(Circular) (Six-base) MAPSORT of: pFN21AE0366.seq Check: 7274 from: 1 to: 6607 pFN21AE0366 6607 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2144 2144 Size: 6607 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2967 3522 3950 1937 Size: 1030 555 428 4594 Fragments arranged by size: 4594 1030 555 428 Acc65I G'GTAC_C Cuts at: 2532 3928 2532 Size: 1396 5211 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2756 2998 3078 3920 4670 6054 6322 Size: 725 399 242 80 842 750 1384 268 Cuts at: 6322 1632 Size: 1917 Fragments arranged by size: 1917 1384 842 750 725 399 268 242 80 AfeI AGC'GCT Cuts at: 2999 2999 Size: 6607 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 2166 2631 3525 5506 6483 1203 Size: 963 465 894 1981 977 1327 Fragments arranged by size: 1981 1327 977 963 894 465 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 2945 1166 Size: 1779 4828 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 1184 Size: 1089 5518 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3545 3577 3545 Size: 32 6575 AlwNI CAG_nnn'CTG Cuts at: 2290 3676 3690 3859 4350 5922 2290 Size: 1386 14 169 491 1572 2975 Fragments arranged by size: 2975 1572 1386 491 169 14 ApaLI G'TGCA_C Cuts at: 2635 2888 4664 5820 2635 Size: 253 1776 1156 3422 Fragments arranged by size: 3422 1776 1156 253 ApoI r'AATT_y Cuts at: 1493 3710 3916 4142 4294 6403 1493 Size: 2217 206 226 152 2109 1697 Fragments arranged by size: 2217 2109 1697 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 2572 3932 1870 Size: 73 629 1360 4545 Fragments arranged by size: 4545 1360 629 73 AvrII C'CTAG_G Cuts at: 2344 2344 Size: 6607 BamHI G'GATC_C Cuts at: 2046 3937 4304 2046 Size: 1891 367 4349 Fragments arranged by size: 4349 1891 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2532 2741 3928 5383 618 Size: 325 217 627 745 209 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 745 627 325 217 209 BanII G_rGCy'C Cuts at: 1391 2321 2513 2579 3926 1391 Size: 930 192 66 1347 4072 Fragments arranged by size: 4072 1347 930 192 66 BbeI G_GCGC'C Cuts at: 2745 2745 Size: 6607 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2752 2821 3202 928 Size: 971 853 69 381 4333 Fragments arranged by size: 4333 971 853 381 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4798 4832 1903 Size: 34 2861 34 3678 Fragments arranged by size: 3678 2861 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4499 5709 4499 Size: 1210 5397 BclI T'GATC_A Cuts at: 1548 3633 1548 Size: 2085 4522 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3729 6602 3729 Size: 2873 3734 BlpI GC'TnA_GC Cuts at: 2061 4024 2061 Size: 1963 4644 Bme1580I G_kGCm'C Cuts at: 1790 2639 2892 3191 3330 3783 4668 5824 1790 Size: 849 253 299 139 453 885 1156 2573 Fragments arranged by size: 2573 1156 885 849 453 299 253 139 BmgBI CAC'GTC Cuts at: 1318 2367 2559 1318 Size: 1049 192 5366 Fragments arranged by size: 5366 1049 192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3754 4338 5296 Size: 416 53 83 399 24 2337 584 958 Cuts at: 5296 442 Size: 1753 Fragments arranged by size: 2337 1753 958 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2110 2561 2926 3529 2110 Size: 451 365 603 5188 Fragments arranged by size: 5188 603 451 365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2264 2288 2473 2857 3795 5267 1239 Size: 848 177 24 185 384 938 1472 2579 Fragments arranged by size: 2579 1472 938 848 384 185 177 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4732 5597 5895 4732 Size: 865 298 5444 Fragments arranged by size: 5444 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2063 2648 5270 882 Size: 617 564 585 2622 2219 Fragments arranged by size: 2622 2219 617 585 564 BsaAI yAC'GTr Cuts at: 493 1204 3526 3603 4398 493 Size: 711 2322 77 795 2702 Fragments arranged by size: 2702 2322 795 711 77 BsaBI GATnn'nnATC Cuts at: 6601 6601 Size: 6607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2742 3318 4414 Size: 53 83 186 936 507 702 576 1096 Cuts at: 4414 4796 5431 275 Size: 382 635 1451 Fragments arranged by size: 1451 1096 936 702 635 576 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5040 5438 5712 5859 6270 5040 Size: 398 274 147 411 5377 Fragments arranged by size: 5377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 2666 2696 3434 Size: 30 165 30 1158 30 75 30 738 Cuts at: 3434 3464 1178 Size: 30 4321 Fragments arranged by size: 4321 1158 738 165 75 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2217 2220 1186 Size: 913 118 3 5573 Fragments arranged by size: 5573 913 118 3 BseYI C'CCAG_C Cuts at: 2793 5810 6306 2793 Size: 3017 496 3094 Fragments arranged by size: 3094 3017 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6034 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 4057 4818 5846 1540 Size: 275 186 101 1955 761 1028 2301 Fragments arranged by size: 2301 1955 1028 761 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2321 2513 2639 2892 3261 3378 3869 4668 4753 Size: 192 126 253 369 117 491 799 85 Cuts at: 4753 5824 2321 Size: 1071 3104 Fragments arranged by size: 3104 1071 799 491 369 253 192 126 117 85 BsmI GAATG_Cn' Cuts at: 4118 4211 4118 Size: 93 6514 BsmBI CGTCTCn'nnnn_ Cuts at: 2534 2534 Size: 6607 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2513 2579 2639 2892 3191 3261 Size: 399 531 192 66 60 253 299 70 Cuts at: 3261 3330 3378 3783 3869 3926 4668 4753 5824 Size: 69 48 405 86 57 742 85 1071 Cuts at: 5824 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 531 405 399 299 253 192 86 85 70 69 66 60 57 48 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 2214 3564 4496 6226 2214 Size: 1350 932 1730 2595 Fragments arranged by size: 2595 1730 1350 932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2144 3060 3158 3962 844 Size: 1300 916 98 804 3489 Fragments arranged by size: 3489 1300 916 804 98 BsrBI CCG'CTC Cuts at: 1394 1823 2467 4494 1394 Size: 429 644 2027 3507 Fragments arranged by size: 3507 2027 644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5102 5276 65 Size: 1183 3854 174 1396 Fragments arranged by size: 3854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3470 5251 5438 1700 Size: 110 1660 1781 187 2869 Fragments arranged by size: 2869 1781 1660 187 110 BsrGI T'GTAC_A Cuts at: 96 2628 2673 96 Size: 2532 45 4030 Fragments arranged by size: 4030 2532 45 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 2015 4667 5679 2015 Size: 2652 1012 2943 Fragments arranged by size: 2943 2652 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2128 2290 794 Size: 1334 162 5111 Fragments arranged by size: 5111 1334 162 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2046 3729 3937 4304 4689 4706 6158 6244 Size: 74 1683 208 367 385 17 1452 86 Cuts at: 6244 6256 6602 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1683 1452 385 367 346 208 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 2644 3120 3180 513 Size: 552 939 193 447 476 60 3940 Fragments arranged by size: 3940 939 552 476 447 193 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3478 487 Size: 683 56 2252 3616 Fragments arranged by size: 3616 2252 683 56 BtsI GCAGTG_nn' Cuts at: 3829 4123 4917 4937 3829 Size: 294 794 20 5499 Fragments arranged by size: 5499 794 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3584 3619 529 Size: 35 3020 35 3517 Fragments arranged by size: 3517 3020 35 35 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3344 5614 809 Size: 507 2028 2270 1802 Fragments arranged by size: 2270 2028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 2955 3436 Size: 54 1425 50 275 18 269 856 481 Cuts at: 3436 3571 4054 4943 6572 8 Size: 135 483 889 1629 43 Fragments arranged by size: 1629 1425 889 856 483 481 275 269 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2099 4054 1537 Size: 275 287 1955 4090 Fragments arranged by size: 4090 1955 287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2771 3266 3281 3650 4537 1353 Size: 1418 495 15 369 887 3423 Fragments arranged by size: 3423 1418 887 495 369 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5194 5568 5714 387 Size: 1064 138 3605 374 146 1280 Fragments arranged by size: 3605 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2319 2511 2319 Size: 192 6415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2264 2288 2357 2473 2756 2857 Size: 393 455 177 24 69 116 283 101 Cuts at: 2857 2998 3078 3795 3920 4670 5267 6054 6322 Size: 141 80 717 125 750 597 787 268 Cuts at: 6322 1239 Size: 1524 Fragments arranged by size: 1524 787 750 717 597 455 393 283 268 177 141 125 116 101 80 69 24 EcoNI CCTnn'n_nnAGG Cuts at: 3173 3173 Size: 6607 EcoO109I rG'GnC_Cy Cuts at: 2341 2857 3337 3694 3895 2341 Size: 516 480 357 201 5053 Fragments arranged by size: 5053 516 480 357 201 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FspI TGC'GCA Cuts at: 2614 5113 2614 Size: 2499 4108 HaeII r_GCGC'y Cuts at: 1678 2176 2745 3001 5754 1678 Size: 498 569 256 2753 2531 Fragments arranged by size: 2753 2531 569 498 256 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 5248 5280 5322 Size: 32 135 32 989 32 2655 32 42 Cuts at: 5322 5354 1373 Size: 32 2626 Fragments arranged by size: 2655 2626 989 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3951 4197 669 Size: 988 281 2013 246 3079 Fragments arranged by size: 3079 2013 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KasI G'GCGC_C Cuts at: 2741 2741 Size: 6607 KpnI G_GTAC'C Cuts at: 2536 3932 2536 Size: 1396 5211 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3138 5721 5905 1305 Size: 1833 2583 184 2007 Fragments arranged by size: 2583 2007 1833 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 3660 Fragments arranged by size: 3660 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2945 3230 4565 4924 5083 518 Size: 648 1779 285 1335 359 159 2042 Fragments arranged by size: 2042 1779 1335 648 359 285 159 MspA1I CmG'CkG Cuts at: 1606 2287 2646 3100 3122 3136 4023 4700 5848 Size: 681 359 454 22 14 887 677 1148 Cuts at: 5848 6093 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 887 681 677 454 359 245 22 14 NaeI GCC'GGC Cuts at: 3472 3472 Size: 6607 NarI GG'CG_CC Cuts at: 2742 2742 Size: 6607 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NgoMIV G'CCGG_C Cuts at: 3470 3470 Size: 6607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 2131 2635 3094 3965 5510 1132 Size: 97 902 504 459 871 1545 2229 Fragments arranged by size: 2229 1545 902 871 504 459 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 2631 5506 2631 Size: 2875 3732 PflMI CCAn_nnn'nTGG Cuts at: 1108 2586 1108 Size: 1478 5129 PfoI T'CCnGG_A Cuts at: 2241 2405 2436 2241 Size: 164 31 6412 Fragments arranged by size: 6412 164 31 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PmlI CAC'GTG Cuts at: 3603 3603 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3337 3694 3895 3337 Size: 357 201 6049 Fragments arranged by size: 6049 357 201 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2255 2287 2255 Size: 32 6575 PstI C_TGCA'G Cuts at: 830 3336 3959 830 Size: 2506 623 3478 Fragments arranged by size: 3478 2506 623 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 3136 3136 Size: 6607 SacI G_AGCT'C Cuts at: 2321 2513 2321 Size: 192 6415 SacII CC_GC'GG Cuts at: 2647 3123 2647 Size: 476 6131 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SanDI GG'GwC_CC Cuts at: 3694 3694 Size: 6607 SapI GCTCTTCn'nnn_ Cuts at: 3266 3281 3266 Size: 15 6592 SbfI CC_TGCA'GG Cuts at: 3336 3959 3336 Size: 623 5984 ScaI AGT'ACT Cuts at: 3443 4855 6567 3443 Size: 1412 1712 3483 Fragments arranged by size: 3483 1712 1412 SfcI C'TryA_G Cuts at: 826 1045 3332 3682 3955 5090 5771 5962 826 Size: 219 2287 350 273 1135 681 191 1471 Fragments arranged by size: 2287 1471 1135 681 350 273 219 191 SfoI GGC'GCC Cuts at: 2743 2743 Size: 6607 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4711 5612 5874 820 Size: 197 926 2768 901 262 1553 Fragments arranged by size: 2768 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 2349 2727 3195 3384 1517 Size: 832 378 468 189 4740 Fragments arranged by size: 4740 832 468 378 189 StyI C'CwwG_G Cuts at: 513 1065 2004 2344 3366 4046 513 Size: 552 939 340 1022 680 3074 Fragments arranged by size: 3074 1022 939 680 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3736 4634 4651 4804 4989 5434 Size: 1255 717 1439 898 17 153 185 445 Cuts at: 5434 325 Size: 1498 Fragments arranged by size: 1498 1439 1255 898 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2628 2673 Size: 274 80 33 51 1443 147 504 45 Cuts at: 2673 3441 4853 6565 96 Size: 768 1412 1712 138 Fragments arranged by size: 1712 1443 1412 768 504 274 147 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6567 6599 6567 Size: 32 6575 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 4736 4736 Size: 6607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI RsrII SexAI SfiI SrfI SwaI XcmI