################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AlwI 571 GATCC GGATC(4/5) AspHI 55 GAGCAC G[AT]GC[AT]^C BsiHKAI 55 GAGCAC G[AT]GC[AT]^C BsmFI 198 GTCCC GGGAC(10/14) BsrI 405 ACTGG ACTGG(1/-1) BstYI 570 AGATCC [GA]^GATC[CT] Cac8I 183 GCAAGC GC[ACGT]^[ACGT]GC DdeI 391 CTCAG C^T[ACGT]AG DpnII 571 GATC ^GATC DraII 173 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 173 AGGCCCT [GA]G^G[ACGT]CC[CT] HaeIII 174 GGCC GG^CC MboI 571 GATC ^GATC MflI 570 AGATCC [GA]^GATC[CT] MslI 108 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 498 CATATG CA^TATG NdeII 571 GATC ^GATC PflMI 221 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 196 GAGTC GAGTC(4/5) PshAI 475 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 88 CTGCAG CTGCA^G Sau3AI 571 GATC ^GATC SfaNI 642 GATGC GCATC(5/9) SfcI 88 CTGCAG C^T[GA][CT]AG SmlI 294 CTCAAG C^T[CT][GA]AG TfiI 346 GATTC G^A[AT]TC Van91I 221 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 570 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 47 AGCT AG^CT 317 AGCT AvaII 209 GGTCC G^G[AT]CC 357 GGTCC BanI 106 GGCACC G^G[CT][GA]CC 378 GGTGCC BmyI 55 GAGCAC G[AGT]GC[ACT]^C 379 GTGCCC BpmI 228 CTGGAG CTGGAG(16/14) 512 CTGGAG Bsp1286I 55 GAGCAC G[AGT]GC[ACT]^C 379 GTGCCC DsaI 80 CCATGG C^C[GA][CT]GG 353 CCATGG HinfI 196 GAGTC G^A[ACGT]TC 346 GATTC HphI 23 TCACC GGTGA(8/7) 77 TCACC MaeIII 76 GTCAC ^GT[ACGT]AC 115 GTGAC NcoI 80 CCATGG C^CATGG 353 CCATGG NspI 16 ACATGC [GA]CATG^[CT] 127 ACATGC Tsp45I 76 GTCAC ^GT[GC]AC 115 GTGAC TspRI 594 CACTG CA[GC]TG[ACGT][ACGT]^ 632 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI BbvI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cfr13I EarI EcoRII EcoT14I Fnu4HI FokI ItaI Ksp632I MboII MnlI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI StyI TseI