(Circular) (Six-base) MAPSORT of: pFN21AB9760.seq Check: 3411 from: 1 to: 6922 pFN21AB9760 6922 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2263 2263 Size: 6922 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2619 4265 1937 Size: 682 1646 4594 Fragments arranged by size: 4594 1646 682 Acc65I G'GTAC_C Cuts at: 3364 4243 3364 Size: 879 6043 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3785 4985 6369 6637 1632 Size: 2153 1200 1384 268 1917 Fragments arranged by size: 2153 1917 1384 1200 268 AfeI AGC'GCT Cuts at: 2636 2649 2636 Size: 13 6909 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 5821 6798 1203 Size: 4618 977 1327 Fragments arranged by size: 4618 1327 977 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 5651 5651 Size: 6922 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3920 1184 Size: 2736 4186 AlwNI CAG_nnn'CTG Cuts at: 2177 2745 3341 3542 3733 4665 6237 2177 Size: 568 596 201 191 932 1572 2862 Fragments arranged by size: 2862 1572 932 596 568 201 191 ApaI G_GGCC'C Cuts at: 2195 2398 2530 3029 3077 3229 3276 3483 2195 Size: 203 132 499 48 152 47 207 5634 Fragments arranged by size: 5634 499 207 203 152 132 48 47 ApaLI G'TGCA_C Cuts at: 3551 4979 6135 3551 Size: 1428 1156 4338 Fragments arranged by size: 4338 1428 1156 ApoI r'AATT_y Cuts at: 1493 4231 4457 4609 6718 1493 Size: 2738 226 152 2109 1697 Fragments arranged by size: 2738 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2793 2793 Size: 6922 AseI AT'TA_AT Cuts at: 160 1032 5476 160 Size: 872 4444 1606 Fragments arranged by size: 4444 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 2194 2203 2213 2225 2267 2444 3328 Size: 73 251 9 10 12 42 177 884 Cuts at: 3328 3382 4247 1870 Size: 54 865 4545 Fragments arranged by size: 4545 884 865 251 177 73 54 42 12 10 9 AvrII C'CTAG_G Cuts at: 3844 3844 Size: 6922 BamHI G'GATC_C Cuts at: 2198 4252 4619 2198 Size: 2054 367 4501 Fragments arranged by size: 4501 2054 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3101 3289 3364 3848 3949 Size: 325 217 627 1314 188 75 484 101 Cuts at: 3949 4195 4243 5698 618 Size: 246 48 1455 1842 Fragments arranged by size: 1842 1455 1314 627 484 325 246 217 188 101 75 48 BanII G_rGCy'C Cuts at: 1391 2195 2398 2433 2530 2912 3029 3077 3229 Size: 804 203 35 97 382 117 48 152 Cuts at: 3229 3276 3407 3483 4212 4241 1391 Size: 47 131 76 729 29 4072 Fragments arranged by size: 4072 804 729 382 203 152 131 117 97 76 48 47 35 29 BbeI G_GCGC'C Cuts at: 3953 3953 Size: 6922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5951 BbvCI CC'TCA_GC Cuts at: 2187 4085 4164 2187 Size: 1898 79 4945 Fragments arranged by size: 4945 1898 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2882 2916 3634 3668 5113 5147 1903 Size: 34 945 34 718 34 1445 34 3678 Fragments arranged by size: 3678 1445 945 718 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3399 4814 6024 3399 Size: 1415 1210 4297 Fragments arranged by size: 4297 1415 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2316 2367 2402 Size: 107 122 71 631 605 644 51 35 Cuts at: 2402 2525 3233 3331 5533 136 Size: 123 708 98 2202 1525 Fragments arranged by size: 2202 1525 708 644 631 605 123 122 107 98 71 51 35 BglII A'GATC_T Cuts at: 6917 6917 Size: 6922 BlpI GC'TnA_GC Cuts at: 4339 4339 Size: 6922 Bme1580I G_kGCm'C Cuts at: 1790 2195 2398 2530 3029 3077 3106 3229 3276 Size: 405 203 132 499 48 29 123 47 Cuts at: 3276 3483 3555 4983 6139 1790 Size: 207 72 1428 1156 2573 Fragments arranged by size: 2573 1428 1156 499 405 207 203 132 123 72 48 47 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6922 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2876 3099 4653 Size: 416 53 83 399 24 1459 223 1554 Cuts at: 4653 5611 442 Size: 958 1753 Fragments arranged by size: 1753 1554 1459 958 416 399 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2885 3049 3209 3877 4172 5582 1239 Size: 1046 600 164 160 668 295 1410 2579 Fragments arranged by size: 2579 1410 1046 668 600 295 164 160 Bpu10I CC'TnA_GC Cuts at: 2187 3401 4085 4164 2187 Size: 1214 684 79 4945 Fragments arranged by size: 4945 1214 684 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2344 2636 5047 5912 6210 2344 Size: 292 2411 865 298 3056 Fragments arranged by size: 3056 2411 865 298 292 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3134 3209 5585 882 Size: 617 1635 75 2376 2219 Fragments arranged by size: 2376 2219 1635 617 75 BsaAI yAC'GTr Cuts at: 493 1204 2366 4713 493 Size: 711 1162 2347 2702 Fragments arranged by size: 2702 2347 1162 711 BsaBI GATnn'nnATC Cuts at: 2820 6916 2820 Size: 4096 2826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 3950 4729 5111 Size: 53 83 186 936 675 1742 779 382 Cuts at: 5111 5746 275 Size: 635 1451 Fragments arranged by size: 1742 1451 936 779 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2772 5355 5753 6027 6174 6585 2772 Size: 2583 398 274 147 411 3109 Fragments arranged by size: 3109 2583 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2920 2950 3185 3215 3249 Size: 30 165 30 1517 30 235 30 34 Cuts at: 3249 3279 1178 Size: 30 4821 Fragments arranged by size: 4821 1517 235 165 34 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2102 2105 2624 2950 3005 3206 3275 Size: 913 3 3 519 326 55 201 69 Cuts at: 3275 4220 1186 Size: 945 3888 Fragments arranged by size: 3888 945 913 519 326 201 69 55 3 3 BseYI C'CCAG_C Cuts at: 2045 3004 3119 3815 4057 4182 6125 6621 2045 Size: 959 115 696 242 125 1943 496 2346 Fragments arranged by size: 2346 1943 959 696 496 242 125 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3692 3843 1163 Size: 573 1956 151 4242 Fragments arranged by size: 4242 1956 573 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2130 2154 2320 2355 2536 Size: 275 186 78 51 24 166 35 181 Cuts at: 2536 2773 3987 4372 5133 6161 1540 Size: 237 1214 385 761 1028 2301 Fragments arranged by size: 2301 1214 1028 761 385 275 237 186 181 166 78 51 35 24 BsiHKAI G_wGCw'C Cuts at: 2433 3015 3555 4983 5068 6139 2433 Size: 582 540 1428 85 1071 3216 Fragments arranged by size: 3216 1428 1071 582 540 85 BsmI GAATG_Cn' Cuts at: 4433 4526 4433 Size: 93 6829 BsmBI CGTCTCn'nnnn_ Cuts at: 3727 3767 3727 Size: 40 6882 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2398 2433 2530 2912 3015 3029 Size: 399 405 203 35 97 382 103 14 Cuts at: 3029 3077 3106 3229 3276 3407 3483 3555 4212 Size: 48 29 123 47 131 76 72 657 Cuts at: 4212 4241 4983 5068 6139 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 405 399 382 203 131 123 103 97 85 76 72 48 47 35 29 29 14 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 4811 6541 4811 Size: 1730 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2263 2505 2725 3435 4277 844 Size: 1419 242 220 710 842 3489 Fragments arranged by size: 3489 1419 842 710 242 220 BsrBI CCG'CTC Cuts at: 1394 1823 2524 3370 4809 1394 Size: 429 701 846 1439 3507 Fragments arranged by size: 3507 1439 846 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5417 5591 65 Size: 1183 4169 174 1396 Fragments arranged by size: 4169 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2315 2436 2717 2757 2930 5566 Size: 110 228 277 121 281 40 173 2636 Cuts at: 5566 5753 1700 Size: 187 2869 Fragments arranged by size: 2869 2636 281 277 228 187 173 121 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6922 BssHII G'CGCG_C Cuts at: 1925 2099 2386 2388 2500 2502 2504 2538 2793 Size: 174 287 2 112 2 2 34 255 Cuts at: 2793 1925 Size: 6054 Fragments arranged by size: 6054 287 255 174 112 34 2 2 2 BssSI C'ACGA_G Cuts at: 4982 5994 4982 Size: 1012 5910 BstAPI GCAn_nnn'nTGC Cuts at: 794 2119 2177 3542 794 Size: 1325 58 1365 4174 Fragments arranged by size: 4174 1365 1325 58 BstBI TT'CG_AA Cuts at: 5726 5726 Size: 6922 BstEII G'GTnAC_C Cuts at: 1184 3457 1184 Size: 2273 4649 BstXI CCAn_nnnn'nTGG Cuts at: 2920 2920 Size: 6922 BstYI r'GATC_y Cuts at: 1972 2198 4252 4619 5004 5021 6473 6559 6571 Size: 226 2054 367 385 17 1452 86 12 Cuts at: 6571 6917 1972 Size: 346 1977 Fragments arranged by size: 2054 1977 1452 385 367 346 226 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2856 3126 3792 4107 2856 Size: 270 666 315 5671 Fragments arranged by size: 5671 666 315 270 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2530 2665 3393 3981 513 Size: 552 939 243 283 135 728 588 3454 Fragments arranged by size: 3454 939 728 588 552 283 243 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6183 Fragments arranged by size: 6183 683 56 BtsI GCAGTG_nn' Cuts at: 2342 2556 3015 3603 4438 5232 5252 2342 Size: 214 459 588 835 794 20 4012 Fragments arranged by size: 4012 835 794 588 459 214 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 4226 4226 Size: 6922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2151 2317 Size: 54 1425 50 275 18 297 24 166 Cuts at: 2317 2352 2533 2706 3600 3627 3666 3777 3984 Size: 35 181 173 894 27 39 111 207 Cuts at: 3984 4369 5258 6887 8 Size: 385 889 1629 43 Fragments arranged by size: 1629 1425 894 889 385 297 275 207 181 173 166 111 54 50 43 39 35 27 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2127 2151 2317 2352 2533 3984 4369 Size: 275 315 24 166 35 181 1451 385 Cuts at: 4369 1537 Size: 4090 Fragments arranged by size: 4090 1451 385 315 275 181 166 35 24 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 4852 1353 Size: 723 2776 3423 Fragments arranged by size: 3423 2776 723 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2878 5509 5883 6029 387 Size: 1064 138 1289 2631 374 146 1280 Fragments arranged by size: 2631 1289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2431 Size: 6922 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2885 3049 3209 3785 3877 4172 Size: 393 653 600 164 160 576 92 295 Cuts at: 4172 4985 5582 6369 6637 1239 Size: 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 653 600 597 576 393 295 268 164 160 92 EcoNI CCTnn'n_nnAGG Cuts at: 3056 3171 3257 3431 3467 3795 3056 Size: 115 86 174 36 328 6183 Fragments arranged by size: 6183 328 174 115 86 36 EcoO109I rG'GnC_Cy Cuts at: 2191 2395 2488 2860 3025 3026 3208 3225 3265 Size: 204 93 372 165 1 182 17 40 Cuts at: 3265 3272 3273 3479 3480 3539 3730 4069 2191 Size: 7 1 206 1 59 191 339 5044 Fragments arranged by size: 5044 372 339 206 204 191 182 165 93 59 40 17 7 1 1 1 EcoRI G'AATT_C Cuts at: 4231 4609 4231 Size: 378 6544 FspI TGC'GCA Cuts at: 2570 5428 2570 Size: 2858 4064 HaeII r_GCGC'y Cuts at: 1678 2297 2362 2638 2651 2701 3953 6069 1678 Size: 619 65 276 13 50 1252 2116 2531 Fragments arranged by size: 2531 2116 1252 619 276 65 50 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2825 2857 2920 2952 3185 Size: 32 135 32 1253 32 63 32 233 Cuts at: 3185 3217 3249 3281 5563 5595 5637 5669 1373 Size: 32 32 32 2282 32 42 32 2626 Fragments arranged by size: 2626 2282 1253 233 135 63 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4147 4266 4512 669 Size: 988 281 2209 119 246 3079 Fragments arranged by size: 3079 2209 988 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6922 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KasI G'GCGC_C Cuts at: 3949 3949 Size: 6922 KpnI G_GTAC'C Cuts at: 3368 4247 3368 Size: 879 6043 MfeI C'AATT_G Cuts at: 4521 4521 Size: 6922 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3183 4137 6036 6220 1305 Size: 1878 954 1899 184 2007 Fragments arranged by size: 2007 1899 1878 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 3629 10 Size: 54 1425 343 876 921 3303 Fragments arranged by size: 3303 1425 921 876 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3713 3785 4880 5239 5398 518 Size: 648 1803 744 72 1095 359 159 2042 Fragments arranged by size: 2042 1803 1095 744 648 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2174 2413 2532 2584 2667 2900 2997 3079 Size: 568 239 119 52 83 233 97 82 Cuts at: 3079 3490 3530 3592 3881 3983 4338 5015 6163 Size: 411 40 62 289 102 355 677 1148 Cuts at: 6163 6408 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 568 411 355 289 245 239 233 119 102 97 83 82 62 52 40 NaeI GCC'GGC Cuts at: 2040 2317 2040 Size: 277 6645 NarI GG'CG_CC Cuts at: 3950 3950 Size: 6922 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5431 Fragments arranged by size: 5431 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NgoMIV G'CCGG_C Cuts at: 2038 2315 2038 Size: 277 6645 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 2533 4369 2533 Size: 1836 5086 NspI r_CATG'y Cuts at: 1132 1229 3718 4280 5825 1132 Size: 97 2489 562 1545 2229 Fragments arranged by size: 2489 2229 1545 562 97 PasI CC'CwG_GG Cuts at: 1348 2905 3052 1348 Size: 1557 147 5218 Fragments arranged by size: 5218 1557 147 PciI A'CATG_T Cuts at: 5821 5821 Size: 6922 PflMI CCAn_nnn'nTGG Cuts at: 1108 3254 3475 1108 Size: 2146 221 4555 Fragments arranged by size: 4555 2146 221 PfoI T'CCnGG_A Cuts at: 3159 3241 3159 Size: 82 6840 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2860 3265 3730 2488 Size: 372 405 465 5680 Fragments arranged by size: 5680 465 405 372 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PspOMI G'GGCC_C Cuts at: 2191 2394 2526 3025 3073 3225 3272 3479 2191 Size: 203 132 499 48 152 47 207 5634 Fragments arranged by size: 5634 499 207 203 152 132 48 47 PstI C_TGCA'G Cuts at: 830 2487 3489 3535 3591 4274 830 Size: 1657 1002 46 56 683 3478 Fragments arranged by size: 3478 1657 1002 683 56 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 PvuII CAG'CTG Cuts at: 3490 3530 3592 3490 Size: 40 62 6820 Fragments arranged by size: 6820 62 40 RsrII CG'GwC_CG Cuts at: 2775 2775 Size: 6922 SacI G_AGCT'C Cuts at: 2433 2433 Size: 6922 SacII CC_GC'GG Cuts at: 2533 2668 3984 2533 Size: 135 1316 5471 Fragments arranged by size: 5471 1316 135 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SbfI CC_TGCA'GG Cuts at: 2487 4274 2487 Size: 1787 5135 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SfcI C'TryA_G Cuts at: 826 1045 2483 3485 3531 3587 4270 5405 6086 Size: 219 1438 1002 46 56 683 1135 681 Cuts at: 6086 6277 826 Size: 191 1471 Fragments arranged by size: 1471 1438 1135 1002 683 681 219 191 56 46 SfiI GGCCn_nnn'nGGCC Cuts at: 2316 2402 2525 2316 Size: 86 123 6713 Fragments arranged by size: 6713 123 86 SfoI GGC'GCC Cuts at: 3951 3951 Size: 6922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 2196 2205 2215 3330 3384 4249 1872 Size: 324 9 10 1115 54 865 4545 Fragments arranged by size: 4545 1115 865 324 54 10 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2323 2444 2651 5026 5927 6189 Size: 197 926 380 121 207 2375 901 262 Cuts at: 6189 820 Size: 1553 Fragments arranged by size: 2375 1553 926 901 380 262 207 197 121 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 3718 4280 1132 Size: 2586 562 3774 Fragments arranged by size: 3774 2586 562 SspI AAT'ATT Cuts at: 5 52 4846 5 Size: 47 4794 2081 Fragments arranged by size: 4794 2081 47 StuI AGG'CCT Cuts at: 1517 2657 3062 3801 1517 Size: 1140 405 739 4638 Fragments arranged by size: 4638 1140 739 405 StyI C'CwwG_G Cuts at: 513 1065 2004 3253 3844 4170 4212 4361 513 Size: 552 939 1249 591 326 42 149 3074 Fragments arranged by size: 3074 1249 939 591 552 326 149 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 4949 4966 5119 5304 5749 325 Size: 1255 513 2856 17 153 185 445 1498 Fragments arranged by size: 2856 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5168 6880 96 Size: 274 80 33 51 1443 3191 1712 138 Fragments arranged by size: 3191 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6882 6914 6882 Size: 32 6890 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3151 3290 3451 3151 Size: 139 161 6622 Fragments arranged by size: 6622 161 139 XhoI C'TCGA_G Cuts at: 1943 2444 1943 Size: 501 6421 XmaI C'CCGG_G Cuts at: 1870 2194 2203 2213 3328 3382 4247 1870 Size: 324 9 10 1115 54 865 4545 Fragments arranged by size: 4545 1115 865 324 54 10 9 XmnI GAAnn'nnTTC Cuts at: 5051 5051 Size: 6922 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SanDI SapI SexAI SrfI SwaI