(Circular) (Six-base) MAPSORT of: pFN21AE0349.seq Check: 4086 from: 1 to: 6859 pFN21AE0349 6859 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4202 1937 Size: 2265 4594 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2802 3782 3798 3825 4922 6306 6574 1632 Size: 1170 980 16 27 1097 1384 268 1917 Fragments arranged by size: 1917 1384 1170 1097 980 268 27 16 AfeI AGC'GCT Cuts at: 3395 3395 Size: 6859 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 2655 5758 6735 1203 Size: 1452 3103 977 1327 Fragments arranged by size: 3103 1452 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6859 AlwNI CAG_nnn'CTG Cuts at: 2216 2572 4602 6174 2216 Size: 356 2030 1572 2901 Fragments arranged by size: 2901 2030 1572 356 ApaLI G'TGCA_C Cuts at: 4916 6072 4916 Size: 1156 5703 ApoI r'AATT_y Cuts at: 1493 2252 2713 4131 4168 4394 4546 6655 1493 Size: 759 461 1418 37 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 759 461 226 152 37 AseI AT'TA_AT Cuts at: 160 1032 5413 160 Size: 872 4381 1606 Fragments arranged by size: 4381 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 3216 4184 1870 Size: 73 1273 968 4545 Fragments arranged by size: 4545 1273 968 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2818 2851 2818 Size: 33 6826 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2441 3280 4180 5635 Size: 325 217 627 372 282 839 900 1455 Cuts at: 5635 618 Size: 1842 Fragments arranged by size: 1842 1455 900 839 627 372 325 282 217 BanII G_rGCy'C Cuts at: 1391 4178 1391 Size: 2787 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3786 928 Size: 971 1887 4001 Fragments arranged by size: 4001 1887 971 BbvCI CC'TCA_GC Cuts at: 4010 4010 Size: 6859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5050 5084 1903 Size: 34 3113 34 3678 Fragments arranged by size: 3678 3113 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2111 2961 4751 5961 2111 Size: 850 1790 1210 3009 Fragments arranged by size: 3009 1790 1210 850 BclI T'GATC_A Cuts at: 1548 2580 3264 1548 Size: 1032 684 5143 Fragments arranged by size: 5143 1032 684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 5470 136 Size: 107 122 71 631 605 370 3428 1525 Fragments arranged by size: 3428 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 6854 6854 Size: 6859 BlpI GC'TnA_GC Cuts at: 4276 4276 Size: 6859 Bme1580I G_kGCm'C Cuts at: 1790 2446 2593 3484 4920 6076 1790 Size: 656 147 891 1436 1156 2573 Fragments arranged by size: 2573 1436 1156 891 656 147 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6859 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3350 3842 4590 Size: 416 53 83 399 24 1933 492 748 Cuts at: 4590 5548 442 Size: 958 1753 Fragments arranged by size: 1933 1753 958 748 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3694 3694 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2238 5519 1239 Size: 999 3281 2579 Fragments arranged by size: 3281 2579 999 Bpu10I CC'TnA_GC Cuts at: 4010 4010 Size: 6859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4984 5849 6147 4984 Size: 865 298 5696 Fragments arranged by size: 5696 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3737 4023 5522 882 Size: 617 2238 286 1499 2219 Fragments arranged by size: 2238 2219 1499 617 286 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 3813 6853 3813 Size: 3040 3819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4666 5048 5683 275 Size: 53 83 186 936 3133 382 635 1451 Fragments arranged by size: 3133 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2310 2528 5292 5690 5964 6111 6522 2310 Size: 218 2764 398 274 147 411 2647 Fragments arranged by size: 2764 2647 411 398 274 218 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2523 2553 3592 3622 3881 Size: 30 165 30 1120 30 1039 30 259 Cuts at: 3881 3911 1178 Size: 30 4126 Fragments arranged by size: 4126 1120 1039 259 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3851 4033 4058 1186 Size: 2665 182 25 3987 Fragments arranged by size: 3987 2665 182 25 BseYI C'CCAG_C Cuts at: 2812 6062 6558 2812 Size: 3250 496 3113 Fragments arranged by size: 3250 3113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2873 1163 Size: 573 1137 5149 Fragments arranged by size: 5149 1137 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4309 5070 6098 1540 Size: 275 186 2308 761 1028 2301 Fragments arranged by size: 2308 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 3009 4920 5005 6076 2196 Size: 813 1911 85 1071 2979 Fragments arranged by size: 2979 1911 1071 813 85 BsmI GAATG_Cn' Cuts at: 3717 4370 4463 3717 Size: 653 93 6113 Fragments arranged by size: 6113 653 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2446 2593 3009 3484 4178 4920 Size: 399 406 250 147 416 475 694 742 Cuts at: 4920 5005 6076 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 475 416 406 399 250 147 85 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 2577 4748 6478 2577 Size: 2171 1730 2958 Fragments arranged by size: 2958 2171 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 4214 844 Size: 2992 378 3489 Fragments arranged by size: 3489 2992 378 BsrBI CCG'CTC Cuts at: 1394 1823 4746 1394 Size: 429 2923 3507 Fragments arranged by size: 3507 2923 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2031 2877 3406 5354 5528 65 Size: 1183 783 846 529 1948 174 1396 Fragments arranged by size: 1948 1396 1183 846 783 529 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5503 5690 1700 Size: 110 3693 187 2869 Fragments arranged by size: 3693 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3186 96 Size: 3090 3769 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 4919 5931 4919 Size: 1012 5847 BstAPI GCAn_nnn'nTGC Cuts at: 794 2033 2572 3013 794 Size: 1239 539 441 4640 Fragments arranged by size: 4640 1239 539 441 BstBI TT'CG_AA Cuts at: 3628 5663 3628 Size: 2035 4824 BstEII G'GTnAC_C Cuts at: 1184 3823 1184 Size: 2639 4220 BstYI r'GATC_y Cuts at: 1972 2213 2428 4189 4556 4941 4958 6410 6496 Size: 241 215 1761 367 385 17 1452 86 Cuts at: 6496 6508 6854 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1761 1452 385 367 346 241 215 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3859 3859 Size: 6859 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2244 2550 3007 3117 3736 4375 5169 5189 2244 Size: 306 457 110 619 639 794 20 3914 Fragments arranged by size: 3914 794 639 619 457 306 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3273 3308 529 Size: 35 2709 35 4080 Fragments arranged by size: 4080 2709 35 35 DraI TTT'AAA Cuts at: 3997 4163 3997 Size: 166 6693 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 3546 4306 Size: 54 1425 50 275 18 529 1187 760 Cuts at: 4306 5195 6824 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1187 889 760 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4306 1537 Size: 275 2494 4090 Fragments arranged by size: 4090 2494 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4035 4065 4789 1353 Size: 2682 30 724 3423 Fragments arranged by size: 3423 2682 724 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5446 5820 5966 387 Size: 1064 138 3857 374 146 1280 Fragments arranged by size: 3857 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2802 3782 3798 3825 4922 5519 Size: 393 606 564 980 16 27 1097 597 Cuts at: 5519 6306 6574 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1097 980 787 606 597 564 393 268 27 16 EcoO109I rG'GnC_Cy Cuts at: 2860 3793 2860 Size: 933 5926 EcoRI G'AATT_C Cuts at: 2252 4168 4546 2252 Size: 1916 378 4565 Fragments arranged by size: 4565 1916 378 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 2137 3397 6006 1678 Size: 459 1260 2609 2531 Fragments arranged by size: 2609 2531 1260 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3109 3128 3141 3160 3881 Size: 32 135 32 1537 19 13 19 721 Cuts at: 3881 3913 5500 5532 5574 5606 1373 Size: 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 1537 721 135 42 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3889 4203 4449 669 Size: 988 281 1951 314 246 3079 Fragments arranged by size: 3079 1951 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6859 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 2154 4458 2154 Size: 2304 4555 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2450 2730 2789 5973 6157 1305 Size: 1145 280 59 3184 184 2007 Fragments arranged by size: 3184 2007 1145 280 184 59 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 3548 10 Size: 54 1425 343 529 1187 3321 Fragments arranged by size: 3321 1425 1187 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 3017 4817 5176 5335 518 Size: 648 1338 513 1800 359 159 2042 Fragments arranged by size: 2042 1800 1338 648 513 359 159 MspA1I CmG'CkG Cuts at: 1606 4275 4952 6100 6345 1606 Size: 2669 677 1148 245 2120 Fragments arranged by size: 2669 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6307 NdeI CA'TA_TG Cuts at: 387 387 Size: 6859 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 2659 3016 4217 5762 1132 Size: 97 1430 357 1201 1545 2229 Fragments arranged by size: 2229 1545 1430 1201 357 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 2655 5758 2655 Size: 3103 3756 PflMI CCAn_nnn'nTGG Cuts at: 1108 3642 1108 Size: 2534 4325 PfoI T'CCnGG_A Cuts at: 2454 2963 2454 Size: 509 6350 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4928 4960 6441 6473 4928 Size: 32 1481 32 5314 Fragments arranged by size: 5314 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 6859 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3956 3988 3956 Size: 32 6827 PstI C_TGCA'G Cuts at: 830 2568 3833 4211 830 Size: 1738 1265 378 3478 Fragments arranged by size: 3478 1738 1265 378 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 SalI G'TCGA_C Cuts at: 1936 4201 1936 Size: 2265 4594 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 5107 6819 5107 Size: 1712 5147 SexAI A'CCwGG_T Cuts at: 3657 3657 Size: 6859 SfcI C'TryA_G Cuts at: 826 1045 2564 3829 4207 5342 6023 6214 826 Size: 219 1519 1265 378 1135 681 191 1471 Fragments arranged by size: 1519 1471 1265 1135 681 378 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6859 SmaI CCC'GGG Cuts at: 1872 3218 4186 1872 Size: 1346 968 4545 Fragments arranged by size: 4545 1346 968 SmlI C'TyrA_G Cuts at: 820 1017 1943 4963 5864 6126 820 Size: 197 926 3020 901 262 1553 Fragments arranged by size: 3020 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 3016 4217 1132 Size: 1884 1201 3774 Fragments arranged by size: 3774 1884 1201 SspI AAT'ATT Cuts at: 5 52 2182 3049 4102 4783 5 Size: 47 2130 867 1053 681 2081 Fragments arranged by size: 2130 2081 1053 867 681 47 StuI AGG'CCT Cuts at: 1517 2535 1517 Size: 1018 5841 StyI C'CwwG_G Cuts at: 513 1065 2783 4298 513 Size: 552 1718 1515 3074 Fragments arranged by size: 3074 1718 1515 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3268 3911 4886 4903 5056 5241 5686 Size: 1255 1688 643 975 17 153 185 445 Cuts at: 5686 325 Size: 1498 Fragments arranged by size: 1688 1498 1255 975 643 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2014 3186 5105 Size: 274 80 33 51 1443 37 1172 1919 Cuts at: 5105 6817 96 Size: 1712 138 Fragments arranged by size: 1919 1712 1443 1172 274 138 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 3592 3624 6819 6851 2223 Size: 32 1337 32 3195 32 2231 Fragments arranged by size: 3195 2231 1337 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2843 1316 Size: 1527 5332 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2820 2422 Size: 398 6461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6859 XmaI C'CCGG_G Cuts at: 1870 3216 4184 1870 Size: 1346 968 4545 Fragments arranged by size: 4545 1346 968 XmnI GAAnn'nnTTC Cuts at: 2778 2959 3624 4988 2778 Size: 181 665 1364 4649 Fragments arranged by size: 4649 1364 665 181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI