(Circular) (Six-base) MAPSORT of: pFN21AE0543.seq Check: 675 from: 1 to: 5650 pFN21AE0543 5650 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 3713 5097 5365 1632 Size: 698 1383 1384 268 1917 Fragments arranged by size: 1917 1384 1383 698 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 2781 4549 5526 1203 Size: 1578 1768 977 1327 Fragments arranged by size: 1768 1578 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 2020 2539 3393 4965 2020 Size: 519 854 1572 2705 Fragments arranged by size: 2705 1572 854 519 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2959 3185 3337 5446 1493 Size: 1466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2172 2975 1870 Size: 73 229 803 4545 Fragments arranged by size: 4545 803 229 73 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2490 2564 2971 4426 618 Size: 325 217 627 703 74 407 1455 1842 Fragments arranged by size: 1842 1455 703 627 407 325 217 74 BanII G_rGCy'C Cuts at: 1391 2064 2432 2550 2649 2969 1391 Size: 673 368 118 99 320 4072 Fragments arranged by size: 4072 673 368 320 118 99 BbeI G_GCGC'C Cuts at: 2494 2494 Size: 5650 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 928 Size: 971 272 4407 Fragments arranged by size: 4407 971 272 BbvCI CC'TCA_GC Cuts at: 2437 2437 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 4261 136 Size: 107 122 71 631 605 985 1604 1525 Fragments arranged by size: 1604 1525 985 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2869 3711 4867 1790 Size: 1079 842 1156 2573 Fragments arranged by size: 2573 1156 1079 842 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 2437 4310 1239 Size: 978 220 1873 2579 Fragments arranged by size: 2579 1873 978 220 Bpu10I CC'TnA_GC Cuts at: 2437 2437 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 2744 2883 3775 4640 4938 2366 Size: 378 139 892 865 298 3078 Fragments arranged by size: 3078 892 865 378 298 139 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2407 2491 2660 2723 Size: 53 83 186 936 874 84 169 63 Cuts at: 2723 3457 3839 4474 275 Size: 734 382 635 1451 Fragments arranged by size: 1451 936 874 734 635 382 186 169 84 83 63 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 1186 Size: 1378 4272 BseYI C'CCAG_C Cuts at: 2339 2543 4853 5349 2339 Size: 204 2310 496 2640 Fragments arranged by size: 2640 2310 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2715 3100 3861 4889 1540 Size: 275 186 714 385 761 1028 2301 Fragments arranged by size: 2301 1028 761 714 385 275 186 BsiHKAI G_wGCw'C Cuts at: 2649 3711 3796 4867 2649 Size: 1062 85 1071 3432 Fragments arranged by size: 3432 1071 1062 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2432 2550 2649 2869 2969 3711 Size: 399 274 368 118 99 220 100 742 Cuts at: 3711 3796 4867 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 274 220 118 100 99 85 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2844 3539 5269 2844 Size: 695 1730 3225 Fragments arranged by size: 3225 1730 695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2687 3005 844 Size: 1240 603 318 3489 Fragments arranged by size: 3489 1240 603 318 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2508 4145 4319 65 Size: 1183 1260 1637 174 1396 Fragments arranged by size: 1637 1396 1260 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 4294 4481 1700 Size: 110 841 1643 187 2869 Fragments arranged by size: 2869 1643 841 187 110 BsrGI T'GTAC_A Cuts at: 96 2136 2784 96 Size: 2040 648 2962 Fragments arranged by size: 2962 2040 648 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 2063 3710 4722 2063 Size: 1647 1012 2991 Fragments arranged by size: 2991 1647 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 2071 2359 1184 Size: 887 288 4475 Fragments arranged by size: 4475 887 288 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2251 2095 Size: 156 5494 BstYI r'GATC_y Cuts at: 1972 2017 2980 3347 3732 3749 5201 5287 5299 Size: 45 963 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 513 Size: 552 1305 3793 Fragments arranged by size: 3793 1305 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 2217 2724 3166 3960 3980 2217 Size: 507 442 794 20 3887 Fragments arranged by size: 3887 794 507 442 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2491 2526 529 Size: 35 1927 35 3653 Fragments arranged by size: 3653 1927 35 35 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2256 2412 Size: 54 1425 50 275 18 267 159 156 Cuts at: 2412 3097 3986 5615 8 Size: 685 889 1629 43 Fragments arranged by size: 1629 1425 889 685 275 267 159 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 4237 4611 4757 387 Size: 1064 138 603 2045 374 146 1280 Fragments arranged by size: 2045 1280 1064 603 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 2647 Size: 5650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2330 2437 3713 4310 5097 5365 Size: 393 585 113 107 1276 597 787 268 Cuts at: 5365 1239 Size: 1524 Fragments arranged by size: 1524 1276 787 597 585 393 268 113 107 EcoNI CCTnn'n_nnAGG Cuts at: 2774 2774 Size: 5650 EcoO109I rG'GnC_Cy Cuts at: 2457 2536 2557 2457 Size: 79 21 5550 Fragments arranged by size: 5550 79 21 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 5650 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 2494 2856 4797 1678 Size: 816 362 1941 2531 Fragments arranged by size: 2531 1941 816 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2819 2851 2916 2948 4291 Size: 32 135 32 1247 32 65 32 1343 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1343 1247 135 65 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KasI G'GCGC_C Cuts at: 2490 2490 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4764 4948 1305 Size: 3459 184 2007 Fragments arranged by size: 3459 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 3561 Fragments arranged by size: 3561 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 3608 3967 4126 518 Size: 648 1209 1233 359 159 2042 Fragments arranged by size: 2042 1233 1209 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2302 2392 3066 3743 4891 5136 1606 Size: 696 90 674 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 674 245 90 NarI GG'CG_CC Cuts at: 2491 2491 Size: 5650 NcoI C'CATG_G Cuts at: 513 1065 2370 513 Size: 552 1305 3793 Fragments arranged by size: 3793 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2197 2785 3008 4553 1132 Size: 97 968 588 223 1545 2229 Fragments arranged by size: 2229 1545 968 588 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 2781 4549 2781 Size: 1768 3882 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 1108 Size: 1248 4402 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2457 2457 Size: 5650 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2115 2212 2397 3002 830 Size: 1285 97 185 605 3478 Fragments arranged by size: 3478 1285 605 185 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2392 2392 Size: 5650 SacI G_AGCT'C Cuts at: 2649 2649 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2111 2208 2393 2551 2998 4133 4814 Size: 219 1066 97 185 158 447 1135 681 Cuts at: 4814 5005 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1066 681 447 219 191 185 158 97 SfoI GGC'GCC Cuts at: 2492 2492 Size: 5650 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2381 2759 2898 3754 4655 4917 Size: 197 926 438 378 139 856 901 262 Cuts at: 4917 820 Size: 1553 Fragments arranged by size: 1553 926 901 856 438 378 262 197 139 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2769 1517 Size: 1252 4398 StyI C'CwwG_G Cuts at: 513 1065 2370 3089 513 Size: 552 1305 719 3074 Fragments arranged by size: 3074 1305 719 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 2552 3677 3694 3847 4032 4477 Size: 1255 918 54 1125 17 153 185 445 Cuts at: 4477 325 Size: 1498 Fragments arranged by size: 1498 1255 1125 918 445 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2784 3896 Size: 274 80 33 51 1443 159 648 1112 Cuts at: 3896 5608 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1112 648 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2728 2986 2728 Size: 258 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI