(Circular) (Six-base) MAPSORT of: pFN21AE3390.seq Check: 7575 from: 1 to: 8317 pFN21AE3390 8317 bp With 182 enzymes: SgfI * January 18, 2013 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2330 2330 Size: 8317 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4489 6127 278 Size: 53 83 186 936 2953 1638 2468 Fragments arranged by size: 2953 2468 1638 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5660 1937 Size: 3723 4594 Acc65I G'GTAC_C Cuts at: 5638 5638 Size: 8317 AclI AA'CG_TT Cuts at: 1563 6444 6817 1563 Size: 4881 373 3063 Fragments arranged by size: 4881 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2492 2622 2952 3069 3141 3489 3936 Size: 600 260 130 330 117 72 348 447 Cuts at: 3936 3969 4107 4485 4499 4790 5297 5423 6380 Size: 33 138 378 14 291 507 126 957 Cuts at: 6380 7764 8032 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 957 600 507 447 378 348 330 291 268 260 138 130 126 117 72 33 14 AflII C'TTAA_G Cuts at: 820 1017 5027 820 Size: 197 4010 4110 Fragments arranged by size: 4110 4010 197 AflIII A'CryG_T Cuts at: 1203 2071 3656 7216 8193 1203 Size: 868 1585 3560 977 1327 Fragments arranged by size: 3560 1585 1327 977 868 AgeI A'CCGG_T Cuts at: 7148 7148 Size: 8317 AhdI GACnn_n'nnGTC Cuts at: 7046 7046 Size: 8317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8317 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3993 4025 4566 4598 3993 Size: 32 541 32 7712 Fragments arranged by size: 7712 541 32 32 AlwNI CAG_nnn'CTG Cuts at: 2863 4223 4259 5401 6060 7632 2863 Size: 1360 36 1142 659 1572 3548 Fragments arranged by size: 3548 1572 1360 1142 659 36 ApaLI G'TGCA_C Cuts at: 6374 7530 6374 Size: 1156 7161 ApoI r'AATT_y Cuts at: 1493 2330 2794 2989 3141 4059 4294 4394 4578 Size: 837 464 195 152 918 235 100 184 Cuts at: 4578 4754 5579 5626 5852 6004 8113 1493 Size: 176 825 47 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 837 825 464 235 226 195 184 176 152 152 100 47 AseI AT'TA_AT Cuts at: 160 1032 3195 3336 3755 3759 4143 6871 160 Size: 872 2163 141 419 4 384 2728 1606 Fragments arranged by size: 2728 2163 1606 872 419 384 141 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 AvaI C'yCGr_G Cuts at: 1870 1943 4920 5642 1870 Size: 73 2977 722 4545 Fragments arranged by size: 4545 2977 722 73 AvrII C'CTAG_G Cuts at: 5462 5462 Size: 8317 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3019 3052 3019 Size: 33 8284 BamHI G'GATC_C Cuts at: 2319 5647 6014 2319 Size: 3328 367 4622 Fragments arranged by size: 4622 3328 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5638 7093 618 Size: 325 217 627 3851 1455 1842 Fragments arranged by size: 3851 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4537 4921 4944 5636 1391 Size: 3146 384 23 692 4072 Fragments arranged by size: 4072 3146 692 384 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 2781 3321 3585 3609 3699 4369 Size: 971 204 678 540 264 24 90 670 Cuts at: 4369 928 Size: 4876 Fragments arranged by size: 4876 971 678 670 540 264 204 90 24 BbvCI CC'TCA_GC Cuts at: 3702 4119 3702 Size: 417 7900 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4679 4713 6508 6542 1903 Size: 34 2742 34 1795 34 3678 Fragments arranged by size: 3678 2742 1795 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3700 6209 7419 3700 Size: 2509 1210 4598 Fragments arranged by size: 4598 2509 1210 BclI T'GATC_A Cuts at: 1548 2263 3627 1548 Size: 715 1364 6238 Fragments arranged by size: 6238 1364 715 BfrBI ATG'CAT Cuts at: 2429 2429 Size: 8317 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6928 136 Size: 107 122 71 631 605 5256 1525 Fragments arranged by size: 5256 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2028 2467 5308 8312 2028 Size: 439 2841 3004 2033 Fragments arranged by size: 3004 2841 2033 439 BlpI GC'TnA_GC Cuts at: 5277 5734 5277 Size: 457 7860 Bme1580I G_kGCm'C Cuts at: 1790 6378 7534 1790 Size: 4588 1156 2573 Fragments arranged by size: 4588 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8317 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2132 4131 5541 Size: 416 53 83 399 24 715 1999 1410 Cuts at: 5541 6048 7006 442 Size: 507 958 1753 Fragments arranged by size: 1999 1753 1410 958 715 507 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8317 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 5292 3109 Size: 2183 6134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3543 6977 1239 Size: 2304 3434 2579 Fragments arranged by size: 3434 2579 2304 Bpu10I CC'TnA_GC Cuts at: 3702 4119 3702 Size: 417 7900 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 3498 5091 6442 7307 7605 2393 Size: 1105 1593 1351 865 298 3105 Fragments arranged by size: 3105 1593 1351 1105 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2162 2371 5452 6980 882 Size: 617 663 209 3081 1528 2219 Fragments arranged by size: 3081 2219 1528 663 617 209 BsaAI yAC'GTr Cuts at: 493 1204 2074 6108 493 Size: 711 870 4034 2702 Fragments arranged by size: 4034 2702 870 711 BsaBI GATnn'nnATC Cuts at: 4342 8311 4342 Size: 3969 4348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4486 6124 6506 7141 Size: 53 83 186 936 2953 1638 382 635 Cuts at: 7141 275 Size: 1451 Fragments arranged by size: 2953 1638 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5609 6750 7148 7422 7569 7980 5609 Size: 1141 398 274 147 411 5946 Fragments arranged by size: 5946 1141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8092 Fragments arranged by size: 8092 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2990 1186 Size: 1804 6513 BseYI C'CCAG_C Cuts at: 5594 7520 8016 5594 Size: 1926 496 5895 Fragments arranged by size: 5895 1926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 5223 1163 Size: 573 622 2865 4257 Fragments arranged by size: 4257 2865 622 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5767 6528 7556 1540 Size: 275 186 3766 761 1028 2301 Fragments arranged by size: 3766 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4692 4921 4944 6378 6463 7534 4692 Size: 229 23 1434 85 1071 5475 Fragments arranged by size: 5475 1434 1071 229 85 23 BsmI GAATG_Cn' Cuts at: 5549 5602 5828 5921 5549 Size: 53 226 93 7945 Fragments arranged by size: 7945 226 93 53 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4537 4692 4921 4944 5636 6378 6463 Size: 399 2747 155 229 23 692 742 85 Cuts at: 6463 7534 1391 Size: 1071 2174 Fragments arranged by size: 2747 2174 1071 742 692 399 229 155 85 23 BspEI T'CCGG_A Cuts at: 7980 7980 Size: 8317 BspHI T'CATG_A Cuts at: 2710 4911 6206 7936 2710 Size: 2201 1295 1730 3091 Fragments arranged by size: 3091 2201 1730 1295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 3013 5672 844 Size: 1486 683 2659 3489 Fragments arranged by size: 3489 2659 1486 683 BsrBI CCG'CTC Cuts at: 1394 1823 6204 1394 Size: 429 4381 3507 Fragments arranged by size: 4381 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3234 6812 6986 65 Size: 1183 1986 3578 174 1396 Fragments arranged by size: 3578 1986 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6961 7148 1700 Size: 110 5151 187 2869 Fragments arranged by size: 5151 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2148 96 Size: 2052 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8317 BssSI C'ACGA_G Cuts at: 2545 5036 6377 7389 2545 Size: 2491 1341 1012 3473 Fragments arranged by size: 3473 2491 1341 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 5353 794 Size: 1560 2999 3758 Fragments arranged by size: 3758 2999 1560 BstBI TT'CG_AA Cuts at: 7121 7121 Size: 8317 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8317 BstXI CCAn_nnnn'nTGG Cuts at: 2784 2784 Size: 8317 BstYI r'GATC_y Cuts at: 1972 2028 2319 2467 5308 5647 6014 6399 6416 Size: 56 291 148 2841 339 367 385 17 Cuts at: 6416 7868 7954 7966 8312 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2841 1977 1452 385 367 346 339 291 148 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3780 3780 Size: 8317 BtgI C'CryG_G Cuts at: 513 1065 3768 513 Size: 552 2703 5062 Fragments arranged by size: 5062 2703 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7578 Fragments arranged by size: 7578 683 56 BtsI GCAGTG_nn' Cuts at: 4266 5146 5327 5833 6627 6647 4266 Size: 880 181 506 794 20 5936 Fragments arranged by size: 5936 880 794 506 181 20 ClaI AT'CG_AT Cuts at: 1554 4340 1554 Size: 2786 5531 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8282 DraI TTT'AAA Cuts at: 5524 5621 5524 Size: 97 8220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 7324 809 Size: 507 4220 1788 1802 Fragments arranged by size: 4220 1802 1788 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5160 5764 6653 Size: 54 1425 50 275 18 3330 604 889 Cuts at: 6653 8282 8 Size: 1629 43 Fragments arranged by size: 3330 1629 1425 889 604 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5764 1537 Size: 275 3952 4090 Fragments arranged by size: 4090 3952 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2557 2967 3275 4518 5168 5478 6247 Size: 719 485 410 308 1243 650 310 769 Cuts at: 6247 1353 Size: 3423 Fragments arranged by size: 3423 1243 769 719 650 485 410 310 308 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2534 6904 7278 7424 387 Size: 1064 138 945 4370 374 146 1280 Fragments arranged by size: 4370 1280 1064 945 374 146 138 EcoICRI GAG'CTC Cuts at: 4919 4942 4919 Size: 23 8294 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2492 2622 2952 3069 3141 3489 Size: 393 600 260 130 330 117 72 348 Cuts at: 3489 3543 3936 3969 4107 4485 4499 4790 5297 Size: 54 393 33 138 378 14 291 507 Cuts at: 5297 5423 6380 6977 7764 8032 1239 Size: 126 957 597 787 268 1524 Fragments arranged by size: 1524 957 787 600 597 507 393 393 378 348 330 291 268 260 138 130 126 117 72 54 33 14 EcoO109I rG'GnC_Cy Cuts at: 2083 3776 2083 Size: 1693 6624 EcoRI G'AATT_C Cuts at: 5626 6004 5626 Size: 378 7939 EcoRV GAT'ATC Cuts at: 2509 4344 2509 Size: 1835 6482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2237 2836 3077 3584 2237 Size: 599 241 507 6970 Fragments arranged by size: 6970 599 507 241 FspI TGC'GCA Cuts at: 6823 6823 Size: 8317 HaeII r_GCGC'y Cuts at: 1678 4591 5035 7464 1678 Size: 2913 444 2429 2531 Fragments arranged by size: 2913 2531 2429 444 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2645 2677 2839 Size: 32 135 32 620 32 421 32 162 Cuts at: 2839 2871 4322 4354 6958 6990 7032 7064 1373 Size: 32 1451 32 2604 32 42 32 2626 Fragments arranged by size: 2626 2604 1451 620 421 162 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5661 5907 669 Size: 988 281 3723 246 3079 Fragments arranged by size: 3723 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2282 3290 3455 748 Size: 1534 1008 165 5610 Fragments arranged by size: 5610 1534 1008 165 HpaI GTT'AAC Cuts at: 5907 5907 Size: 8317 KpnI G_GTAC'C Cuts at: 5642 5642 Size: 8317 MfeI C'AATT_G Cuts at: 5916 5916 Size: 8317 MluI A'CGCG_T Cuts at: 8193 8193 Size: 8317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2916 7431 7615 1305 Size: 1611 4515 184 2007 Fragments arranged by size: 4515 2007 1611 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5162 10 Size: 54 1425 343 3330 3165 Fragments arranged by size: 3330 3165 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2782 6275 6634 6793 518 Size: 648 1616 3493 359 159 2042 Fragments arranged by size: 3493 2042 1616 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2860 4975 5353 5733 6410 7558 7803 1606 Size: 1254 2115 378 380 677 1148 245 2120 Fragments arranged by size: 2120 2115 1254 1148 677 380 378 245 NcoI C'CATG_G Cuts at: 513 1065 3768 513 Size: 552 2703 5062 Fragments arranged by size: 5062 2703 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8317 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8317 NotI GC'GGCC_GC Cuts at: 5764 5764 Size: 8317 NsiI A_TGCA'T Cuts at: 2431 2431 Size: 8317 NspI r_CATG'y Cuts at: 1132 1229 3660 5675 7220 1132 Size: 97 2431 2015 1545 2229 Fragments arranged by size: 2431 2229 2015 1545 97 PacI TTA_AT'TAA Cuts at: 3759 3759 Size: 8317 PasI CC'CwG_GG Cuts at: 1348 2665 1348 Size: 1317 7000 PciI A'CATG_T Cuts at: 3656 7216 3656 Size: 3560 4757 PflMI CCAn_nnn'nTGG Cuts at: 1108 5377 1108 Size: 4269 4048 PmeI GTTT'AAAC Cuts at: 5621 5621 Size: 8317 PmlI CAC'GTG Cuts at: 2074 2074 Size: 8317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6386 6418 7899 7931 6386 Size: 32 1481 32 6772 Fragments arranged by size: 6772 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 3776 2083 Size: 1693 6624 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8317 PsiI TTA'TAA Cuts at: 3461 5887 3461 Size: 2426 5891 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4941 4973 4941 Size: 32 8285 PstI C_TGCA'G Cuts at: 830 2868 5358 5669 830 Size: 2038 2490 311 3478 Fragments arranged by size: 3478 2490 2038 311 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8317 PvuII CAG'CTG Cuts at: 2860 4975 5353 2860 Size: 2115 378 5824 Fragments arranged by size: 5824 2115 378 SacI G_AGCT'C Cuts at: 4921 4944 4921 Size: 23 8294 SalI G'TCGA_C Cuts at: 1936 5659 1936 Size: 3723 4594 SapI GCTCTTCn'nnn_ Cuts at: 4518 4518 Size: 8317 SbfI CC_TGCA'GG Cuts at: 5669 5669 Size: 8317 ScaI AGT'ACT Cuts at: 6565 8277 6565 Size: 1712 6605 SfcI C'TryA_G Cuts at: 826 1045 2864 4095 4559 5354 5501 5665 6800 Size: 219 1819 1231 464 795 147 164 1135 Cuts at: 6800 7481 7672 826 Size: 681 191 1471 Fragments arranged by size: 1819 1471 1231 1135 795 681 464 219 191 164 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8317 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8317 SmaI CCC'GGG Cuts at: 1872 5644 1872 Size: 3772 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2372 3513 4920 5027 5106 6421 Size: 197 926 429 1141 1407 107 79 1315 Cuts at: 6421 7322 7584 820 Size: 901 262 1553 Fragments arranged by size: 1553 1407 1315 1141 926 901 429 262 197 107 79 SnaBI TAC'GTA Cuts at: 493 6108 493 Size: 5615 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8317 SphI G_CATG'C Cuts at: 1132 5675 1132 Size: 4543 3774 SspI AAT'ATT Cuts at: 5 52 3391 3541 4876 6241 5 Size: 47 3339 150 1335 1365 2081 Fragments arranged by size: 3339 2081 1365 1335 150 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8317 StyI C'CwwG_G Cuts at: 513 1065 3768 5462 5756 513 Size: 552 2703 1694 294 3074 Fragments arranged by size: 3074 2703 1694 552 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 3599 6344 6361 6514 6699 7144 Size: 1255 703 1316 2745 17 153 185 445 Cuts at: 7144 325 Size: 1498 Fragments arranged by size: 2745 1498 1316 1255 703 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 6563 8275 Size: 274 80 33 51 1443 171 4415 1712 Cuts at: 8275 96 Size: 138 Fragments arranged by size: 4415 1712 1443 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2828 2860 8277 8309 2828 Size: 32 5417 32 2836 Fragments arranged by size: 5417 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8317 XbaI T'CTAG_A Cuts at: 2287 4645 5653 2287 Size: 2358 1008 4951 Fragments arranged by size: 4951 2358 1008 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5155 5155 Size: 8317 XhoI C'TCGA_G Cuts at: 1943 4920 1943 Size: 2977 5340 XmaI C'CCGG_G Cuts at: 1870 5642 1870 Size: 3772 4545 XmnI GAAnn'nnTTC Cuts at: 2566 4206 6446 2566 Size: 1640 2240 4437 Fragments arranged by size: 4437 2240 1640 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4487 6125 276 Size: 53 83 186 936 2953 1638 2468 Fragments arranged by size: 2953 2468 1638 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI