(Circular) (Six-base) MAPSORT of: pFN21AB7601.seq Check: 631 from: 1 to: 5581 pFN21AB7601 5581 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2527 2541 2564 2642 2527 Size: 14 23 78 5466 Fragments arranged by size: 5466 78 23 14 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3391 278 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 2924 1937 Size: 346 641 4594 Fragments arranged by size: 4594 641 346 Acc65I G'GTAC_C Cuts at: 2902 2902 Size: 5581 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3644 5028 5296 1632 Size: 2012 1384 268 1917 Fragments arranged by size: 2012 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5384 AflIII A'CryG_T Cuts at: 1203 4480 5457 1203 Size: 3277 977 1327 Fragments arranged by size: 3277 1327 977 AgeI A'CCGG_T Cuts at: 2799 4412 2799 Size: 1613 3968 AhdI GACnn_n'nnGTC Cuts at: 4310 4310 Size: 5581 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2228 1184 Size: 1044 4537 AlwNI CAG_nnn'CTG Cuts at: 2667 3324 4896 2667 Size: 657 1572 3352 Fragments arranged by size: 3352 1572 657 ApaI G_GGCC'C Cuts at: 2393 2767 2879 2393 Size: 374 112 5095 Fragments arranged by size: 5095 374 112 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2890 3116 3268 5377 1493 Size: 1397 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 226 152 AseI AT'TA_AT Cuts at: 160 1032 4135 160 Size: 872 3103 1606 Fragments arranged by size: 3103 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2289 2442 2449 2872 2906 1870 Size: 73 190 156 153 7 423 34 4545 Fragments arranged by size: 4545 423 190 156 153 73 34 7 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2119 2511 2591 2802 Size: 325 217 627 303 29 392 80 211 Cuts at: 2802 2902 4357 618 Size: 100 1455 1842 Fragments arranged by size: 1842 1455 627 392 325 303 217 211 100 80 29 BanII G_rGCy'C Cuts at: 1391 2393 2410 2449 2767 2879 2900 1391 Size: 1002 17 39 318 112 21 4072 Fragments arranged by size: 4072 1002 318 112 39 21 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2542 928 Size: 971 643 3967 Fragments arranged by size: 3967 971 643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3772 3806 1903 Size: 34 50 34 1751 34 3678 Fragments arranged by size: 3678 1751 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 4683 3473 Size: 1210 4371 BclI T'GATC_A Cuts at: 1548 1548 Size: 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2300 2504 4192 Size: 107 122 71 631 605 628 204 1688 Cuts at: 4192 136 Size: 1525 Fragments arranged by size: 1688 1525 631 628 605 204 122 107 71 BglII A'GATC_T Cuts at: 2640 5576 2640 Size: 2936 2645 BlpI GC'TnA_GC Cuts at: 2430 2998 2430 Size: 568 5013 Bme1580I G_kGCm'C Cuts at: 1790 2124 2160 2393 2596 2767 2879 3642 4798 Size: 334 36 233 203 171 112 763 1156 Cuts at: 4798 1790 Size: 2573 Fragments arranged by size: 2573 1156 763 334 233 203 171 112 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 4270 442 Size: 416 53 83 399 24 1895 958 1753 Fragments arranged by size: 1895 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2087 2644 4241 1239 Size: 781 67 557 1597 2579 Fragments arranged by size: 2579 1597 781 557 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 3706 4571 4869 2582 Size: 1124 865 298 3294 Fragments arranged by size: 3294 1124 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4244 882 Size: 617 2745 2219 Fragments arranged by size: 2745 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3372 493 Size: 711 2168 2702 Fragments arranged by size: 2702 2168 711 BsaBI GATnn'nnATC Cuts at: 2606 5575 2606 Size: 2969 2612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 2787 3388 3770 Size: 53 83 186 936 729 525 601 382 Cuts at: 3770 4405 275 Size: 635 1451 Fragments arranged by size: 1451 936 729 635 601 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2410 2799 4014 4412 4686 4833 5244 2410 Size: 389 1215 398 274 147 411 2747 Fragments arranged by size: 2747 1215 411 398 389 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5356 Fragments arranged by size: 5356 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2179 2870 1186 Size: 931 62 691 3897 Fragments arranged by size: 3897 931 691 62 BseYI C'CCAG_C Cuts at: 2172 4784 5280 2172 Size: 2612 496 2473 Fragments arranged by size: 2612 2473 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 2614 2797 1163 Size: 573 771 107 183 3947 Fragments arranged by size: 3947 771 573 183 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 2749 3031 3792 4820 1540 Size: 275 186 195 553 282 761 1028 2301 Fragments arranged by size: 2301 1028 761 553 282 275 195 186 BsiHKAI G_wGCw'C Cuts at: 3642 3727 4798 3642 Size: 85 1071 4425 Fragments arranged by size: 4425 1071 85 BsmI GAATG_Cn' Cuts at: 3092 3185 3092 Size: 93 5488 BsmBI CGTCTCn'nnnn_ Cuts at: 2063 2063 Size: 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2160 2393 2410 2449 2596 2767 Size: 399 334 36 233 17 39 147 171 Cuts at: 2767 2879 2900 3642 3727 4798 1391 Size: 112 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 233 171 147 112 85 39 36 21 17 BspEI T'CCGG_A Cuts at: 2410 5244 2410 Size: 2834 2747 BspHI T'CATG_A Cuts at: 3470 5200 3470 Size: 1730 3851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2527 2541 2564 2642 2936 844 Size: 1508 175 14 23 78 294 3489 Fragments arranged by size: 3489 1508 294 175 78 23 14 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2434 2751 3468 1394 Size: 429 364 247 317 717 3507 Fragments arranged by size: 3507 717 429 364 317 247 BsrDI GCAATG_nn' Cuts at: 65 1248 4076 4250 65 Size: 1183 2828 174 1396 Fragments arranged by size: 2828 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 2397 2514 2799 2805 4225 4412 Size: 110 248 339 117 285 6 1420 187 Cuts at: 4412 1700 Size: 2869 Fragments arranged by size: 2869 1420 339 285 248 187 117 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5581 BssSI C'ACGA_G Cuts at: 3641 4653 3641 Size: 1012 4569 BstAPI GCAn_nnn'nTGC Cuts at: 794 2565 794 Size: 1771 3810 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 2162 1184 Size: 978 4603 BstYI r'GATC_y Cuts at: 1972 2640 2911 3278 3663 3680 5132 5218 5230 Size: 668 271 367 385 17 1452 86 12 Cuts at: 5230 5576 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 668 385 367 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2225 513 Size: 552 1160 3869 Fragments arranged by size: 3869 1160 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4842 Fragments arranged by size: 4842 683 56 BtsI GCAGTG_nn' Cuts at: 2351 2470 2524 2725 3097 3891 3911 2351 Size: 119 54 201 372 794 20 4021 Fragments arranged by size: 4021 794 372 201 119 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2626 2661 529 Size: 35 2062 35 3449 Fragments arranged by size: 3449 2062 35 35 DraI TTT'AAA Cuts at: 2885 2885 Size: 5581 DraIII CAC_nnn'GTG Cuts at: 2373 2373 Size: 5581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2496 2505 Size: 54 1425 50 275 18 363 303 9 Cuts at: 2505 3028 3917 5546 8 Size: 523 889 1629 43 Fragments arranged by size: 1629 1425 889 523 363 303 275 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2193 3028 1537 Size: 275 381 835 4090 Fragments arranged by size: 4090 835 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3511 1353 Size: 2158 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4168 4542 4688 387 Size: 1064 138 2579 374 146 1280 Fragments arranged by size: 2579 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2087 2644 3644 4241 5028 5296 Size: 393 388 67 557 1000 597 787 268 Cuts at: 5296 1239 Size: 1524 Fragments arranged by size: 1524 1000 787 597 557 393 388 268 67 EcoNI CCTnn'n_nnAGG Cuts at: 2659 2659 Size: 5581 EcoO109I rG'GnC_Cy Cuts at: 2047 2146 2247 2664 2736 2876 2047 Size: 99 101 417 72 140 4752 Fragments arranged by size: 4752 417 140 101 99 72 EcoRI G'AATT_C Cuts at: 2890 3268 2890 Size: 378 5203 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 2812 4728 1678 Size: 1134 1916 2531 Fragments arranged by size: 2531 1916 1134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 4222 4254 4296 Size: 32 135 32 602 32 2016 32 42 Cuts at: 4296 4328 1373 Size: 32 2626 Fragments arranged by size: 2626 2016 602 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2284 2925 3171 669 Size: 988 281 346 641 246 3079 Fragments arranged by size: 3079 988 641 346 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KpnI G_GTAC'C Cuts at: 2906 2906 Size: 5581 MfeI C'AATT_G Cuts at: 3180 3180 Size: 5581 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2778 4695 4879 1305 Size: 1473 1917 184 2007 Fragments arranged by size: 2007 1917 1473 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2507 10 Size: 54 1425 343 675 3084 Fragments arranged by size: 3084 1425 675 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3539 3898 4057 518 Size: 648 2373 359 159 2042 Fragments arranged by size: 2373 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2521 2997 3674 4822 5067 1606 Size: 915 476 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 677 476 245 NaeI GCC'GGC Cuts at: 2807 2807 Size: 5581 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5029 NdeI CA'TA_TG Cuts at: 387 387 Size: 5581 NgoMIV G'CCGG_C Cuts at: 2805 2805 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 3028 3028 Size: 5581 NspI r_CATG'y Cuts at: 1132 1229 2836 2939 4484 1132 Size: 97 1607 103 1545 2229 Fragments arranged by size: 2229 1607 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 2178 1348 Size: 830 4751 PciI A'CATG_T Cuts at: 4480 4480 Size: 5581 PflMI CCAn_nnn'nTGG Cuts at: 1108 2373 2494 2667 1108 Size: 1265 121 173 4022 Fragments arranged by size: 4022 1265 173 121 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3650 3682 5163 5195 3650 Size: 32 1481 32 4036 Fragments arranged by size: 4036 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2047 Size: 5581 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5581 PsiI TTA'TAA Cuts at: 3151 3151 Size: 5581 PspOMI G'GGCC_C Cuts at: 2389 2763 2875 2389 Size: 374 112 5095 Fragments arranged by size: 5095 374 112 PstI C_TGCA'G Cuts at: 830 2538 2679 2732 2933 830 Size: 1708 141 53 201 3478 Fragments arranged by size: 3478 1708 201 141 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 PvuII CAG'CTG Cuts at: 2521 2521 Size: 5581 SalI G'TCGA_C Cuts at: 1936 2282 2923 1936 Size: 346 641 4594 Fragments arranged by size: 4594 641 346 SanDI GG'GwC_CC Cuts at: 2047 2047 Size: 5581 SbfI CC_TGCA'GG Cuts at: 2538 2933 2538 Size: 395 5186 ScaI AGT'ACT Cuts at: 2564 3829 5541 2564 Size: 1265 1712 2604 Fragments arranged by size: 2604 1712 1265 SfcI C'TryA_G Cuts at: 826 1045 2534 2675 2728 2929 4064 4745 4936 Size: 219 1489 141 53 201 1135 681 191 Cuts at: 4936 826 Size: 1471 Fragments arranged by size: 1489 1471 1135 681 219 201 191 141 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2300 2504 2300 Size: 204 5377 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2451 2874 2908 1872 Size: 579 423 34 4545 Fragments arranged by size: 4545 579 423 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3685 4586 4848 820 Size: 197 926 654 1088 901 262 1553 Fragments arranged by size: 1553 1088 926 901 654 262 197 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SrfI GCCC'GGGC Cuts at: 2874 2874 Size: 5581 SspI AAT'ATT Cuts at: 5 52 3505 5 Size: 47 3453 2081 Fragments arranged by size: 3453 2081 47 StuI AGG'CCT Cuts at: 1517 2654 1517 Size: 1137 4444 StyI C'CwwG_G Cuts at: 513 1065 2265 2385 3020 513 Size: 552 1200 120 635 3074 Fragments arranged by size: 3074 1200 635 552 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2579 2708 3608 3625 3778 3963 4408 Size: 1255 999 129 900 17 153 185 445 Cuts at: 4408 325 Size: 1498 Fragments arranged by size: 1498 1255 999 900 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 2577 3827 Size: 274 80 33 51 1443 585 15 1250 Cuts at: 3827 5539 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1250 585 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5541 5573 5541 Size: 32 5549 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5581 XbaI T'CTAG_A Cuts at: 2917 2917 Size: 5581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5581 XmaI C'CCGG_G Cuts at: 1870 2449 2872 2906 1870 Size: 579 423 34 4545 Fragments arranged by size: 4545 579 423 34 XmnI GAAnn'nnTTC Cuts at: 3710 3710 Size: 5581 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3389 276 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SwaI XcmI