783 Size: 716 40 459 375 367 385 17 1452 Cuts at: 5783 5869 5881 6227 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 716 459 385 375 367 346 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 3082 3082 Size: 6232 BtgI C'CryG_G Cuts at: 513 1065 2724 513 Size: 552 1659 4021 Fragments arranged by size: 4021 1659 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4542 4562 3748 Size: 794 20 5418 Fragments arranged by size: 5418 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2423 5239 809 Size: 507 1107 2816 1802 Fragments arranged by size: 2816 1802 1107 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3679 4568 Size: 54 1425 50 275 18 378 1471 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1471 1425 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 4162 1353 Size: 801 2008 3423 Fragments arranged by size: 3423 2008 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 4819 5193 5339 387 Size: 1064 138 504 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 504 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2442 2583 2804 4295 4892 5679 5947 Size: 393 810 141 221 1491 597 787 268 Cuts at: 5947 1239 Size: 1524 Fragments arranged by size: 1524 1491 810 787 597 393 268 221 141 EcoO109I rG'GnC_Cy Cuts at: 2055 2913 2055 Size: 858 5374 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 5379 1678 Size: 3701 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373 Size: 32 135 32 3301 32 42 32 2626 Fragments arranged by size: 3301 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2875 3171 3576 3822 669 Size: 988 281 937 296 405 246 3079 Fragments arranged by size: 3079 988 937 405 296 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 2875 3822 2875 Size: 947 5285 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3283 5346 5530 1305 Size: 1978 2063 184 2007 Fragments arranged by size: 2063 2007 1978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 4032 Fragments arranged by size: 4032 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2948 4190 4549 4708 518 Size: 648 1782 1242 359 159 2042 Fragments arranged by size: 2042 1782 1242 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2312 2866 3071 3648 4325 5473 5718 1606 Size: 706 554 205 577 677 1148 245 2120 Fragments arranged by size: 2120 1148 706 677 577 554 245 205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5680 NdeI CA'TA_TG Cuts at: 387 2399 387 Size: 2012 4220 NheI G'CTAG_C Cuts at: 1052 2353 1052 Size: 1301 4931 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3169 3299 3590 5135 1132 Size: 97 1940 130 291 1545 2229 Fragments arranged by size: 2229 1940 1545 291 130 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 3165 3295 5131 3165 Size: 130 1836 4266 Fragments arranged by size: 4266 1836 130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PfoI T'CCnGG_A Cuts at: 2532 2532 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 2913 2055 Size: 858 5374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 2217 3802 2217 Size: 1585 4647 PstI C_TGCA'G Cuts at: 830 2118 2440 3070 3501 3584 830 Size: 1288 322 630 431 83 3478 Fragments arranged by size: 3478 1288 630 431 322 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2312 2866 3071 2312 Size: 554 205 5473 Fragments arranged by size: 5473 554 205 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 2523 4480 6192 2523 Size: 1957 1712 2563 Fragments arranged by size: 2563 1957 1712 SexAI A'CCwGG_T Cuts at: 3400 3400 Size: 6232 SfcI C'TryA_G Cuts at: 826 1045 2114 2436 3066 3497 3580 4715 5396 Size: 219 1069 322 630 431 83 1135 681 Cuts at: 5396 5587 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1069 681 630 431 322 219 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 3286 4156 5 Size: 47 3234 870 2081 Fragments arranged by size: 3234 2081 870 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 3671 513 Size: 552 2606 3074 Fragments arranged by size: 3074 2606 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2521 2770 3162 Size: 274 80 33 51 1443 544 249 392 Cuts at: 3162 4478 6190 96 Size: 1316 1712 138 Fragments arranged by size: 1712 1443 1316 544 392 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 2162 1316 Size: 846 5386 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 2294 3261 4361 2294 Size: 967 1100 4165 Fragments arranged by size: 4165 1100 967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI