Restriction map for
pFN21AB6428
(Linear) (Six-base) MAPSORT of: KIBB6428.seq Check: 9038 from: 1 to: 969
KIBB6428.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AfeI AGC'GCT
Cuts at: 0 812 969
Size: 812 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 764 969
Size: 764 205
ApaI G_GGCC'C
Cuts at: 0 395 969
Size: 395 574
ApoI r'AATT_y
Cuts at: 0 64 532 614 849 969
Size: 64 468 82 235 120
Fragments arranged by size:
468 235 120 82 64
AscI GG'CGCG_CC
Cuts at: 0 208 969
Size: 208 761
BanII G_rGCy'C
Cuts at: 0 395 969
Size: 395 574
BbsI GAAGACnn'nnnn_
Cuts at: 0 438 689 969
Size: 438 251 280
Fragments arranged by size:
438 280 251
BbvCI CC'TCA_GC
Cuts at: 0 240 969
Size: 240 729
BclI T'GATC_A
Cuts at: 0 155 969
Size: 155 814
BglII A'GATC_T
Cuts at: 0 603 969
Size: 603 366
Bme1580I G_kGCm'C
Cuts at: 0 395 969
Size: 395 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 969
Size: 881 88
Bpu10I CC'TnA_GC
Cuts at: 0 240 969
Size: 240 729
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 969
Size: 578 391
BsaAI yAC'GTr
Cuts at: 0 117 452 957 969
Size: 117 335 505 12
Fragments arranged by size:
505 335 117 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 969
Size: 200 769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 734 969
Size: 218 516 235
Fragments arranged by size:
516 235 218
Bsp1286I G_dGCh'C
Cuts at: 0 395 969
Size: 395 574
BssHII G'CGCG_C
Cuts at: 0 208 969
Size: 208 761
BstYI r'GATC_y
Cuts at: 0 603 969
Size: 603 366
BtgI C'CryG_G
Cuts at: 0 583 969
Size: 583 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 365 969
Size: 365 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 969
Size: 881 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 399 969
Size: 399 570
EcoO109I rG'GnC_Cy
Cuts at: 0 392 969
Size: 392 577
EcoRI G'AATT_C
Cuts at: 0 64 969
Size: 64 905
EcoRV GAT'ATC
Cuts at: 0 549 969
Size: 549 420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 937 969
Size: 937 32
HaeII r_GCGC'y
Cuts at: 0 814 969
Size: 814 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 644 676 854 886 969
Size: 644 32 178 32 83
Fragments arranged by size:
644 178 83 32 32
HindIII A'AGCT_T
Cuts at: 0 335 969
Size: 335 634
MslI CAynn'nnrTG
Cuts at: 0 802 969
Size: 802 167
MspA1I CmG'CkG
Cuts at: 0 194 439 585 762 969
Size: 194 245 146 177 207
Fragments arranged by size:
245 207 194 177 146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 969
Size: 757 32 180
Fragments arranged by size:
757 180 32
PspOMI G'GGCC_C
Cuts at: 0 391 969
Size: 391 578
PvuII CAG'CTG
Cuts at: 0 762 969
Size: 762 207
SacII CC_GC'GG
Cuts at: 0 586 969
Size: 586 383
SfcI C'TryA_G
Cuts at: 0 329 969
Size: 329 640
SmlI C'TyrA_G
Cuts at: 0 593 969
Size: 593 376
SnaBI TAC'GTA
Cuts at: 0 957 969
Size: 957 12
SspI AAT'ATT
Cuts at: 0 56 969
Size: 56 913
StuI AGG'CCT
Cuts at: 0 137 969
Size: 137 832
StyI C'CwwG_G
Cuts at: 0 261 395 969
Size: 261 134 574
Fragments arranged by size:
574 261 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 224 766 969
Size: 224 542 203
Fragments arranged by size:
542 224 203
Enzymes that do cut:
AfeI AlfI ApaI ApoI AscI BanII BbsI BbvCI
BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaAI BseRI
BsgI Bsp1286I BssHII BstYI BtgI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII MslI
MspA1I PpiI PspOMI PvuII SacII SfcI SmlI SnaBI
SspI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EcoICRI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI