Restriction map for
pFN21AE0349
(Linear) (Six-base) MAPSORT of: KIEE0349.seq Check: 2261 from: 1 to: 2154
KIEE0349.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1777 1793 1820 2154
Size: 797 980 16 27 334
Fragments arranged by size:
980 797 334 27 16
AfeI AGC'GCT
Cuts at: 0 1390 2154
Size: 1390 764
AflIII A'CryG_T
Cuts at: 0 650 2154
Size: 650 1504
AlwNI CAG_nnn'CTG
Cuts at: 0 211 567 2154
Size: 211 356 1587
Fragments arranged by size:
1587 356 211
ApoI r'AATT_y
Cuts at: 0 247 708 2126 2154
Size: 247 461 1418 28
Fragments arranged by size:
1418 461 247 28
AvaI C'yCGr_G
Cuts at: 0 1211 2154
Size: 1211 943
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 813 846 2154
Size: 813 33 1308
Fragments arranged by size:
1308 813 33
BanI G'GyrC_C
Cuts at: 0 154 436 1275 2154
Size: 154 282 839 879
Fragments arranged by size:
879 839 282 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 1781 2154
Size: 1781 373
BbvCI CC'TCA_GC
Cuts at: 0 2005 2154
Size: 2005 149
BciVI GTATCCnnnnn_n'
Cuts at: 0 106 956 2154
Size: 106 850 1198
Fragments arranged by size:
1198 850 106
BclI T'GATC_A
Cuts at: 0 575 1259 2154
Size: 575 684 895
Fragments arranged by size:
895 684 575
BglI GCCn_nnn'nGGC
Cuts at: 0 37 2154
Size: 37 2117
Bme1580I G_kGCm'C
Cuts at: 0 441 588 1479 2154
Size: 441 147 891 675
Fragments arranged by size:
891 675 441 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 1345 1837 2154
Size: 1345 492 317
Fragments arranged by size:
1345 492 317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1689 2154
Size: 1689 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 2154
Size: 233 1921
Bpu10I CC'TnA_GC
Cuts at: 0 2005 2154
Size: 2005 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 1732 2018 2154
Size: 1732 286 136
Fragments arranged by size:
1732 286 136
BsaBI GATnn'nnATC
Cuts at: 0 1808 2154
Size: 1808 346
BsaWI w'CCGG_w
Cuts at: 0 305 523 2154
Size: 305 218 1631
Fragments arranged by size:
1631 305 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 518 548 1587 1617 1876 1906 2154
Size: 518 30 1039 30 259 30 248
Fragments arranged by size:
1039 518 259 248 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1846 2028 2053 2154
Size: 1846 182 25 101
Fragments arranged by size:
1846 182 101 25
BseYI C'CCAG_C
Cuts at: 0 807 2154
Size: 807 1347
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 2154
Size: 868 1286
BsiHKAI G_wGCw'C
Cuts at: 0 191 1004 2154
Size: 191 813 1150
Fragments arranged by size:
1150 813 191
BsmI GAATG_Cn'
Cuts at: 0 1712 2154
Size: 1712 442
Bsp1286I G_dGCh'C
Cuts at: 0 191 441 588 1004 1479 2154
Size: 191 250 147 416 475 675
Fragments arranged by size:
675 475 416 250 191 147
BspHI T'CATG_A
Cuts at: 0 572 2154
Size: 572 1582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1831 2154
Size: 1831 323
BsrDI GCAATG_nn'
Cuts at: 0 26 872 1401 2154
Size: 26 846 529 753
Fragments arranged by size:
846 753 529 26
BsrGI T'GTAC_A
Cuts at: 0 1181 2154
Size: 1181 973
BstAPI GCAn_nnn'nTGC
Cuts at: 0 28 567 1008 2154
Size: 28 539 441 1146
Fragments arranged by size:
1146 539 441 28
BstBI TT'CG_AA
Cuts at: 0 1623 2154
Size: 1623 531
BstEII G'GTnAC_C
Cuts at: 0 1818 2154
Size: 1818 336
BstYI r'GATC_y
Cuts at: 0 208 423 2154
Size: 208 215 1731
Fragments arranged by size:
1731 215 208
Bsu36I CC'TnA_GG
Cuts at: 0 1854 2154
Size: 1854 300
BtsI GCAGTG_nn'
Cuts at: 0 239 545 1002 1112 1731 2154
Size: 239 306 457 110 619 423
Fragments arranged by size:
619 457 423 306 239 110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1268 1303 2154
Size: 1268 35 851
Fragments arranged by size:
1268 851 35
DraI TTT'AAA
Cuts at: 0 1992 2154
Size: 1992 162
EaeI y'GGCC_r
Cuts at: 0 354 1541 2154
Size: 354 1187 613
Fragments arranged by size:
1187 613 354
EarI CTCTTCn'nnn_
Cuts at: 0 2030 2060 2154
Size: 2030 30 94
Fragments arranged by size:
2030 94 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 797 1777 1793 1820 2154
Size: 233 564 980 16 27 334
Fragments arranged by size:
980 564 334 233 27 16
EcoO109I rG'GnC_Cy
Cuts at: 0 855 1788 2154
Size: 855 933 366
Fragments arranged by size:
933 855 366
EcoRI G'AATT_C
Cuts at: 0 247 2154
Size: 247 1907
HaeII r_GCGC'y
Cuts at: 0 132 1392 2154
Size: 132 1260 762
Fragments arranged by size:
1260 762 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1104 1123 1136 1155 1876 1908 2154
Size: 1104 19 13 19 721 32 246
Fragments arranged by size:
1104 721 246 32 19 19 13
HincII GTy'rAC
Cuts at: 0 1884 2154
Size: 1884 270
MfeI C'AATT_G
Cuts at: 0 149 2154
Size: 149 2005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 725 784 2154
Size: 445 280 59 1370
Fragments arranged by size:
1370 445 280 59
MscI TGG'CCA
Cuts at: 0 356 1543 2154
Size: 356 1187 611
Fragments arranged by size:
1187 611 356
MslI CAynn'nnrTG
Cuts at: 0 499 1012 2154
Size: 499 513 1142
Fragments arranged by size:
1142 513 499
NspI r_CATG'y
Cuts at: 0 654 1011 2154
Size: 654 357 1143
Fragments arranged by size:
1143 654 357
PciI A'CATG_T
Cuts at: 0 650 2154
Size: 650 1504
PflMI CCAn_nnn'nTGG
Cuts at: 0 1637 2154
Size: 1637 517
PfoI T'CCnGG_A
Cuts at: 0 449 958 2154
Size: 449 509 1196
Fragments arranged by size:
1196 509 449
PpuMI rG'GwC_Cy
Cuts at: 0 855 2154
Size: 855 1299
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1951 1983 2154
Size: 1951 32 171
Fragments arranged by size:
1951 171 32
PstI C_TGCA'G
Cuts at: 0 563 1828 2154
Size: 563 1265 326
Fragments arranged by size:
1265 563 326
SexAI A'CCwGG_T
Cuts at: 0 1652 2154
Size: 1652 502
SfcI C'TryA_G
Cuts at: 0 559 1824 2154
Size: 559 1265 330
Fragments arranged by size:
1265 559 330
SmaI CCC'GGG
Cuts at: 0 1213 2154
Size: 1213 941
SphI G_CATG'C
Cuts at: 0 1011 2154
Size: 1011 1143
SspI AAT'ATT
Cuts at: 0 177 1044 2097 2154
Size: 177 867 1053 57
Fragments arranged by size:
1053 867 177 57
StuI AGG'CCT
Cuts at: 0 530 2154
Size: 530 1624
StyI C'CwwG_G
Cuts at: 0 778 2154
Size: 778 1376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1263 1906 2154
Size: 1263 643 248
Fragments arranged by size:
1263 643 248
TatI w'GTAC_w
Cuts at: 0 9 1181 2154
Size: 9 1172 973
Fragments arranged by size:
1172 973 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 1587 1619 2154
Size: 218 32 1337 32 535
Fragments arranged by size:
1337 535 218 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 838 2154
Size: 838 1316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 815 2154
Size: 417 398 1339
Fragments arranged by size:
1339 417 398
XmaI C'CCGG_G
Cuts at: 0 1211 2154
Size: 1211 943
XmnI GAAnn'nnTTC
Cuts at: 0 773 954 1619 2154
Size: 773 181 665 535
Fragments arranged by size:
773 665 535 181
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AvaI BaeI BanI
BbsI BbvCI BciVI BclI BglI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstYI Bsu36I BtsI CspCI DraI
EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
MfeI MmeI MscI MslI NspI PciI PflMI PfoI
PpuMI PsrI PstI SexAI SfcI SmaI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BanII BbeI BcgI BfrBI BglII BlpI
BmgBI BmtI BpuEI BsaAI BsaHI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstXI BstZ17I BtgI
BtgZI ClaI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI
XbaI XhoI ZraI