################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII BanI BbeI BcnI BglI BseAI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FseI FspI HgaI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 2480 GACGTC GACGT^C AccII 3334 CGCG CG^CG AcyI 2480 GACGTC G[GA]^CG[CT]C AflII 3022 CTTAAG C^TTAAG AocI 1774 CCTGAGG CC^T[ACGT]AGG AvaI 2915 CTCGAG C^[CT]CG[GA]G AvrII 3457 CCTAGG C^CTAGG BalI 3155 TGGCCA TGG^CCA BamHI 314 GGATCC G^GATCC BbrPI 67 CACGTG CAC^GTG BcgI 2685 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1701 GGATAC GTATCC(6/5) BfrI 3022 CTTAAG C^TTAAG BlnI 3457 CCTAGG C^CTAGG BlpI 3271 GCTCAGC GC^T[ACGT]AGC BpmI 1517 CTGGAG CTGGAG(16/14) Bpu1102I 3271 GCTCAGC GC^T[ACGT]AGC BsaAI 67 CACGTG [CT]AC^GT[GA] BsaBI 2333 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2480 GACGTC G[GA]^CG[CT]C BsaWI 3604 ACCGGA [AT]^CCGG[AT] BseRI 970 GAGGAG GAGGAG(10/8) BsoBI 2915 CTCGAG C^[CT]CG[GA]G Bsp1407I 143 TGTACA T^GTACA BspDI 2334 ATCGAT AT^CGAT BspLU11I 1651 ACATGT A^CATGT BsrDI 1222 GCAATG GCAATG(2/0) BsrGI 143 TGTACA T^GTACA BstUI 3334 CGCG CG^CG BstXI 772 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1774 CCTGAGG CC^T[ACGT]AGG CelII 3271 GCTCAGC GC^T[ACGT]AGC ClaI 2334 ATCGAT AT^CGAT DraI 3517 TTTAAA TTT^AAA DrdI 3525 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1763 CCATGG C^C[GA][CT]GG EaeI 3155 TGGCCA [CT]^GGCC[GA] Eco81I 1774 CCTGAGG CC^T[ACGT]AGG EcoT22I 422 ATGCAT ATGCA^T Hin1I 2480 GACGTC G[GA]^CG[CT]C MamI 2333 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3155 TGGCCA TGG^CCA MscI 3155 TGGCCA TGG^CCA MslI 773 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 3334 CGCG CG^CG NcoI 1763 CCATGG C^CATGG NsiI 422 ATGCAT ATGCA^T NspI 1651 ACATGT [GA]CATG^[CT] PacI 1750 TTAATTAA TTAAT^TAA PaeR7I 2915 CTCGAG C^TCGAG PflMI 3366 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 67 CACGTG CAC^GTG PmlI 67 CACGTG CAC^GTG SapI 2518 GAAGAGC GCTCTTC(1/4) SspBI 143 TGTACA T^GTACA Tsp45I 2023 GTCAC ^GT[GC]AC Van91I 3366 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3143 CCAAGACTCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2915 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 66 ACACGT A^C[GA][CT]GT 1651 ACATGT Asp700I 557 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2197 GAAACTATTC BbvCI 2113 CCTCAGC CCTCAGC(-5/-2) 1696 GCTGAGG BclI 258 TGATCA T^GATCA 1622 TGATCA BsgI 3233 CTGCAC GTGCAG(16/14) 332 GTGCAG BsmI 3538 GAATGC GAATGC(1/-1) 3597 GCATTC BspHI 705 TCATGA T^CATGA 2906 TCATGA BspMI 999 ACCTGC ACCTGC(4/8) 334 GCAGGT BssSI 540 CACGAG CACGAG(-5/-1) 3031 CACGAG BstAPI 343 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3342 GCATCAGCTGC DraII 77 GGGACCT [GA]G^G[ACGT]CC[CT] 1770 GGGTCCT EcoO109I 77 GGGACCT [GA]G^G[ACGT]CC[CT] 1770 GGGTCCT EcoRV 502 GATATC GAT^ATC 2337 GATATC EcoT14I 1763 CCATGG C^C[AT][AT]GG 3457 CCTAGG FbaI 258 TGATCA T^GATCA 1622 TGATCA HaeII 2582 GGCGCT [GA]GCGC^[CT] 3026 AGCGCC HphI 1915 GGTGA GGTGA(8/7) 261 TCACC PpuMI 77 GGGACCT [GA]G^G[AT]CC[CT] 1770 GGGTCCT PstI 859 CTGCAG CTGCA^G 3349 CTGCAG RcaI 705 TCATGA T^CATGA 2906 TCATGA SacI 2912 GAGCTC GAGCT^C 2935 GAGCTC StyI 1763 CCATGG C^C[AT][AT]GG 3457 CCTAGG XbaI 282 TCTAGA T^CTAGA 2640 TCTAGA XmnI 557 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2197 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanII BbsI BbvI BfaI BglII BmyI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII