Restriction map for
pFN21AE0360
(Linear) (Six-base) MAPSORT of: KIEE0360.seq Check: 3726 from: 1 to: 1977
KIEE0360.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 428 1977
Size: 428 1549
Acc65I G'GTAC_C
Cuts at: 0 1038 1977
Size: 1038 939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 263 979 1977
Size: 163 100 716 998
Fragments arranged by size:
998 716 163 100
AfeI AGC'GCT
Cuts at: 0 898 1977
Size: 898 1079
AflIII A'CryG_T
Cuts at: 0 917 1886 1977
Size: 917 969 91
Fragments arranged by size:
969 917 91
AhdI GACnn_n'nnGTC
Cuts at: 0 424 1977
Size: 424 1553
AleI CACnn'nnGTG
Cuts at: 0 1105 1977
Size: 1105 872
AlwNI CAG_nnn'CTG
Cuts at: 0 1547 1977
Size: 1547 430
ApaLI G'TGCA_C
Cuts at: 0 873 1570 1977
Size: 873 697 407
Fragments arranged by size:
873 697 407
ApoI r'AATT_y
Cuts at: 0 1628 1977
Size: 1628 349
AvaI C'yCGr_G
Cuts at: 0 773 1977
Size: 773 1204
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1651 1684 1977
Size: 1651 33 293
Fragments arranged by size:
1651 293 33
BanI G'GyrC_C
Cuts at: 0 211 1038 1977
Size: 211 827 939
Fragments arranged by size:
939 827 211
BanII G_rGCy'C
Cuts at: 0 1177 1265 1977
Size: 1177 88 712
Fragments arranged by size:
1177 712 88
BbeI G_GCGC'C
Cuts at: 0 215 1977
Size: 215 1762
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 1977
Size: 151 1826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1702 1736 1977
Size: 1702 34 241
Fragments arranged by size:
1702 241 34
BclI T'GATC_A
Cuts at: 0 998 1977
Size: 998 979
BglI GCCn_nnn'nGGC
Cuts at: 0 908 1977
Size: 908 1069
Bme1580I G_kGCm'C
Cuts at: 0 877 1574 1977
Size: 877 697 403
Fragments arranged by size:
877 697 403
BmgBI CAC'GTC
Cuts at: 0 1889 1977
Size: 1889 88
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 821 1535 1977
Size: 590 231 714 442
Fragments arranged by size:
714 590 442 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1917 1977
Size: 1917 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 512 601 1977
Size: 406 106 89 1376
Fragments arranged by size:
1376 406 106 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1780 1887 1977
Size: 379 1401 107 90
Fragments arranged by size:
1401 379 107 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 28 1977
Size: 28 1949
BsaAI yAC'GTr
Cuts at: 0 708 927 1786 1977
Size: 708 219 859 191
Fragments arranged by size:
859 708 219 191
BsaBI GATnn'nnATC
Cuts at: 0 1661 1977
Size: 1661 316
BsaHI Gr'CG_yC
Cuts at: 0 212 1977
Size: 212 1765
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1717 1867 1870 1927 1977
Size: 1717 150 3 57 50
Fragments arranged by size:
1717 150 57 50 3
BseYI C'CCAG_C
Cuts at: 0 19 262 1679 1977
Size: 19 243 1417 298
Fragments arranged by size:
1417 298 243 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1065 1977
Size: 913 152 912
Fragments arranged by size:
913 912 152
BsiHKAI G_wGCw'C
Cuts at: 0 877 1177 1265 1574 1977
Size: 877 300 88 309 403
Fragments arranged by size:
877 403 309 300 88
BsmI GAATG_Cn'
Cuts at: 0 717 851 1054 1453 1977
Size: 717 134 203 399 524
Fragments arranged by size:
717 524 399 203 134
Bsp1286I G_dGCh'C
Cuts at: 0 877 1177 1265 1574 1977
Size: 877 300 88 309 403
Fragments arranged by size:
877 403 309 300 88
BspHI T'CATG_A
Cuts at: 0 1169 1977
Size: 1169 808
BsrDI GCAATG_nn'
Cuts at: 0 1487 1977
Size: 1487 490
BsrGI T'GTAC_A
Cuts at: 0 1013 1659 1977
Size: 1013 646 318
Fragments arranged by size:
1013 646 318
BssSI C'ACGA_G
Cuts at: 0 1902 1977
Size: 1902 75
BstEII G'GTnAC_C
Cuts at: 0 1617 1977
Size: 1617 360
BstYI r'GATC_y
Cuts at: 0 341 1977
Size: 341 1636
BtgI C'CryG_G
Cuts at: 0 823 1977
Size: 823 1154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 151 1977
Size: 151 1826
ClaI AT'CG_AT
Cuts at: 0 1712 1977
Size: 1712 265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 35 70 1977
Size: 35 35 1907
Fragments arranged by size:
1907 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 252 1977
Size: 252 1725
DrdI GACnn_nn'nnGTC
Cuts at: 0 88 1977
Size: 88 1889
EaeI y'GGCC_r
Cuts at: 0 2 202 826 1977
Size: 2 200 624 1151
Fragments arranged by size:
1151 624 200 2
EarI CTCTTCn'nnn_
Cuts at: 0 454 1216 1721 1977
Size: 454 762 505 256
Fragments arranged by size:
762 505 454 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 793 1977
Size: 793 1184
EcoICRI GAG'CTC
Cuts at: 0 1175 1263 1977
Size: 1175 88 714
Fragments arranged by size:
1175 714 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 263 406 512 601 979 1977
Size: 163 100 143 106 89 378 998
Fragments arranged by size:
998 378 163 143 106 100 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1905 1977
Size: 1905 72
EcoO109I rG'GnC_Cy
Cuts at: 0 1320 1834 1898 1977
Size: 1320 514 64 79
Fragments arranged by size:
1320 514 79 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 531 1977
Size: 531 1446
HaeII r_GCGC'y
Cuts at: 0 215 900 1202 1977
Size: 215 685 302 775
Fragments arranged by size:
775 685 302 215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 1340 1372 1977
Size: 410 32 898 32 605
Fragments arranged by size:
898 605 410 32 32
HindIII A'AGCT_T
Cuts at: 0 1802 1977
Size: 1802 175
KasI G'GCGC_C
Cuts at: 0 211 1977
Size: 211 1766
KpnI G_GTAC'C
Cuts at: 0 1042 1977
Size: 1042 935
MfeI C'AATT_G
Cuts at: 0 1397 1977
Size: 1397 580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 1232 1452 1977
Size: 657 575 220 525
Fragments arranged by size:
657 575 525 220
MscI TGG'CCA
Cuts at: 0 4 828 1977
Size: 4 824 1149
Fragments arranged by size:
1149 824 4
MslI CAynn'nnrTG
Cuts at: 0 303 457 628 985 1021 1105 1234 1495
Size: 303 154 171 357 36 84 129 261
Cuts at: 1495 1788 1977
Size: 293 189
Fragments arranged by size:
357 303 293 261 189 171 154 129
84 36
MspA1I CmG'CkG
Cuts at: 0 1405 1977
Size: 1405 572
NarI GG'CG_CC
Cuts at: 0 212 1977
Size: 212 1765
NcoI C'CATG_G
Cuts at: 0 823 1977
Size: 823 1154
NspI r_CATG'y
Cuts at: 0 921 1034 1977
Size: 921 113 943
Fragments arranged by size:
943 921 113
PasI CC'CwG_GG
Cuts at: 0 1623 1977
Size: 1623 354
PciI A'CATG_T
Cuts at: 0 917 1977
Size: 917 1060
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1977
Size: 252 1725
PmlI CAC'GTG
Cuts at: 0 927 1786 1977
Size: 927 859 191
Fragments arranged by size:
927 859 191
PpuMI rG'GwC_Cy
Cuts at: 0 1320 1834 1977
Size: 1320 514 143
Fragments arranged by size:
1320 514 143
PstI C_TGCA'G
Cuts at: 0 480 1977
Size: 480 1497
PvuII CAG'CTG
Cuts at: 0 1405 1977
Size: 1405 572
SacI G_AGCT'C
Cuts at: 0 1177 1265 1977
Size: 1177 88 712
Fragments arranged by size:
1177 712 88
SanDI GG'GwC_CC
Cuts at: 0 1320 1834 1977
Size: 1320 514 143
Fragments arranged by size:
1320 514 143
SfcI C'TryA_G
Cuts at: 0 161 476 1977
Size: 161 315 1501
Fragments arranged by size:
1501 315 161
SfoI GGC'GCC
Cuts at: 0 213 1977
Size: 213 1764
SmlI C'TyrA_G
Cuts at: 0 394 1795 1866 1977
Size: 394 1401 71 111
Fragments arranged by size:
1401 394 111 71
StuI AGG'CCT
Cuts at: 0 1139 1977
Size: 1139 838
StyI C'CwwG_G
Cuts at: 0 515 823 1125 1386 1847 1977
Size: 515 308 302 261 461 130
Fragments arranged by size:
515 461 308 302 261 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 326 1140 1977
Size: 326 814 837
Fragments arranged by size:
837 814 326
TatI w'GTAC_w
Cuts at: 0 1013 1580 1659 1696 1977
Size: 1013 567 79 37 281
Fragments arranged by size:
1013 567 281 79 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 978 1107 1938 1977
Size: 585 393 129 831 39
Fragments arranged by size:
831 585 393 129 39
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI
BcgI BclI BglI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BssSI BstEII
BstYI BtgI BtgZI ClaI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PciI PflMI PmlI
PpuMI PstI PvuII SacI SanDI SfcI SfoI SmlI
StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AlfI AloI ApaI
AscI AseI AsiSI AvrII BamHI BbvCI BciVI BfrBI
BglII BlpI BmtI Bpu10I BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI
BstXI BstZ17I Bsu36I BtsI DraI EagI EcoRI EcoRV
FseI FspI FspAI HincII HpaI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI