Restriction map for
pFN21AB6428
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaLI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoT22I
FokI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I MaeI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI SwaI TspRI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 453 GTAC GT^AC
Aor51HI 810 AGCGCT AGC^GCT
ApaI 391 GGGCCC GGGCC^C
AscI 207 GGCGCGCC GG^CGCGCC
AvaII 91 GGACC G^G[AT]CC
BanII 391 GGGCCC G[GA]GC[CT]^C
BbvCI 239 CCTCAGC CCTCAGC(-5/-2)
BclI 155 TGATCA T^GATCA
BcnI 164 CCGGG CC^[GC]GG
BglII 603 AGATCT A^GATCT
BmyI 391 GGGCCC G[AGT]GC[ACT]^C
BpmI 860 CTGGAG CTGGAG(16/14)
BseRI 185 GAGGAG GAGGAG(10/8)
BsmAI 863 GAGAC GTCTC(1/5)
BsmFI 468 GGGAC GGGAC(10/14)
Bsp1286I 391 GGGCCC G[AGT]GC[ACT]^C
BssHII 208 GCGCGC G^CGCGC
BstYI 603 AGATCT [GA]^GATC[CT]
DraII 391 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 583 CCGCGG C^C[GA][CT]GG
Eco47III 810 AGCGCT AGC^GCT
EcoNI 395 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 391 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 64 GAATTC G^AATTC
EcoRV 547 GATATC GAT^ATC
FauI 194 GCGGG CCCGC(4/6)
FbaI 155 TGATCA T^GATCA
HaeII 810 AGCGCT [GA]GCGC^[CT]
HapII 164 CCGG C^CGG
HindIII 335 AAGCTT A^AGCTT
HpaII 164 CCGG C^CGG
KspI 583 CCGCGG CCGC^GG
MflI 603 AGATCT [GA]^GATC[CT]
MseI 58 TTAA T^TAA
MslI 798 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 164 CCGG C^CGG
NciI 164 CCGGG CC^[GC]GG
NlaIII 963 CATG CATG^
PleI 470 GACTC GAGTC(4/5)
PvuII 760 CAGCTG CAG^CTG
RsaI 453 GTAC GT^AC
SacII 583 CCGCGG CCGC^GG
SfcI 329 CTGTAG C^T[GA][CT]AG
SmlI 593 CTCAAG C^T[CT][GA]AG
SnaBI 955 TACGTA TAC^GTA
SspI 54 AATATT AAT^ATT
StuI 135 AGGCCT AGG^CCT
TaqI 924 TCGA T^CGA
Tru9I 58 TTAA T^TAA
TthHB8I 924 TCGA T^CGA
XhoII 603 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 209 CGCG CG^CG
584 CGCG
BbsI 445 GTCTTC GAAGAC(2/6)
696 GTCTTC
BpuAI 445 GTCTTC GAAGAC(2/6)
696 GTCTTC
BsgI 233 CTGCAC GTGCAG(16/14)
749 CTGCAC
BsiYI 362 CCCTCGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
395 CCTTGGGAAGG
BslI 362 CCCTCGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
395 CCTTGGGAAGG
BsrI 934 ACTGG ACTGG(1/-1)
754 CCAGT
BstNI 171 CCTGG CC^[AT]GG
838 CCTGG
BstUI 209 CGCG CG^CG
584 CGCG
DdeI 240 CTCAG C^T[ACGT]AG
427 CTGAG
EcoRII 171 CCTGG ^CC[AT]GG
838 CCTGG
EcoT14I 261 CCTTGG C^C[AT][AT]GG
395 CCTTGG
HphI 174 GGTGA GGTGA(8/7)
303 GGTGA
MvaI 171 CCTGG CC^[AT]GG
838 CCTGG
MvnI 209 CGCG CG^CG
584 CGCG
NlaIV 167 GGTTCC GG[ACGT]^[ACGT]CC
391 GGGCCC
StyI 261 CCTTGG C^C[AT][AT]GG
395 CCTTGG
Tsp45I 175 GTGAC ^GT[GC]AC
805 GTGAC
XcmI 217 CCAGATGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
759 CCAGCTGAACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaAI BsaJI BssKI Cac8I CfoI
Cfr13I DpnII Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MwoI
NdeII Sau3AI Sau96I ScrFI TfiI
TseI Tsp509I