(Circular) (Six-base) MAPSORT of: pFN21AE0373.seq Check: 8059 from: 1 to: 6877 pFN21AE0373 6877 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4687 278 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4220 1937 Size: 2283 4594 Acc65I G'GTAC_C Cuts at: 4198 4198 Size: 6877 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2181 3165 4940 6324 6592 1632 Size: 503 46 984 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 984 503 268 46 AfeI AGC'GCT Cuts at: 2303 2303 Size: 6877 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 3350 3926 5776 6753 1203 Size: 2147 576 1850 977 1327 Fragments arranged by size: 2147 1850 1327 977 576 AgeI A'CCGG_T Cuts at: 2810 5708 2810 Size: 2898 3979 AhdI GACnn_n'nnGTC Cuts at: 5606 5606 Size: 6877 AleI CACnn'nnGTG Cuts at: 1166 4097 1166 Size: 2931 3946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6877 AlwNI CAG_nnn'CTG Cuts at: 4620 6192 4620 Size: 1572 5305 ApaLI G'TGCA_C Cuts at: 4934 6090 4934 Size: 1156 5721 ApoI r'AATT_y Cuts at: 1493 2131 3036 3092 3133 3224 3312 3338 4186 Size: 638 905 56 41 91 88 26 848 Cuts at: 4186 4412 4564 6673 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 905 848 638 226 152 91 88 56 41 26 AseI AT'TA_AT Cuts at: 160 1032 5431 160 Size: 872 4399 1606 Fragments arranged by size: 4399 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 4202 1870 Size: 73 2259 4545 Fragments arranged by size: 4545 2259 73 BamHI G'GATC_C Cuts at: 4207 4574 4207 Size: 367 6510 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4198 5653 618 Size: 325 217 627 2411 1455 1842 Fragments arranged by size: 2411 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2169 4196 1391 Size: 778 2027 4072 Fragments arranged by size: 4072 2027 778 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2628 2689 2784 928 Size: 971 729 61 95 5021 Fragments arranged by size: 5021 971 729 95 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2084 2118 5068 5102 1903 Size: 34 147 34 2950 34 3678 Fragments arranged by size: 3678 2950 147 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4769 5979 4769 Size: 1210 5667 BclI T'GATC_A Cuts at: 1548 1548 Size: 6877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2524 5488 136 Size: 107 122 71 631 605 852 2964 1525 Fragments arranged by size: 2964 1525 852 631 605 122 107 71 BglII A'GATC_T Cuts at: 6872 6872 Size: 6877 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2781 4938 6094 1790 Size: 991 2157 1156 2573 Fragments arranged by size: 2573 2157 1156 991 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 3596 4608 Size: 416 53 83 399 24 1147 1032 1012 Cuts at: 4608 5566 442 Size: 958 1753 Fragments arranged by size: 1753 1147 1032 1012 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6877 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3320 3320 Size: 6877 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2282 2293 2337 3315 3408 3498 5537 Size: 793 250 11 44 978 93 90 2039 Cuts at: 5537 1239 Size: 2579 Fragments arranged by size: 2579 2039 978 793 250 93 90 44 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5002 5867 6165 5002 Size: 865 298 5714 Fragments arranged by size: 5714 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2616 5540 882 Size: 617 1117 2924 2219 Fragments arranged by size: 2924 2219 1117 617 BsaAI yAC'GTr Cuts at: 493 1204 4668 493 Size: 711 3464 2702 Fragments arranged by size: 3464 2702 711 BsaBI GATnn'nnATC Cuts at: 3250 6871 3250 Size: 3621 3256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4684 5066 5701 275 Size: 53 83 186 936 3151 382 635 1451 Fragments arranged by size: 3151 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2229 2810 5310 5708 5982 6129 6540 2229 Size: 581 2500 398 274 147 411 2566 Fragments arranged by size: 2566 2500 581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 3267 3297 3546 Size: 30 165 30 1147 30 687 30 249 Cuts at: 3546 3576 1178 Size: 30 4479 Fragments arranged by size: 4479 1147 687 249 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2727 1186 Size: 1084 457 5336 Fragments arranged by size: 5336 1084 457 BseYI C'CCAG_C Cuts at: 2719 6080 6576 2719 Size: 3361 496 3020 Fragments arranged by size: 3361 3020 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4327 5088 6116 1540 Size: 275 186 2326 761 1028 2301 Fragments arranged by size: 2326 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2563 4938 5023 6094 2563 Size: 2375 85 1071 3346 Fragments arranged by size: 3346 2375 1071 85 BsmI GAATG_Cn' Cuts at: 3151 4123 4388 4481 3151 Size: 972 265 93 5547 Fragments arranged by size: 5547 972 265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2563 2781 4196 4938 5023 6094 Size: 399 379 394 218 1415 742 85 1071 Cuts at: 6094 1391 Size: 2174 Fragments arranged by size: 2174 1415 1071 742 399 394 379 218 85 BspEI T'CCGG_A Cuts at: 6540 6540 Size: 6877 BspHI T'CATG_A Cuts at: 4766 6496 4766 Size: 1730 5147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4232 844 Size: 3388 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4764 1394 Size: 429 2941 3507 Fragments arranged by size: 3507 2941 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2573 5372 5546 65 Size: 1183 1325 2799 174 1396 Fragments arranged by size: 2799 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2810 3785 5521 5708 1700 Size: 110 1000 975 1736 187 2869 Fragments arranged by size: 2869 1736 1000 975 187 110 BsrGI T'GTAC_A Cuts at: 96 2657 96 Size: 2561 4316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6877 BssSI C'ACGA_G Cuts at: 4937 5949 4937 Size: 1012 5865 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6877 BstBI TT'CG_AA Cuts at: 5681 5681 Size: 6877 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6877 BstYI r'GATC_y Cuts at: 1972 2970 4150 4207 4574 4959 4976 6428 6514 Size: 998 1180 57 367 385 17 1452 86 Cuts at: 6514 6526 6872 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1180 998 385 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 3812 3812 Size: 6877 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2944 487 Size: 683 56 1718 4420 Fragments arranged by size: 4420 1718 683 56 BtsI GCAGTG_nn' Cuts at: 3672 4393 5187 5207 3672 Size: 721 794 20 5342 Fragments arranged by size: 5342 794 721 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 4181 4181 Size: 6877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5884 809 Size: 507 4568 1802 Fragments arranged by size: 4568 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2346 3783 4324 Size: 54 1425 50 275 18 516 1437 541 Cuts at: 4324 5213 6842 8 Size: 889 1629 43 Fragments arranged by size: 1629 1437 1425 889 541 516 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4324 1537 Size: 275 2512 4090 Fragments arranged by size: 4090 2512 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2214 2417 4807 1353 Size: 650 211 203 2390 3423 Fragments arranged by size: 3423 2390 650 211 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5464 5838 5984 387 Size: 1064 138 3875 374 146 1280 Fragments arranged by size: 3875 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2135 2181 2282 2293 2337 3165 Size: 393 400 103 46 101 11 44 828 Cuts at: 3165 3315 3408 3498 4940 5537 6324 6592 1239 Size: 150 93 90 1442 597 787 268 1524 Fragments arranged by size: 1524 1442 828 787 597 400 393 268 150 103 101 93 90 46 44 11 EcoO109I rG'GnC_Cy Cuts at: 2834 2834 Size: 6877 EcoRI G'AATT_C Cuts at: 2131 4186 4564 2131 Size: 2055 378 4444 Fragments arranged by size: 4444 2055 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4041 4041 Size: 6877 FspI TGC'GCA Cuts at: 5383 5383 Size: 6877 HaeII r_GCGC'y Cuts at: 1678 2016 2305 6024 1678 Size: 338 289 3719 2531 Fragments arranged by size: 3719 2531 338 289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 3546 3578 4047 Size: 32 135 32 674 32 1268 32 469 Cuts at: 4047 4079 5518 5550 5592 5624 1373 Size: 32 1439 32 42 32 2626 Fragments arranged by size: 2626 1439 1268 674 469 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4221 4467 669 Size: 988 281 2283 246 3079 Fragments arranged by size: 3079 2283 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6877 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KpnI G_GTAC'C Cuts at: 4202 4202 Size: 6877 MfeI C'AATT_G Cuts at: 3514 4476 3514 Size: 962 5915 MluI A'CGCG_T Cuts at: 6753 6753 Size: 6877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5991 6175 1305 Size: 4686 184 2007 Fragments arranged by size: 4686 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5055 Fragments arranged by size: 5055 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3992 4097 4835 5194 5353 518 Size: 648 2826 105 738 359 159 2042 Fragments arranged by size: 2826 2042 738 648 359 159 105 MspA1I CmG'CkG Cuts at: 1606 3122 4293 4970 6118 6363 1606 Size: 1516 1171 677 1148 245 2120 Fragments arranged by size: 2120 1516 1171 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6325 NdeI CA'TA_TG Cuts at: 387 4002 387 Size: 3615 3262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6877 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NruI TCG'CGA Cuts at: 3529 3529 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 3354 3930 4235 5780 1132 Size: 97 2125 576 305 1545 2229 Fragments arranged by size: 2229 2125 1545 576 305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 3350 3926 5776 3350 Size: 576 1850 4451 Fragments arranged by size: 4451 1850 576 PflMI CCAn_nnn'nTGG Cuts at: 1108 3449 1108 Size: 2341 4536 PfoI T'CCnGG_A Cuts at: 3292 3889 3292 Size: 597 6280 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4137 4169 4946 4978 6459 6491 4137 Size: 32 777 32 1481 32 4523 Fragments arranged by size: 4523 1481 777 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3835 1613 Size: 2222 4655 PsiI TTA'TAA Cuts at: 4447 4447 Size: 6877 PstI C_TGCA'G Cuts at: 830 3679 4229 830 Size: 2849 550 3478 Fragments arranged by size: 3478 2849 550 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 PvuII CAG'CTG Cuts at: 3122 3122 Size: 6877 SalI G'TCGA_C Cuts at: 1936 4219 1936 Size: 2283 4594 SapI GCTCTTCn'nnn_ Cuts at: 2003 2003 Size: 6877 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 5125 6837 5125 Size: 1712 5165 SexAI A'CCwGG_T Cuts at: 3022 3022 Size: 6877 SfcI C'TryA_G Cuts at: 826 1045 3675 4225 5360 6041 6232 826 Size: 219 2630 550 1135 681 191 1471 Fragments arranged by size: 2630 1471 1135 681 550 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 4204 1872 Size: 2332 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4981 5882 6144 820 Size: 197 926 3038 901 262 1553 Fragments arranged by size: 3038 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 4235 1132 Size: 3103 3774 SspI AAT'ATT Cuts at: 5 52 2386 3569 4801 5 Size: 47 2334 1183 1232 2081 Fragments arranged by size: 2334 2081 1232 1183 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6877 StyI C'CwwG_G Cuts at: 513 1065 3846 4316 513 Size: 552 2781 470 3074 Fragments arranged by size: 3074 2781 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3296 4904 4921 5074 5259 5704 325 Size: 1255 1716 1608 17 153 185 445 1498 Fragments arranged by size: 1716 1608 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2657 5123 6835 Size: 274 80 33 51 1443 680 2466 1712 Cuts at: 6835 96 Size: 138 Fragments arranged by size: 2466 1712 1443 680 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6837 6869 6837 Size: 32 6845 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6877 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6877 XmaI C'CCGG_G Cuts at: 1870 4202 1870 Size: 2332 4545 XmnI GAAnn'nnTTC Cuts at: 3052 5006 3052 Size: 1954 4923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4685 276 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI