Restriction map for
pFN21AB9761
(Linear) (Six-base) MAPSORT of: KIBB9761.seq Check: 4518 from: 1 to: 2298
KIBB9761.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 836 2298
Size: 836 1462
AclI AA'CG_TT
Cuts at: 0 224 2298
Size: 224 2074
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1535 2298
Size: 1535 763
AfeI AGC'GCT
Cuts at: 0 472 2298
Size: 472 1826
AflIII A'CryG_T
Cuts at: 0 590 2298
Size: 590 1708
AhdI GACnn_n'nnGTC
Cuts at: 0 1617 2298
Size: 1617 681
AlwNI CAG_nnn'CTG
Cuts at: 0 261 777 1257 1615 2298
Size: 261 516 480 358 683
Fragments arranged by size:
683 516 480 358 261
ApaLI G'TGCA_C
Cuts at: 0 805 940 2298
Size: 805 135 1358
Fragments arranged by size:
1358 805 135
ApoI r'AATT_y
Cuts at: 0 55 2298
Size: 55 2243
AseI AT'TA_AT
Cuts at: 0 1784 2298
Size: 1784 514
AvaI C'yCGr_G
Cuts at: 0 1359 2298
Size: 1359 939
BamHI G'GATC_C
Cuts at: 0 1562 2298
Size: 1562 736
BanII G_rGCy'C
Cuts at: 0 179 244 1463 1474 1927 2298
Size: 179 65 1219 11 453 371
Fragments arranged by size:
1219 453 371 179 65 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 793 2298
Size: 793 1505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 921 931 955 965 2298
Size: 921 10 24 10 1333
Fragments arranged by size:
1333 921 24 10 10
BclI T'GATC_A
Cuts at: 0 1200 2298
Size: 1200 1098
BglI GCCn_nnn'nGGC
Cuts at: 0 1217 2298
Size: 1217 1081
BglII A'GATC_T
Cuts at: 0 1037 2298
Size: 1037 1261
Bme1580I G_kGCm'C
Cuts at: 0 809 944 2298
Size: 809 135 1354
Fragments arranged by size:
1354 809 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 247 2298
Size: 247 2051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 339 2298
Size: 339 1959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 723 942 1110 1592 2173 2298
Size: 238 485 219 168 482 581 125
Fragments arranged by size:
581 485 482 238 219 168 125
Bpu10I CC'TnA_GC
Cuts at: 0 1750 2298
Size: 1750 548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 2298
Size: 183 2115
BsaI GGTCTCn'nnnn_
Cuts at: 0 2060 2298
Size: 2060 238
BsaBI GATnn'nnATC
Cuts at: 0 1194 2298
Size: 1194 1104
BsaWI w'CCGG_w
Cuts at: 0 358 2298
Size: 358 1940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 937 967 1230 1260 1450 1480 2067 2097
Size: 937 30 263 30 190 30 587 30
Cuts at: 2097 2298
Size: 201
Fragments arranged by size:
937 587 263 201 190 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 945 2039 2298
Size: 945 1094 259
Fragments arranged by size:
1094 945 259
BseYI C'CCAG_C
Cuts at: 0 655 908 1283 1681 1792 2250 2298
Size: 655 253 375 398 111 458 48
Fragments arranged by size:
655 458 398 375 253 111 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 940 2298
Size: 472 468 1358
Fragments arranged by size:
1358 472 468
BsiHKAI G_wGCw'C
Cuts at: 0 419 809 944 2159 2298
Size: 419 390 135 1215 139
Fragments arranged by size:
1215 419 390 139 135
BsmI GAATG_Cn'
Cuts at: 0 2034 2298
Size: 2034 264
Bsp1286I G_dGCh'C
Cuts at: 0 179 244 419 809 944 1463 1474 1927
Size: 179 65 175 390 135 519 11 453
Cuts at: 1927 2159 2298
Size: 232 139
Fragments arranged by size:
519 453 390 232 179 175 139 135
65 11
BspHI T'CATG_A
Cuts at: 0 1203 2298
Size: 1203 1095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 836 1415 2298
Size: 836 579 883
Fragments arranged by size:
883 836 579
BsrFI r'CCGG_y
Cuts at: 0 517 2105 2298
Size: 517 1588 193
Fragments arranged by size:
1588 517 193
BsrGI T'GTAC_A
Cuts at: 0 305 2298
Size: 305 1993
BssHII G'CGCG_C
Cuts at: 0 836 2298
Size: 836 1462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 846 2298
Size: 846 1452
BstEII G'GTnAC_C
Cuts at: 0 867 1636 2298
Size: 867 769 662
Fragments arranged by size:
867 769 662
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 2298
Size: 216 2082
BstYI r'GATC_y
Cuts at: 0 569 1037 1562 2298
Size: 569 468 525 736
Fragments arranged by size:
736 569 525 468
Bsu36I CC'TnA_GG
Cuts at: 0 484 770 2298
Size: 484 286 1528
Fragments arranged by size:
1528 484 286
BtgI C'CryG_G
Cuts at: 0 635 1566 1857 2298
Size: 635 931 291 441
Fragments arranged by size:
931 635 441 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1530 2298
Size: 1530 768
BtsI GCAGTG_nn'
Cuts at: 0 549 2298
Size: 549 1749
DraIII CAC_nnn'GTG
Cuts at: 0 450 804 1888 2298
Size: 450 354 1084 410
Fragments arranged by size:
1084 450 410 354
EaeI y'GGCC_r
Cuts at: 0 228 503 632 1622 2298
Size: 228 275 129 990 676
Fragments arranged by size:
990 676 275 228 129
EarI CTCTTCn'nnn_
Cuts at: 0 1367 1727 2298
Size: 1367 360 571
Fragments arranged by size:
1367 571 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 562 763 1235 2298
Size: 562 201 472 1063
Fragments arranged by size:
1063 562 472 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 723 942 1110 1535 1592 2173 2298
Size: 238 485 219 168 425 57 581 125
Fragments arranged by size:
581 485 425 238 219 168 125 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1495 2298
Size: 1495 803
EcoO109I rG'GnC_Cy
Cuts at: 0 774 2298
Size: 774 1524
EcoRV GAT'ATC
Cuts at: 0 1645 2298
Size: 1645 653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 516 744 2298
Size: 516 228 1554
Fragments arranged by size:
1554 516 228
HaeII r_GCGC'y
Cuts at: 0 474 662 2298
Size: 474 188 1636
Fragments arranged by size:
1636 474 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 129 135 161 1024 1056 1228 1260
Size: 103 26 6 26 863 32 172 32
Cuts at: 1260 1312 1344 1603 1635 1962 1994 2298
Size: 52 32 259 32 327 32 304
Fragments arranged by size:
863 327 304 259 172 103 52 32
32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 0 984 2298
Size: 984 1314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 2298
Size: 184 2114
MscI TGG'CCA
Cuts at: 0 505 2298
Size: 505 1793
MslI CAynn'nnrTG
Cuts at: 0 277 1583 1934 2162 2216 2298
Size: 277 1306 351 228 54 82
Fragments arranged by size:
1306 351 277 228 82 54
MspA1I CmG'CkG
Cuts at: 0 108 557 2298
Size: 108 449 1741
Fragments arranged by size:
1741 449 108
NaeI GCC'GGC
Cuts at: 0 519 2298
Size: 519 1779
NcoI C'CATG_G
Cuts at: 0 1566 1857 2298
Size: 1566 291 441
Fragments arranged by size:
1566 441 291
NgoMIV G'CCGG_C
Cuts at: 0 517 2298
Size: 517 1781
NspI r_CATG'y
Cuts at: 0 594 1539 2128 2298
Size: 594 945 589 170
Fragments arranged by size:
945 594 589 170
PasI CC'CwG_GG
Cuts at: 0 1615 2298
Size: 1615 683
PciI A'CATG_T
Cuts at: 0 590 2298
Size: 590 1708
PflMI CCAn_nnn'nTGG
Cuts at: 0 261 621 1615 2298
Size: 261 360 994 683
Fragments arranged by size:
994 683 360 261
PfoI T'CCnGG_A
Cuts at: 0 1144 2298
Size: 1144 1154
PpuMI rG'GwC_Cy
Cuts at: 0 774 2298
Size: 774 1524
PshAI GACnn'nnGTC
Cuts at: 0 1949 2298
Size: 1949 349
PsiI TTA'TAA
Cuts at: 0 1702 2281 2298
Size: 1702 579 17
Fragments arranged by size:
1702 579 17
PspXI vC'TCGA_Gb
Cuts at: 0 1359 2298
Size: 1359 939
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 871 903 2298
Size: 871 32 1395
Fragments arranged by size:
1395 871 32
PstI C_TGCA'G
Cuts at: 0 556 1022 2298
Size: 556 466 1276
Fragments arranged by size:
1276 556 466
PvuII CAG'CTG
Cuts at: 0 108 2298
Size: 108 2190
RsrII CG'GwC_CG
Cuts at: 0 1320 2298
Size: 1320 978
ScaI AGT'ACT
Cuts at: 0 880 2298
Size: 880 1418
SfcI C'TryA_G
Cuts at: 0 552 882 1018 1305 2298
Size: 552 330 136 287 993
Fragments arranged by size:
993 552 330 287 136
SmlI C'TyrA_G
Cuts at: 0 198 1359 2298
Size: 198 1161 939
Fragments arranged by size:
1161 939 198
SphI G_CATG'C
Cuts at: 0 1539 2128 2298
Size: 1539 589 170
Fragments arranged by size:
1539 589 170
StuI AGG'CCT
Cuts at: 0 1275 1396 1501 2298
Size: 1275 121 105 797
Fragments arranged by size:
1275 797 121 105
StyI C'CwwG_G
Cuts at: 0 1566 1857 2192 2298
Size: 1566 291 335 106
Fragments arranged by size:
1566 335 291 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 906 1249 1655 2298
Size: 906 343 406 643
Fragments arranged by size:
906 643 406 343
TatI w'GTAC_w
Cuts at: 0 305 578 878 2298
Size: 305 273 300 1420
Fragments arranged by size:
1420 305 300 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1450 1482 2298
Size: 1230 32 188 32 816
Fragments arranged by size:
1230 816 188 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 262 1564 2298
Size: 262 1302 734
Fragments arranged by size:
1302 734 262
XhoI C'TCGA_G
Cuts at: 0 1359 2298
Size: 1359 939
XmnI GAAnn'nnTTC
Cuts at: 0 380 2187 2298
Size: 380 1807 111
Fragments arranged by size:
1807 380 111
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AhdI AlwNI ApaLI
ApoI AseI AvaI BamHI BanII BbsI BcgI BclI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PshAI PsiI PspXI PsrI PstI PvuII RsrII ScaI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AleI AlfI AloI
ApaI AscI AsiSI AvrII BaeI BanI BbeI BbvCI
BciVI BfrBI BlpI BmgBI BmtI BsaAI BsaHI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BssSI BstBI BstZ17I
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PspOMI PvuI SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI
ZraI