(Circular) (Six-base) MAPSORT of: pFN21AE3289.seq Check: 6222 from: 1 to: 6940 pFN21AE3289 6940 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4750 278 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2050 3114 4283 1937 Size: 113 1064 1169 4594 Fragments arranged by size: 4594 1169 1064 113 Acc65I G'GTAC_C Cuts at: 3534 4261 3534 Size: 727 6213 AclI AA'CG_TT Cuts at: 1563 5067 5440 1563 Size: 3504 373 3063 Fragments arranged by size: 3504 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3423 3429 3880 3941 5003 6387 6655 1632 Size: 1791 6 451 61 1062 1384 268 1917 Fragments arranged by size: 1917 1791 1384 1062 451 268 61 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6743 AflIII A'CryG_T Cuts at: 1203 5839 6816 1203 Size: 4636 977 1327 Fragments arranged by size: 4636 1327 977 AgeI A'CCGG_T Cuts at: 5771 5771 Size: 6940 AhdI GACnn_n'nnGTC Cuts at: 5669 5669 Size: 6940 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3891 1184 Size: 2707 4233 AlwNI CAG_nnn'CTG Cuts at: 4683 6255 4683 Size: 1572 5368 ApaLI G'TGCA_C Cuts at: 2544 4997 6153 2544 Size: 2453 1156 3331 Fragments arranged by size: 3331 2453 1156 ApoI r'AATT_y Cuts at: 1493 2908 3596 4012 4082 4249 4475 4627 6736 Size: 1415 688 416 70 167 226 152 2109 Cuts at: 6736 1493 Size: 1697 Fragments arranged by size: 2109 1697 1415 688 416 226 167 152 70 AseI AT'TA_AT Cuts at: 160 1032 5494 160 Size: 872 4462 1606 Fragments arranged by size: 4462 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 AvaI C'yCGr_G Cuts at: 1870 1943 4265 1870 Size: 73 2322 4545 Fragments arranged by size: 4545 2322 73 BamHI G'GATC_C Cuts at: 4270 4637 4270 Size: 367 6573 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3534 3666 4261 5716 618 Size: 325 217 627 1747 132 595 1455 1842 Fragments arranged by size: 1842 1747 1455 627 595 325 217 132 BanII G_rGCy'C Cuts at: 1391 4259 1391 Size: 2868 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3842 928 Size: 971 1943 4026 Fragments arranged by size: 4026 1943 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2163 2197 2326 2360 5131 5165 1903 Size: 34 226 34 129 34 2771 34 3678 Fragments arranged by size: 3678 2771 226 129 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2461 3931 4011 4832 6042 2461 Size: 1470 80 821 1210 3359 Fragments arranged by size: 3359 1470 1210 821 80 BclI T'GATC_A Cuts at: 1548 3279 3454 1548 Size: 1731 175 5034 Fragments arranged by size: 5034 1731 175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5551 136 Size: 107 122 71 631 605 3879 1525 Fragments arranged by size: 3879 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3274 6935 3274 Size: 3661 3279 BlpI GC'TnA_GC Cuts at: 4357 4357 Size: 6940 Bme1580I G_kGCm'C Cuts at: 1790 2548 3671 5001 6157 1790 Size: 758 1123 1330 1156 2573 Fragments arranged by size: 2573 1330 1156 1123 758 BmgBI CAC'GTC Cuts at: 1318 3127 1318 Size: 1809 5131 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2776 4671 5629 Size: 416 53 83 399 24 1359 1895 958 Cuts at: 5629 442 Size: 1753 Fragments arranged by size: 1895 1753 1359 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6940 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3300 5600 1239 Size: 2061 2300 2579 Fragments arranged by size: 2579 2300 2061 Bpu10I CC'TnA_GC Cuts at: 4097 4097 Size: 6940 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5065 5930 6228 5065 Size: 865 298 5777 Fragments arranged by size: 5777 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5603 882 Size: 617 4104 2219 Fragments arranged by size: 4104 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4731 493 Size: 711 3527 2702 Fragments arranged by size: 3527 2702 711 BsaBI GATnn'nnATC Cuts at: 2148 3357 6934 2148 Size: 1209 3577 2154 Fragments arranged by size: 3577 2154 1209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4747 5129 5764 275 Size: 53 83 186 936 3214 382 635 1451 Fragments arranged by size: 3214 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2313 5373 5771 6045 6192 6603 2313 Size: 3060 398 274 147 411 2650 Fragments arranged by size: 3060 2650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2940 2970 1178 Size: 30 165 30 1537 30 5148 Fragments arranged by size: 5148 1537 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2741 1186 Size: 838 3 714 5385 Fragments arranged by size: 5385 838 714 3 BseYI C'CCAG_C Cuts at: 6143 6639 6143 Size: 496 6444 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2139 1163 Size: 573 403 5964 Fragments arranged by size: 5964 573 403 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4390 5151 6179 1540 Size: 275 186 2389 761 1028 2301 Fragments arranged by size: 2389 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 5001 5086 6157 2548 Size: 2453 85 1071 3331 Fragments arranged by size: 3331 2453 1071 85 BsmI GAATG_Cn' Cuts at: 2023 2782 4451 4544 2023 Size: 759 1669 93 4419 Fragments arranged by size: 4419 1669 759 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2548 3671 4259 5001 5086 6157 1391 Size: 399 758 1123 588 742 85 1071 2174 Fragments arranged by size: 2174 1123 1071 758 742 588 399 85 BspEI T'CCGG_A Cuts at: 6603 6603 Size: 6940 BspHI T'CATG_A Cuts at: 4829 6559 4829 Size: 1730 5210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4295 844 Size: 3451 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4827 1394 Size: 429 3004 3507 Fragments arranged by size: 3507 3004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3290 5435 5609 65 Size: 1183 2042 2145 174 1396 Fragments arranged by size: 2145 2042 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5584 5771 1700 Size: 110 3774 187 2869 Fragments arranged by size: 3774 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6940 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6940 BssSI C'ACGA_G Cuts at: 3963 4185 5000 6012 3963 Size: 222 815 1012 4891 Fragments arranged by size: 4891 1012 815 222 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6940 BstBI TT'CG_AA Cuts at: 5744 5744 Size: 6940 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6940 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 6940 BstYI r'GATC_y Cuts at: 1972 3250 3274 4270 4637 5022 5039 6491 6577 Size: 1278 24 996 367 385 17 1452 86 Cuts at: 6577 6589 6935 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1278 996 385 367 346 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2214 513 Size: 552 939 210 5239 Fragments arranged by size: 5239 939 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6201 Fragments arranged by size: 6201 683 56 BtsI GCAGTG_nn' Cuts at: 3989 4456 5250 5270 3989 Size: 467 794 20 5659 Fragments arranged by size: 5659 794 467 20 ClaI AT'CG_AT Cuts at: 1554 2290 1554 Size: 736 6204 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6905 DraI TTT'AAA Cuts at: 4227 4244 4227 Size: 17 6923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2160 5947 809 Size: 507 844 3787 1802 Fragments arranged by size: 3787 1802 844 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2294 4387 5276 Size: 54 1425 50 275 18 464 2093 889 Cuts at: 5276 6905 8 Size: 1629 43 Fragments arranged by size: 2093 1629 1425 889 464 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4387 1537 Size: 275 2575 4090 Fragments arranged by size: 4090 2575 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 4870 1353 Size: 1032 2485 3423 Fragments arranged by size: 3423 2485 1032 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5527 5901 6047 387 Size: 1064 138 3938 374 146 1280 Fragments arranged by size: 3938 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3300 3423 3429 3880 3941 5003 5600 Size: 393 1668 123 6 451 61 1062 597 Cuts at: 5600 6387 6655 1239 Size: 787 268 1524 Fragments arranged by size: 1668 1524 1062 787 597 451 393 268 123 61 6 EcoO109I rG'GnC_Cy Cuts at: 3862 3862 Size: 6940 EcoRI G'AATT_C Cuts at: 4249 4627 4249 Size: 378 6562 EcoRV GAT'ATC Cuts at: 2599 2599 Size: 6940 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2735 2735 Size: 6940 FspI TGC'GCA Cuts at: 5446 5446 Size: 6940 HaeII r_GCGC'y Cuts at: 1678 6087 1678 Size: 4409 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2303 2335 2435 Size: 32 135 32 514 32 185 32 100 Cuts at: 2435 2454 2467 2486 2519 2551 3131 3163 5581 Size: 19 13 19 33 32 580 32 2418 Cuts at: 5581 5613 5655 5687 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2418 580 514 185 135 100 42 33 32 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 4284 4530 669 Size: 988 281 2346 246 3079 Fragments arranged by size: 3079 2346 988 281 246 HindIII A'AGCT_T Cuts at: 748 2683 748 Size: 1935 5005 HpaI GTT'AAC Cuts at: 4530 4530 Size: 6940 KpnI G_GTAC'C Cuts at: 3538 4265 3538 Size: 727 6213 MfeI C'AATT_G Cuts at: 2360 4539 2360 Size: 2179 4761 MluI A'CGCG_T Cuts at: 6816 6816 Size: 6940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3083 6054 6238 1305 Size: 1778 2971 184 2007 Fragments arranged by size: 2971 2007 1778 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2296 10 Size: 54 1425 343 464 4654 Fragments arranged by size: 4654 1425 464 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2426 2508 2627 2721 4174 4898 5257 Size: 648 1260 82 119 94 1453 724 359 Cuts at: 5257 5416 518 Size: 159 2042 Fragments arranged by size: 2042 1453 1260 724 648 359 159 119 94 82 MspA1I CmG'CkG Cuts at: 1606 4356 5033 6181 6426 1606 Size: 2750 677 1148 245 2120 Fragments arranged by size: 2750 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2214 513 Size: 552 939 210 5239 Fragments arranged by size: 5239 939 552 210 NdeI CA'TA_TG Cuts at: 387 2323 387 Size: 1936 5004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6940 NotI GC'GGCC_GC Cuts at: 4387 4387 Size: 6940 NspI r_CATG'y Cuts at: 1132 1229 2507 2632 4298 5843 1132 Size: 97 1278 125 1666 1545 2229 Fragments arranged by size: 2229 1666 1545 1278 125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6940 PciI A'CATG_T Cuts at: 5839 5839 Size: 6940 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6940 PfoI T'CCnGG_A Cuts at: 3316 3316 Size: 6940 PmeI GTTT'AAAC Cuts at: 4244 4244 Size: 6940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5009 5041 6522 6554 5009 Size: 32 1481 32 5395 Fragments arranged by size: 5395 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3862 3862 Size: 6940 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6940 PsiI TTA'TAA Cuts at: 4510 4510 Size: 6940 PstI C_TGCA'G Cuts at: 830 2022 3870 3984 4292 830 Size: 1192 1848 114 308 3478 Fragments arranged by size: 3478 1848 1192 308 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6940 SalI G'TCGA_C Cuts at: 1936 4282 1936 Size: 2346 4594 SapI GCTCTTCn'nnn_ Cuts at: 2385 2385 Size: 6940 SbfI CC_TGCA'GG Cuts at: 4292 4292 Size: 6940 ScaI AGT'ACT Cuts at: 4054 5188 6900 4054 Size: 1134 1712 4094 Fragments arranged by size: 4094 1712 1134 SfcI C'TryA_G Cuts at: 826 1045 2018 2562 3786 3866 3980 4288 5423 Size: 219 973 544 1224 80 114 308 1135 Cuts at: 5423 6104 6295 826 Size: 681 191 1471 Fragments arranged by size: 1471 1224 1135 973 681 544 308 219 191 114 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6940 SmaI CCC'GGG Cuts at: 1872 4267 1872 Size: 2395 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5044 5945 6207 820 Size: 197 926 3101 901 262 1553 Fragments arranged by size: 3101 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4731 493 Size: 4238 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6940 SphI G_CATG'C Cuts at: 1132 4298 1132 Size: 3166 3774 SspI AAT'ATT Cuts at: 5 52 3405 4864 5 Size: 47 3353 1459 2081 Fragments arranged by size: 3353 2081 1459 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6940 StyI C'CwwG_G Cuts at: 513 1065 2004 2214 2373 3265 4379 513 Size: 552 939 210 159 892 1114 3074 Fragments arranged by size: 3074 1114 939 892 552 210 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4967 4984 5137 5322 5767 325 Size: 1255 3387 17 153 185 445 1498 Fragments arranged by size: 3387 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 3416 4052 Size: 274 80 33 51 1443 296 1143 636 Cuts at: 4052 4234 5186 6898 96 Size: 182 952 1712 138 Fragments arranged by size: 1712 1443 1143 952 636 296 274 182 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2071 2103 2940 2972 3650 3682 6900 6932 2071 Size: 32 837 32 678 32 3218 32 2079 Fragments arranged by size: 3218 2079 837 678 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6940 XbaI T'CTAG_A Cuts at: 2242 4276 2242 Size: 2034 4906 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6940 XmaI C'CCGG_G Cuts at: 1870 4265 1870 Size: 2395 4545 XmnI GAAnn'nnTTC Cuts at: 5069 5069 Size: 6940 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4748 276 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI