################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MluI MroI MspA1I MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1529 GGTACC G^GTACC Alw44I 539 GTGCAC G^TGCAC ApaLI 539 GTGCAC G^TGCAC Asp718I 1529 GGTACC G^GTACC AspHI 539 GTGCAC G[AT]GC[AT]^C AvaII 1857 GGTCC G^G[AT]CC BalI 289 TGGCCA TGG^CCA BbsI 1844 GTCTTC GAAGAC(2/6) BglII 1269 AGATCT A^GATCT BpmI 1310 CTCCAG CTGGAG(16/14) BpuAI 1844 GTCTTC GAAGAC(2/6) BsaWI 308 ACCGGA [AT]^CCGG[AT] BsgI 113 GTGCAG GTGCAG(16/14) BsiHKAI 539 GTGCAC G[AT]GC[AT]^C BspDI 284 ATCGAT AT^CGAT BsrDI 1286 CATTGC GCAATG(2/0) BstXI 569 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 284 ATCGAT AT^CGAT DraI 2220 TTTAAA TTT^AAA DraII 1856 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 149 GACTTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 209 CCATGG C^C[GA][CT]GG EaeI 289 TGGCCA [CT]^GGCC[GA] EarI 374 CTCTTC CTCTTC(1/4) EcoO109I 1856 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 592 GATATC GAT^ATC HapII 309 CCGG C^CGG HindIII 678 AAGCTT A^AGCTT HpaII 309 CCGG C^CGG KpnI 1529 GGTACC GGTAC^C Ksp632I 374 CTCTTC CTCTTC(1/4) MfeI 355 CAATTG C^AATTG MluNI 289 TGGCCA TGG^CCA MscI 289 TGGCCA TGG^CCA MspI 309 CCGG C^CGG MunI 355 CAATTG C^AATTG NcoI 209 CCATGG C^CATGG NdeI 317 CATATG CA^TATG PpuMI 1856 AGGTCCT [GA]G^G[AT]CC[CT] SapI 373 GCTCTTC GCTCTTC(1/4) ScaI 2047 AGTACT AGT^ACT SspI 1398 AATATT AAT^ATT XbaI 237 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 44 GTAGAC GT^[AC][GT]AC 1108 GTAGAC AlwI 1246 GATCC GGATC(4/5) 687 GGATC BanI 1529 GGTACC G^G[CT][GA]CC 1661 GGTGCC BcgI 169 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 332 GCAGAGATGTCG BclI 1274 TGATCA T^GATCA 1449 TGATCA BmyI 539 GTGCAC G[AGT]GC[ACT]^C 1662 GTGCCC BsaBI 139 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1348 GATATTTATC BsiYI 64 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2091 CCTAAGCATGG BslI 64 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2091 CCTAAGCATGG BsmI 771 GAATGC GAATGC(1/-1) 18 GCATTC Bsp1286I 539 GTGCAC G[AGT]GC[ACT]^C 1662 GTGCCC BssSI 2180 CACGAG CACGAG(-5/-1) 1958 CTCGTG BstYI 1245 AGATCC [GA]^GATC[CT] 1269 AGATCT FbaI 1274 TGATCA T^GATCA 1449 TGATCA MamI 139 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1348 GATATTTATC MflI 1245 AGATCC [GA]^GATC[CT] 1269 AGATCT MwoI 994 GCGGTGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1396 GCAATATTAGC NspI 498 ACATGC [GA]CATG^[CT] 623 GCATGT PleI 1371 GACTC GAGTC(4/5) 1637 GACTC TspRI 1029 CAGTG CA[GC]TG[ACGT][ACGT]^ 1978 CAGTG XhoII 1245 AGATCC [GA]^GATC[CT] 1269 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BciVI BfaI BsaJI BseRI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I