Restriction map for
pFN21AB6408
(Linear) (Six-base) MAPSORT of: KIBB6408.seq Check: 1176 from: 1 to: 1005
KIBB6408.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 350 1005
Size: 350 655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 853 1005
Size: 648 205 152
Fragments arranged by size:
648 205 152
AfeI AGC'GCT
Cuts at: 0 388 1005
Size: 388 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 1005
Size: 145 860
AlwNI CAG_nnn'CTG
Cuts at: 0 937 949 1005
Size: 937 12 56
Fragments arranged by size:
937 56 12
ApoI r'AATT_y
Cuts at: 0 963 1005
Size: 963 42
BamHI G'GATC_C
Cuts at: 0 3 1005
Size: 3 1002
BanI G'GyrC_C
Cuts at: 0 350 541 1005
Size: 350 191 464
Fragments arranged by size:
464 350 191
BanII G_rGCy'C
Cuts at: 0 17 1005
Size: 17 988
BciVI GTATCCnnnnn_n'
Cuts at: 0 311 1005
Size: 311 694
Bme1580I G_kGCm'C
Cuts at: 0 544 1005
Size: 544 461
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 1005
Size: 493 512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 438 1005
Size: 438 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 319 385 413 546 1005
Size: 229 90 66 28 133 459
Fragments arranged by size:
459 229 133 90 66 28
Bpu10I CC'TnA_GC
Cuts at: 0 896 1005
Size: 896 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 426 1005
Size: 426 579
BsaBI GATnn'nnATC
Cuts at: 0 754 1005
Size: 754 251
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 1005
Size: 7 998
BseYI C'CCAG_C
Cuts at: 0 558 599 724 891 1005
Size: 558 41 125 167 114
Fragments arranged by size:
558 167 125 114 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 474 609 1005
Size: 456 18 135 396
Fragments arranged by size:
456 396 135 18
Bsp1286I G_dGCh'C
Cuts at: 0 17 544 1005
Size: 17 527 461
Fragments arranged by size:
527 461 17
BspHI T'CATG_A
Cuts at: 0 746 1005
Size: 746 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 408 478 1005
Size: 408 70 527
Fragments arranged by size:
527 408 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 721 1005
Size: 721 284
BstYI r'GATC_y
Cuts at: 0 3 1005
Size: 3 1002
BtgI C'CryG_G
Cuts at: 0 670 709 1005
Size: 670 39 296
Fragments arranged by size:
670 296 39
BtsI GCAGTG_nn'
Cuts at: 0 722 1005
Size: 722 283
DraI TTT'AAA
Cuts at: 0 446 1005
Size: 446 559
EarI CTCTTCn'nnn_
Cuts at: 0 780 872 1005
Size: 780 92 133
Fragments arranged by size:
780 133 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 319 385 413 546 648 853 1005
Size: 229 90 66 28 133 102 205 152
Fragments arranged by size:
229 205 152 133 102 90 66 28
EcoO109I rG'GnC_Cy
Cuts at: 0 259 467 862 900 1005
Size: 259 208 395 38 105
Fragments arranged by size:
395 259 208 105 38
HaeII r_GCGC'y
Cuts at: 0 390 1005
Size: 390 615
KpnI G_GTAC'C
Cuts at: 0 354 1005
Size: 354 651
MslI CAynn'nnrTG
Cuts at: 0 760 1005
Size: 760 245
MspA1I CmG'CkG
Cuts at: 0 524 1005
Size: 524 481
NcoI C'CATG_G
Cuts at: 0 709 1005
Size: 709 296
NdeI CA'TA_TG
Cuts at: 0 144 1005
Size: 144 861
NspI r_CATG'y
Cuts at: 0 607 1005
Size: 607 398
PasI CC'CwG_GG
Cuts at: 0 312 1005
Size: 312 693
PfoI T'CCnGG_A
Cuts at: 0 953 1005
Size: 953 52
PpuMI rG'GwC_Cy
Cuts at: 0 467 862 900 1005
Size: 467 395 38 105
Fragments arranged by size:
467 395 105 38
PstI C_TGCA'G
Cuts at: 0 401 1005
Size: 401 604
PvuII CAG'CTG
Cuts at: 0 524 1005
Size: 524 481
SexAI A'CCwGG_T
Cuts at: 0 858 1005
Size: 858 147
SfcI C'TryA_G
Cuts at: 0 397 772 1005
Size: 397 375 233
Fragments arranged by size:
397 375 233
SphI G_CATG'C
Cuts at: 0 607 1005
Size: 607 398
StyI C'CwwG_G
Cuts at: 0 8 709 1005
Size: 8 701 296
Fragments arranged by size:
701 296 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 563 1005
Size: 559 4 442
Fragments arranged by size:
559 442 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 110 142 565 597 1005
Size: 110 32 423 32 408
Fragments arranged by size:
423 408 110 32 32
Enzymes that do cut:
Acc65I AcuI AfeI AlfI AlwNI ApoI BamHI BanI
BanII BciVI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaBI BseRI BseYI BsgI Bsp1286I BspHI BspMI BstAPI
BstYI BtgI BtsI DraI EarI Eco57MI EcoO109I HaeII
KpnI MslI MspA1I NcoI NdeI NspI PasI PfoI
PpuMI PstI PvuII SexAI SfcI SphI StyI TaqII
TstI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI