(Circular) (Six-base) MAPSORT of: pFN21AB9761.seq Check: 9855 from: 1 to: 7003 pFN21AB9761 7003 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2841 2841 Size: 7003 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4346 1937 Size: 2409 4594 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 2229 5130 5503 1563 Size: 666 2901 373 3063 Fragments arranged by size: 3063 2901 666 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3540 5066 6450 6718 1632 Size: 1908 1526 1384 268 1917 Fragments arranged by size: 1917 1908 1526 1384 268 AfeI AGC'GCT Cuts at: 2477 2477 Size: 7003 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6806 AflIII A'CryG_T Cuts at: 1203 2595 5902 6879 1203 Size: 1392 3307 977 1327 Fragments arranged by size: 3307 1392 1327 977 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 3622 5732 3622 Size: 2110 4893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7003 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7003 AlwNI CAG_nnn'CTG Cuts at: 2266 2782 3262 3620 4746 6318 2266 Size: 516 480 358 1126 1572 2951 Fragments arranged by size: 2951 1572 1126 516 480 358 ApaLI G'TGCA_C Cuts at: 2810 2945 5060 6216 2810 Size: 135 2115 1156 3597 Fragments arranged by size: 3597 2115 1156 135 ApoI r'AATT_y Cuts at: 1493 2060 4312 4538 4690 6799 1493 Size: 567 2252 226 152 2109 1697 Fragments arranged by size: 2252 2109 1697 567 226 152 AseI AT'TA_AT Cuts at: 160 1032 3789 5557 160 Size: 872 2757 1768 1606 Fragments arranged by size: 2757 1768 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 3364 4328 1870 Size: 73 1421 964 4545 Fragments arranged by size: 4545 1421 964 73 BamHI G'GATC_C Cuts at: 3567 4333 4700 3567 Size: 766 367 5870 Fragments arranged by size: 5870 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4324 5779 618 Size: 325 217 627 2537 1455 1842 Fragments arranged by size: 2537 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2184 2249 3468 3479 3932 4322 1391 Size: 793 65 1219 11 453 390 4072 Fragments arranged by size: 4072 1219 793 453 390 65 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2798 928 Size: 971 899 5133 Fragments arranged by size: 5133 971 899 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2926 2936 2960 2970 5194 5228 1903 Size: 34 989 10 24 10 2224 34 3678 Fragments arranged by size: 3678 2224 989 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 4895 6105 4895 Size: 1210 5793 BclI T'GATC_A Cuts at: 1548 3205 1548 Size: 1657 5346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3222 5614 136 Size: 107 122 71 631 605 1550 2392 1525 Fragments arranged by size: 2392 1550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3042 6998 3042 Size: 3956 3047 BlpI GC'TnA_GC Cuts at: 4420 4420 Size: 7003 Bme1580I G_kGCm'C Cuts at: 1790 2814 2949 5064 6220 1790 Size: 1024 135 2115 1156 2573 Fragments arranged by size: 2573 2115 1156 1024 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7003 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2252 4734 5692 Size: 416 53 83 399 24 835 2482 958 Cuts at: 5692 442 Size: 1753 Fragments arranged by size: 2482 1753 958 835 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2728 2947 3115 3597 4178 5663 1239 Size: 1004 485 219 168 482 581 1485 2579 Fragments arranged by size: 2579 1485 1004 581 485 482 219 168 Bpu10I CC'TnA_GC Cuts at: 3755 3755 Size: 7003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 5128 5993 6291 2188 Size: 2940 865 298 2900 Fragments arranged by size: 2940 2900 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4065 5666 882 Size: 617 2566 1601 2219 Fragments arranged by size: 2566 2219 1601 617 BsaAI yAC'GTr Cuts at: 493 1204 4794 493 Size: 711 3590 2702 Fragments arranged by size: 3590 2702 711 BsaBI GATnn'nnATC Cuts at: 3199 6997 3199 Size: 3798 3205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4810 5192 5827 275 Size: 53 83 186 936 3277 382 635 1451 Fragments arranged by size: 3277 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2363 5436 5834 6108 6255 6666 2363 Size: 3073 398 274 147 411 2700 Fragments arranged by size: 3073 2700 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2942 2972 3235 3265 3455 Size: 30 165 30 1539 30 263 30 190 Cuts at: 3455 3485 4072 4102 1178 Size: 30 587 30 4079 Fragments arranged by size: 4079 1539 587 263 190 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2950 4044 1186 Size: 1764 1094 4145 Fragments arranged by size: 4145 1764 1094 BseYI C'CCAG_C Cuts at: 2660 2913 3288 3686 3797 4255 6206 6702 2660 Size: 253 375 398 111 458 1951 496 2961 Fragments arranged by size: 2961 1951 496 458 398 375 253 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2477 2945 1163 Size: 573 741 468 5221 Fragments arranged by size: 5221 741 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4453 5214 6242 1540 Size: 275 186 2452 761 1028 2301 Fragments arranged by size: 2452 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 2814 2949 4164 5064 5149 6220 2424 Size: 390 135 1215 900 85 1071 3207 Fragments arranged by size: 3207 1215 1071 900 390 135 85 BsmI GAATG_Cn' Cuts at: 4039 4514 4607 4039 Size: 475 93 6435 Fragments arranged by size: 6435 475 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2249 2424 2814 2949 3468 3479 Size: 399 394 65 175 390 135 519 11 Cuts at: 3479 3932 4164 4322 5064 5149 6220 1391 Size: 453 232 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 453 399 394 390 232 175 158 135 85 65 11 BspEI T'CCGG_A Cuts at: 6666 6666 Size: 7003 BspHI T'CATG_A Cuts at: 3208 4892 6622 3208 Size: 1684 1730 3589 Fragments arranged by size: 3589 1730 1684 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2841 3420 4358 844 Size: 1997 579 938 3489 Fragments arranged by size: 3489 1997 938 579 BsrBI CCG'CTC Cuts at: 1394 1823 4890 1394 Size: 429 3067 3507 Fragments arranged by size: 3507 3067 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5498 5672 65 Size: 1183 4250 174 1396 Fragments arranged by size: 4250 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 4110 5647 5834 1700 Size: 110 712 1588 1537 187 2869 Fragments arranged by size: 2869 1588 1537 712 187 110 BsrGI T'GTAC_A Cuts at: 96 2310 96 Size: 2214 4789 BssHII G'CGCG_C Cuts at: 1925 2841 1925 Size: 916 6087 BssSI C'ACGA_G Cuts at: 5063 6075 5063 Size: 1012 5991 BstAPI GCAn_nnn'nTGC Cuts at: 794 2851 794 Size: 2057 4946 BstBI TT'CG_AA Cuts at: 5807 5807 Size: 7003 BstEII G'GTnAC_C Cuts at: 1184 2872 3641 1184 Size: 1688 769 4546 Fragments arranged by size: 4546 1688 769 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2221 Size: 7003 BstYI r'GATC_y Cuts at: 1972 2574 3042 3567 4333 4700 5085 5102 6554 Size: 602 468 525 766 367 385 17 1452 Cuts at: 6554 6640 6652 6998 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 766 602 525 468 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2489 2775 2489 Size: 286 6717 BtgI C'CryG_G Cuts at: 513 1065 2640 3571 3862 513 Size: 552 1575 931 291 3654 Fragments arranged by size: 3654 1575 931 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3535 487 Size: 683 56 2309 3955 Fragments arranged by size: 3955 2309 683 56 BtsI GCAGTG_nn' Cuts at: 2554 4519 5313 5333 2554 Size: 1965 794 20 4224 Fragments arranged by size: 4224 1965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6968 DraI TTT'AAA Cuts at: 4307 4307 Size: 7003 DraIII CAC_nnn'GTG Cuts at: 2455 2809 3893 2455 Size: 354 1084 5565 Fragments arranged by size: 5565 1084 354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6010 809 Size: 507 4694 1802 Fragments arranged by size: 4694 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2508 2637 Size: 54 1425 50 275 18 403 275 129 Cuts at: 2637 3627 4450 5339 6968 8 Size: 990 823 889 1629 43 Fragments arranged by size: 1629 1425 990 889 823 403 275 275 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4450 1537 Size: 275 2638 4090 Fragments arranged by size: 4090 2638 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3372 3732 4933 1353 Size: 2019 360 1201 3423 Fragments arranged by size: 3423 2019 1201 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2567 2768 3240 5590 5964 6110 Size: 1064 138 978 201 472 2350 374 146 Cuts at: 6110 387 Size: 1280 Fragments arranged by size: 2350 1280 1064 978 472 374 201 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2728 2947 3115 3540 3597 4178 Size: 393 611 485 219 168 425 57 581 Cuts at: 4178 5066 5663 6450 6718 1239 Size: 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 611 597 581 485 425 393 268 219 168 57 EcoNI CCTnn'n_nnAGG Cuts at: 3500 3500 Size: 7003 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 7003 EcoRI G'AATT_C Cuts at: 4312 4690 4312 Size: 378 6625 EcoRV GAT'ATC Cuts at: 3650 3650 Size: 7003 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2521 2749 2521 Size: 228 6775 FspI TGC'GCA Cuts at: 5509 5509 Size: 7003 HaeII r_GCGC'y Cuts at: 1678 2479 2667 6150 1678 Size: 801 188 3483 2531 Fragments arranged by size: 3483 2531 801 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2134 2140 2166 3029 Size: 32 135 32 536 26 6 26 863 Cuts at: 3029 3061 3233 3265 3317 3349 3608 3640 3967 Size: 32 172 32 52 32 259 32 327 Cuts at: 3967 3999 5644 5676 5718 5750 1373 Size: 32 1645 32 42 32 2626 Fragments arranged by size: 2626 1645 863 536 327 259 172 135 52 42 32 32 32 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2989 4347 4593 669 Size: 988 281 1051 1358 246 3079 Fragments arranged by size: 3079 1358 1051 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7003 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2189 6117 6301 1305 Size: 884 3928 184 2007 Fragments arranged by size: 3928 2007 884 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 10 Size: 54 1425 343 678 4503 Fragments arranged by size: 4503 1425 678 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 3588 3939 4167 4221 4961 5320 Size: 648 1116 1306 351 228 54 740 359 Cuts at: 5320 5479 518 Size: 159 2042 Fragments arranged by size: 2042 1306 1116 740 648 359 351 228 159 54 MspA1I CmG'CkG Cuts at: 1606 2113 2562 4419 5096 6244 6489 1606 Size: 507 449 1857 677 1148 245 2120 Fragments arranged by size: 2120 1857 1148 677 507 449 245 NaeI GCC'GGC Cuts at: 2524 2524 Size: 7003 NcoI C'CATG_G Cuts at: 513 1065 3571 3862 513 Size: 552 2506 291 3654 Fragments arranged by size: 3654 2506 552 291 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NgoMIV G'CCGG_C Cuts at: 2522 2522 Size: 7003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 2599 3544 4133 4361 5906 1132 Size: 97 1370 945 589 228 1545 2229 Fragments arranged by size: 2229 1545 1370 945 589 228 97 PasI CC'CwG_GG Cuts at: 1348 3620 1348 Size: 2272 4731 PciI A'CATG_T Cuts at: 2595 5902 2595 Size: 3307 3696 PflMI CCAn_nnn'nTGG Cuts at: 1108 2266 2626 3620 1108 Size: 1158 360 994 4491 Fragments arranged by size: 4491 1158 994 360 PfoI T'CCnGG_A Cuts at: 3149 3149 Size: 7003 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5072 5104 6585 6617 5072 Size: 32 1481 32 5458 Fragments arranged by size: 5458 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2779 Size: 7003 PshAI GACnn'nnGTC Cuts at: 1613 3954 1613 Size: 2341 4662 PsiI TTA'TAA Cuts at: 3707 4286 4573 3707 Size: 579 287 6137 Fragments arranged by size: 6137 579 287 PspXI vC'TCGA_Gb Cuts at: 3364 3364 Size: 7003 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2876 2908 2876 Size: 32 6971 PstI C_TGCA'G Cuts at: 830 2561 3027 4355 830 Size: 1731 466 1328 3478 Fragments arranged by size: 3478 1731 1328 466 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2113 2113 Size: 7003 RsrII CG'GwC_CG Cuts at: 3325 3325 Size: 7003 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 2885 5251 6963 2885 Size: 2366 1712 2925 Fragments arranged by size: 2925 2366 1712 SfcI C'TryA_G Cuts at: 826 1045 2557 2887 3023 3310 4351 5486 6167 Size: 219 1512 330 136 287 1041 1135 681 Cuts at: 6167 6358 826 Size: 191 1471 Fragments arranged by size: 1512 1471 1135 1041 681 330 287 219 191 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 4330 1872 Size: 2458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2203 3364 5107 6008 6270 820 Size: 197 926 260 1161 1743 901 262 1553 Fragments arranged by size: 1743 1553 1161 926 901 262 260 197 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7003 SphI G_CATG'C Cuts at: 1132 3544 4133 4361 1132 Size: 2412 589 228 3774 Fragments arranged by size: 3774 2412 589 228 SspI AAT'ATT Cuts at: 5 52 4927 5 Size: 47 4875 2081 Fragments arranged by size: 4875 2081 47 StuI AGG'CCT Cuts at: 1517 3280 3401 3506 1517 Size: 1763 121 105 5014 Fragments arranged by size: 5014 1763 121 105 StyI C'CwwG_G Cuts at: 513 1065 3571 3862 4197 4442 513 Size: 552 2506 291 335 245 3074 Fragments arranged by size: 3074 2506 552 335 291 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2911 3254 3660 5030 5047 5200 5385 Size: 1255 1331 343 406 1370 17 153 185 Cuts at: 5385 5830 325 Size: 445 1498 Fragments arranged by size: 1498 1370 1331 1255 445 406 343 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2583 2883 Size: 274 80 33 51 1443 333 273 300 Cuts at: 2883 5249 6961 96 Size: 2366 1712 138 Fragments arranged by size: 2366 1712 1443 333 300 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3235 3267 3455 3487 6963 6995 3235 Size: 32 188 32 3476 32 3243 Fragments arranged by size: 3476 3243 188 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7003 XbaI T'CTAG_A Cuts at: 4339 4339 Size: 7003 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2267 3569 2267 Size: 1302 5701 XhoI C'TCGA_G Cuts at: 1943 3364 1943 Size: 1421 5582 XmaI C'CCGG_G Cuts at: 1870 4328 1870 Size: 2458 4545 XmnI GAAnn'nnTTC Cuts at: 2385 4192 5132 2385 Size: 1807 940 4256 Fragments arranged by size: 4256 1807 940 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI