(Circular) (Six-base) MAPSORT of: pFN21AB7254.seq Check: 5776 from: 1 to: 6637 pFN21AB7254 6637 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2200 2200 Size: 6637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3534 4447 278 Size: 53 83 186 936 1998 913 2468 Fragments arranged by size: 2468 1998 936 913 186 83 53 AccI GT'mk_AC Cuts at: 1937 2940 3980 1937 Size: 1003 1040 4594 Fragments arranged by size: 4594 1040 1003 Acc65I G'GTAC_C Cuts at: 3958 3958 Size: 6637 AclI AA'CG_TT Cuts at: 1563 4764 5137 1563 Size: 3201 373 3063 Fragments arranged by size: 3201 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4700 6084 6352 1632 Size: 3068 1384 268 1917 Fragments arranged by size: 3068 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2897 820 Size: 197 1880 4560 Fragments arranged by size: 4560 1880 197 AflIII A'CryG_T Cuts at: 1203 2361 3651 3897 5536 6513 1203 Size: 1158 1290 246 1639 977 1327 Fragments arranged by size: 1639 1327 1290 1158 977 246 AgeI A'CCGG_T Cuts at: 5468 5468 Size: 6637 AhdI GACnn_n'nnGTC Cuts at: 5366 5366 Size: 6637 AleI CACnn'nnGTG Cuts at: 1166 2439 2552 3908 1166 Size: 1273 113 1356 3895 Fragments arranged by size: 3895 1356 1273 113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6637 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3413 3445 3413 Size: 32 6605 AlwNI CAG_nnn'CTG Cuts at: 2874 3700 4380 5952 2874 Size: 826 680 1572 3559 Fragments arranged by size: 3559 1572 826 680 ApaI G_GGCC'C Cuts at: 3165 3165 Size: 6637 ApaLI G'TGCA_C Cuts at: 4694 5850 4694 Size: 1156 5481 ApoI r'AATT_y Cuts at: 1493 2285 2908 3946 4172 4324 6433 1493 Size: 792 623 1038 226 152 2109 1697 Fragments arranged by size: 2109 1697 1038 792 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 5191 160 Size: 872 4159 1606 Fragments arranged by size: 4159 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 3328 3962 1870 Size: 73 1385 634 4545 Fragments arranged by size: 4545 1385 634 73 BamHI G'GATC_C Cuts at: 3716 3967 4334 3716 Size: 251 367 6019 Fragments arranged by size: 6019 367 251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2637 2816 3843 3958 Size: 325 217 627 759 91 179 1027 115 Cuts at: 3958 5413 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1027 759 627 325 217 179 115 91 BanII G_rGCy'C Cuts at: 1391 2561 2604 3165 3691 3956 1391 Size: 1170 43 561 526 265 4072 Fragments arranged by size: 4072 1170 561 526 265 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2326 2509 928 Size: 971 282 145 183 5056 Fragments arranged by size: 5056 971 282 183 145 BbvCI CC'TCA_GC Cuts at: 2645 3219 2645 Size: 574 6063 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4828 4862 1903 Size: 34 50 34 2807 34 3678 Fragments arranged by size: 3678 2807 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4529 5739 4529 Size: 1210 5427 BclI T'GATC_A Cuts at: 1548 2931 3057 1548 Size: 1383 126 5128 Fragments arranged by size: 5128 1383 126 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 5248 136 Size: 107 122 71 631 605 430 3146 1525 Fragments arranged by size: 3146 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 2418 6632 2418 Size: 4214 2423 BlpI GC'TnA_GC Cuts at: 2075 2560 4054 2075 Size: 485 1494 4658 Fragments arranged by size: 4658 1494 485 Bme1580I G_kGCm'C Cuts at: 1790 3165 3175 4698 5854 1790 Size: 1375 10 1523 1156 2573 Fragments arranged by size: 2573 1523 1375 1156 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4368 5326 442 Size: 416 53 83 399 24 2951 958 1753 Fragments arranged by size: 2951 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5297 1239 Size: 4058 2579 Bpu10I CC'TnA_GC Cuts at: 2645 3219 2645 Size: 574 6063 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4762 5627 5925 4762 Size: 865 298 5474 Fragments arranged by size: 5474 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3683 5300 882 Size: 617 2184 1617 2219 Fragments arranged by size: 2219 2184 1617 617 BsaAI yAC'GTr Cuts at: 493 1204 4428 493 Size: 711 3224 2702 Fragments arranged by size: 3224 2702 711 BsaBI GATnn'nnATC Cuts at: 6631 6631 Size: 6637 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3531 3798 4444 4826 Size: 53 83 186 936 1998 267 646 382 Cuts at: 4826 5461 275 Size: 635 1451 Fragments arranged by size: 1998 1451 936 646 635 382 267 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 5070 5468 5742 5889 6300 2247 Size: 2823 398 274 147 411 2584 Fragments arranged by size: 2823 2584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3413 3443 1178 Size: 30 165 30 2010 30 4372 Fragments arranged by size: 4372 2010 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2818 3284 3293 3839 1186 Size: 1632 466 9 546 3984 Fragments arranged by size: 3984 1632 546 466 9 BseYI C'CCAG_C Cuts at: 2453 2609 3164 3417 3560 3832 5840 6336 2453 Size: 156 555 253 143 272 2008 496 2754 Fragments arranged by size: 2754 2008 555 496 272 253 156 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2248 2417 1163 Size: 573 512 169 5383 Fragments arranged by size: 5383 573 512 169 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2012 4087 4848 5876 1540 Size: 275 186 11 2075 761 1028 2301 Fragments arranged by size: 2301 2075 1028 761 275 186 11 BsiHKAI G_wGCw'C Cuts at: 2349 4698 4783 5854 2349 Size: 2349 85 1071 3132 Fragments arranged by size: 3132 2349 1071 85 BsmI GAATG_Cn' Cuts at: 4148 4241 4148 Size: 93 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2561 2604 3165 3175 3691 3956 Size: 399 559 212 43 561 10 516 265 Cuts at: 3956 4698 4783 5854 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 559 516 399 265 212 85 43 10 BspEI T'CCGG_A Cuts at: 2247 6300 2247 Size: 4053 2584 BspHI T'CATG_A Cuts at: 2766 3000 3882 4526 6256 2766 Size: 234 882 644 1730 3147 Fragments arranged by size: 3147 1730 882 644 234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 2605 3924 3992 844 Size: 1356 405 1319 68 3489 Fragments arranged by size: 3489 1356 1319 405 68 BsrBI CCG'CTC Cuts at: 1394 1823 4524 1394 Size: 429 2701 3507 Fragments arranged by size: 3507 2701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5132 5306 65 Size: 1183 3884 174 1396 Fragments arranged by size: 3884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5281 5468 1700 Size: 110 3471 187 2869 Fragments arranged by size: 3471 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6637 BssHII G'CGCG_C Cuts at: 1925 2031 1925 Size: 106 6531 BssSI C'ACGA_G Cuts at: 4697 5709 4697 Size: 1012 5625 BstAPI GCAn_nnn'nTGC Cuts at: 794 2262 2874 794 Size: 1468 612 4557 Fragments arranged by size: 4557 1468 612 BstBI TT'CG_AA Cuts at: 2995 5441 2995 Size: 2446 4191 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6637 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2467 Size: 6637 BstYI r'GATC_y Cuts at: 1972 2418 3716 3967 4334 4719 4736 6188 6274 Size: 446 1298 251 367 385 17 1452 86 Cuts at: 6274 6286 6632 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1298 446 385 367 346 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3039 3144 513 Size: 552 1974 105 4006 Fragments arranged by size: 4006 1974 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2547 487 Size: 683 56 1321 4577 Fragments arranged by size: 4577 1321 683 56 BtsI GCAGTG_nn' Cuts at: 3175 4153 4947 4967 3175 Size: 978 794 20 4845 Fragments arranged by size: 4845 978 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6602 DraI TTT'AAA Cuts at: 3941 3941 Size: 6637 DraIII CAC_nnn'GTG Cuts at: 3145 3459 3145 Size: 314 6323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2399 5644 809 Size: 507 1083 3245 1802 Fragments arranged by size: 3245 1802 1083 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2009 2376 2841 Size: 54 1425 50 275 18 179 367 465 Cuts at: 2841 3042 3117 3365 3585 3600 4084 4973 6602 Size: 201 75 248 220 15 484 889 1629 Cuts at: 6602 8 Size: 43 Fragments arranged by size: 1629 1425 889 484 465 367 275 248 220 201 179 75 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2009 4084 1537 Size: 275 197 2075 4090 Fragments arranged by size: 4090 2075 275 197 EarI CTCTTCn'nnn_ Cuts at: 1353 3270 4567 1353 Size: 1917 1297 3423 Fragments arranged by size: 3423 1917 1297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5224 5598 5744 387 Size: 1064 138 3635 374 146 1280 Fragments arranged by size: 3635 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4700 5297 6084 6352 1239 Size: 393 3068 597 787 268 1524 Fragments arranged by size: 3068 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2065 3161 2065 Size: 1096 5541 EcoRI G'AATT_C Cuts at: 2285 3946 4324 2285 Size: 1661 378 4598 Fragments arranged by size: 4598 1661 378 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 2108 2431 5784 1678 Size: 430 323 3353 2531 Fragments arranged by size: 3353 2531 430 323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2936 2968 3311 3343 3701 Size: 32 135 32 1364 32 343 32 358 Cuts at: 3701 3708 3733 3740 5278 5310 5352 5384 1373 Size: 7 25 7 1538 32 42 32 2626 Fragments arranged by size: 2626 1538 1364 358 343 135 42 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2833 3981 4227 669 Size: 988 281 895 1148 246 3079 Fragments arranged by size: 3079 1148 988 895 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6637 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KpnI G_GTAC'C Cuts at: 3962 3962 Size: 6637 MfeI C'AATT_G Cuts at: 4236 4236 Size: 6637 MluI A'CGCG_T Cuts at: 6513 6513 Size: 6637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3657 5751 5935 1305 Size: 2352 2094 184 2007 Fragments arranged by size: 2352 2094 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 2843 3044 3119 3587 Size: 54 1425 343 546 465 201 75 468 Cuts at: 3587 3602 10 Size: 15 3045 Fragments arranged by size: 3045 1425 546 468 465 343 201 75 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2439 2465 2552 3485 3626 3908 Size: 648 1194 79 26 87 933 141 282 Cuts at: 3908 4595 4954 5113 518 Size: 687 359 159 2042 Fragments arranged by size: 2042 1194 933 687 648 359 282 159 141 87 79 26 MspA1I CmG'CkG Cuts at: 1606 2474 2564 3370 3820 4053 4730 5878 6123 Size: 868 90 806 450 233 677 1148 245 Cuts at: 6123 1606 Size: 2120 Fragments arranged by size: 2120 1148 868 806 677 450 245 233 90 NcoI C'CATG_G Cuts at: 513 1065 3039 513 Size: 552 1974 4111 Fragments arranged by size: 4111 1974 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 2365 3055 3995 5540 1132 Size: 97 1136 690 940 1545 2229 Fragments arranged by size: 2229 1545 1136 940 690 97 PasI CC'CwG_GG Cuts at: 1348 3517 3684 1348 Size: 2169 167 4301 Fragments arranged by size: 4301 2169 167 PciI A'CATG_T Cuts at: 2361 5536 2361 Size: 3175 3462 PflMI CCAn_nnn'nTGG Cuts at: 1108 3459 1108 Size: 2351 4286 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 4706 4738 6219 6251 3413 Size: 32 1261 32 1481 32 3799 Fragments arranged by size: 3799 1481 1261 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 6637 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 4207 4207 Size: 6637 PspOMI G'GGCC_C Cuts at: 3161 3161 Size: 6637 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3848 3880 3848 Size: 32 6605 PstI C_TGCA'G Cuts at: 830 3696 3989 830 Size: 2866 293 3478 Fragments arranged by size: 3478 2866 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 PvuII CAG'CTG Cuts at: 3820 3820 Size: 6637 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 4885 6597 4885 Size: 1712 4925 SfcI C'TryA_G Cuts at: 826 1045 3692 3985 5120 5801 5992 826 Size: 219 2647 293 1135 681 191 1471 Fragments arranged by size: 2647 1471 1135 681 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6637 SmaI CCC'GGG Cuts at: 1872 3964 1872 Size: 2092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 4741 5642 5904 820 Size: 197 926 954 1844 901 262 1553 Fragments arranged by size: 1844 1553 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4428 493 Size: 3935 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6637 SphI G_CATG'C Cuts at: 1132 3995 1132 Size: 2863 3774 SspI AAT'ATT Cuts at: 5 52 4561 5 Size: 47 4509 2081 Fragments arranged by size: 4509 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6637 StyI C'CwwG_G Cuts at: 513 1065 2409 3039 4076 513 Size: 552 1344 630 1037 3074 Fragments arranged by size: 3074 1344 1037 630 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2494 3665 3831 4664 4681 4834 5019 Size: 1255 914 1171 166 833 17 153 185 Cuts at: 5019 5464 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1171 914 833 445 185 166 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4883 6595 96 Size: 274 80 33 51 1443 2906 1712 138 Fragments arranged by size: 2906 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2800 2832 2957 2989 6597 6629 2800 Size: 32 125 32 3608 32 2808 Fragments arranged by size: 3608 2808 125 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3973 3973 Size: 6637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 3595 3610 2533 Size: 1062 15 5560 Fragments arranged by size: 5560 1062 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6637 XmaI C'CCGG_G Cuts at: 1870 3962 1870 Size: 2092 4545 XmnI GAAnn'nnTTC Cuts at: 3428 4766 3428 Size: 1338 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3532 4445 276 Size: 53 83 186 936 1998 913 2468 Fragments arranged by size: 2468 1998 936 913 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI