(Circular) (Six-base) MAPSORT of: pFN21AB6408.seq Check: 7391 from: 1 to: 5710 pFN21AB6408 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 2355 3031 2355 Size: 676 5034 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2653 2858 3773 5157 5425 1632 Size: 1021 205 915 1384 268 1917 Fragments arranged by size: 1917 1384 1021 915 268 205 AfeI AGC'GCT Cuts at: 2393 2393 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2150 1184 Size: 966 4744 AlwNI CAG_nnn'CTG Cuts at: 2942 2954 3453 5025 2942 Size: 12 499 1572 3627 Fragments arranged by size: 3627 1572 499 12 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2968 3019 3245 3397 5506 1493 Size: 1475 51 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 2008 3040 3407 2008 Size: 1032 367 4311 Fragments arranged by size: 4311 1032 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2355 2546 3031 4486 618 Size: 325 217 627 568 191 485 1455 1842 Fragments arranged by size: 1842 1455 627 568 485 325 217 191 BanII G_rGCy'C Cuts at: 1391 2022 3029 1391 Size: 631 1007 4072 Fragments arranged by size: 4072 1007 631 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2316 3602 4812 2316 Size: 1286 1210 3214 Fragments arranged by size: 3214 1286 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2549 3771 4927 1790 Size: 759 1222 1156 2573 Fragments arranged by size: 2573 1222 1156 759 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2498 3441 4399 Size: 416 53 83 399 24 1081 943 958 Cuts at: 4399 442 Size: 1753 Fragments arranged by size: 1753 1081 958 943 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2324 2390 2418 2551 4370 1239 Size: 995 90 66 28 133 1819 2579 Fragments arranged by size: 2579 1819 995 133 90 66 28 Bpu10I CC'TnA_GC Cuts at: 2901 2901 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2431 4373 882 Size: 617 932 1942 2219 Fragments arranged by size: 2219 1942 932 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 2759 5704 2759 Size: 2945 2765 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 1186 Size: 826 4884 BseYI C'CCAG_C Cuts at: 2563 2604 2729 2896 4913 5409 2563 Size: 41 125 167 2017 496 2864 Fragments arranged by size: 2864 2017 496 167 125 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2461 2479 2614 1163 Size: 573 725 18 135 4259 Fragments arranged by size: 4259 725 573 135 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3771 3856 4927 3771 Size: 85 1071 4554 Fragments arranged by size: 4554 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2549 3029 3771 3856 4927 1391 Size: 399 232 527 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 480 399 232 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 2751 3599 5329 2751 Size: 848 1730 3132 Fragments arranged by size: 3132 1730 848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2413 2483 3065 844 Size: 1569 70 582 3489 Fragments arranged by size: 3489 1569 582 70 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 2726 794 Size: 1932 3778 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2008 3040 3407 3792 3809 5261 5347 5359 Size: 36 1032 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2675 2714 513 Size: 552 939 671 39 3509 Fragments arranged by size: 3509 939 671 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2727 3226 4020 4040 2727 Size: 499 794 20 4397 Fragments arranged by size: 4397 794 499 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 2451 3014 2451 Size: 563 5147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675 Size: 54 1425 50 275 18 1327 889 1629 Cuts at: 5675 8 Size: 43 Fragments arranged by size: 1629 1425 1327 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2785 2877 3640 1353 Size: 1432 92 763 3423 Fragments arranged by size: 3423 1432 763 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2324 2390 2418 2551 2653 2858 Size: 393 602 90 66 28 133 102 205 Cuts at: 2858 3773 4370 5157 5425 1239 Size: 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 602 597 393 268 205 133 102 90 66 28 EcoO109I rG'GnC_Cy Cuts at: 2264 2472 2867 2905 2264 Size: 208 395 38 5069 Fragments arranged by size: 5069 395 208 38 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2395 4857 1678 Size: 717 2462 2531 Fragments arranged by size: 2531 2462 717 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373 Size: 32 135 32 2779 32 42 32 2626 Fragments arranged by size: 2779 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 2359 3035 2359 Size: 676 5034 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 3668 4027 4186 518 Size: 648 1599 903 359 159 2042 Fragments arranged by size: 2042 1599 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2529 3126 3803 4951 5196 1606 Size: 923 597 677 1148 245 2120 Fragments arranged by size: 2120 1148 923 677 597 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2714 513 Size: 552 939 710 3509 Fragments arranged by size: 3509 939 710 552 NdeI CA'TA_TG Cuts at: 387 2149 387 Size: 1762 3948 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2612 3068 4613 1132 Size: 97 1383 456 1545 2229 Fragments arranged by size: 2229 1545 1383 456 97 PasI CC'CwG_GG Cuts at: 1348 2317 1348 Size: 969 4741 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2958 2958 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2472 2867 2905 2472 Size: 395 38 5277 Fragments arranged by size: 5277 395 38 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2406 3062 830 Size: 1576 656 3478 Fragments arranged by size: 3478 1576 656 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2529 2529 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SexAI A'CCwGG_T Cuts at: 2863 2863 Size: 5710 SfcI C'TryA_G Cuts at: 826 1045 2402 2777 3058 4193 4874 5065 826 Size: 219 1357 375 281 1135 681 191 1471 Fragments arranged by size: 1471 1357 1135 681 375 281 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2612 3068 1132 Size: 1480 456 3774 Fragments arranged by size: 3774 1480 456 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2714 3149 513 Size: 552 939 9 701 435 3074 Fragments arranged by size: 3074 939 701 552 435 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 2568 3737 3754 3907 4092 4537 Size: 1255 984 4 1169 17 153 185 445 Cuts at: 4537 325 Size: 1498 Fragments arranged by size: 1498 1255 1169 984 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2115 2147 2570 2602 5670 5702 2115 Size: 32 423 32 3068 32 2123 Fragments arranged by size: 3068 2123 423 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI