Restriction map for
pFN21AE0364
(Linear) (Six-base) MAPSORT of: KIEE0364.seq Check: 6887 from: 1 to: 2070
KIEE0364.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1707 2070
Size: 1707 363
AccI GT'mk_AC
Cuts at: 0 1292 2070
Size: 1292 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 496 2070
Size: 325 171 1574
Fragments arranged by size:
1574 325 171
AfeI AGC'GCT
Cuts at: 0 1264 2070
Size: 1264 806
AflIII A'CryG_T
Cuts at: 0 121 1682 1742 2070
Size: 121 1561 60 328
Fragments arranged by size:
1561 328 121 60
AlwNI CAG_nnn'CTG
Cuts at: 0 82 829 2070
Size: 82 747 1241
Fragments arranged by size:
1241 747 82
ApaI G_GGCC'C
Cuts at: 0 474 666 1350 2007 2070
Size: 474 192 684 657 63
Fragments arranged by size:
684 657 474 192 63
AvaI C'yCGr_G
Cuts at: 0 449 665 885 1532 1651 2070
Size: 449 216 220 647 119 419
Fragments arranged by size:
647 449 419 220 216 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1152 1185 2070
Size: 1152 33 885
Fragments arranged by size:
1152 885 33
BamHI G'GATC_C
Cuts at: 0 114 1034 2070
Size: 114 920 1036
Fragments arranged by size:
1036 920 114
BanI G'GyrC_C
Cuts at: 0 177 2070
Size: 177 1893
BanII G_rGCy'C
Cuts at: 0 474 658 666 789 1154 1350 2007 2070
Size: 474 184 8 123 365 196 657 63
Fragments arranged by size:
657 474 365 196 184 123 63 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1939 2070
Size: 1939 131
BbvCI CC'TCA_GC
Cuts at: 0 754 2070
Size: 754 1316
BglI GCCn_nnn'nGGC
Cuts at: 0 72 186 234 460 478 2070
Size: 72 114 48 226 18 1592
Fragments arranged by size:
1592 226 114 72 48 18
BglII A'GATC_T
Cuts at: 0 1050 2070
Size: 1050 1020
BlpI GC'TnA_GC
Cuts at: 0 190 482 652 2070
Size: 190 292 170 1418
Fragments arranged by size:
1418 292 190 170
Bme1580I G_kGCm'C
Cuts at: 0 182 393 474 666 1350 2007 2070
Size: 182 211 81 192 684 657 63
Fragments arranged by size:
684 657 211 192 182 81 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 817 2001 2070
Size: 817 1184 69
Fragments arranged by size:
1184 817 69
BmtI G_CTAG'C
Cuts at: 0 1804 2070
Size: 1804 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 2070
Size: 724 1346
Bpu10I CC'TnA_GC
Cuts at: 0 754 2070
Size: 754 1316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 2070
Size: 342 1728
BsaI GGTCTCn'nnnn_
Cuts at: 0 1243 2070
Size: 1243 827
BsaAI yAC'GTr
Cuts at: 0 1014 2070
Size: 1014 1056
BsaHI Gr'CG_yC
Cuts at: 0 1898 2070
Size: 1898 172
BsaWI w'CCGG_w
Cuts at: 0 1193 2070
Size: 1193 877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 206 236 1693 1723 1819 1849 2070
Size: 206 30 1457 30 96 30 221
Fragments arranged by size:
1457 221 206 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 1360 2070
Size: 721 639 710
Fragments arranged by size:
721 710 639
BseYI C'CCAG_C
Cuts at: 0 486 573 588 844 1178 2070
Size: 486 87 15 256 334 892
Fragments arranged by size:
892 486 334 256 87 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1816 2070
Size: 1420 396 254
Fragments arranged by size:
1420 396 254
BsiEI CG_ry'CG
Cuts at: 0 1647 2070
Size: 1647 423
BsiHKAI G_wGCw'C
Cuts at: 0 372 658 1154 2070
Size: 372 286 496 916
Fragments arranged by size:
916 496 372 286
BsmI GAATG_Cn'
Cuts at: 0 1382 2070
Size: 1382 688
Bsp1286I G_dGCh'C
Cuts at: 0 182 372 393 474 658 666 789 1154
Size: 182 190 21 81 184 8 123 365
Cuts at: 1154 1350 2007 2070
Size: 196 657 63
Fragments arranged by size:
657 365 196 190 184 182 123 81
63 21 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 1707 2070
Size: 845 862 363
Fragments arranged by size:
862 845 363
BsrBI CCG'CTC
Cuts at: 0 363 930 2070
Size: 363 567 1140
Fragments arranged by size:
1140 567 363
BsrDI GCAATG_nn'
Cuts at: 0 1223 1230 2070
Size: 1223 7 840
Fragments arranged by size:
1223 840 7
BsrFI r'CCGG_y
Cuts at: 0 261 326 418 2070
Size: 261 65 92 1652
Fragments arranged by size:
1652 261 92 65
BssSI C'ACGA_G
Cuts at: 0 371 1693 2070
Size: 371 1322 377
Fragments arranged by size:
1322 377 371
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 2070
Size: 648 1422
BstEII G'GTnAC_C
Cuts at: 0 490 1908 2070
Size: 490 1418 162
Fragments arranged by size:
1418 490 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 605 2070
Size: 605 1465
BstYI r'GATC_y
Cuts at: 0 114 287 710 1034 1050 1280 2070
Size: 114 173 423 324 16 230 790
Fragments arranged by size:
790 423 324 230 173 114 16
BtgI C'CryG_G
Cuts at: 0 183 1524 1613 1901 2070
Size: 183 1341 89 288 169
Fragments arranged by size:
1341 288 183 169 89
DraIII CAC_nnn'GTG
Cuts at: 0 648 825 1698 2070
Size: 648 177 873 372
Fragments arranged by size:
873 648 372 177
EaeI y'GGCC_r
Cuts at: 0 73 143 281 581 621 1610 1809 2070
Size: 73 70 138 300 40 989 199 261
Fragments arranged by size:
989 300 261 199 138 73 70 40
EarI CTCTTCn'nnn_
Cuts at: 0 877 2070
Size: 877 1193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1198 1435 2070
Size: 1198 237 635
Fragments arranged by size:
1198 635 237
EcoICRI GAG'CTC
Cuts at: 0 656 1152 2070
Size: 656 496 918
Fragments arranged by size:
918 656 496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 496 724 2070
Size: 325 171 228 1346
Fragments arranged by size:
1346 325 228 171
EcoNI CCTnn'n_nnAGG
Cuts at: 0 773 2070
Size: 773 1297
EcoO109I rG'GnC_Cy
Cuts at: 0 54 409 471 662 1346 1347 2070
Size: 54 355 62 191 684 1 723
Fragments arranged by size:
723 684 355 191 62 54 1
FspI TGC'GCA
Cuts at: 0 1561 2070
Size: 1561 509
HaeII r_GCGC'y
Cuts at: 0 814 1266 1341 2070
Size: 814 452 75 729
Fragments arranged by size:
814 729 452 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1422 1454 1669 1701 2070
Size: 1422 32 215 32 369
Fragments arranged by size:
1422 369 215 32 32
HincII GTy'rAC
Cuts at: 0 1326 1740 1977 2070
Size: 1326 414 237 93
Fragments arranged by size:
1326 414 237 93
HpaI GTT'AAC
Cuts at: 0 1740 2070
Size: 1740 330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 2070
Size: 1523 547
MscI TGG'CCA
Cuts at: 0 75 145 283 583 1612 2070
Size: 75 70 138 300 1029 458
Fragments arranged by size:
1029 458 300 138 75 70
MspA1I CmG'CkG
Cuts at: 0 202 743 976 1526 2070
Size: 202 541 233 550 544
Fragments arranged by size:
550 544 541 233 202
NaeI GCC'GGC
Cuts at: 0 263 328 420 2070
Size: 263 65 92 1650
Fragments arranged by size:
1650 263 92 65
NcoI C'CATG_G
Cuts at: 0 183 1613 1901 2070
Size: 183 1430 288 169
Fragments arranged by size:
1430 288 183 169
NgoMIV G'CCGG_C
Cuts at: 0 261 326 418 2070
Size: 261 65 92 1652
Fragments arranged by size:
1652 261 92 65
NheI G'CTAG_C
Cuts at: 0 1800 2070
Size: 1800 270
NspI r_CATG'y
Cuts at: 0 125 2070
Size: 125 1945
PasI CC'CwG_GG
Cuts at: 0 173 1350 2070
Size: 173 1177 720
Fragments arranged by size:
1177 720 173
PciI A'CATG_T
Cuts at: 0 121 2070
Size: 121 1949
PflMI CCAn_nnn'nTGG
Cuts at: 0 1017 2070
Size: 1017 1053
PfoI T'CCnGG_A
Cuts at: 0 692 1892 2070
Size: 692 1200 178
Fragments arranged by size:
1200 692 178
PmlI CAC'GTG
Cuts at: 0 1014 2070
Size: 1014 1056
PspOMI G'GGCC_C
Cuts at: 0 470 662 1346 2003 2070
Size: 470 192 684 657 67
Fragments arranged by size:
684 657 470 192 67
PstI C_TGCA'G
Cuts at: 0 728 941 2070
Size: 728 213 1129
Fragments arranged by size:
1129 728 213
PvuII CAG'CTG
Cuts at: 0 202 976 2070
Size: 202 774 1094
Fragments arranged by size:
1094 774 202
SacI G_AGCT'C
Cuts at: 0 658 1154 2070
Size: 658 496 916
Fragments arranged by size:
916 658 496
SacII CC_GC'GG
Cuts at: 0 1527 2070
Size: 1527 543
SfcI C'TryA_G
Cuts at: 0 724 937 1457 2070
Size: 724 213 520 613
Fragments arranged by size:
724 613 520 213
SmaI CCC'GGG
Cuts at: 0 451 667 2070
Size: 451 216 1403
Fragments arranged by size:
1403 451 216
SmlI C'TyrA_G
Cuts at: 0 321 2070
Size: 321 1749
SrfI GCCC'GGGC
Cuts at: 0 451 2070
Size: 451 1619
StyI C'CwwG_G
Cuts at: 0 183 1490 1613 1901 2070
Size: 183 1307 123 288 169
Fragments arranged by size:
1307 288 183 169 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 478 836 1258 2070
Size: 165 313 358 422 812
Fragments arranged by size:
812 422 358 313 165
TatI w'GTAC_w
Cuts at: 0 1989 2070
Size: 1989 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 1190 1222 2070
Size: 206 32 952 32 848
Fragments arranged by size:
952 848 206 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 251 1068 1695 2070
Size: 251 817 627 375
Fragments arranged by size:
817 627 375 251
XmaI C'CCGG_G
Cuts at: 0 449 665 2070
Size: 449 216 1405
Fragments arranged by size:
1405 449 216
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII HpaI MmeI MscI MspA1I NaeI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmlI PspOMI
PstI PvuII SacI SacII SfcI SmaI SmlI SrfI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BbeI
BcgI BciVI BclI BfrBI BmgBI BplI BsaBI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FalI FseI FspAI HindIII KasI KpnI MfeI
MluI MslI NarI NdeI NotI NruI NsiI PacI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI