(Circular) (Six-base) MAPSORT of: pFN21AE0520.seq Check: 767 from: 1 to: 5755 pFN21AE0520 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2600 2600 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 3818 5202 5470 1632 Size: 842 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 842 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2167 2879 1166 Size: 1001 712 4042 Fragments arranged by size: 4042 1001 712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2608 1184 Size: 1424 4331 AlwNI CAG_nnn'CTG Cuts at: 2069 2691 3498 5070 2069 Size: 622 807 1572 2754 Fragments arranged by size: 2754 1572 807 622 ApaI G_GGCC'C Cuts at: 2094 2146 2094 Size: 52 5703 ApaLI G'TGCA_C Cuts at: 2222 3812 4968 2222 Size: 1590 1156 3009 Fragments arranged by size: 3009 1590 1156 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2145 3080 1870 Size: 73 153 49 935 4545 Fragments arranged by size: 4545 935 153 73 49 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 3076 4531 618 Size: 325 217 627 514 775 1455 1842 Fragments arranged by size: 1842 1455 775 627 514 325 217 BanII G_rGCy'C Cuts at: 1391 2094 2146 3074 1391 Size: 703 52 928 4072 Fragments arranged by size: 4072 928 703 52 BbeI G_GCGC'C Cuts at: 2305 2305 Size: 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BbvCI CC'TCA_GC Cuts at: 2047 2933 2047 Size: 886 4869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 2456 3005 Size: 107 122 71 631 605 478 306 549 Cuts at: 3005 4366 136 Size: 1361 1525 Fragments arranged by size: 1525 1361 631 605 549 478 306 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2783 3172 2783 Size: 389 5366 Bme1580I G_kGCm'C Cuts at: 1790 2094 2146 2226 2299 2400 2629 2932 3816 Size: 304 52 80 73 101 229 303 884 Cuts at: 3816 4972 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 884 304 303 229 101 80 73 52 BmgBI CAC'GTC Cuts at: 1318 2361 2860 1318 Size: 1043 499 4213 Fragments arranged by size: 4213 1043 499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2562 2741 4415 1239 Size: 1323 179 1674 2579 Fragments arranged by size: 2579 1674 1323 179 Bpu10I CC'TnA_GC Cuts at: 2047 2079 2933 2047 Size: 32 854 4869 Fragments arranged by size: 4869 854 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2591 3880 4745 5043 2591 Size: 1289 865 298 3303 Fragments arranged by size: 3303 1289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2941 3546 493 Size: 711 1737 605 2702 Fragments arranged by size: 2702 1737 711 605 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2302 3562 3944 4579 Size: 53 83 186 936 769 1260 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1260 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2550 4188 4586 4860 5007 5418 2550 Size: 1638 398 274 147 411 2887 Fragments arranged by size: 2887 1638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 1186 Size: 1468 4287 BseYI C'CCAG_C Cuts at: 2087 2467 2684 3041 4958 5454 2087 Size: 380 217 357 1917 496 2388 Fragments arranged by size: 2388 1917 496 380 357 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2727 1163 Size: 573 507 484 4191 Fragments arranged by size: 4191 573 507 484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2080 2226 3816 3901 4972 2028 Size: 52 146 1590 85 1071 2811 Fragments arranged by size: 2811 1590 1071 146 85 52 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2080 2094 2146 2226 2299 2400 Size: 399 238 52 14 52 80 73 101 Cuts at: 2400 2629 2932 3074 3816 3901 4972 1391 Size: 229 303 142 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 238 229 142 101 85 80 73 52 52 14 BspEI T'CCGG_A Cuts at: 2550 5418 2550 Size: 2868 2887 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2600 3110 844 Size: 1756 510 3489 Fragments arranged by size: 3489 1756 510 BsrBI CCG'CTC Cuts at: 1394 1823 2512 3642 1394 Size: 429 689 1130 3507 Fragments arranged by size: 3507 1130 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2564 4399 4586 1700 Size: 110 754 1835 187 2869 Fragments arranged by size: 2869 1835 754 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2325 794 Size: 1531 4224 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 2656 1184 Size: 1472 4283 BstYI r'GATC_y Cuts at: 1972 2211 3085 3452 3837 3854 5306 5392 5404 Size: 239 874 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 874 385 367 346 239 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2118 2387 2118 Size: 269 5486 BtgI C'CryG_G Cuts at: 513 1065 2154 2364 2502 2599 2877 513 Size: 552 1089 210 138 97 278 3391 Fragments arranged by size: 3391 1089 552 278 210 138 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2932 487 Size: 683 56 1706 3310 Fragments arranged by size: 3310 1706 683 56 BtsI GCAGTG_nn' Cuts at: 2745 3271 4065 4085 2745 Size: 526 794 20 4415 Fragments arranged by size: 4415 794 526 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2151 2496 Size: 54 1425 50 275 18 307 14 345 Cuts at: 2496 2814 3202 4091 5720 8 Size: 318 388 889 1629 43 Fragments arranged by size: 1629 1425 889 388 345 318 307 275 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3043 4342 4716 4862 387 Size: 1064 138 1454 1299 374 146 1280 Fragments arranged by size: 1454 1299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2562 2741 3818 4415 5202 5470 Size: 393 842 88 179 1077 597 787 268 Cuts at: 5470 1239 Size: 1524 Fragments arranged by size: 1524 1077 842 787 597 393 268 179 88 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2633 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2091 2122 2091 Size: 31 5724 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2305 2462 4902 1678 Size: 627 157 2440 2531 Fragments arranged by size: 2531 2440 627 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373 Size: 32 135 32 2824 32 42 32 2626 Fragments arranged by size: 2824 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2301 2301 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2153 2816 10 Size: 54 1425 343 321 663 2949 Fragments arranged by size: 2949 1425 663 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 2031 2167 2267 2363 2879 3713 4072 Size: 648 865 136 100 96 516 834 359 Cuts at: 4072 4231 518 Size: 159 2042 Fragments arranged by size: 2042 865 834 648 516 359 159 136 100 96 MspA1I CmG'CkG Cuts at: 1606 2013 2069 2601 2787 3171 3848 4996 5241 Size: 407 56 532 186 384 677 1148 245 Cuts at: 5241 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 532 407 384 245 186 56 NarI GG'CG_CC Cuts at: 2302 2302 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 2154 2364 2502 513 Size: 552 1089 210 138 3766 Fragments arranged by size: 3766 1089 552 210 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2194 2266 2286 3113 4658 1132 Size: 97 965 72 20 827 1545 2229 Fragments arranged by size: 2229 1545 965 827 97 72 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2863 1108 Size: 1755 4000 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2608 1613 Size: 995 4760 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2090 2142 2090 Size: 52 5703 PstI C_TGCA'G Cuts at: 830 2534 2637 3107 830 Size: 1704 103 470 3478 Fragments arranged by size: 3478 1704 470 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2069 2787 2069 Size: 718 5037 SacII CC_GC'GG Cuts at: 2602 2602 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2530 2633 3103 4238 4919 5110 826 Size: 219 1485 103 470 1135 681 191 1471 Fragments arranged by size: 1485 1471 1135 681 470 219 191 103 SfiI GGCCn_nnn'nGGCC Cuts at: 2150 2150 Size: 5755 SfoI GGC'GCC Cuts at: 2303 2303 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2606 3859 4760 5022 820 Size: 197 926 663 1253 901 262 1553 Fragments arranged by size: 1553 1253 926 901 663 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2286 3113 1132 Size: 1154 827 3774 Fragments arranged by size: 3774 1154 827 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2154 2364 2502 3194 513 Size: 552 1089 210 138 692 3074 Fragments arranged by size: 3074 1089 692 552 210 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI