Restriction map for
pFN21AB7228
(Circular) (Six-base) MAPSORT of: pFN21AB7228.seq Check: 6175 from: 1 to: 6490
pFN21AB7228 6490 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 2777 3811 2777
Size: 1034 5456
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 2897 4553 5937 6205 1632
Size: 367 898 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 898 367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2711 2723 5389 6366 1203
Size: 1508 12 2666 977 1327
Fragments arranged by size:
2666 1508 1327 977 12
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 3671 5219 3671
Size: 1548 4942
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 4233 5805 4233
Size: 1572 4918
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2009 2311 2637 3281 3799 4025 4177 6286
Size: 516 302 326 644 518 226 152 2109
Cuts at: 6286 1493
Size: 1697
Fragments arranged by size:
2109 1697 644 518 516 326 302 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 3815 1870
Size: 73 1872 4545
Fragments arranged by size:
4545 1872 73
AvrII C'CTAG_G
Cuts at: 2926 2926
Size: 6490
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2777 3811 5266 618
Size: 325 217 627 990 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 990 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3763 3809 1391
Size: 2372 46 4072
Fragments arranged by size:
4072 2372 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5519
BbvCI CC'TCA_GC
Cuts at: 2994 2994
Size: 6490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2609 4382 5592 2609
Size: 1773 1210 3507
Fragments arranged by size:
3507 1773 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 2417 3907 2417
Size: 1490 5000
Bme1580I G_kGCm'C
Cuts at: 1790 4551 5707 1790
Size: 2761 1156 2573
Fragments arranged by size:
2761 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4221 5179 442
Size: 416 53 83 399 24 2804 958 1753
Fragments arranged by size:
2804 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2346 2346
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5150 1239
Size: 3911 2579
Bpu10I CC'TnA_GC
Cuts at: 2994 2994
Size: 6490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3090 3773 5153 882
Size: 617 1591 683 1380 2219
Fragments arranged by size:
2219 1591 1380 683 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 2714 2724 4281 493
Size: 711 1503 7 10 1557 2702
Fragments arranged by size:
2702 1557 1503 711 10 7
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3228 3258 3480 3510 1178
Size: 30 165 30 1825 30 222 30 4158
Fragments arranged by size:
4158 1825 222 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3368 3384 1186
Size: 2182 16 4292
Fragments arranged by size:
4292 2182 16
BseYI C'CCAG_C
Cuts at: 2377 2872 3202 5693 6189 2377
Size: 495 330 2491 496 2678
Fragments arranged by size:
2678 2491 496 495 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 3108 1163
Size: 573 753 619 4545
Fragments arranged by size:
4545 753 619 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4551 4636 5707 4551
Size: 85 1071 5334
Fragments arranged by size:
5334 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 4001 4094 3581
Size: 420 93 5977
Fragments arranged by size:
5977 420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3763 3809 4551 4636 5707 1391
Size: 399 1973 46 742 85 1071 2174
Fragments arranged by size:
2174 1973 1071 742 399 85 46
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 2385 4379 6109 2385
Size: 1994 1730 2766
Fragments arranged by size:
2766 1994 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3845 844
Size: 3001 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 2451 2733 4985 5159 65
Size: 1183 911 292 282 2252 174 1396
Fragments arranged by size:
2252 1396 1183 911 292 282 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2114 794
Size: 1320 5170
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstXI CCAn_nnnn'nTGG
Cuts at: 3023 3023
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2904 3820 4187 4572 4589 6041 6127 6139
Size: 932 916 367 385 17 1452 86 12
Cuts at: 6139 6485 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 932 916 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2439 2539 513
Size: 552 1374 100 4464
Fragments arranged by size:
4464 1374 552 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2394 4006 4800 4820 2394
Size: 1612 794 20 4064
Fragments arranged by size:
4064 1612 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2631 2666 529
Size: 35 2067 35 4353
Fragments arranged by size:
4353 2067 35 35
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3516 3937 4826
Size: 54 1425 50 275 18 1686 421 889
Cuts at: 4826 6455 8
Size: 1629 43
Fragments arranged by size:
1686 1629 1425 889 421 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2749 3068 4420 1353
Size: 698 698 319 1352 3423
Fragments arranged by size:
3423 1352 698 698 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2897 4553 5150 5937 6205 1239
Size: 393 367 898 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 898 787 597 393 367 268
EcoRI G'AATT_C
Cuts at: 3281 3799 4177 3281
Size: 518 378 5594
Fragments arranged by size:
5594 518 378
EcoRV GAT'ATC
Cuts at: 3432 3432
Size: 6490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3683 3683
Size: 6490
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 5637 1678
Size: 3959 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3657 3689 5131 5163 5205
Size: 32 135 32 2085 32 1442 32 42
Cuts at: 5205 5237 1373
Size: 32 2626
Fragments arranged by size:
2626 2085 1442 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3676 3834 4080 669
Size: 988 281 1738 158 246 3079
Fragments arranged by size:
3079 1738 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 2985 3020 748
Size: 2237 35 4218
Fragments arranged by size:
4218 2237 35
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 2781 3815 2781
Size: 1034 5456
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 3001 5604 5788 1305
Size: 1332 364 2603 184 2007
Fragments arranged by size:
2603 2007 1332 364 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4668
Fragments arranged by size:
4668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2510 3071 3530 3650 4448 4807
Size: 648 1278 66 561 459 120 798 359
Cuts at: 4807 4966 518
Size: 159 2042
Fragments arranged by size:
2042 1278 798 648 561 459 359 159
120 66
MspA1I CmG'CkG
Cuts at: 1606 2876 3906 4583 5731 5976 1606
Size: 1270 1030 677 1148 245 2120
Fragments arranged by size:
2120 1270 1148 1030 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2439 513
Size: 552 1374 4564
Fragments arranged by size:
4564 1374 552
NdeI CA'TA_TG
Cuts at: 387 3628 3710 387
Size: 3241 82 3167
Fragments arranged by size:
3241 3167 82
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 3848 5393 1132
Size: 97 2619 1545 2229
Fragments arranged by size:
2619 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3300 1348
Size: 1952 4538
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2145 1108
Size: 1037 5453
PfoI T'CCnGG_A
Cuts at: 2611 2900 2611
Size: 289 6201
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PmlI CAC'GTG
Cuts at: 2707 2707
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3056 1613
Size: 1443 5047
PsiI TTA'TAA
Cuts at: 3071 4060 3071
Size: 989 5501
PstI C_TGCA'G
Cuts at: 830 3842 830
Size: 3012 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2876 2876
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 2463 3153 3231 3838 4973 5654 5845
Size: 219 1418 690 78 607 1135 681 191
Cuts at: 5845 826
Size: 1471
Fragments arranged by size:
1471 1418 1135 690 681 607 219 191
78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4594 5495 5757 820
Size: 197 926 2651 901 262 1553
Fragments arranged by size:
2651 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 3504 4414 5
Size: 47 3452 910 2081
Fragments arranged by size:
3452 2081 910 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6490
StyI C'CwwG_G
Cuts at: 513 1065 2100 2439 2926 3054 3929 513
Size: 552 1035 339 487 128 875 3074
Fragments arranged by size:
3074 1035 875 552 487 339 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3622 4517 4534 4687 4872 5317 325
Size: 1255 2042 895 17 153 185 445 1498
Fragments arranged by size:
2042 1498 1255 895 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 3713 4736
Size: 274 80 33 51 1443 1592 144 1023
Cuts at: 4736 6448 96
Size: 1712 138
Fragments arranged by size:
1712 1592 1443 1023 274 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3480 3512 6450 6482 3480
Size: 32 2938 32 3488
Fragments arranged by size:
3488 2938 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3270 3270
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 3281 3525 4619 2012
Size: 1269 244 1094 3883
Fragments arranged by size:
3883 1269 1094 244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI