(Linear) (Six-base) MAPSORT of: KIEE3390.seq Check: 7446 from: 1 to: 3612 KIEE3390.orf With 182 enzymes: SgfI * January 18, 2013 16:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 325 3612 Size: 325 3287 AatII G_ACGT'C Cuts at: 0 2484 3612 Size: 2484 1128 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 487 617 947 1064 1136 1484 1931 Size: 227 260 130 330 117 72 348 447 Cuts at: 1931 1964 2102 2480 2494 2785 3292 3418 3612 Size: 33 138 378 14 291 507 126 194 Fragments arranged by size: 507 447 378 348 330 291 260 227 194 138 130 126 117 72 33 14 AflII C'TTAA_G Cuts at: 0 3022 3612 Size: 3022 590 AflIII A'CryG_T Cuts at: 0 66 1651 3612 Size: 66 1585 1961 Fragments arranged by size: 1961 1585 66 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1988 2020 2561 2593 3612 Size: 1988 32 541 32 1019 Fragments arranged by size: 1988 1019 541 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 858 2218 2254 3396 3612 Size: 858 1360 36 1142 216 Fragments arranged by size: 1360 1142 858 216 36 ApoI r'AATT_y Cuts at: 0 325 789 984 1136 2054 2289 2389 2573 Size: 325 464 195 152 918 235 100 184 Cuts at: 2573 2749 3574 3612 Size: 176 825 38 Fragments arranged by size: 918 825 464 325 235 195 184 176 152 100 38 AseI AT'TA_AT Cuts at: 0 1190 1331 1750 1754 2138 3612 Size: 1190 141 419 4 384 1474 Fragments arranged by size: 1474 1190 419 384 141 4 AvaI C'yCGr_G Cuts at: 0 2915 3612 Size: 2915 697 AvrII C'CTAG_G Cuts at: 0 3457 3612 Size: 3457 155 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1014 1047 3612 Size: 1014 33 2565 Fragments arranged by size: 2565 1014 33 BamHI G'GATC_C Cuts at: 0 314 3612 Size: 314 3298 BanII G_rGCy'C Cuts at: 0 2532 2916 2939 3612 Size: 2532 384 23 673 Fragments arranged by size: 2532 673 384 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 98 776 1316 1580 1604 1694 2364 3612 Size: 98 678 540 264 24 90 670 1248 Fragments arranged by size: 1248 678 670 540 264 98 90 24 BbvCI CC'TCA_GC Cuts at: 0 1697 2114 3612 Size: 1697 417 1498 Fragments arranged by size: 1697 1498 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2674 2708 3612 Size: 2674 34 904 Fragments arranged by size: 2674 904 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1695 3612 Size: 1695 1917 BclI T'GATC_A Cuts at: 0 258 1622 3612 Size: 258 1364 1990 Fragments arranged by size: 1990 1364 258 BfrBI ATG'CAT Cuts at: 0 424 3612 Size: 424 3188 BglII A'GATC_T Cuts at: 0 23 462 3303 3612 Size: 23 439 2841 309 Fragments arranged by size: 2841 439 309 23 BlpI GC'TnA_GC Cuts at: 0 3272 3612 Size: 3272 340 BmrI ACTGGGnnnn_n' Cuts at: 0 127 2126 3536 3612 Size: 127 1999 1410 76 Fragments arranged by size: 1999 1410 127 76 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1104 3287 3612 Size: 1104 2183 325 Fragments arranged by size: 2183 1104 325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1538 3612 Size: 1538 2074 Bpu10I CC'TnA_GC Cuts at: 0 1697 2114 3612 Size: 1697 417 1498 Fragments arranged by size: 1697 1498 417 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1493 3086 3612 Size: 388 1105 1593 526 Fragments arranged by size: 1593 1105 526 388 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 366 3447 3612 Size: 157 209 3081 165 Fragments arranged by size: 3081 209 165 157 BsaAI yAC'GTr Cuts at: 0 69 3612 Size: 69 3543 BsaBI GATnn'nnATC Cuts at: 0 2337 3612 Size: 2337 1275 BsaHI Gr'CG_yC Cuts at: 0 2481 3612 Size: 2481 1131 BsaWI w'CCGG_w Cuts at: 0 3604 3612 Size: 3604 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 985 3612 Size: 985 2627 BseYI C'CCAG_C Cuts at: 0 3589 3612 Size: 3589 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 353 3218 3612 Size: 353 2865 394 Fragments arranged by size: 2865 394 353 BsiHKAI G_wGCw'C Cuts at: 0 2687 2916 2939 3612 Size: 2687 229 23 673 Fragments arranged by size: 2687 673 229 23 BsmI GAATG_Cn' Cuts at: 0 3544 3597 3612 Size: 3544 53 15 Fragments arranged by size: 3544 53 15 Bsp1286I G_dGCh'C Cuts at: 0 2532 2687 2916 2939 3612 Size: 2532 155 229 23 673 Fragments arranged by size: 2532 673 229 155 23 BspHI T'CATG_A Cuts at: 0 705 2906 3612 Size: 705 2201 706 Fragments arranged by size: 2201 706 705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 325 1008 3612 Size: 325 683 2604 Fragments arranged by size: 2604 683 325 BsrDI GCAATG_nn' Cuts at: 0 1229 3612 Size: 1229 2383 BsrGI T'GTAC_A Cuts at: 0 143 3612 Size: 143 3469 BssSI C'ACGA_G Cuts at: 0 540 3031 3612 Size: 540 2491 581 Fragments arranged by size: 2491 581 540 BstAPI GCAn_nnn'nTGC Cuts at: 0 349 3348 3612 Size: 349 2999 264 Fragments arranged by size: 2999 349 264 BstXI CCAn_nnnn'nTGG Cuts at: 0 779 3612 Size: 779 2833 BstYI r'GATC_y Cuts at: 0 23 314 462 3303 3612 Size: 23 291 148 2841 309 Fragments arranged by size: 2841 309 291 148 23 Bsu36I CC'TnA_GG Cuts at: 0 1775 3612 Size: 1775 1837 BtgI C'CryG_G Cuts at: 0 1763 3612 Size: 1763 1849 BtsI GCAGTG_nn' Cuts at: 0 2261 3141 3322 3612 Size: 2261 880 181 290 Fragments arranged by size: 2261 880 290 181 ClaI AT'CG_AT Cuts at: 0 2335 3612 Size: 2335 1277 DraI TTT'AAA Cuts at: 0 3519 3612 Size: 3519 93 DrdI GACnn_nn'nnGTC Cuts at: 0 3531 3612 Size: 3531 81 EaeI y'GGCC_r Cuts at: 0 3155 3612 Size: 3155 457 EarI CTCTTCn'nnn_ Cuts at: 0 67 552 962 1270 2513 3163 3473 3612 Size: 67 485 410 308 1243 650 310 139 Fragments arranged by size: 1243 650 485 410 310 308 139 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 529 3612 Size: 529 3083 EcoICRI GAG'CTC Cuts at: 0 2914 2937 3612 Size: 2914 23 675 Fragments arranged by size: 2914 675 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 487 617 947 1064 1136 1484 1538 Size: 227 260 130 330 117 72 348 54 Cuts at: 1538 1931 1964 2102 2480 2494 2785 3292 3418 Size: 393 33 138 378 14 291 507 126 Cuts at: 3418 3612 Size: 194 Fragments arranged by size: 507 393 378 348 330 291 260 227 194 138 130 126 117 72 54 33 14 EcoO109I rG'GnC_Cy Cuts at: 0 78 1771 3612 Size: 78 1693 1841 Fragments arranged by size: 1841 1693 78 EcoRV GAT'ATC Cuts at: 0 504 2339 3612 Size: 504 1835 1273 Fragments arranged by size: 1835 1273 504 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 232 831 1072 1579 3612 Size: 232 599 241 507 2033 Fragments arranged by size: 2033 599 507 241 232 HaeII r_GCGC'y Cuts at: 0 2586 3030 3612 Size: 2586 444 582 Fragments arranged by size: 2586 582 444 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 640 672 834 866 2317 2349 Size: 187 32 421 32 162 32 1451 32 Cuts at: 2349 3612 Size: 1263 Fragments arranged by size: 1451 1263 421 187 162 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 277 1285 1450 3612 Size: 277 1008 165 2162 Fragments arranged by size: 2162 1008 277 165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 911 3612 Size: 911 2701 MscI TGG'CCA Cuts at: 0 3157 3612 Size: 3157 455 MslI CAynn'nnrTG Cuts at: 0 777 3612 Size: 777 2835 MspA1I CmG'CkG Cuts at: 0 855 2970 3348 3612 Size: 855 2115 378 264 Fragments arranged by size: 2115 855 378 264 NcoI C'CATG_G Cuts at: 0 1763 3612 Size: 1763 1849 NsiI A_TGCA'T Cuts at: 0 426 3612 Size: 426 3186 NspI r_CATG'y Cuts at: 0 1655 3612 Size: 1655 1957 PacI TTA_AT'TAA Cuts at: 0 1754 3612 Size: 1754 1858 PasI CC'CwG_GG Cuts at: 0 660 3612 Size: 660 2952 PciI A'CATG_T Cuts at: 0 1651 3612 Size: 1651 1961 PflMI CCAn_nnn'nTGG Cuts at: 0 3372 3612 Size: 3372 240 PmlI CAC'GTG Cuts at: 0 69 3612 Size: 69 3543 PpuMI rG'GwC_Cy Cuts at: 0 78 1771 3612 Size: 78 1693 1841 Fragments arranged by size: 1841 1693 78 PsiI TTA'TAA Cuts at: 0 1456 3612 Size: 1456 2156 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2936 2968 3612 Size: 2936 32 644 Fragments arranged by size: 2936 644 32 PstI C_TGCA'G Cuts at: 0 863 3353 3612 Size: 863 2490 259 Fragments arranged by size: 2490 863 259 PvuII CAG'CTG Cuts at: 0 855 2970 3348 3612 Size: 855 2115 378 264 Fragments arranged by size: 2115 855 378 264 SacI G_AGCT'C Cuts at: 0 2916 2939 3612 Size: 2916 23 673 Fragments arranged by size: 2916 673 23 SapI GCTCTTCn'nnn_ Cuts at: 0 2513 3612 Size: 2513 1099 SfcI C'TryA_G Cuts at: 0 859 2090 2554 3349 3496 3612 Size: 859 1231 464 795 147 116 Fragments arranged by size: 1231 859 795 464 147 116 SmlI C'TyrA_G Cuts at: 0 367 1508 2915 3022 3101 3612 Size: 367 1141 1407 107 79 511 Fragments arranged by size: 1407 1141 511 367 107 79 SspI AAT'ATT Cuts at: 0 1386 1536 2871 3612 Size: 1386 150 1335 741 Fragments arranged by size: 1386 1335 741 150 StyI C'CwwG_G Cuts at: 0 1763 3457 3612 Size: 1763 1694 155 Fragments arranged by size: 1763 1694 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 278 1594 3612 Size: 278 1316 2018 Fragments arranged by size: 2018 1316 278 TatI w'GTAC_w Cuts at: 0 143 3612 Size: 143 3469 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 823 855 3612 Size: 823 32 2757 Fragments arranged by size: 2757 823 32 XbaI T'CTAG_A Cuts at: 0 282 2640 3612 Size: 282 2358 972 Fragments arranged by size: 2358 972 282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3150 3612 Size: 3150 462 XhoI C'TCGA_G Cuts at: 0 2915 3612 Size: 2915 697 XmnI GAAnn'nnTTC Cuts at: 0 561 2201 3612 Size: 561 1640 1411 Fragments arranged by size: 1640 1411 561 ZraI GAC'GTC Cuts at: 0 2482 3612 Size: 2482 1130 Enzymes that do cut: AarI AatII AcuI AflII AflIII AloI AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI PacI PasI PciI PflMI PmlI PpuMI PsiI PsrI PstI PvuII SacI SapI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI BanI BbeI BglI Bme1580I BmgBI BmtI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstBI BstEII BstZ17I BtgZI CspCI DraIII EagI EcoNI EcoRI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PfoI PmeI PpiI PshAI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XmaI