(Circular) (Six-base) MAPSORT of: pFN21AB6775.seq Check: 7439 from: 1 to: 5059 pFN21AB6775 5059 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2869 278 Size: 53 83 186 936 1333 2468 Fragments arranged by size: 2468 1333 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2402 1937 Size: 465 4594 Acc65I G'GTAC_C Cuts at: 2233 2380 2233 Size: 147 4912 AclI AA'CG_TT Cuts at: 1563 3186 3559 1563 Size: 1623 373 3063 Fragments arranged by size: 3063 1623 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3122 4506 4774 1632 Size: 1490 1384 268 1917 Fragments arranged by size: 1917 1490 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4862 AflIII A'CryG_T Cuts at: 1203 3958 4935 1203 Size: 2755 977 1327 Fragments arranged by size: 2755 1327 977 AgeI A'CCGG_T Cuts at: 2081 3890 2081 Size: 1809 3250 AhdI GACnn_n'nnGTC Cuts at: 3788 3788 Size: 5059 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 1184 Size: 1032 4027 AlwNI CAG_nnn'CTG Cuts at: 2802 4374 2802 Size: 1572 3487 ApaLI G'TGCA_C Cuts at: 3116 4272 3116 Size: 1156 3903 ApoI r'AATT_y Cuts at: 1493 2072 2368 2594 2746 4855 1493 Size: 579 296 226 152 2109 1697 Fragments arranged by size: 2109 1697 579 296 226 152 AseI AT'TA_AT Cuts at: 160 1032 3613 160 Size: 872 2581 1606 Fragments arranged by size: 2581 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5059 AvaI C'yCGr_G Cuts at: 1870 1943 2384 1870 Size: 73 441 4545 Fragments arranged by size: 4545 441 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2217 2250 2217 Size: 33 5026 BamHI G'GATC_C Cuts at: 2389 2756 2389 Size: 367 4692 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2380 3835 618 Size: 325 217 627 446 147 1455 1842 Fragments arranged by size: 1842 1455 627 446 325 217 147 BanII G_rGCy'C Cuts at: 1391 2378 1391 Size: 987 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3250 3284 1903 Size: 34 1313 34 3678 Fragments arranged by size: 3678 1313 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2951 4161 2951 Size: 1210 3849 BclI T'GATC_A Cuts at: 1548 1548 Size: 5059 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3670 136 Size: 107 122 71 631 605 1998 1525 Fragments arranged by size: 1998 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5054 5054 Size: 5059 BlpI GC'TnA_GC Cuts at: 2476 2476 Size: 5059 Bme1580I G_kGCm'C Cuts at: 1790 3120 4276 1790 Size: 1330 1156 2573 Fragments arranged by size: 2573 1330 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5059 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2790 3748 442 Size: 416 53 83 399 24 1373 958 1753 Fragments arranged by size: 1753 1373 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5059 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 3719 1239 Size: 986 1494 2579 Fragments arranged by size: 2579 1494 986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3184 4049 4347 3184 Size: 865 298 3896 Fragments arranged by size: 3896 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3722 882 Size: 617 2223 2219 Fragments arranged by size: 2223 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2850 493 Size: 711 1646 2702 Fragments arranged by size: 2702 1646 711 BsaBI GATnn'nnATC Cuts at: 5053 5053 Size: 5059 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2866 3248 3883 275 Size: 53 83 186 936 1333 382 635 1451 Fragments arranged by size: 1451 1333 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2081 3492 3890 4164 4311 4722 2081 Size: 1411 398 274 147 411 2418 Fragments arranged by size: 2418 1411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4834 Fragments arranged by size: 4834 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2060 2078 2081 1186 Size: 817 57 18 3 4164 Fragments arranged by size: 4164 817 57 18 3 BseYI C'CCAG_C Cuts at: 4262 4758 4262 Size: 496 4563 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4486 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2509 3270 4298 1540 Size: 275 186 508 761 1028 2301 Fragments arranged by size: 2301 1028 761 508 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2133 3120 3205 4276 2100 Size: 33 987 85 1071 2883 Fragments arranged by size: 2883 1071 987 85 33 BsmI GAATG_Cn' Cuts at: 2570 2663 2570 Size: 93 4966 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2133 2378 3120 3205 4276 1391 Size: 399 310 33 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 245 85 33 BspEI T'CCGG_A Cuts at: 4722 4722 Size: 5059 BspHI T'CATG_A Cuts at: 2948 4678 2948 Size: 1730 3329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 844 Size: 1570 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2344 2946 1394 Size: 429 521 602 3507 Fragments arranged by size: 3507 602 521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3554 3728 65 Size: 1183 2306 174 1396 Fragments arranged by size: 2306 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 2351 3703 3890 1700 Size: 110 271 270 1352 187 2869 Fragments arranged by size: 2869 1352 271 270 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5059 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5059 BssSI C'ACGA_G Cuts at: 3119 4131 3119 Size: 1012 4047 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5059 BstBI TT'CG_AA Cuts at: 3863 3863 Size: 5059 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5059 BstYI r'GATC_y Cuts at: 1972 2389 2756 3141 3158 4610 4696 4708 5054 Size: 417 367 385 17 1452 86 12 346 Cuts at: 5054 1972 Size: 1977 Fragments arranged by size: 1977 1452 417 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 487 Size: 683 56 1106 3214 Fragments arranged by size: 3214 1106 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2575 3369 3389 2188 Size: 387 794 20 3858 Fragments arranged by size: 3858 794 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5059 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5024 DraI TTT'AAA Cuts at: 2363 2363 Size: 5059 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4066 809 Size: 507 2750 1802 Fragments arranged by size: 2750 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2506 3395 5024 Size: 54 1425 50 275 18 676 889 1629 Cuts at: 5024 8 Size: 43 Fragments arranged by size: 1629 1425 889 676 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2506 1537 Size: 275 694 4090 Fragments arranged by size: 4090 694 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2989 1353 Size: 1636 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3646 4020 4166 387 Size: 1064 138 2057 374 146 1280 Fragments arranged by size: 2057 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 3122 3719 4506 4774 1239 Size: 393 593 897 597 787 268 1524 Fragments arranged by size: 1524 897 787 597 593 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2182 2182 Size: 5059 EcoRI G'AATT_C Cuts at: 2368 2746 2368 Size: 378 4681 FspI TGC'GCA Cuts at: 3565 3565 Size: 5059 HaeII r_GCGC'y Cuts at: 1678 4206 1678 Size: 2528 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3700 3732 3774 3806 1373 Size: 32 135 32 2128 32 42 32 2626 Fragments arranged by size: 2626 2128 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2403 2649 669 Size: 988 281 337 128 246 3079 Fragments arranged by size: 3079 988 337 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 5059 HpaI GTT'AAC Cuts at: 2649 2649 Size: 5059 KpnI G_GTAC'C Cuts at: 2237 2384 2237 Size: 147 4912 MfeI C'AATT_G Cuts at: 2658 2658 Size: 5059 MluI A'CGCG_T Cuts at: 4935 4935 Size: 5059 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4173 4357 1305 Size: 2868 184 2007 Fragments arranged by size: 2868 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3237 Fragments arranged by size: 3237 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3376 3535 518 Size: 648 1851 359 159 2042 Fragments arranged by size: 2042 1851 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2475 3152 4300 4545 1606 Size: 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 677 245 NaeI GCC'GGC Cuts at: 2353 2353 Size: 5059 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4507 NdeI CA'TA_TG Cuts at: 387 387 Size: 5059 NgoMIV G'CCGG_C Cuts at: 2351 2351 Size: 5059 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5059 NotI GC'GGCC_GC Cuts at: 2506 2506 Size: 5059 NspI r_CATG'y Cuts at: 1132 1229 2417 3962 1132 Size: 97 1188 1545 2229 Fragments arranged by size: 2229 1545 1188 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5059 PciI A'CATG_T Cuts at: 3958 3958 Size: 5059 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5059 PmeI GTTT'AAAC Cuts at: 2363 2363 Size: 5059 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3128 3160 4641 4673 3128 Size: 32 1481 32 3514 Fragments arranged by size: 3514 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5059 PsiI TTA'TAA Cuts at: 2629 2629 Size: 5059 PstI C_TGCA'G Cuts at: 830 2055 2411 830 Size: 1225 356 3478 Fragments arranged by size: 3478 1225 356 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5059 SalI G'TCGA_C Cuts at: 1936 2401 1936 Size: 465 4594 SbfI CC_TGCA'GG Cuts at: 2411 2411 Size: 5059 ScaI AGT'ACT Cuts at: 3307 5019 3307 Size: 1712 3347 SfcI C'TryA_G Cuts at: 826 1045 2051 2407 3542 4223 4414 826 Size: 219 1006 356 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 356 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5059 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5059 SmaI CCC'GGG Cuts at: 1872 2386 1872 Size: 514 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3163 4064 4326 820 Size: 197 926 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2850 493 Size: 2357 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5059 SphI G_CATG'C Cuts at: 1132 2417 1132 Size: 1285 3774 SspI AAT'ATT Cuts at: 5 52 2983 5 Size: 47 2931 2081 Fragments arranged by size: 2931 2081 47 StuI AGG'CCT Cuts at: 1517 2177 1517 Size: 660 4399 StyI C'CwwG_G Cuts at: 513 1065 2498 513 Size: 552 1433 3074 Fragments arranged by size: 3074 1433 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3103 3256 3441 3886 325 Size: 1255 1506 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3305 5017 96 Size: 274 80 33 51 1443 1328 1712 138 Fragments arranged by size: 1712 1443 1328 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5019 5051 5019 Size: 32 5027 Tth111I GACn'n_nGTC Cuts at: 1316 2318 1316 Size: 1002 4057 XbaI T'CTAG_A Cuts at: 2395 2395 Size: 5059 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5059 XmaI C'CCGG_G Cuts at: 1870 2384 1870 Size: 514 4545 XmnI GAAnn'nnTTC Cuts at: 2177 3188 2177 Size: 1011 4048 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2867 276 Size: 53 83 186 936 1333 2468 Fragments arranged by size: 2468 1333 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI