Restriction map for
pFN21AB6534
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI MaeI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 961 CGCG CG^CG
AfaI 679 GTAC GT^AC
AlwI 1057 GGATC GGATC(4/5)
AlwNI 1022 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 601 TGGCCA TGG^CCA
BbrPI 833 CACGTG CAC^GTG
BclI 177 TGATCA T^GATCA
BcnI 953 CCGGG CC^[GC]GG
BglII 717 AGATCT A^GATCT
BsaAI 833 CACGTG [CT]AC^GT[GA]
BseRI 1012 GAGGAG GAGGAG(10/8)
BsgI 146 CTGCAC GTGCAG(16/14)
Bsp1407I 678 TGTACA T^GTACA
BspDI 751 ATCGAT AT^CGAT
BsrGI 678 TGTACA T^GTACA
BssSI 967 CTCGTG CACGAG(-5/-1)
BstAPI 1021 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 961 CGCG CG^CG
BstYI 717 AGATCT [GA]^GATC[CT]
ClaI 751 ATCGAT AT^CGAT
DraII 689 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 8 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 601 TGGCCA [CT]^GGCC[GA]
EcoNI 984 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 689 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 916 GATATC GAT^ATC
EcoT22I 290 ATGCAT ATGCA^T
FbaI 177 TGATCA T^GATCA
HapII 953 CCGG C^CGG
HpaII 953 CCGG C^CGG
KspI 960 CCGCGG CCGC^GG
MflI 717 AGATCT [GA]^GATC[CT]
MluNI 601 TGGCCA TGG^CCA
MscI 601 TGGCCA TGG^CCA
MspI 953 CCGG C^CGG
MvnI 961 CGCG CG^CG
NciI 953 CCGGG CC^[GC]GG
NcoI 902 CCATGG C^CATGG
NdeI 293 CATATG CA^TATG
NsiI 290 ATGCAT ATGCA^T
PleI 980 GAGTC GAGTC(4/5)
PmaCI 833 CACGTG CAC^GTG
PmlI 833 CACGTG CAC^GTG
PpuMI 689 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 831 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1028 CTGCAG CTGCA^G
PvuII 1031 CAGCTG CAG^CTG
RsaI 679 GTAC GT^AC
SacII 960 CCGCGG CCGC^GG
SapI 318 GCTCTTC GCTCTTC(1/4)
SmlI 883 CTCAAG C^T[CT][GA]AG
SspBI 678 TGTACA T^GTACA
StuI 992 AGGCCT AGG^CCT
Tsp45I 184 GTCAC ^GT[GC]AC
XcmI 902 CCATGGGAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 717 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 120 GGTCC G^G[AT]CC
690 GGTCC
BsaBI 1052 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1157 GATTCTCATC
BsaI 757 GGTCTC GGTCTC(1/5)
964 GGTCTC
BsiYI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCTACACAAGG
BslI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCTACACAAGG
BsrI 249 CCAGT ACTGG(1/-1)
386 CCAGT
BstNI 446 CCTGG CC^[AT]GG
693 CCTGG
BstXI 686 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
902 CCATGGGAATGG
Cfr13I 120 GGTCC G^G[ACGT]CC
690 GGTCC
DsaI 902 CCATGG C^C[GA][CT]GG
960 CCGCGG
EcoRI 3 GAATTC G^AATTC
271 GAATTC
EcoRII 446 CCTGG ^CC[AT]GG
693 CCTGG
EcoT14I 686 CCAAGG C^C[AT][AT]GG
902 CCATGG
HindIII 259 AAGCTT A^AGCTT
766 AAGCTT
MaeIII 184 GTCAC ^GT[ACGT]AC
1006 GTTAC
MamI 1052 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1157 GATTCTCATC
MvaI 446 CCTGG CC^[AT]GG
693 CCTGG
NlaIV 130 GGAGCC GG[ACGT]^[ACGT]CC
956 GGAGCC
Sau96I 120 GGTCC G^G[ACGT]CC
690 GGTCC
SfcI 1028 CTGCAG C^T[GA][CT]AG
1043 CTGTAG
SspI 353 AATATT AAT^ATT
499 AATATT
StyI 686 CCAAGG C^C[AT][AT]GG
902 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
BbvI BpmI BsaJI BsmAI BssKI
BstF5I DdeI DpnII EarI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MslI MspA1I MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I