(Circular) (Six-base) MAPSORT of: pFN21AB6354.seq Check: 8728 from: 1 to: 5590 pFN21AB6354 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2353 2353 Size: 5590 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2720 3653 5037 5305 1632 Size: 1088 933 1384 268 1917 Fragments arranged by size: 1917 1384 1088 933 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2819 1166 Size: 1653 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 2669 Size: 32 5558 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaI G_GGCC'C Cuts at: 2018 2018 Size: 5590 ApaLI G'TGCA_C Cuts at: 2737 3647 4803 2737 Size: 910 1156 3524 Fragments arranged by size: 3524 1156 910 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2241 2920 3287 2241 Size: 679 367 4544 Fragments arranged by size: 4544 679 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2066 2079 2909 1391 Size: 627 48 13 830 4072 Fragments arranged by size: 4072 830 627 48 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2449 2483 3781 3815 1903 Size: 34 50 34 428 34 1298 34 3678 Fragments arranged by size: 3678 1298 428 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2811 1548 Size: 1263 4327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2018 2741 3651 4807 1790 Size: 228 723 910 1156 2573 Fragments arranged by size: 2573 1156 910 723 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2475 1056 Size: 1419 4171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2666 4250 1239 Size: 1004 423 1584 2579 Fragments arranged by size: 2579 1584 1004 423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2444 3715 4580 4878 2444 Size: 1271 865 298 3156 Fragments arranged by size: 3156 1271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 2324 2642 4253 882 Size: 617 699 126 318 1611 2219 Fragments arranged by size: 2219 1611 699 617 318 126 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2762 1186 Size: 1576 4014 BseYI C'CCAG_C Cuts at: 2194 4793 5289 2194 Size: 2599 496 2495 Fragments arranged by size: 2599 2495 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 4372 Fragments arranged by size: 4372 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2434 3040 3801 4829 1540 Size: 275 186 433 606 761 1028 2301 Fragments arranged by size: 2301 1028 761 606 433 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 2741 3651 3736 4807 2294 Size: 447 910 85 1071 3077 Fragments arranged by size: 3077 1071 910 447 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2066 2079 2294 2741 2909 3651 Size: 399 228 48 13 215 447 168 742 Cuts at: 3651 3736 4807 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 228 215 168 85 48 13 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 2253 3479 5209 2253 Size: 1226 1730 2634 Fragments arranged by size: 2634 1730 1226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2199 2353 2945 844 Size: 1307 48 154 592 3489 Fragments arranged by size: 3489 1307 592 154 48 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2231 4234 4421 1700 Size: 110 421 2003 187 2869 Fragments arranged by size: 2869 2003 421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 2069 1925 Size: 144 5446 BssSI C'ACGA_G Cuts at: 2201 3650 4662 2201 Size: 1449 1012 3129 Fragments arranged by size: 3129 1449 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2121 794 Size: 1327 4263 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2194 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2241 2920 3287 3672 3689 5141 5227 5239 Size: 269 679 367 385 17 1452 86 12 Cuts at: 5239 5585 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 679 385 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2155 513 Size: 552 1090 3948 Fragments arranged by size: 3948 1090 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2050 2267 2741 2891 3106 3900 3920 2050 Size: 217 474 150 215 794 20 3720 Fragments arranged by size: 3720 794 474 217 215 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2136 3037 Size: 54 1425 50 275 18 259 47 901 Cuts at: 3037 3926 5555 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 901 889 275 259 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2084 2303 3520 1353 Size: 689 42 219 1217 3423 Fragments arranged by size: 3423 1217 689 219 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2383 4177 4551 4697 387 Size: 1064 138 794 1794 374 146 1280 Fragments arranged by size: 1794 1280 1064 794 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2666 2720 3653 4250 5037 5305 Size: 393 611 423 54 933 597 787 268 Cuts at: 5305 1239 Size: 1524 Fragments arranged by size: 1524 933 787 611 597 423 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2014 2015 2695 2014 Size: 1 680 4909 Fragments arranged by size: 4909 680 1 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2238 4737 1678 Size: 560 2499 2531 Fragments arranged by size: 2531 2499 560 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 2119 3189 2119 Size: 1070 4520 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 10 Size: 54 1425 343 259 3509 Fragments arranged by size: 3509 1425 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 3548 3907 4066 518 Size: 648 1653 729 359 159 2042 Fragments arranged by size: 2042 1653 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2248 2376 3006 3683 4831 5076 1606 Size: 543 99 128 630 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 543 245 128 99 NaeI GCC'GGC Cuts at: 2233 2233 Size: 5590 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5038 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NgoMIV G'CCGG_C Cuts at: 2231 2231 Size: 5590 NheI G'CTAG_C Cuts at: 1052 2471 1052 Size: 1419 4171 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2179 2422 2948 4493 1132 Size: 97 950 243 526 1545 2229 Fragments arranged by size: 2229 1545 950 526 243 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PfoI T'CCnGG_A Cuts at: 2643 2643 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2695 Size: 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2014 2014 Size: 5590 PstI C_TGCA'G Cuts at: 830 2013 2148 2886 2942 830 Size: 1183 135 738 56 3478 Fragments arranged by size: 3478 1183 738 135 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2149 2149 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SapI GCTCTTCn'nnn_ Cuts at: 2042 2084 2303 2042 Size: 42 219 5329 Fragments arranged by size: 5329 219 42 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2009 2144 2882 2938 4073 4754 4945 Size: 219 964 135 738 56 1135 681 191 Cuts at: 4945 826 Size: 1471 Fragments arranged by size: 1471 1135 964 738 681 219 191 135 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 3694 4595 4857 820 Size: 197 926 480 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 480 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 3029 513 Size: 552 1964 3074 Fragments arranged by size: 3074 1964 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI