(Circular) (Six-base) MAPSORT of: pFN21AB3929.seq Check: 2039 from: 1 to: 5479 pFN21AB3929 5479 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 3542 4926 5194 1632 Size: 1040 870 1384 268 1917 Fragments arranged by size: 1917 1384 1040 870 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 4378 5355 1203 Size: 3175 977 1327 Fragments arranged by size: 3175 1327 977 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5479 AlwNI CAG_nnn'CTG Cuts at: 2528 3222 4794 2528 Size: 694 1572 3213 Fragments arranged by size: 3213 1572 694 ApaI G_GGCC'C Cuts at: 2109 2109 Size: 5479 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2788 3014 3166 5275 1493 Size: 1295 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2804 1870 Size: 73 145 716 4545 Fragments arranged by size: 4545 716 145 73 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2411 2800 4255 618 Size: 325 217 627 273 351 389 1455 1842 Fragments arranged by size: 1842 1455 627 389 351 325 273 217 BanII G_rGCy'C Cuts at: 1391 2109 2247 2798 1391 Size: 718 138 551 4072 Fragments arranged by size: 4072 718 551 138 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 5479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4508 BbvCI CC'TCA_GC Cuts at: 2163 2163 Size: 5479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3670 3704 1903 Size: 34 1733 34 3678 Fragments arranged by size: 3678 1733 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 4090 136 Size: 107 122 71 631 605 397 2021 1525 Fragments arranged by size: 2021 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2896 2896 Size: 5479 Bme1580I G_kGCm'C Cuts at: 1790 2109 3540 4696 1790 Size: 319 1431 1156 2573 Fragments arranged by size: 2573 1431 1156 319 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2651 3210 4168 Size: 416 53 83 399 24 1234 559 958 Cuts at: 4168 442 Size: 1753 Fragments arranged by size: 1753 1234 958 559 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 4139 1239 Size: 1049 1851 2579 Fragments arranged by size: 2579 1851 1049 Bpu10I CC'TnA_GC Cuts at: 2163 2163 Size: 5479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 4469 4767 3604 Size: 865 298 4316 Fragments arranged by size: 4316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2085 2384 4142 882 Size: 617 586 299 1758 2219 Fragments arranged by size: 2219 1758 617 586 299 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2085 3286 3668 Size: 53 83 186 936 528 24 1201 382 Cuts at: 3668 4303 275 Size: 635 1451 Fragments arranged by size: 1451 1201 936 635 528 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 3912 4310 4584 4731 5142 3912 Size: 398 274 147 411 4249 Fragments arranged by size: 4249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5254 Fragments arranged by size: 5254 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2274 2702 1186 Size: 1069 19 428 3963 Fragments arranged by size: 3963 1069 428 19 BseYI C'CCAG_C Cuts at: 2424 4682 5178 2424 Size: 2258 496 2725 Fragments arranged by size: 2725 2258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4906 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 2175 2929 3690 4718 1540 Size: 275 186 68 106 754 761 1028 2301 Fragments arranged by size: 2301 1028 761 754 275 186 106 68 BsiHKAI G_wGCw'C Cuts at: 2247 3540 3625 4696 2247 Size: 1293 85 1071 3030 Fragments arranged by size: 3030 1293 1071 85 BsmI GAATG_Cn' Cuts at: 2990 3083 2990 Size: 93 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2247 2798 3540 3625 4696 1391 Size: 399 319 138 551 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 399 319 138 85 BspEI T'CCGG_A Cuts at: 5142 5142 Size: 5479 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2834 844 Size: 1334 656 3489 Fragments arranged by size: 3489 1334 656 BsrBI CCG'CTC Cuts at: 1394 1823 3366 1394 Size: 429 1543 3507 Fragments arranged by size: 3507 1543 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3974 4148 65 Size: 1183 2726 174 1396 Fragments arranged by size: 2726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 2709 4123 4310 1700 Size: 110 258 641 1414 187 2869 Fragments arranged by size: 2869 1414 641 258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5479 BssSI C'ACGA_G Cuts at: 3539 4551 3539 Size: 1012 4467 BstAPI GCAn_nnn'nTGC Cuts at: 794 2137 794 Size: 1343 4136 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 2169 2634 513 Size: 552 1053 51 465 3358 Fragments arranged by size: 3358 1053 552 465 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2995 3789 3809 2995 Size: 794 20 4665 Fragments arranged by size: 4665 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2706 2741 529 Size: 35 2142 35 3267 Fragments arranged by size: 3267 2142 35 35 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2646 4486 809 Size: 507 1330 1840 1802 Fragments arranged by size: 1840 1802 1330 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2172 2926 Size: 54 1425 50 275 18 236 106 754 Cuts at: 2926 3815 5444 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 754 275 236 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2066 2172 2926 1537 Size: 275 254 106 754 4090 Fragments arranged by size: 4090 754 275 254 106 EarI CTCTTCn'nnn_ Cuts at: 1353 2239 3409 1353 Size: 886 1170 3423 Fragments arranged by size: 3423 1170 886 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 4440 4586 387 Size: 1064 138 2477 374 146 1280 Fragments arranged by size: 2477 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2672 3542 4139 4926 5194 1239 Size: 393 656 384 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 656 597 393 384 268 EcoO109I rG'GnC_Cy Cuts at: 2149 2190 2149 Size: 41 5438 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FseI GG_CCGG'CC Cuts at: 2072 2072 Size: 5479 FspI TGC'GCA Cuts at: 3985 3985 Size: 5479 HaeII r_GCGC'y Cuts at: 1678 2064 4626 1678 Size: 386 2562 2531 Fragments arranged by size: 2562 2531 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2615 2647 4120 4152 4194 Size: 32 135 32 1043 32 1473 32 42 Cuts at: 4194 4226 1373 Size: 32 2626 Fragments arranged by size: 2626 1473 1043 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2743 2823 3069 669 Size: 988 281 805 80 246 3079 Fragments arranged by size: 3079 988 805 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KasI G'GCGC_C Cuts at: 2060 2060 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4593 4777 1305 Size: 3288 184 2007 Fragments arranged by size: 3288 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3657 Fragments arranged by size: 3657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3437 3796 3955 518 Size: 648 2271 359 159 2042 Fragments arranged by size: 2271 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2895 3572 4720 4965 1606 Size: 514 775 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 514 245 NaeI GCC'GGC Cuts at: 2070 2070 Size: 5479 NarI GG'CG_CC Cuts at: 2061 2061 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 2634 513 Size: 552 1569 3358 Fragments arranged by size: 3358 1569 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NgoMIV G'CCGG_C Cuts at: 2068 2068 Size: 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5479 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2837 4382 1132 Size: 97 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 97 PasI CC'CwG_GG Cuts at: 1348 2665 1348 Size: 1317 4162 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5479 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 5479 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2190 2149 Size: 41 5438 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PspOMI G'GGCC_C Cuts at: 2105 2105 Size: 5479 PstI C_TGCA'G Cuts at: 830 2831 830 Size: 2001 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5479 SacII CC_GC'GG Cuts at: 2121 2121 Size: 5479 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 2135 2560 3727 5439 2135 Size: 425 1167 1712 2175 Fragments arranged by size: 2175 1712 1167 425 SfcI C'TryA_G Cuts at: 826 1045 2827 3962 4643 4834 826 Size: 219 1782 1135 681 191 1471 Fragments arranged by size: 1782 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2062 2062 Size: 5479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2806 1872 Size: 934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 4484 4746 820 Size: 197 926 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5479 StyI C'CwwG_G Cuts at: 513 1065 2075 2634 2918 513 Size: 552 1010 559 284 3074 Fragments arranged by size: 3074 1010 559 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2511 3506 3523 3676 3861 4306 325 Size: 1255 931 995 17 153 185 445 1498 Fragments arranged by size: 1498 1255 995 931 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 2456 2558 Size: 274 80 33 51 1443 156 323 102 Cuts at: 2558 3725 5437 96 Size: 1167 1712 138 Fragments arranged by size: 1712 1443 1167 323 274 156 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 5447 Tth111I GACn'n_nGTC Cuts at: 1316 2528 2643 1316 Size: 1212 115 4152 Fragments arranged by size: 4152 1212 115 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2191 Size: 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2804 1870 Size: 934 4545 XmnI GAAnn'nnTTC Cuts at: 3608 3608 Size: 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI