(Circular) (Six-base) MAPSORT of: pFN21AB6393.seq Check: 864 from: 1 to: 5533 pFN21AB6393 5533 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 2876 1937 Size: 727 212 4594 Fragments arranged by size: 4594 727 212 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3596 4980 5248 1632 Size: 1964 1384 268 1917 Fragments arranged by size: 1964 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 4432 5409 1203 Size: 3229 977 1327 Fragments arranged by size: 3229 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 2104 4262 2104 Size: 2158 3375 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 2461 3276 4848 2461 Size: 815 1572 3146 Fragments arranged by size: 3146 1572 815 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2842 3068 3220 5329 1493 Size: 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2440 2636 2858 1870 Size: 73 497 196 222 4545 Fragments arranged by size: 4545 497 222 196 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 4309 618 Size: 325 217 627 1067 1455 1842 Fragments arranged by size: 1842 1455 1067 627 325 217 BanII G_rGCy'C Cuts at: 1391 2801 2852 1391 Size: 1410 51 4072 Fragments arranged by size: 4072 1410 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2600 928 Size: 971 701 3861 Fragments arranged by size: 3861 971 701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2066 2100 3724 3758 1903 Size: 34 129 34 1624 34 3678 Fragments arranged by size: 3678 1624 129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 2331 1548 Size: 783 4750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2950 2950 Size: 5533 Bme1580I G_kGCm'C Cuts at: 1790 3594 4750 1790 Size: 1804 1156 2573 Fragments arranged by size: 2573 1804 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 4222 442 Size: 416 53 83 399 24 1847 958 1753 Fragments arranged by size: 1847 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 2551 2626 4193 1239 Size: 1244 68 75 1567 2579 Fragments arranged by size: 2579 1567 1244 75 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4196 882 Size: 617 840 1857 2219 Fragments arranged by size: 2219 1857 840 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3340 3722 4357 275 Size: 53 83 186 936 1807 382 635 1451 Fragments arranged by size: 1807 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 3958 Fragments arranged by size: 3958 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2623 1186 Size: 1117 320 4096 Fragments arranged by size: 4096 1117 320 BseYI C'CCAG_C Cuts at: 2551 4736 5232 2551 Size: 2185 496 2852 Fragments arranged by size: 2852 2185 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4960 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 2801 3594 3679 4750 2517 Size: 284 793 85 1071 3300 Fragments arranged by size: 3300 1071 793 284 85 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 BsmBI CGTCTCn'nnnn_ Cuts at: 2112 2112 Size: 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2517 2801 2852 3594 3679 4750 1391 Size: 399 727 284 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 727 399 284 85 51 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 2328 3422 5152 2328 Size: 1094 1730 2709 Fragments arranged by size: 2709 1730 1094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2377 2888 844 Size: 1533 511 3489 Fragments arranged by size: 3489 1533 511 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 4028 4202 65 Size: 1183 1452 1328 174 1396 Fragments arranged by size: 1452 1396 1328 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528 Size: 891 367 385 17 1452 86 12 346 Cuts at: 5528 1972 Size: 1977 Fragments arranged by size: 1977 1452 891 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2248 513 Size: 552 939 244 3798 Fragments arranged by size: 3798 939 552 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 2562 3049 3843 3863 2562 Size: 487 794 20 4232 Fragments arranged by size: 4232 794 487 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 4540 809 Size: 507 999 2225 1802 Fragments arranged by size: 2225 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2980 3869 Size: 54 1425 50 275 18 405 745 889 Cuts at: 3869 5498 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 745 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3463 1353 Size: 2110 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 4120 4494 4640 387 Size: 1064 138 534 1997 374 146 1280 Fragments arranged by size: 1997 1280 1064 534 374 146 138 EcoICRI GAG'CTC Cuts at: 2799 2799 Size: 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2483 2551 2626 3596 4193 4980 5248 Size: 393 851 68 75 970 597 787 268 Cuts at: 5248 1239 Size: 1524 Fragments arranged by size: 1524 970 851 787 597 393 268 75 68 EcoO109I rG'GnC_Cy Cuts at: 2046 2266 2467 2562 2632 2046 Size: 220 201 95 70 4947 Fragments arranged by size: 4947 220 201 95 70 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 4680 1678 Size: 3002 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2723 2755 4174 Size: 32 135 32 518 32 601 32 1419 Cuts at: 4174 4206 4248 4280 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1419 601 518 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 10 Size: 54 1425 343 405 3306 Fragments arranged by size: 3306 1425 405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3850 4009 518 Size: 648 2325 359 159 2042 Fragments arranged by size: 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2302 2949 3626 4774 5019 1606 Size: 696 647 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 647 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2248 513 Size: 552 939 244 3798 Fragments arranged by size: 3798 939 552 244 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2891 4436 1132 Size: 97 1662 1545 2229 Fragments arranged by size: 2229 1662 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 2424 2461 1108 Size: 1316 37 4180 Fragments arranged by size: 4180 1316 37 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2632 2467 Size: 165 5368 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PstI C_TGCA'G Cuts at: 830 2301 2628 2885 830 Size: 1471 327 257 3478 Fragments arranged by size: 3478 1471 327 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2302 2302 Size: 5533 SacI G_AGCT'C Cuts at: 2801 2801 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SexAI A'CCwGG_T Cuts at: 2469 2469 Size: 5533 SfcI C'TryA_G Cuts at: 826 1045 2297 2624 2881 4016 4697 4888 826 Size: 219 1252 327 257 1135 681 191 1471 Fragments arranged by size: 1471 1252 1135 681 327 257 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2860 1872 Size: 988 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2004 2248 2972 513 Size: 552 939 244 724 3074 Fragments arranged by size: 3074 939 724 552 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3560 3577 3730 3915 4360 325 Size: 1255 1980 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2217 2249 5493 5525 2217 Size: 32 3244 32 2225 Fragments arranged by size: 3244 2225 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2858 1870 Size: 988 4545 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI