################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AgeI ApaI AscI Asp718I AspI AviII AvrII BanI BbeI BbrPI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BssSI Bst1107I BstBI BstZ17I CelII ClaI DraI DrdI EagI EclXI Eco52I EcoRI EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AclI 223 AACGTT AA^CGTT AcsI 55 GAATTT [GA]^AATT[CT] AflIII 590 ACATGT A^C[GA][CT]GT AhdI 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 470 AGCGCT AGC^GCT ApoI 55 GAATTT [GA]^AATT[CT] AseI 1783 ATTAAT AT^TAAT AsnI 1783 ATTAAT AT^TAAT AspEI 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1359 CTCGAG C^[CT]CG[GA]G BalI 503 TGGCCA TGG^CCA BamHI 1562 GGATCC G^GATCC BbsI 786 GAAGAC GAAGAC(2/6) BclI 1200 TGATCA T^GATCA BglI 1211 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1037 AGATCT A^GATCT BpuAI 786 GAAGAC GAAGAC(2/6) BsaBI 1190 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2066 GAGACC GGTCTC(1/5) BsaWI 358 ACCGGA [AT]^CCGG[AT] BsmI 2034 GCATTC GAATGC(1/-1) BsoBI 1359 CTCGAG C^[CT]CG[GA]G Bsp1407I 305 TGTACA T^GTACA BspHI 1203 TCATGA T^CATGA BspLU11I 590 ACATGT A^CATGT BsrGI 305 TGTACA T^GTACA BssHII 836 GCGCGC G^CGCGC BstAPI 840 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 209 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 1319 CGGACCG CG^G[AT]CCG DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 470 AGCGCT AGC^GCT EcoNI 1491 CCTGACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1643 GATATC GAT^ATC FauI 1264 CCCGC CCCGC(4/6) FbaI 1200 TGATCA T^GATCA HincII 982 GTCAAC GT[CT]^[GA]AC HindII 982 GTCAAC GT[CT]^[GA]AC MamI 1190 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 503 TGGCCA TGG^CCA MscI 503 TGGCCA TGG^CCA NaeI 517 GCCGGC GCC^GGC NgoMIV 517 GCCGGC G^CCGGC PaeR7I 1359 CTCGAG C^TCGAG PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1945 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1783 ATTAAT AT^TAAT Psp1406I 223 AACGTT AA^CGTT PvuII 106 CAGCTG CAG^CTG RcaI 1203 TCATGA T^CATGA RsrII 1319 CGGACCG CG^G[AT]CCG ScaI 878 AGTACT AGT^ACT SspBI 305 TGTACA T^GTACA XhoI 1359 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Alw44I 805 GTGCAC G^TGCAC 940 GTGCAC AocI 483 CCTGAGG CC^T[ACGT]AGG 769 CCTCAGG ApaLI 805 GTGCAC G^TGCAC 940 GTGCAC Asp700I 376 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2183 GAAACTATTC BcgI 932 CGACCATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 942 GCACAACAGTCG BfaI 32 CTAG C^TAG 2017 CTAG BseRI 954 CTCCTC GAGGAG(10/8) 2048 CTCCTC BsgI 451 GTGCAG GTGCAG(16/14) 919 GTGCAG BspMI 827 ACCTGC ACCTGC(4/8) 1424 GCAGGT BsrFI 517 GCCGGC [GA]^CCGG[CT] 2105 ACCGGC BstEII 867 GGTGACC G^GT[ACGT]ACC 1636 GGTGACC BstPI 867 GGTGACC G^GT[ACGT]ACC 1636 GGTGACC Bsu36I 483 CCTGAGG CC^T[ACGT]AGG 769 CCTCAGG Cfr10I 517 GCCGGC [GA]^CCGG[CT] 2105 ACCGGC EarI 1372 GAAGAG CTCTTC(1/4) 1732 GAAGAG Eco81I 483 CCTGAGG CC^T[ACGT]AGG 769 CCTCAGG EcoO65I 867 GGTGACC G^GT[ACGT]ACC 1636 GGTGACC HaeII 470 AGCGCT [GA]GCGC^[CT] 658 AGCGCC Ksp632I 1372 GAAGAG CTCTTC(1/4) 1732 GAAGAG MaeI 32 CTAG C^TAG 2017 CTAG MaeII 224 ACGT A^CGT 620 ACGT MspA1I 106 CAGCTG C[AC]G^C[GT]G 555 CAGCGG NcoI 1566 CCATGG C^CATGG 1857 CCATGG PstI 552 CTGCAG CTGCA^G 1018 CTGCAG SmlI 198 CTCAAG C^T[CT][GA]AG 1359 CTCGAG SphI 1535 GCATGC GCATG^C 2124 GCATGC TfiI 1195 GAATC G^A[AT]TC 1630 GAATC XcmI 255 CCAGACCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1557 CCACAGGATCCATGG XmnI 376 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2183 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraIII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII