(Circular) (Six-base) MAPSORT of: pFN21AB6381.seq Check: 3016 from: 1 to: 5521 pFN21AB6381 5521 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 2408 3648 4021 1563 Size: 845 1240 373 3063 Fragments arranged by size: 3063 1240 845 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3584 4968 5236 1632 Size: 1952 1384 268 1917 Fragments arranged by size: 1952 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 4420 5397 1203 Size: 3217 977 1327 Fragments arranged by size: 3217 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2575 2607 2575 Size: 32 5489 AlwNI CAG_nnn'CTG Cuts at: 2762 3264 4836 2762 Size: 502 1572 3447 Fragments arranged by size: 3447 1572 502 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2132 2344 2830 3056 3208 5317 1493 Size: 639 212 486 226 152 2109 1697 Fragments arranged by size: 2109 1697 639 486 226 212 152 AseI AT'TA_AT Cuts at: 160 1032 2531 4075 160 Size: 872 1499 1544 1606 Fragments arranged by size: 1606 1544 1499 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2846 1870 Size: 73 903 4545 Fragments arranged by size: 4545 903 73 BamHI G'GATC_C Cuts at: 2140 2851 3218 2140 Size: 711 367 4443 Fragments arranged by size: 4443 711 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2842 4297 618 Size: 325 217 627 1055 1455 1842 Fragments arranged by size: 1842 1455 1055 627 325 217 BanII G_rGCy'C Cuts at: 1391 2227 2840 1391 Size: 836 613 4072 Fragments arranged by size: 4072 836 613 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4550 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4132 136 Size: 107 122 71 631 605 2460 1525 Fragments arranged by size: 2460 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2744 5516 2744 Size: 2772 2749 BlpI GC'TnA_GC Cuts at: 2938 2938 Size: 5521 Bme1580I G_kGCm'C Cuts at: 1790 3582 4738 1790 Size: 1792 1156 2573 Fragments arranged by size: 2573 1792 1156 BmgBI CAC'GTC Cuts at: 1318 2062 1318 Size: 744 4777 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2739 4181 1239 Size: 1500 1442 2579 Fragments arranged by size: 2579 1500 1442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2338 3646 4511 4809 2338 Size: 1308 865 298 3050 Fragments arranged by size: 3050 1308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2577 2607 2706 4184 882 Size: 617 1078 30 99 1478 2219 Fragments arranged by size: 2219 1478 1078 617 99 30 BsaAI yAC'GTr Cuts at: 493 1204 3312 493 Size: 711 2108 2702 Fragments arranged by size: 2702 2108 711 BsaBI GATnn'nnATC Cuts at: 2103 5515 2103 Size: 3412 2109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3328 3710 4345 275 Size: 53 83 186 936 1795 382 635 1451 Fragments arranged by size: 1795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3954 4352 4626 4773 5184 3954 Size: 398 274 147 411 4291 Fragments arranged by size: 4291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5521 BseYI C'CCAG_C Cuts at: 2396 4724 5220 2396 Size: 2328 496 2697 Fragments arranged by size: 2697 2328 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4948 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2971 3732 4760 1540 Size: 275 186 970 761 1028 2301 Fragments arranged by size: 2301 1028 970 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3582 3667 4738 3582 Size: 85 1071 4365 Fragments arranged by size: 4365 1071 85 BsmI GAATG_Cn' Cuts at: 2485 3032 3125 2485 Size: 547 93 4881 Fragments arranged by size: 4881 547 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2227 2840 3582 3667 4738 1391 Size: 399 437 613 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 437 399 85 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 2385 3410 5140 2385 Size: 1025 1730 2766 Fragments arranged by size: 2766 1730 1025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 2876 844 Size: 1715 317 3489 Fragments arranged by size: 3489 1715 317 BsrBI CCG'CTC Cuts at: 1394 1823 3408 1394 Size: 429 1585 3507 Fragments arranged by size: 3507 1585 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2824 4016 4190 65 Size: 1183 1576 1192 174 1396 Fragments arranged by size: 1576 1396 1192 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2643 4165 4352 1700 Size: 110 833 1522 187 2869 Fragments arranged by size: 2869 1522 833 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 2232 3581 4593 2232 Size: 1349 1012 3160 Fragments arranged by size: 3160 1349 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2159 794 Size: 1365 4156 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2729 2729 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2140 2371 2744 2851 3218 3603 3620 5072 Size: 168 231 373 107 367 385 17 1452 Cuts at: 5072 5158 5170 5516 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 373 367 346 231 168 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4969 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3831 3851 3037 Size: 794 20 4707 Fragments arranged by size: 4707 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DraIII CAC_nnn'GTG Cuts at: 2237 2237 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2968 3857 Size: 54 1425 50 275 18 216 922 889 Cuts at: 3857 5486 8 Size: 1629 43 Fragments arranged by size: 1629 1425 922 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 1537 Size: 275 1156 4090 Fragments arranged by size: 4090 1156 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2236 2711 3451 1353 Size: 883 475 740 3423 Fragments arranged by size: 3423 883 740 475 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4482 4628 387 Size: 1064 138 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2739 3584 4181 4968 5236 1239 Size: 393 1107 845 597 787 268 1524 Fragments arranged by size: 1524 1107 845 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2778 2778 Size: 5521 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 4668 1678 Size: 2990 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2023 2031 2055 2333 Size: 32 135 32 427 24 8 24 278 Cuts at: 2333 2365 4162 4194 4236 4268 1373 Size: 32 1797 32 42 32 2626 Fragments arranged by size: 2626 1797 427 278 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2809 2865 3111 669 Size: 988 281 871 56 246 3079 Fragments arranged by size: 3079 988 871 281 246 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3699 Fragments arranged by size: 3699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3479 3838 3997 518 Size: 648 2313 359 159 2042 Fragments arranged by size: 2313 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2364 2937 3614 4762 5007 1606 Size: 439 319 573 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 573 439 319 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4969 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2219 2879 4424 1132 Size: 97 990 660 1545 2229 Fragments arranged by size: 2229 1545 990 660 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2579 2579 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2778 2778 Size: 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PstI C_TGCA'G Cuts at: 830 2873 830 Size: 2043 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 PvuII CAG'CTG Cuts at: 2364 2364 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SapI GCTCTTCn'nnn_ Cuts at: 2711 2711 Size: 5521 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2191 2869 4004 4685 4876 826 Size: 219 1146 678 1135 681 191 1471 Fragments arranged by size: 1471 1146 1135 681 678 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2848 1872 Size: 976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2353 3625 4526 4788 820 Size: 197 926 410 1272 901 262 1553 Fragments arranged by size: 1553 1272 926 901 410 262 197 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2144 2960 513 Size: 552 1079 816 3074 Fragments arranged by size: 3074 1079 816 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2217 3548 3565 3718 3903 4348 325 Size: 1255 637 1331 17 153 185 445 1498 Fragments arranged by size: 1498 1331 1255 637 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2846 1870 Size: 976 4545 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI