Restriction map for
pFN21AB6408
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 350 GGTACC G^GTACC
AciI 538 GCGG CCGC(-3/-1)
AcsI 963 AAATTC [GA]^AATT[CT]
AfaI 351 GTAC GT^AC
Aor51HI 386 AGCGCT AGC^GCT
ApoI 963 AAATTC [GA]^AATT[CT]
Asp718I 350 GGTACC G^GTACC
BamHI 3 GGATCC G^GATCC
BanII 13 GGGCTC G[GA]GC[CT]^C
BciVI 317 GGATAC GTATCC(6/5)
BcnI 676 CCCGG CC^[GC]GG
BsaBI 750 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 420 GGTCTC GGTCTC(1/5)
BseRI 16 CTCCTC GAGGAG(10/8)
BspHI 746 TCATGA T^CATGA
BstAPI 715 GCAGTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 3 GGATCC [GA]^GATC[CT]
DraI 444 TTTAAA TTT^AAA
Eco47III 386 AGCGCT AGC^GCT
FauI 538 GCGGG CCCGC(4/6)
HaeII 386 AGCGCT [GA]GCGC^[CT]
KpnI 350 GGTACC GGTAC^C
MaeII 267 ACGT A^CGT
MamI 750 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3 GGATCC [GA]^GATC[CT]
MslI 756 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 522 CAGCTG C[AC]G^C[GT]G
NciI 676 CCCGG CC^[GC]GG
NcoI 709 CCATGG C^CATGG
NdeI 143 CATATG CA^TATG
NspI 603 GCATGC [GA]CATG^[CT]
PstI 397 CTGCAG CTGCA^G
PvuII 522 CAGCTG CAG^CTG
RcaI 746 TCATGA T^CATGA
RsaI 351 GTAC GT^AC
SexAI 858 ACCAGGT A^CC[AT]GGT
SphI 603 GCATGC GCATG^C
TaqI 221 TCGA T^CGA
TthHB8I 221 TCGA T^CGA
XhoII 3 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 932 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
944 CAGAGTCTG
BanI 350 GGTACC G^G[CT][GA]CC
541 GGCACC
BfaI 274 CTAG C^TAG
983 CTAG
BmyI 13 GGGCTC G[AGT]GC[ACT]^C
540 GGGCAC
BsmAI 237 GTCTC GTCTC(1/5)
421 GTCTC
Bsp1286I 13 GGGCTC G[AGT]GC[ACT]^C
540 GGGCAC
BspMI 469 ACCTGC ACCTGC(4/8)
417 GCAGGT
BsrI 207 ACTGG ACTGG(1/-1)
483 ACTGG
CfoI 81 GCGC GCG^C
387 GCGC
DdeI 696 CTCAG C^T[ACGT]AG
896 CTCAG
DsaI 670 CCGTGG C^C[GA][CT]GG
709 CCATGG
EarI 866 CTCTTC CTCTTC(1/4)
785 GAAGAG
EcoT14I 8 CCAAGG C^C[AT][AT]GG
709 CCATGG
HapII 108 CCGG C^CGG
677 CCGG
HhaI 81 GCGC GCG^C
387 GCGC
HinP1I 81 GCGC G^CGC
387 GCGC
HpaII 108 CCGG C^CGG
677 CCGG
HphI 308 TCACC GGTGA(8/7)
587 TCACC
Ksp632I 866 CTCTTC CTCTTC(1/4)
785 GAAGAG
MaeI 274 CTAG C^TAG
983 CTAG
MaeIII 190 GTCAC ^GT[ACGT]AC
988 GTGAC
MseI 437 TTAA T^TAA
445 TTAA
MspI 108 CCGG C^CGG
677 CCGG
PleI 883 GACTC GAGTC(4/5)
946 GAGTC
SfaNI 680 GATGC GCATC(5/9)
176 GCATC
SfcI 397 CTGCAG C^T[GA][CT]AG
772 CTACAG
StyI 8 CCAAGG C^C[AT][AT]GG
709 CCATGG
Tru9I 437 TTAA T^TAA
445 TTAA
Tsp45I 190 GTCAC ^GT[GC]AC
988 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI AvaII BbvI BpmI
BsaJI BsgI BsiYI BslI BsmFI
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I TspRI