(Circular) (Six-base) MAPSORT of: pFN21AB9610.seq Check: 2925 from: 1 to: 6274 pFN21AB9610 6274 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2224 2224 Size: 6274 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3058 3595 3058 Size: 537 5737 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4337 5721 5989 1632 Size: 2705 1384 268 1917 Fragments arranged by size: 2705 1917 1384 268 AfeI AGC'GCT Cuts at: 3117 3117 Size: 6274 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 5173 6150 1203 Size: 3970 977 1327 Fragments arranged by size: 3970 1327 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 3203 1166 Size: 2037 4237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6274 AlwNI CAG_nnn'CTG Cuts at: 2491 2671 3382 3397 3467 4017 5589 2491 Size: 180 711 15 70 550 1572 3176 Fragments arranged by size: 3176 1572 711 550 180 70 15 ApaI G_GGCC'C Cuts at: 3151 3461 3565 3151 Size: 310 104 5860 Fragments arranged by size: 5860 310 104 ApaLI G'TGCA_C Cuts at: 2130 4331 5487 2130 Size: 2201 1156 2917 Fragments arranged by size: 2917 2201 1156 ApoI r'AATT_y Cuts at: 1493 2353 3583 3809 3961 6070 1493 Size: 860 1230 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 860 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 2709 3258 3599 1870 Size: 73 766 549 341 4545 Fragments arranged by size: 4545 766 549 341 73 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2647 3058 3192 3595 Size: 325 217 627 682 178 411 134 403 Cuts at: 3595 5050 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 682 627 411 403 325 217 178 134 BanII G_rGCy'C Cuts at: 1391 2631 2694 3054 3151 3391 3461 3474 3521 Size: 1240 63 360 97 240 70 13 47 Cuts at: 3521 3528 3565 3593 1391 Size: 7 37 28 4072 Fragments arranged by size: 4072 1240 360 240 97 70 63 47 37 28 13 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4885 136 Size: 107 122 71 631 605 3213 1525 Fragments arranged by size: 3213 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 3077 3691 3077 Size: 614 5660 Bme1580I G_kGCm'C Cuts at: 1790 2038 2134 2472 2652 3151 3440 3461 3565 Size: 248 96 338 180 499 289 21 104 Cuts at: 3565 3573 4335 5491 1790 Size: 8 762 1156 2573 Fragments arranged by size: 2573 1156 762 499 338 289 248 180 104 96 21 8 BmgBI CAC'GTC Cuts at: 1318 3356 1318 Size: 2038 4236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2923 3154 4005 Size: 416 53 83 399 24 1506 231 851 Cuts at: 4005 4963 442 Size: 958 1753 Fragments arranged by size: 1753 1506 958 851 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2829 3389 3396 3512 4934 1239 Size: 974 616 560 7 116 1422 2579 Fragments arranged by size: 2579 1422 974 616 560 116 7 Bpu10I CC'TnA_GC Cuts at: 3390 3468 3390 Size: 78 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 4399 5264 5562 2750 Size: 1649 865 298 3462 Fragments arranged by size: 3462 1649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 4937 882 Size: 617 681 2757 2219 Fragments arranged by size: 2757 2219 681 617 BsaAI yAC'GTr Cuts at: 493 1204 4065 493 Size: 711 2861 2702 Fragments arranged by size: 2861 2702 711 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2526 4081 4463 5098 Size: 53 83 186 936 993 1555 382 635 Cuts at: 5098 275 Size: 1451 Fragments arranged by size: 1555 1451 993 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6049 Fragments arranged by size: 6049 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3363 1186 Size: 2177 4097 BseYI C'CCAG_C Cuts at: 3118 3290 3558 5477 5973 3118 Size: 172 268 1919 496 3419 Fragments arranged by size: 3419 1919 496 268 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 3309 3574 1163 Size: 573 864 709 265 3863 Fragments arranged by size: 3863 864 709 573 265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2134 2694 3083 3212 3391 4335 4420 5491 2134 Size: 560 389 129 179 944 85 1071 2917 Fragments arranged by size: 2917 1071 944 560 389 179 129 85 BsmI GAATG_Cn' Cuts at: 3154 3785 3878 3154 Size: 631 93 5550 Fragments arranged by size: 5550 631 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2134 2472 2631 2652 2694 3054 Size: 399 248 96 338 159 21 42 360 Cuts at: 3054 3083 3151 3212 3391 3440 3461 3474 3521 Size: 29 68 61 179 49 21 13 47 Cuts at: 3521 3528 3565 3573 3593 4335 4420 5491 1391 Size: 7 37 8 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 338 248 179 159 96 85 68 61 49 47 42 37 29 21 21 20 13 8 7 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 2499 2622 3629 844 Size: 1380 275 123 1007 3489 Fragments arranged by size: 3489 1380 1007 275 123 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4769 4943 65 Size: 1183 3521 174 1396 Fragments arranged by size: 3521 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4918 5105 1700 Size: 110 3108 187 2869 Fragments arranged by size: 3108 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 3444 794 Size: 2650 3624 BstBI TT'CG_AA Cuts at: 2358 5078 2358 Size: 2720 3554 BstEII G'GTnAC_C Cuts at: 1184 2821 2953 1184 Size: 1637 132 4505 Fragments arranged by size: 4505 1637 132 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 6274 BstYI r'GATC_y Cuts at: 1972 3604 3971 4356 4373 5825 5911 5923 6269 Size: 1632 367 385 17 1452 86 12 346 Cuts at: 6269 1972 Size: 1977 Fragments arranged by size: 1977 1632 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3480 3480 Size: 6274 BtgI C'CryG_G Cuts at: 513 1065 2004 2857 2964 513 Size: 552 939 853 107 3823 Fragments arranged by size: 3823 939 853 552 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2914 487 Size: 683 56 1688 3847 Fragments arranged by size: 3847 1688 683 56 BtsI GCAGTG_nn' Cuts at: 3187 3243 3790 4584 4604 3187 Size: 56 547 794 20 4857 Fragments arranged by size: 4857 794 547 56 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DraIII CAC_nnn'GTG Cuts at: 2740 2740 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2662 2682 3721 Size: 54 1425 50 275 18 832 20 1039 Cuts at: 3721 4610 6239 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 832 275 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2699 3217 4204 1353 Size: 723 623 518 987 3423 Fragments arranged by size: 3423 987 723 623 518 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4861 5235 5381 387 Size: 1064 138 3272 374 146 1280 Fragments arranged by size: 3272 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2692 3389 2692 Size: 697 5577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2829 3389 3396 3512 4337 4934 Size: 393 581 616 560 7 116 825 597 Cuts at: 4934 5721 5989 1239 Size: 787 268 1524 Fragments arranged by size: 1524 825 787 616 597 581 560 393 268 116 7 EcoNI CCTnn'n_nnAGG Cuts at: 2231 3479 2231 Size: 1248 5026 EcoO109I rG'GnC_Cy Cuts at: 2488 2721 3423 3562 2488 Size: 233 702 139 5200 Fragments arranged by size: 5200 702 233 139 EcoRI G'AATT_C Cuts at: 3583 3961 3583 Size: 378 5896 FspI TGC'GCA Cuts at: 2872 2981 4780 2872 Size: 109 1799 4366 Fragments arranged by size: 4366 1799 109 FspAI rTGC'GCAy Cuts at: 2981 2981 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 2671 3119 5421 1678 Size: 993 448 2302 2531 Fragments arranged by size: 2531 2302 993 448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 4915 4947 4989 Size: 32 135 32 481 32 2830 32 42 Cuts at: 4989 5021 1373 Size: 32 2626 Fragments arranged by size: 2830 2626 481 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3618 3864 669 Size: 988 281 1680 246 3079 Fragments arranged by size: 3079 1680 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6274 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3062 3599 3062 Size: 537 5737 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2096 5388 5572 1305 Size: 791 3292 184 2007 Fragments arranged by size: 3292 2007 791 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2664 2684 10 Size: 54 1425 343 832 20 3600 Fragments arranged by size: 3600 1425 832 343 54 20 MslI CAynn'nnrTG Cuts at: 518 1166 2294 3203 4232 4591 4750 518 Size: 648 1128 909 1029 359 159 2042 Fragments arranged by size: 2042 1128 1029 909 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3558 3690 4367 5515 5760 1606 Size: 1952 132 677 1148 245 2120 Fragments arranged by size: 2120 1952 1148 677 245 132 NcoI C'CATG_G Cuts at: 513 1065 2004 2857 2964 513 Size: 552 939 853 107 3823 Fragments arranged by size: 3823 939 853 552 107 NdeI CA'TA_TG Cuts at: 387 387 Size: 6274 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6274 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2557 3447 3632 5177 1132 Size: 97 1328 890 185 1545 2229 Fragments arranged by size: 2229 1545 1328 890 185 97 PasI CC'CwG_GG Cuts at: 1348 3054 3521 1348 Size: 1706 467 4101 Fragments arranged by size: 4101 1706 467 PciI A'CATG_T Cuts at: 5173 5173 Size: 6274 PflMI CCAn_nnn'nTGG Cuts at: 1108 2172 2236 2637 2660 2671 1108 Size: 1064 64 401 23 11 4711 Fragments arranged by size: 4711 1064 401 64 23 11 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 6274 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PspOMI G'GGCC_C Cuts at: 3147 3457 3561 3147 Size: 310 104 5860 Fragments arranged by size: 5860 310 104 PstI C_TGCA'G Cuts at: 830 3235 3626 830 Size: 2405 391 3478 Fragments arranged by size: 3478 2405 391 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 3558 3558 Size: 6274 SacI G_AGCT'C Cuts at: 2694 3391 2694 Size: 697 5577 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SapI GCTCTTCn'nnn_ Cuts at: 2699 3217 2699 Size: 518 5756 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 4522 6234 4522 Size: 1712 4562 SfcI C'TryA_G Cuts at: 826 1045 2757 3231 3622 4757 5438 5629 826 Size: 219 1712 474 391 1135 681 191 1471 Fragments arranged by size: 1712 1471 1135 681 474 391 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 2711 3260 3601 1872 Size: 839 549 341 4545 Fragments arranged by size: 4545 839 549 341 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 4378 5279 5541 820 Size: 197 926 822 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 822 262 197 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3632 1132 Size: 2500 3774 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6274 StyI C'CwwG_G Cuts at: 513 1065 2004 2125 2620 2857 2964 3161 3713 Size: 552 939 121 495 237 107 197 552 Cuts at: 3713 513 Size: 3074 Fragments arranged by size: 3074 939 552 552 495 237 197 121 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 3098 3540 4301 4318 4471 4656 Size: 1255 599 919 442 761 17 153 185 Cuts at: 4656 5101 325 Size: 445 1498 Fragments arranged by size: 1498 1255 919 761 599 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 4520 6232 Size: 274 80 33 51 1443 567 1976 1712 Cuts at: 6232 96 Size: 138 Fragments arranged by size: 1976 1712 1443 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3301 3318 3333 3350 6234 6266 3301 Size: 17 15 17 2884 32 3309 Fragments arranged by size: 3309 2884 32 17 17 15 Tth111I GACn'n_nGTC Cuts at: 1316 3357 1316 Size: 2041 4233 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2169 2936 3344 2169 Size: 767 408 5099 Fragments arranged by size: 5099 767 408 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6274 XmaI C'CCGG_G Cuts at: 1870 2709 3258 3599 1870 Size: 839 549 341 4545 Fragments arranged by size: 4545 839 549 341 XmnI GAAnn'nnTTC Cuts at: 4403 4403 Size: 6274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI