Restriction map for
pFN21AE0364
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BcgI BciVI BclI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HinfI KasI KpnI MamI MfeI
MluI MroI MslI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 1291 GTCTAC GT^[AC][GT]AC
AcyI 1897 GACGCC G[GA]^CG[CT]C
Aor51HI 1262 AGCGCT AGC^GCT
AviII 1559 TGCGCA TGC^GCA
BanI 177 GGTGCC G^G[CT][GA]CC
BbrPI 1012 CACGTG CAC^GTG
BbsI 1932 GAAGAC GAAGAC(2/6)
BbvCI 753 CCTCAGC CCTCAGC(-5/-2)
BglII 1050 AGATCT A^GATCT
BpuAI 1932 GAAGAC GAAGAC(2/6)
BsaAI 1012 CACGTG [CT]AC^GT[GA]
BsaHI 1897 GACGCC G[GA]^CG[CT]C
BsaI 1249 GAGACC GGTCTC(1/5)
BsaWI 1193 ACCGGA [AT]^CCGG[AT]
BsiEI 1644 CGACCG CG[GA][CT]^CG
BsmAI 1249 GAGAC GTCTC(1/5)
BsmI 1376 GAATGC GAATGC(1/-1)
BspLU11I 121 ACATGT A^CATGT
BstAPI 642 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 598 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 871 CTCTTC CTCTTC(1/4)
Eco47III 1262 AGCGCT AGC^GCT
EcoNI 769 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1559 TGCGCA TGC^GCA
HgaI 1897 GACGC GACGC(5/10)
Hin1I 1897 GACGCC G[GA]^CG[CT]C
HpaI 1738 GTTAAC GTT^AAC
Ksp632I 871 CTCTTC CTCTTC(1/4)
KspI 1524 CCGCGG CCGC^GG
MseI 1739 TTAA T^TAA
NheI 1800 GCTAGC G^CTAGC
NspI 121 ACATGT [GA]CATG^[CT]
PflMI 1011 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1012 CACGTG CAC^GTG
PmlI 1012 CACGTG CAC^GTG
SacII 1524 CCGCGG CCGC^GG
SmlI 321 CTTGAG C^T[CT][GA]AG
Tru9I 1739 TTAA T^TAA
Van91I 1011 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 77 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
824 CAGTGCCTG
BamHI 114 GGATCC G^GATCC
1034 GGATCC
BfaI 1465 CTAG C^TAG
1801 CTAG
BpmI 703 CTGGAG CTGGAG(16/14)
2061 CTGGAG
BseRI 1369 CTCCTC GAGGAG(10/8)
706 GAGGAG
BsgI 1399 GTGCAG GTGCAG(16/14)
1795 GTGCAG
BspMI 836 ACCTGC ACCTGC(4/8)
1716 GCAGGT
BsrBI 361 GAGCGG CCGCTC(-3/-3)
928 GAGCGG
BsrDI 1216 GCAATG GCAATG(2/0)
1223 GCAATG
BssSI 371 CACGAG CACGAG(-5/-1)
1693 CACGAG
BstEII 490 GGTGACC G^GT[ACGT]ACC
1908 GGTCACC
BstPI 490 GGTGACC G^GT[ACGT]ACC
1908 GGTCACC
EcoO65I 490 GGTGACC G^GT[ACGT]ACC
1908 GGTCACC
MaeI 1465 CTAG C^TAG
1801 CTAG
PstI 724 CTGCAG CTGCA^G
937 CTGCAG
PvuII 200 CAGCTG CAG^CTG
974 CAGCTG
SacI 654 GAGCTC GAGCT^C
1150 GAGCTC
SmaI 449 CCCGGG CCC^GGG
665 CCCGGG
XmaCI 449 CCCGGG C^CCGGG
665 CCCGGG
XmaI 449 CCCGGG C^CCGGG
665 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
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AccII AciI AfaI AflIII AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanII BbvI BcnI BglI
BlpI BmyI Bpu1102I BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII