Restriction map for
pFN21AE0360
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AocI ApaI AscI AseI
AsnI Asp700I AspI AviII AvrII
BamHI BbvCI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI EagI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FseI FspI HincII
HindII HpaI KspI MluI MroI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 1038 GGTACC G^GTACC
AccI 427 GTCTAC GT^[AC][GT]AC
AcsI 1628 GAATTT [GA]^AATT[CT]
AcyI 211 GGCGCC G[GA]^CG[CT]C
AhdI 419 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 341 GGATC GGATC(4/5)
AlwNI 1542 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 896 AGCGCT AGC^GCT
ApoI 1628 GAATTT [GA]^AATT[CT]
Asp718I 1038 GGTACC G^GTACC
AspEI 419 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 773 CTCGGG C^[CT]CG[GA]G
BbeI 211 GGCGCC GGCGC^C
BbsI 144 GAAGAC GAAGAC(2/6)
BcgI 1713 CGATATAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 998 TGATCA T^GATCA
BcnI 991 CCGGG CC^[GC]GG
BfaI 1379 CTAG C^TAG
BglI 902 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 144 GAAGAC GAAGAC(2/6)
BsaBI 1657 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 211 GGCGCC G[GA]^CG[CT]C
BsaI 34 GAGACC GGTCTC(1/5)
BsoBI 773 CTCGGG C^[CT]CG[GA]G
BspDI 1711 ATCGAT AT^CGAT
BspHI 1169 TCATGA T^CATGA
BspLU11I 917 ACATGT A^CATGT
BsrDI 1480 GCAATG GCAATG(2/0)
BssSI 1902 CTCGTG CACGAG(-5/-1)
BstEII 1617 GGTCACC G^GT[ACGT]ACC
BstPI 1617 GGTCACC G^GT[ACGT]ACC
BstYI 341 GGATCT [GA]^GATC[CT]
ClaI 1711 ATCGAT AT^CGAT
DraIII 247 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 82 GACATCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 823 CCATGG C^C[GA][CT]GG
Eam1105I 419 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 896 AGCGCT AGC^GCT
EcoNI 1901 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1617 GGTCACC G^GT[ACGT]ACC
FbaI 998 TGATCA T^GATCA
HgaI 1249 GCGTC GACGC(5/10)
Hin1I 211 GGCGCC G[GA]^CG[CT]C
HindIII 1802 AAGCTT A^AGCTT
KasI 211 GGCGCC G^GCGCC
KpnI 1038 GGTACC GGTAC^C
MaeI 1379 CTAG C^TAG
MamI 1657 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1397 CAATTG C^AATTG
MflI 341 GGATCT [GA]^GATC[CT]
MspA1I 1403 CAGCTG C[AC]G^C[GT]G
MunI 1397 CAATTG C^AATTG
NarI 211 GGCGCC GG^CGCC
NciI 991 CCGGG CC^[GC]GG
NcoI 823 CCATGG C^CATGG
PflMI 246 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 476 CTGCAG CTGCA^G
PvuII 1403 CAGCTG CAG^CTG
RcaI 1169 TCATGA T^CATGA
SfoI 211 GGCGCC GGC^GCC
StuI 1137 AGGCCT AGG^CCT
Van91I 246 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 341 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
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AflIII 917 ACATGT A^C[GA][CT]GT
1886 ACACGT
Alw44I 873 GTGCAC G^TGCAC
1570 GTGCAC
ApaLI 873 GTGCAC G^TGCAC
1570 GTGCAC
BalI 2 TGGCCA TGG^CCA
826 TGGCCA
BanI 211 GGCGCC G^G[CT][GA]CC
1038 GGTACC
BanII 1173 GAGCTC G[GA]GC[CT]^C
1261 GAGCTC
BbrPI 925 CACGTG CAC^GTG
1784 CACGTG
BsgI 1080 CTGCAC GTGCAG(16/14)
892 GTGCAG
BsmAI 34 GAGAC GTCTC(1/5)
921 GTCTC
Bsp1407I 1013 TGTACA T^GTACA
1659 TGTACA
BsrGI 1013 TGTACA T^GTACA
1659 TGTACA
DpnII 342 GATC ^GATC
999 GATC
HapII 385 CCGG C^CGG
991 CCGG
HpaII 385 CCGG C^CGG
991 CCGG
MboI 342 GATC ^GATC
999 GATC
MluNI 2 TGGCCA TGG^CCA
826 TGGCCA
MscI 2 TGGCCA TGG^CCA
826 TGGCCA
MspI 385 CCGG C^CGG
991 CCGG
MwoI 556 GCTATGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
902 GCCTGTGTGGC
NdeII 342 GATC ^GATC
999 GATC
NspI 917 ACATGT [GA]CATG^[CT]
1030 GCATGT
PmaCI 925 CACGTG CAC^GTG
1784 CACGTG
PmlI 925 CACGTG CAC^GTG
1784 CACGTG
PpuMI 1319 GGGTCCC [GA]G^G[AT]CC[CT]
1833 GGGACCC
SacI 1173 GAGCTC GAGCT^C
1261 GAGCTC
Sau3AI 342 GATC ^GATC
999 GATC
SfcI 161 CTATAG C^T[GA][CT]AG
476 CTGCAG
SspBI 1013 TGTACA T^GTACA
1659 TGTACA
TaqI 731 TCGA T^CGA
1712 TCGA
Tsp45I 808 GTGAC ^GT[GC]AC
1618 GTCAC
TthHB8I 731 TCGA T^CGA
1712 TCGA
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BmyI BpmI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboII MnlI
MseI MslI MvaI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI