(Circular) (Six-base) MAPSORT of: pFN21AB7664.seq Check: 4606 from: 1 to: 5893 pFN21AB7664 5893 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2857 3236 1937 Size: 920 379 4594 Fragments arranged by size: 4594 920 379 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 5340 5608 1632 Size: 2324 1384 268 1917 Fragments arranged by size: 2324 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 2151 1166 Size: 985 4908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 2518 3636 5208 2518 Size: 1118 1572 3203 Fragments arranged by size: 3203 1572 1118 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 3080 3202 3428 3580 5689 1493 Size: 1587 122 226 152 2109 1697 Fragments arranged by size: 2109 1697 1587 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2191 2214 3218 1870 Size: 73 248 23 1004 4545 Fragments arranged by size: 4545 1004 248 73 23 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2656 3214 4669 618 Size: 325 217 627 869 558 1455 1842 Fragments arranged by size: 1842 1455 869 627 558 325 217 BanII G_rGCy'C Cuts at: 1391 2215 2751 3212 1391 Size: 824 536 461 4072 Fragments arranged by size: 4072 824 536 461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2053 928 Size: 971 154 4768 Fragments arranged by size: 4768 971 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2774 3785 4995 2774 Size: 1011 1210 3672 Fragments arranged by size: 3672 1210 1011 BclI T'GATC_A Cuts at: 1548 2887 1548 Size: 1339 4554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4504 136 Size: 107 122 71 631 605 337 2495 1525 Fragments arranged by size: 2495 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 2661 2698 3954 5110 1790 Size: 871 37 1256 1156 2573 Fragments arranged by size: 2573 1256 1156 871 37 BmgBI CAC'GTC Cuts at: 1318 2065 1318 Size: 747 5146 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2861 3624 4582 Size: 416 53 83 399 24 1444 763 958 Cuts at: 4582 442 Size: 1753 Fragments arranged by size: 1753 1444 958 763 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4553 1239 Size: 3314 2579 Bpu10I CC'TnA_GC Cuts at: 2526 3001 2526 Size: 475 5418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4018 4883 5181 2723 Size: 1295 865 298 3435 Fragments arranged by size: 3435 1295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2762 3684 493 Size: 711 1558 922 2702 Fragments arranged by size: 2702 1558 922 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2782 3700 4082 4717 Size: 53 83 186 936 1249 918 382 635 Cuts at: 4717 275 Size: 1451 Fragments arranged by size: 1451 1249 936 918 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2140 2150 4326 4724 4998 5145 5556 2140 Size: 10 2176 398 274 147 411 2477 Fragments arranged by size: 2477 2176 411 398 274 147 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5668 Fragments arranged by size: 5668 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2232 2268 3179 3182 3185 1186 Size: 1046 36 911 3 3 3894 Fragments arranged by size: 3894 1046 911 36 3 3 BseYI C'CCAG_C Cuts at: 5096 5592 5096 Size: 496 5397 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 BsmBI CGTCTCn'nnnn_ Cuts at: 2108 2169 2789 2108 Size: 61 620 5212 Fragments arranged by size: 5212 620 61 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2215 2661 2698 2751 3212 3954 4039 Size: 399 425 446 37 53 461 742 85 Cuts at: 4039 5110 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 461 446 425 399 85 53 37 BspEI T'CCGG_A Cuts at: 2140 5556 2140 Size: 3416 2477 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 3248 844 Size: 1682 722 3489 Fragments arranged by size: 3489 1682 722 BsrBI CCG'CTC Cuts at: 1394 1823 2190 2539 3780 1394 Size: 429 367 349 1241 3507 Fragments arranged by size: 3507 1241 429 367 349 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 3013 4388 4562 65 Size: 1183 1620 145 1375 174 1396 Fragments arranged by size: 1620 1396 1375 1183 174 145 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2074 2182 2226 4537 4724 1700 Size: 110 230 34 108 44 2311 187 2869 Fragments arranged by size: 2869 2311 230 187 110 108 44 34 BsrGI T'GTAC_A Cuts at: 96 2823 96 Size: 2727 3166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2153 Size: 5893 BstYI r'GATC_y Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888 Size: 1251 367 385 17 1452 86 12 346 Cuts at: 5888 1972 Size: 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2257 2257 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 2200 513 Size: 552 1135 4206 Fragments arranged by size: 4206 1135 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2555 487 Size: 683 56 1329 3825 Fragments arranged by size: 3825 1329 683 56 BtsI GCAGTG_nn' Cuts at: 2608 3409 4203 4223 2608 Size: 801 794 20 4278 Fragments arranged by size: 4278 801 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 2797 3197 2797 Size: 400 5493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 3340 4229 Size: 54 1425 50 275 18 1135 375 889 Cuts at: 4229 5858 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1135 889 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2529 2965 3823 1353 Size: 659 517 436 858 3423 Fragments arranged by size: 3423 858 659 517 436 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2157 4480 4854 5000 387 Size: 1064 138 568 2323 374 146 1280 Fragments arranged by size: 2323 1280 1064 568 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3956 4553 5340 5608 1239 Size: 393 2324 597 787 268 1524 Fragments arranged by size: 2324 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 2984 3016 3090 Size: 32 135 32 1307 32 73 32 74 Cuts at: 3090 3122 4534 4566 4608 4640 1373 Size: 32 1412 32 42 32 2626 Fragments arranged by size: 2626 1412 1307 135 74 73 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2858 3237 3483 669 Size: 988 281 920 379 246 3079 Fragments arranged by size: 3079 988 920 379 281 246 HindIII A'AGCT_T Cuts at: 748 2677 748 Size: 1929 3964 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 2883 3492 2883 Size: 609 5284 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 5007 5191 1305 Size: 1800 1902 184 2007 Fragments arranged by size: 2007 1902 1800 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 3851 4210 4369 518 Size: 648 985 1700 359 159 2042 Fragments arranged by size: 2042 1700 985 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 3309 3986 5134 5379 1606 Size: 596 1107 677 1148 245 2120 Fragments arranged by size: 2120 1148 1107 677 596 245 NaeI GCC'GGC Cuts at: 2042 2076 2184 2042 Size: 34 108 5751 Fragments arranged by size: 5751 108 34 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NgoMIV G'CCGG_C Cuts at: 2040 2074 2182 2040 Size: 34 108 5751 Fragments arranged by size: 5751 108 34 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2915 3251 4796 1132 Size: 97 1686 336 1545 2229 Fragments arranged by size: 2229 1686 1545 336 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 2550 3463 2550 Size: 913 4980 PstI C_TGCA'G Cuts at: 830 2523 3245 830 Size: 1693 722 3478 Fragments arranged by size: 3478 1693 722 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacII CC_GC'GG Cuts at: 2203 2203 Size: 5893 SalI G'TCGA_C Cuts at: 1936 2856 3235 1936 Size: 920 379 4594 Fragments arranged by size: 4594 920 379 SapI GCTCTTCn'nnn_ Cuts at: 2012 2529 2012 Size: 517 5376 SbfI CC_TGCA'GG Cuts at: 2523 3245 2523 Size: 722 5171 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2519 3241 4376 5057 5248 826 Size: 219 1474 722 1135 681 191 1471 Fragments arranged by size: 1474 1471 1135 722 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 3997 4898 5160 820 Size: 197 926 759 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 759 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 2915 3251 1132 Size: 1783 336 3774 Fragments arranged by size: 3774 1783 336 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 2255 2473 1517 Size: 738 218 4937 Fragments arranged by size: 4937 738 218 StyI C'CwwG_G Cuts at: 513 1065 2995 3332 513 Size: 552 1930 337 3074 Fragments arranged by size: 3074 1930 552 337 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 3920 3937 4090 4275 4720 325 Size: 1255 671 1669 17 153 185 445 1498 Fragments arranged by size: 1669 1498 1255 671 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3073 4139 Size: 274 80 33 51 1443 846 250 1066 Cuts at: 4139 5851 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1066 846 274 250 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI