(Circular) (Six-base) MAPSORT of: pFN21AE0360.seq Check: 6205 from: 1 to: 6682 pFN21AE0360 6682 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4492 278 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 4025 1937 Size: 496 1592 4594 Fragments arranged by size: 4594 1592 496 Acc65I G'GTAC_C Cuts at: 3043 4003 3043 Size: 960 5722 AclI AA'CG_TT Cuts at: 1563 4809 5182 1563 Size: 3246 373 3063 Fragments arranged by size: 3246 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2268 2984 4745 6129 6397 1632 Size: 536 100 716 1761 1384 268 1917 Fragments arranged by size: 1917 1761 1384 716 536 268 100 AfeI AGC'GCT Cuts at: 2903 2903 Size: 6682 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 2922 3891 5581 6558 1203 Size: 1719 969 1690 977 1327 Fragments arranged by size: 1719 1690 1327 977 969 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 2429 5411 2429 Size: 2982 3700 AleI CACnn'nnGTG Cuts at: 1166 3110 1166 Size: 1944 4738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6682 AlwNI CAG_nnn'CTG Cuts at: 3552 4425 5997 3552 Size: 873 1572 4237 Fragments arranged by size: 4237 1572 873 ApaLI G'TGCA_C Cuts at: 2878 3575 4739 5895 2878 Size: 697 1164 1156 3665 Fragments arranged by size: 3665 1164 1156 697 ApoI r'AATT_y Cuts at: 1493 3633 3991 4217 4369 6478 1493 Size: 2140 358 226 152 2109 1697 Fragments arranged by size: 2140 2109 1697 358 226 152 AseI AT'TA_AT Cuts at: 160 1032 5236 160 Size: 872 4204 1606 Fragments arranged by size: 4204 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 2778 4007 1870 Size: 73 835 1229 4545 Fragments arranged by size: 4545 1229 835 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3656 3689 3656 Size: 33 6649 BamHI G'GATC_C Cuts at: 4012 4379 4012 Size: 367 6315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 3043 4003 5458 618 Size: 325 217 627 429 827 960 1455 1842 Fragments arranged by size: 1842 1455 960 827 627 429 325 217 BanII G_rGCy'C Cuts at: 1391 3182 3270 4001 1391 Size: 1791 88 731 4072 Fragments arranged by size: 4072 1791 731 88 BbeI G_GCGC'C Cuts at: 2220 2220 Size: 6682 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 928 Size: 971 257 5454 Fragments arranged by size: 5454 971 257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3707 3741 4873 4907 1903 Size: 34 1770 34 1132 34 3678 Fragments arranged by size: 3678 1770 1132 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4574 5784 4574 Size: 1210 5472 BclI T'GATC_A Cuts at: 1548 3003 1548 Size: 1455 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2913 5293 136 Size: 107 122 71 631 605 1241 2380 1525 Fragments arranged by size: 2380 1525 1241 631 605 122 107 71 BglII A'GATC_T Cuts at: 6677 6677 Size: 6682 BlpI GC'TnA_GC Cuts at: 4099 4099 Size: 6682 Bme1580I G_kGCm'C Cuts at: 1790 2882 3579 4743 5899 1790 Size: 1092 697 1164 1156 2573 Fragments arranged by size: 2573 1164 1156 1092 697 BmgBI CAC'GTC Cuts at: 1318 3894 1318 Size: 2576 4106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2595 2826 3540 Size: 416 53 83 399 24 1178 231 714 Cuts at: 3540 4413 5371 442 Size: 873 958 1753 Fragments arranged by size: 1753 1178 958 873 714 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3922 3922 Size: 6682 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2517 2606 5342 1239 Size: 1172 106 89 2736 2579 Fragments arranged by size: 2736 2579 1172 106 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 3785 3892 4807 5672 5970 2384 Size: 1401 107 915 865 298 3096 Fragments arranged by size: 3096 1401 915 865 298 107 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2033 5345 882 Size: 617 534 3312 2219 Fragments arranged by size: 3312 2219 617 534 BsaAI yAC'GTr Cuts at: 493 1204 2713 2932 3791 4473 493 Size: 711 1509 219 859 682 2702 Fragments arranged by size: 2702 1509 859 711 682 219 BsaBI GATnn'nnATC Cuts at: 3666 6676 3666 Size: 3010 3672 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 4489 4871 5506 Size: 53 83 186 936 684 2272 382 635 Cuts at: 5506 275 Size: 1451 Fragments arranged by size: 2272 1451 936 684 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5115 5513 5787 5934 6345 5115 Size: 398 274 147 411 5452 Fragments arranged by size: 5452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6457 Fragments arranged by size: 6457 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3722 3872 3875 3932 1186 Size: 2536 150 3 57 3936 Fragments arranged by size: 3936 2536 150 57 3 BseYI C'CCAG_C Cuts at: 2024 2267 3684 5885 6381 2024 Size: 243 1417 2201 496 2325 Fragments arranged by size: 2325 2201 1417 496 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2918 3070 1163 Size: 573 1182 152 4775 Fragments arranged by size: 4775 1182 573 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4132 4893 5921 1540 Size: 275 186 2131 761 1028 2301 Fragments arranged by size: 2301 2131 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2882 3182 3270 3579 4743 4828 5899 2882 Size: 300 88 309 1164 85 1071 3665 Fragments arranged by size: 3665 1164 1071 309 300 88 85 BsmI GAATG_Cn' Cuts at: 2722 2856 3059 3458 4193 4286 2722 Size: 134 203 399 735 93 5118 Fragments arranged by size: 5118 735 399 203 134 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2882 3182 3270 3579 4001 4743 4828 Size: 399 1092 300 88 309 422 742 85 Cuts at: 4828 5899 1391 Size: 1071 2174 Fragments arranged by size: 2174 1092 1071 742 422 399 309 300 88 85 BspEI T'CCGG_A Cuts at: 6345 6345 Size: 6682 BspHI T'CATG_A Cuts at: 3174 4571 6301 3174 Size: 1397 1730 3555 Fragments arranged by size: 3555 1730 1397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4037 844 Size: 3193 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4569 1394 Size: 429 2746 3507 Fragments arranged by size: 3507 2746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3492 5177 5351 65 Size: 1183 2244 1685 174 1396 Fragments arranged by size: 2244 1685 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5326 5513 1700 Size: 110 3516 187 2869 Fragments arranged by size: 3516 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3018 3664 96 Size: 2922 646 3114 Fragments arranged by size: 3114 2922 646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 3907 4742 5754 3907 Size: 835 1012 4835 Fragments arranged by size: 4835 1012 835 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6682 BstBI TT'CG_AA Cuts at: 5486 5486 Size: 6682 BstEII G'GTnAC_C Cuts at: 1184 3622 1184 Size: 2438 4244 BstYI r'GATC_y Cuts at: 1972 2346 4012 4379 4764 4781 6233 6319 6331 Size: 374 1666 367 385 17 1452 86 12 Cuts at: 6331 6677 1972 Size: 346 1977 Fragments arranged by size: 1977 1666 1452 385 374 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2828 513 Size: 552 939 824 4367 Fragments arranged by size: 4367 939 824 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2156 487 Size: 683 56 930 5013 Fragments arranged by size: 5013 930 683 56 BtsI GCAGTG_nn' Cuts at: 4198 4992 5012 4198 Size: 794 20 5868 Fragments arranged by size: 5868 794 20 ClaI AT'CG_AT Cuts at: 1554 3717 1554 Size: 2163 4519 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2040 2075 529 Size: 35 1476 35 5136 Fragments arranged by size: 5136 1476 35 35 DraI TTT'AAA Cuts at: 3986 3986 Size: 6682 DraIII CAC_nnn'GTG Cuts at: 2257 2257 Size: 6682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2093 5689 809 Size: 507 777 3596 1802 Fragments arranged by size: 3596 1802 777 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2207 2831 Size: 54 1425 50 275 18 177 200 624 Cuts at: 2831 4129 5018 6647 8 Size: 1298 889 1629 43 Fragments arranged by size: 1629 1425 1298 889 624 275 200 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4129 1537 Size: 275 2317 4090 Fragments arranged by size: 4090 2317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3221 3726 4612 1353 Size: 1106 762 505 886 3423 Fragments arranged by size: 3423 1106 886 762 505 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2798 5269 5643 5789 387 Size: 1064 138 1209 2471 374 146 1280 Fragments arranged by size: 2471 1280 1209 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3180 3268 3180 Size: 88 6594 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2268 2411 2517 2606 2984 4745 Size: 393 536 100 143 106 89 378 1761 Cuts at: 4745 5342 6129 6397 1239 Size: 597 787 268 1524 Fragments arranged by size: 1761 1524 787 597 536 393 378 268 143 106 100 89 EcoNI CCTnn'n_nnAGG Cuts at: 3910 3910 Size: 6682 EcoO109I rG'GnC_Cy Cuts at: 3325 3839 3903 3325 Size: 514 64 6104 Fragments arranged by size: 6104 514 64 EcoRI G'AATT_C Cuts at: 3991 4369 3991 Size: 378 6304 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 6682 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 2220 2905 3207 5829 1678 Size: 542 685 302 2622 2531 Fragments arranged by size: 2622 2531 685 542 302 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 3345 3377 5323 Size: 32 135 32 843 32 898 32 1946 Cuts at: 5323 5355 5397 5429 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1946 898 843 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4026 4272 669 Size: 988 281 2088 246 3079 Fragments arranged by size: 3079 2088 988 281 246 HindIII A'AGCT_T Cuts at: 748 3807 748 Size: 3059 3623 HpaI GTT'AAC Cuts at: 4272 4272 Size: 6682 KasI G'GCGC_C Cuts at: 2216 2216 Size: 6682 KpnI G_GTAC'C Cuts at: 3047 4007 3047 Size: 960 5722 MfeI C'AATT_G Cuts at: 3402 4281 3402 Size: 879 5803 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2662 3237 3457 5796 5980 1305 Size: 1357 575 220 2339 184 2007 Fragments arranged by size: 2339 2007 1357 575 220 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2833 10 Size: 54 1425 343 177 824 3859 Fragments arranged by size: 3859 1425 824 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2308 2462 2633 2990 3026 3110 3239 Size: 648 1142 154 171 357 36 84 129 Cuts at: 3239 3500 3793 4640 4999 5158 518 Size: 261 293 847 359 159 2042 Fragments arranged by size: 2042 1142 847 648 359 357 293 261 171 159 154 129 84 36 MspA1I CmG'CkG Cuts at: 1606 3410 4098 4775 5923 6168 1606 Size: 1804 688 677 1148 245 2120 Fragments arranged by size: 2120 1804 1148 688 677 245 NarI GG'CG_CC Cuts at: 2217 2217 Size: 6682 NcoI C'CATG_G Cuts at: 513 1065 2004 2828 513 Size: 552 939 824 4367 Fragments arranged by size: 4367 939 824 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6682 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NspI r_CATG'y Cuts at: 1132 1229 2926 3039 4040 5585 1132 Size: 97 1697 113 1001 1545 2229 Fragments arranged by size: 2229 1697 1545 1001 113 97 PasI CC'CwG_GG Cuts at: 1348 3628 1348 Size: 2280 4402 PciI A'CATG_T Cuts at: 2922 5581 2922 Size: 2659 4023 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 5533 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PmlI CAC'GTG Cuts at: 2932 3791 2932 Size: 859 5823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4751 4783 6264 6296 4751 Size: 32 1481 32 5137 Fragments arranged by size: 5137 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3325 3839 3325 Size: 514 6168 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6682 PsiI TTA'TAA Cuts at: 4252 4252 Size: 6682 PstI C_TGCA'G Cuts at: 830 2485 4034 830 Size: 1655 1549 3478 Fragments arranged by size: 3478 1655 1549 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 PvuII CAG'CTG Cuts at: 3410 3410 Size: 6682 SacI G_AGCT'C Cuts at: 3182 3270 3182 Size: 88 6594 SalI G'TCGA_C Cuts at: 1936 4024 1936 Size: 2088 4594 SanDI GG'GwC_CC Cuts at: 3325 3839 3325 Size: 514 6168 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 4930 6642 4930 Size: 1712 4970 SfcI C'TryA_G Cuts at: 826 1045 2166 2481 4030 5165 5846 6037 826 Size: 219 1121 315 1549 1135 681 191 1471 Fragments arranged by size: 1549 1471 1135 1121 681 315 219 191 SfoI GGC'GCC Cuts at: 2218 2218 Size: 6682 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 4009 1872 Size: 2137 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3800 3871 4786 5687 5949 Size: 197 926 456 1401 71 915 901 262 Cuts at: 5949 820 Size: 1553 Fragments arranged by size: 1553 1401 926 915 901 456 262 197 71 SnaBI TAC'GTA Cuts at: 493 4473 493 Size: 3980 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SspI AAT'ATT Cuts at: 5 52 4606 5 Size: 47 4554 2081 Fragments arranged by size: 4554 2081 47 StuI AGG'CCT Cuts at: 1517 3144 1517 Size: 1627 5055 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 2828 3130 3391 3852 4121 Size: 552 939 516 308 302 261 461 269 Cuts at: 4121 513 Size: 3074 Fragments arranged by size: 3074 939 552 516 461 308 302 269 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2331 3145 4709 4726 4879 5064 5509 Size: 1255 751 814 1564 17 153 185 445 Cuts at: 5509 325 Size: 1498 Fragments arranged by size: 1564 1498 1255 814 751 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3018 3585 3664 Size: 274 80 33 51 1443 1041 567 79 Cuts at: 3664 3701 4928 6640 96 Size: 37 1227 1712 138 Fragments arranged by size: 1712 1443 1227 1041 567 274 138 80 79 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6642 6674 6642 Size: 32 6650 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6682 XbaI T'CTAG_A Cuts at: 4018 4018 Size: 6682 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2983 3112 3943 2590 Size: 393 129 831 5329 Fragments arranged by size: 5329 831 393 129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 4007 1870 Size: 2137 4545 XmnI GAAnn'nnTTC Cuts at: 4811 4811 Size: 6682 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4490 276 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI