Restriction map for
pFN21AB7320
(Circular) (Six-base) MAPSORT of: pFN21AB7320.seq Check: 5894 from: 1 to: 8569
pFN21AB7320 8569 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6379 278
Size: 53 83 186 936 4843 2468
Fragments arranged by size:
4843 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3295 5496 5912 1937
Size: 1358 2201 416 4594
Fragments arranged by size:
4594 2201 1358 416
Acc65I G'GTAC_C
Cuts at: 5890 5890
Size: 8569
AclI AA'CG_TT
Cuts at: 1563 4590 6696 7069 1563
Size: 3027 2106 373 3063
Fragments arranged by size:
3063 3027 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3410 4341 5538 6632 8016 8284 1632
Size: 1778 931 1197 1094 1384 268 1917
Fragments arranged by size:
1917 1778 1384 1197 1094 931 268
AfeI AGC'GCT
Cuts at: 2324 2324
Size: 8569
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8372
AflIII A'CryG_T
Cuts at: 1203 7468 8445 1203
Size: 6265 977 1327
Fragments arranged by size:
6265 1327 977
AgeI A'CCGG_T
Cuts at: 7400 7400
Size: 8569
AhdI GACnn_n'nnGTC
Cuts at: 7298 7298
Size: 8569
AleI CACnn'nnGTG
Cuts at: 1166 2601 1166
Size: 1435 7134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 2267
Size: 32 8537
AlwNI CAG_nnn'CTG
Cuts at: 2147 3596 4556 6312 7884 2147
Size: 1449 960 1756 1572 2832
Fragments arranged by size:
2832 1756 1572 1449 960
ApaLI G'TGCA_C
Cuts at: 6626 7782 6626
Size: 1156 7413
ApoI r'AATT_y
Cuts at: 1493 2301 2924 3033 3146 3170 3284 4053 4113
Size: 808 623 109 113 24 114 769 60
Cuts at: 4113 4463 5405 5796 5878 6104 6256 8365 1493
Size: 350 942 391 82 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 808 769 623 391 350
226 152 114 113 109 82 60 24
AseI AT'TA_AT
Cuts at: 160 1032 2438 3057 3321 7123 160
Size: 872 1406 619 264 3802 1606
Fragments arranged by size:
3802 1606 1406 872 619 264
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
AvaI C'yCGr_G
Cuts at: 1870 1943 3350 5486 5894 1870
Size: 73 1407 2136 408 4545
Fragments arranged by size:
4545 2136 1407 408 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4137 4170 4137
Size: 33 8536
BamHI G'GATC_C
Cuts at: 5506 5899 6266 5506
Size: 393 367 7809
Fragments arranged by size:
7809 393 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2078 5276 5890 7345
Size: 325 217 627 231 60 3198 614 1455
Cuts at: 7345 618
Size: 1842
Fragments arranged by size:
3198 1842 1455 627 614 325 231 217
60
BanII G_rGCy'C
Cuts at: 1391 2129 2242 3770 5888 1391
Size: 738 113 1528 2118 4072
Fragments arranged by size:
4072 2118 1528 738 113
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 8569
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7598
BbvCI CC'TCA_GC
Cuts at: 4650 4650
Size: 8569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6760 6794 1903
Size: 34 50 34 4739 34 3678
Fragments arranged by size:
4739 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2569 6461 7671 2569
Size: 3892 1210 3467
Fragments arranged by size:
3892 3467 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7180 136
Size: 107 122 71 631 605 5508 1525
Fragments arranged by size:
5508 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2417 5380 5565 8564 2417
Size: 2963 185 2999 2422
Fragments arranged by size:
2999 2963 2422 185
BlpI GC'TnA_GC
Cuts at: 5986 5986
Size: 8569
Bme1580I G_kGCm'C
Cuts at: 1790 6630 7786 1790
Size: 4840 1156 2573
Fragments arranged by size:
4840 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3895 1318
Size: 2577 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3505 6300 7258
Size: 416 53 83 399 24 2088 2795 958
Cuts at: 7258 442
Size: 1753
Fragments arranged by size:
2795 2088 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2256 3266 7229 1239
Size: 960 57 1010 3963 2579
Fragments arranged by size:
3963 2579 1010 960 57
Bpu10I CC'TnA_GC
Cuts at: 2100 3573 4650 2100
Size: 1473 1077 6019
Fragments arranged by size:
6019 1473 1077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4685 4919 6694 7559 7857 4685
Size: 234 1775 865 298 5397
Fragments arranged by size:
5397 1775 865 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7232 882
Size: 617 5733 2219
Fragments arranged by size:
5733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6360 493
Size: 711 5156 2702
Fragments arranged by size:
5156 2702 711
BsaBI GATnn'nnATC
Cuts at: 3528 8563 3528
Size: 5035 3534
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 5478 6376 6758
Size: 53 83 186 936 546 3399 898 382
Cuts at: 6758 7393 275
Size: 635 1451
Fragments arranged by size:
3399 1451 936 898 635 546 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7002 7400 7674 7821 8232 7002
Size: 398 274 147 411 7339
Fragments arranged by size:
7339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8344
Fragments arranged by size:
8344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2193 2232 2862 3170 4065 5598 1186
Size: 1007 39 630 308 895 1533 4157
Fragments arranged by size:
4157 1533 1007 895 630 308 39
BseYI C'CCAG_C
Cuts at: 2022 2128 2291 7772 8268 2022
Size: 106 163 5481 496 2323
Fragments arranged by size:
5481 2323 496 163 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 6019 6780 7808 1540
Size: 275 186 115 3903 761 1028 2301
Fragments arranged by size:
3903 2301 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 2242 2407 3770 6630 6715 7786 2242
Size: 165 1363 2860 85 1071 3025
Fragments arranged by size:
3025 2860 1363 1071 165 85
BsmI GAATG_Cn'
Cuts at: 2196 6080 6173 2196
Size: 3884 93 4592
Fragments arranged by size:
4592 3884 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2242 2407 3770 5888 6630 6715
Size: 399 339 113 165 1363 2118 742 85
Cuts at: 6715 7786 1391
Size: 1071 2174
Fragments arranged by size:
2174 2118 1363 1071 742 399 339 165
113 85
BspEI T'CCGG_A
Cuts at: 8232 8232
Size: 8569
BspHI T'CATG_A
Cuts at: 3037 3733 5254 6458 8188 3037
Size: 696 1521 1204 1730 3418
Fragments arranged by size:
3418 1730 1521 1204 696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3307 5405 5924 844
Size: 2463 2098 519 3489
Fragments arranged by size:
3489 2463 2098 519
BsrBI CCG'CTC
Cuts at: 1394 1823 6456 1394
Size: 429 4633 3507
Fragments arranged by size:
4633 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2226 3783 4380 5076 7064 7238 65
Size: 1183 978 1557 597 696 1988 174 1396
Fragments arranged by size:
1988 1557 1396 1183 978 696 597 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7213 7400 1700
Size: 110 5403 187 2869
Fragments arranged by size:
5403 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2477 96
Size: 2381 6188
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8569
BssSI C'ACGA_G
Cuts at: 3226 6629 7641 3226
Size: 3403 1012 4154
Fragments arranged by size:
4154 3403 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2147 4576 794
Size: 1353 2429 4787
Fragments arranged by size:
4787 2429 1353
BstBI TT'CG_AA
Cuts at: 2644 5758 7373 2644
Size: 3114 1615 3840
Fragments arranged by size:
3840 3114 1615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8569
BstXI CCAn_nnnn'nTGG
Cuts at: 3244 3244
Size: 8569
BstYI r'GATC_y
Cuts at: 1972 2417 4959 5188 5380 5506 5565 5899 6266
Size: 445 2542 229 192 126 59 334 367
Cuts at: 6266 6651 6668 8120 8206 8218 8564 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2542 1977 1452 445 385 367 346 334
229 192 126 86 59 17 12
BstZ17I GTA'TAC
Cuts at: 3296 3296
Size: 8569
BtgI C'CryG_G
Cuts at: 513 1065 2650 513
Size: 552 1585 6432
Fragments arranged by size:
6432 1585 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4958 5266 487
Size: 683 56 3732 308 3790
Fragments arranged by size:
3790 3732 683 308 56
BtsI GCAGTG_nn'
Cuts at: 2031 2359 3558 5132 6085 6879 6899 2031
Size: 328 1199 1574 953 794 20 3701
Fragments arranged by size:
3701 1574 1199 953 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8534
DraI TTT'AAA
Cuts at: 3289 3721 5686 5873 3289
Size: 432 1965 187 5985
Fragments arranged by size:
5985 1965 432 187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7576 809
Size: 507 6260 1802
Fragments arranged by size:
6260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 6016 6905
Size: 54 1425 50 275 18 283 3903 889
Cuts at: 6905 8534 8
Size: 1629 43
Fragments arranged by size:
3903 1629 1425 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 6016 1537
Size: 275 301 3903 4090
Fragments arranged by size:
4090 3903 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 3059 4320 5028 5157 5628 5760 6499
Size: 822 884 1261 708 129 471 132 739
Cuts at: 6499 1353
Size: 3423
Fragments arranged by size:
3423 1261 884 822 739 708 471 132
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7156 7530 7676 387
Size: 1064 138 5567 374 146 1280
Fragments arranged by size:
5567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2240 3768 2240
Size: 1528 7041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2256 3266 3410 4341 5538 6632
Size: 393 567 57 1010 144 931 1197 1094
Cuts at: 6632 7229 8016 8284 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1197 1094 1010 931 787 597 567
393 268 144 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3353 3407 4808 3353
Size: 54 1401 7114
Fragments arranged by size:
7114 1401 54
EcoO109I rG'GnC_Cy
Cuts at: 2309 2309
Size: 8569
EcoRI G'AATT_C
Cuts at: 5878 6256 5878
Size: 378 8191
EcoRV GAT'ATC
Cuts at: 3526 3526
Size: 8569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 4418 4736 5054 2911
Size: 1507 318 318 6426
Fragments arranged by size:
6426 1507 318 318
FspI TGC'GCA
Cuts at: 7075 7075
Size: 8569
HaeII r_GCGC'y
Cuts at: 1678 2082 2326 7716 1678
Size: 404 244 5390 2531
Fragments arranged by size:
5390 2531 404 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 5191 5223 5498
Size: 32 135 32 521 32 3066 32 275
Cuts at: 5498 5530 7210 7242 7284 7316 1373
Size: 32 1680 32 42 32 2626
Fragments arranged by size:
3066 2626 1680 521 275 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5913 6159 669
Size: 988 281 3975 246 3079
Fragments arranged by size:
3975 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4122 748
Size: 3374 5195
HpaI GTT'AAC
Cuts at: 6159 6159
Size: 8569
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 8569
KpnI G_GTAC'C
Cuts at: 5894 5894
Size: 8569
MfeI C'AATT_G
Cuts at: 6168 6168
Size: 8569
MluI A'CGCG_T
Cuts at: 8445 8445
Size: 8569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2611 2798 5537 7683 7867 1305
Size: 1306 187 2739 2146 184 2007
Fragments arranged by size:
2739 2146 2007 1306 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6747
Fragments arranged by size:
6747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2601 6527 6886 7045 518
Size: 648 1435 3926 359 159 2042
Fragments arranged by size:
3926 2042 1435 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 5985 6662 7810 8055 1606
Size: 4379 677 1148 245 2120
Fragments arranged by size:
4379 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 8569
NcoI C'CATG_G
Cuts at: 513 1065 2650 513
Size: 552 1585 6432
Fragments arranged by size:
6432 1585 552
NdeI CA'TA_TG
Cuts at: 387 5374 387
Size: 4987 3582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8569
NotI GC'GGCC_GC
Cuts at: 6016 6016
Size: 8569
NspI r_CATG'y
Cuts at: 1132 1229 3224 5021 5927 7472 1132
Size: 97 1995 1797 906 1545 2229
Fragments arranged by size:
2229 1995 1797 1545 906 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8569
PciI A'CATG_T
Cuts at: 7468 7468
Size: 8569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8569
PfoI T'CCnGG_A
Cuts at: 3841 3841
Size: 8569
PmeI GTTT'AAAC
Cuts at: 5873 5873
Size: 8569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6638 6670 8151 8183 6638
Size: 32 1481 32 7024
Fragments arranged by size:
7024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2309 2309
Size: 8569
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8569
PsiI TTA'TAA
Cuts at: 6139 6139
Size: 8569
PspXI vC'TCGA_Gb
Cuts at: 5486 5486
Size: 8569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4380 4412 4380
Size: 32 8537
PstI C_TGCA'G
Cuts at: 830 2576 3553 4942 5921 830
Size: 1746 977 1389 979 3478
Fragments arranged by size:
3478 1746 1389 979 977
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8569
SacI G_AGCT'C
Cuts at: 2242 3770 2242
Size: 1528 7041
SalI G'TCGA_C
Cuts at: 1936 5911 1936
Size: 3975 4594
SapI GCTCTTCn'nnn_
Cuts at: 3059 5028 5628 3059
Size: 1969 600 6000
Fragments arranged by size:
6000 1969 600
SbfI CC_TGCA'GG
Cuts at: 5921 5921
Size: 8569
ScaI AGT'ACT
Cuts at: 6817 8529 6817
Size: 1712 6857
SfcI C'TryA_G
Cuts at: 826 1045 2572 3549 4548 4938 5142 5917 7052
Size: 219 1527 977 999 390 204 775 1135
Cuts at: 7052 7733 7924 826
Size: 681 191 1471
Fragments arranged by size:
1527 1471 1135 999 977 775 681 390
219 204 191
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 8569
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8569
SmaI CCC'GGG
Cuts at: 1872 5896 1872
Size: 4024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3350 4700 4898 5486 6673 7574
Size: 197 926 1407 1350 198 588 1187 901
Cuts at: 7574 7836 820
Size: 262 1553
Fragments arranged by size:
1553 1407 1350 1187 926 901 588 262
198 197
SnaBI TAC'GTA
Cuts at: 493 6360 493
Size: 5867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8569
SphI G_CATG'C
Cuts at: 1132 5927 1132
Size: 4795 3774
SspI AAT'ATT
Cuts at: 5 52 2762 2832 2949 2978 3991 4505 5105
Size: 47 2710 70 117 29 1013 514 600
Cuts at: 5105 6493 5
Size: 1388 2081
Fragments arranged by size:
2710 2081 1388 1013 600 514 117 70
47 29
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8569
StyI C'CwwG_G
Cuts at: 513 1065 2650 2934 6008 513
Size: 552 1585 284 3074 3074
Fragments arranged by size:
3074 3074 1585 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 4064 6596 6613 6766 6951 7396
Size: 1255 456 2028 2532 17 153 185 445
Cuts at: 7396 325
Size: 1498
Fragments arranged by size:
2532 2028 1498 1255 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2477 4396 4540
Size: 274 80 33 51 1443 500 1919 144
Cuts at: 4540 6815 8527 96
Size: 2275 1712 138
Fragments arranged by size:
2275 1919 1712 1443 500 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5234 5266 8529 8561 5234
Size: 32 3263 32 5242
Fragments arranged by size:
5242 3263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4823 1316
Size: 3507 5062
XbaI T'CTAG_A
Cuts at: 5905 5905
Size: 8569
XhoI C'TCGA_G
Cuts at: 1943 3350 5486 1943
Size: 1407 2136 5026
Fragments arranged by size:
5026 2136 1407
XmaI C'CCGG_G
Cuts at: 1870 5894 1870
Size: 4024 4545
XmnI GAAnn'nnTTC
Cuts at: 2535 3033 3195 4294 5382 6698 2535
Size: 498 162 1099 1088 1316 4406
Fragments arranged by size:
4406 1316 1099 1088 498 162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6377 276
Size: 53 83 186 936 4843 2468
Fragments arranged by size:
4843 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI
Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PvuII RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI