(Circular) (Six-base) MAPSORT of: pFN21AB9552.seq Check: 381 from: 1 to: 6103 pFN21AB9552 6103 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2397 2397 Size: 6103 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4166 5550 5818 1632 Size: 2534 1384 268 1917 Fragments arranged by size: 2534 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2905 3846 5418 2905 Size: 941 1572 3590 Fragments arranged by size: 3590 1572 941 ApaI G_GGCC'C Cuts at: 2103 3093 3102 3294 3345 2103 Size: 990 9 192 51 4861 Fragments arranged by size: 4861 990 192 51 9 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 3412 3638 3790 5899 1493 Size: 1919 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2140 2687 3247 3428 1870 Size: 73 134 63 547 560 181 4545 Fragments arranged by size: 4545 560 547 181 134 73 63 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2291 2649 2919 3424 Size: 325 217 627 479 25 358 270 505 Cuts at: 3424 4879 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 505 479 358 325 270 217 25 BanII G_rGCy'C Cuts at: 1391 2045 2054 2064 2103 2350 2406 2666 3093 Size: 654 9 10 39 247 56 260 427 Cuts at: 3093 3102 3294 3345 3422 1391 Size: 9 192 51 77 4072 Fragments arranged by size: 4072 654 427 260 247 192 77 56 51 39 10 9 9 BbeI G_GCGC'C Cuts at: 2295 2295 Size: 6103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2747 3080 928 Size: 971 848 333 3951 Fragments arranged by size: 3951 971 848 333 BbvCI CC'TCA_GC Cuts at: 3152 3152 Size: 6103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2350 2384 4294 4328 1903 Size: 34 413 34 1910 34 3678 Fragments arranged by size: 3678 1910 413 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2533 2615 Size: 107 122 71 631 605 377 484 82 Cuts at: 2615 2917 3097 3249 4714 136 Size: 302 180 152 1465 1525 Fragments arranged by size: 1525 1465 631 605 484 377 302 180 152 122 107 82 71 BglII A'GATC_T Cuts at: 6098 6098 Size: 6103 BlpI GC'TnA_GC Cuts at: 2039 2243 2400 2405 2475 3172 3520 2039 Size: 204 157 5 70 697 348 4622 Fragments arranged by size: 4622 697 348 204 157 70 5 Bme1580I G_kGCm'C Cuts at: 1790 2103 2271 2576 2922 2974 3093 3102 3294 Size: 313 168 305 346 52 119 9 192 Cuts at: 3294 3345 4164 5320 1790 Size: 51 819 1156 2573 Fragments arranged by size: 2573 1156 819 346 313 305 192 168 119 52 51 9 BmgBI CAC'GTC Cuts at: 1318 2314 1318 Size: 996 5107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3193 3340 3376 Size: 416 53 83 399 24 1776 147 36 Cuts at: 3376 3834 4792 442 Size: 458 958 1753 Fragments arranged by size: 1776 1753 958 458 416 399 147 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2449 4763 1239 Size: 1210 2314 2579 Fragments arranged by size: 2579 2314 1210 Bpu10I CC'TnA_GC Cuts at: 2349 3152 2349 Size: 803 5300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5093 5391 4228 Size: 865 298 4940 Fragments arranged by size: 4940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2867 3043 3074 4766 882 Size: 617 1368 176 31 1692 2219 Fragments arranged by size: 2219 1692 1368 617 176 31 BsaAI yAC'GTr Cuts at: 493 1204 3894 493 Size: 711 2690 2702 Fragments arranged by size: 2702 2690 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 3910 4292 4927 Size: 53 83 186 936 759 1618 382 635 Cuts at: 4927 275 Size: 1451 Fragments arranged by size: 1618 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 1178 Size: 30 165 30 1368 30 4480 Fragments arranged by size: 4480 1368 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2072 2483 1186 Size: 841 45 411 4806 Fragments arranged by size: 4806 841 411 45 BseYI C'CCAG_C Cuts at: 2155 2416 2962 3329 5306 5802 2155 Size: 261 546 367 1977 496 2456 Fragments arranged by size: 2456 1977 546 496 367 261 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 1163 Size: 573 639 4891 Fragments arranged by size: 4891 639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2406 2694 4164 4249 5320 2244 Size: 162 288 1470 85 1071 3027 Fragments arranged by size: 3027 1470 1071 288 162 85 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2054 2064 2103 2244 2271 2350 Size: 399 255 9 10 39 141 27 79 Cuts at: 2350 2406 2576 2666 2694 2922 2974 3093 3102 Size: 56 170 90 28 228 52 119 9 Cuts at: 3102 3294 3345 3422 4164 4249 5320 1391 Size: 192 51 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 255 228 192 170 141 119 90 85 79 77 56 52 51 39 28 27 10 9 9 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 2913 3458 844 Size: 1553 516 545 3489 Fragments arranged by size: 3489 1553 545 516 BsrBI CCG'CTC Cuts at: 1394 1823 2479 3990 1394 Size: 429 656 1511 3507 Fragments arranged by size: 3507 1511 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2646 2951 4747 4934 1700 Size: 110 836 305 1796 187 2869 Fragments arranged by size: 2869 1796 836 305 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6103 BssHII G'CGCG_C Cuts at: 1925 2085 2357 1925 Size: 160 272 5671 Fragments arranged by size: 5671 272 160 BssSI C'ACGA_G Cuts at: 2852 4163 5175 2852 Size: 1311 1012 3780 Fragments arranged by size: 3780 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6103 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2711 3260 2711 Size: 549 5554 BstYI r'GATC_y Cuts at: 1972 2020 3003 3433 3800 4185 4202 5654 5740 Size: 48 983 430 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 983 430 385 367 346 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 2698 3226 3337 2698 Size: 528 111 5464 Fragments arranged by size: 5464 528 111 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 2710 2790 2979 3033 3084 Size: 552 939 42 664 80 189 54 51 Cuts at: 3084 513 Size: 3532 Fragments arranged by size: 3532 939 664 552 189 80 54 51 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2974 3619 4413 4433 2974 Size: 645 794 20 4644 Fragments arranged by size: 4644 794 645 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DraIII CAC_nnn'GTG Cuts at: 3050 3050 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2976 2982 3241 Size: 54 1425 50 275 18 1146 6 259 Cuts at: 3241 3315 3550 4439 6068 8 Size: 74 235 889 1629 43 Fragments arranged by size: 1629 1425 1146 889 275 259 235 74 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4033 1353 Size: 2680 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2133 2207 2551 2582 2986 Size: 1064 138 435 109 74 344 31 404 Cuts at: 2986 4690 5064 5210 387 Size: 1704 374 146 1280 Fragments arranged by size: 1704 1280 1064 435 404 374 344 146 138 109 74 31 EcoICRI GAG'CTC Cuts at: 2404 2404 Size: 6103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2449 4166 4763 5550 5818 1239 Size: 393 817 1717 597 787 268 1524 Fragments arranged by size: 1717 1524 817 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2099 3089 3090 3098 3099 3230 3290 3291 3341 Size: 990 1 8 1 131 60 1 50 Cuts at: 3341 3342 2099 Size: 1 4860 Fragments arranged by size: 4860 990 131 60 50 8 1 1 1 1 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 2295 2458 2634 2788 5250 1678 Size: 617 163 176 154 2462 2531 Fragments arranged by size: 2531 2462 617 176 163 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2738 2770 2771 2803 2846 Size: 32 135 32 1166 32 1 32 43 Cuts at: 2846 2878 3107 3139 4744 4776 4818 4850 1373 Size: 32 229 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 1166 229 135 43 42 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3447 3693 669 Size: 988 281 349 1160 246 3079 Fragments arranged by size: 3079 1160 988 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KasI G'GCGC_C Cuts at: 2291 2291 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2753 2874 2889 5217 5401 1305 Size: 1448 121 15 2328 184 2007 Fragments arranged by size: 2328 2007 1448 184 121 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2984 3243 10 Size: 54 1425 343 1152 259 2870 Fragments arranged by size: 2870 1425 1152 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 4061 4420 4579 518 Size: 648 1110 1785 359 159 2042 Fragments arranged by size: 2042 1785 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 2247 3086 3301 3519 4196 5344 5589 Size: 461 180 839 215 218 677 1148 245 Cuts at: 5589 1606 Size: 2120 Fragments arranged by size: 2120 1148 839 677 461 245 218 215 180 NaeI GCC'GGC Cuts at: 2953 2953 Size: 6103 NarI GG'CG_CC Cuts at: 2292 2292 Size: 6103 NcoI C'CATG_G Cuts at: 513 1065 2004 2710 2790 513 Size: 552 939 706 80 3826 Fragments arranged by size: 3826 939 706 552 80 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NgoMIV G'CCGG_C Cuts at: 2951 2951 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 3461 5006 1132 Size: 97 2232 1545 2229 Fragments arranged by size: 2232 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2557 3094 1348 Size: 1209 537 4357 Fragments arranged by size: 4357 1209 537 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 3366 1108 Size: 2258 3845 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3230 3230 Size: 6103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PspOMI G'GGCC_C Cuts at: 2099 3089 3098 3290 3341 2099 Size: 990 9 192 51 4861 Fragments arranged by size: 4861 990 192 51 9 PstI C_TGCA'G Cuts at: 830 2754 3455 830 Size: 1924 701 3478 Fragments arranged by size: 3478 1924 701 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 SacI G_AGCT'C Cuts at: 2406 2406 Size: 6103 SacII CC_GC'GG Cuts at: 3087 3087 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SexAI A'CCwGG_T Cuts at: 3046 3046 Size: 6103 SfcI C'TryA_G Cuts at: 826 1045 2750 3451 4586 5267 5458 826 Size: 219 1705 701 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 701 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3097 3249 3097 Size: 152 5951 SfoI GGC'GCC Cuts at: 2293 2293 Size: 6103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 2079 2142 3430 1872 Size: 207 63 1288 4545 Fragments arranged by size: 4545 1288 207 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 4207 5108 5370 820 Size: 197 926 2264 901 262 1553 Fragments arranged by size: 2264 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 3257 1517 Size: 1740 4363 StyI C'CwwG_G Cuts at: 513 1065 2004 2710 2790 3542 513 Size: 552 939 706 80 752 3074 Fragments arranged by size: 3074 939 752 706 552 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2667 2734 2937 4130 4147 4300 4485 Size: 1255 1087 67 203 1193 17 153 185 Cuts at: 4485 4930 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1193 1087 445 203 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4349 6061 96 Size: 274 80 33 51 1443 2372 1712 138 Fragments arranged by size: 2372 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6063 6095 6063 Size: 32 6071 Tth111I GACn'n_nGTC Cuts at: 1316 3391 1316 Size: 2075 4028 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 2077 2140 3428 1870 Size: 207 63 1288 4545 Fragments arranged by size: 4545 1288 207 63 XmnI GAAnn'nnTTC Cuts at: 2333 4232 2333 Size: 1899 4204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI SrfI SwaI XcmI