(Circular) (Six-base) MAPSORT of: pFN21AB6534.seq Check: 2077 from: 1 to: 5875 pFN21AB6534 5875 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2517 3938 5322 5590 1632 Size: 695 190 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 695 268 190 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2837 2839 2895 4774 5751 1203 Size: 1634 2 56 1879 977 1327 Fragments arranged by size: 1879 1634 1327 977 56 2 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 3032 3618 5190 3032 Size: 586 1572 3717 Fragments arranged by size: 3717 1572 586 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2008 2276 2378 2570 2600 3184 3410 3562 Size: 515 268 102 192 30 584 226 152 Cuts at: 3562 5671 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 584 515 268 226 192 152 102 30 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 3194 1391 Size: 1803 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 2182 1548 Size: 634 5241 BfrBI ATG'CAT Cuts at: 2297 2297 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2722 5870 2722 Size: 3148 2727 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2406 2696 2750 3003 4535 1239 Size: 1167 290 54 253 1532 2579 Fragments arranged by size: 2579 1532 1167 290 253 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 4000 4865 5163 2873 Size: 1127 865 298 3585 Fragments arranged by size: 3585 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 2975 4538 882 Size: 617 1269 207 1563 2219 Fragments arranged by size: 2219 1563 1269 617 207 BsaAI yAC'GTr Cuts at: 493 1204 2840 3666 493 Size: 711 1636 826 2702 Fragments arranged by size: 2702 1636 826 711 BsaBI GATnn'nnATC Cuts at: 3061 3166 5869 3061 Size: 105 2703 3067 Fragments arranged by size: 3067 2703 105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2953 2983 1178 Size: 30 165 30 1550 30 4070 Fragments arranged by size: 4070 1550 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3032 1186 Size: 1846 4029 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2136 1163 Size: 573 400 4902 Fragments arranged by size: 4902 573 400 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 4021 5092 3936 Size: 85 1071 4719 Fragments arranged by size: 4719 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3194 3936 4021 5092 1391 Size: 399 1404 742 85 1071 2174 Fragments arranged by size: 2174 1404 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 2683 96 Size: 2587 3288 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2972 3935 4947 2972 Size: 963 1012 3900 Fragments arranged by size: 3900 1012 963 BstAPI GCAn_nnn'nTGC Cuts at: 794 3032 794 Size: 2238 3637 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2698 2914 2698 Size: 216 5659 BstYI r'GATC_y Cuts at: 1972 2722 3205 3572 3957 3974 5426 5512 5524 Size: 750 483 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 750 483 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2907 2965 513 Size: 552 1842 58 3423 Fragments arranged by size: 3423 1842 552 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2564 2787 3391 4185 4205 2148 Size: 416 223 604 794 20 3818 Fragments arranged by size: 3818 794 604 416 223 20 ClaI AT'CG_AT Cuts at: 1554 2757 1554 Size: 1203 4672 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2018 2018 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2606 3322 4211 Size: 54 1425 50 275 18 776 716 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 776 716 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2772 2868 3081 3155 3805 1353 Size: 977 442 96 213 74 650 3423 Fragments arranged by size: 3423 977 650 442 213 96 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2406 2517 2696 2750 3003 3938 Size: 393 695 79 111 179 54 253 935 Cuts at: 3938 4535 5322 5590 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 935 787 695 597 393 268 253 179 111 79 54 EcoNI CCTnn'n_nnAGG Cuts at: 2993 2993 Size: 5875 EcoO109I rG'GnC_Cy Cuts at: 2695 2695 Size: 5875 EcoRI G'AATT_C Cuts at: 2008 2276 3184 3562 2008 Size: 268 908 378 4321 Fragments arranged by size: 4321 908 378 268 EcoRV GAT'ATC Cuts at: 2923 2923 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2953 2985 4516 4548 4590 Size: 32 135 32 1381 32 1531 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 1531 1381 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 2264 2771 748 Size: 1516 507 3852 Fragments arranged by size: 3852 1516 507 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2077 4989 5173 1305 Size: 772 2912 184 2007 Fragments arranged by size: 2912 2007 772 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2608 10 Size: 54 1425 343 776 3277 Fragments arranged by size: 3277 1425 776 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 2735 2912 3833 4192 4351 518 Size: 648 1470 99 177 921 359 159 2042 Fragments arranged by size: 2042 1470 921 648 359 177 159 99 MspA1I CmG'CkG Cuts at: 1606 2967 3038 3047 3291 3968 5116 5361 1606 Size: 1361 71 9 244 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 244 71 9 NcoI C'CATG_G Cuts at: 513 1065 2907 513 Size: 552 1842 3481 Fragments arranged by size: 3481 1842 552 NdeI CA'TA_TG Cuts at: 387 2299 387 Size: 1912 3963 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NsiI A_TGCA'T Cuts at: 2299 2299 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2697 2697 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PmlI CAC'GTG Cuts at: 2840 2840 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2695 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 2840 1613 Size: 1227 4648 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 3037 3227 830 Size: 2207 190 3478 Fragments arranged by size: 3478 2207 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 3038 3038 Size: 5875 SacII CC_GC'GG Cuts at: 2968 2968 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2330 2330 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 3033 3048 3223 4358 5039 5230 826 Size: 219 1988 15 175 1135 681 191 1471 Fragments arranged by size: 1988 1471 1135 681 219 191 175 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 3979 4880 5142 820 Size: 197 926 945 1091 901 262 1553 Fragments arranged by size: 1553 1091 945 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 2360 2506 3799 5 Size: 47 2308 146 1293 2081 Fragments arranged by size: 2308 2081 1293 146 47 StuI AGG'CCT Cuts at: 1517 2999 1517 Size: 1482 4393 StyI C'CwwG_G Cuts at: 513 1065 2691 2907 3314 513 Size: 552 1626 216 407 3074 Fragments arranged by size: 3074 1626 552 407 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 3902 3919 4072 4257 4702 325 Size: 1255 863 1459 17 153 185 445 1498 Fragments arranged by size: 1498 1459 1255 863 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2683 4121 5833 Size: 274 80 33 51 1443 706 1438 1712 Cuts at: 5833 96 Size: 138 Fragments arranged by size: 1712 1443 1438 706 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2914 2914 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI