(Circular) (Six-base) MAPSORT of: pFN21AB6586.seq Check: 1348 from: 1 to: 5923 pFN21AB6586 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2824 2824 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 3266 1937 Size: 484 845 4594 Fragments arranged by size: 4594 845 484 Acc65I G'GTAC_C Cuts at: 2701 3244 2701 Size: 543 5380 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 3986 5370 5638 1632 Size: 701 1653 1384 268 1917 Fragments arranged by size: 1917 1653 1384 701 268 AflII C'TTAA_G Cuts at: 820 1017 2567 2876 820 Size: 197 1550 309 3867 Fragments arranged by size: 3867 1550 309 197 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2285 1184 Size: 1101 4822 AlwNI CAG_nnn'CTG Cuts at: 2278 3666 5238 2278 Size: 1388 1572 2963 Fragments arranged by size: 2963 1572 1388 ApaLI G'TGCA_C Cuts at: 3047 3980 5136 3047 Size: 933 1156 3834 Fragments arranged by size: 3834 1156 933 ApoI r'AATT_y Cuts at: 1493 2918 3232 3458 3610 5719 1493 Size: 1425 314 226 152 2109 1697 Fragments arranged by size: 2109 1697 1425 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2900 3248 1870 Size: 73 133 824 348 4545 Fragments arranged by size: 4545 824 348 133 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2654 2701 3244 4699 Size: 325 217 627 327 540 47 543 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 627 543 540 327 325 217 47 BanII G_rGCy'C Cuts at: 1391 2936 3242 1391 Size: 1545 306 4072 Fragments arranged by size: 4072 1545 306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 2974 928 Size: 971 560 515 3877 Fragments arranged by size: 3877 971 560 515 BbvCI CC'TCA_GC Cuts at: 2864 2864 Size: 5923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2567 2572 2601 2606 4114 Size: 34 50 34 546 5 29 5 1508 Cuts at: 4114 4148 1903 Size: 34 3678 Fragments arranged by size: 3678 1508 546 50 34 34 34 29 5 5 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 2595 1548 Size: 1047 4876 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 4534 136 Size: 107 122 71 631 605 451 2411 1525 Fragments arranged by size: 2411 1525 631 605 451 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2384 3340 2384 Size: 956 4967 Bme1580I G_kGCm'C Cuts at: 1790 3051 3984 5140 1790 Size: 1261 933 1156 2573 Fragments arranged by size: 2573 1261 1156 933 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2808 2955 3654 Size: 416 53 83 399 24 1391 147 699 Cuts at: 3654 4612 442 Size: 958 1753 Fragments arranged by size: 1753 1391 958 699 416 399 147 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2300 2966 4583 1239 Size: 1046 15 666 1617 2579 Fragments arranged by size: 2579 1617 1046 666 15 Bpu10I CC'TnA_GC Cuts at: 2864 2864 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2677 3714 493 Size: 711 1473 1037 2702 Fragments arranged by size: 2702 1473 1037 711 BsaBI GATnn'nnATC Cuts at: 2273 2727 5917 2273 Size: 454 3190 2279 Fragments arranged by size: 3190 2279 454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2745 4356 4754 5028 5175 5586 2745 Size: 1611 398 274 147 411 3082 Fragments arranged by size: 3082 1611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 1186 Size: 823 5100 BseYI C'CCAG_C Cuts at: 2129 2186 2252 5126 5622 2129 Size: 57 66 2874 496 2430 Fragments arranged by size: 2874 2430 496 66 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 1163 Size: 573 573 4777 Fragments arranged by size: 4777 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2592 3373 4134 5162 1540 Size: 275 186 591 781 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 591 275 186 BsiHKAI G_wGCw'C Cuts at: 3051 3984 4069 5140 3051 Size: 933 85 1071 3834 Fragments arranged by size: 3834 1071 933 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2936 3051 3242 3984 4069 5140 1391 Size: 399 1146 115 191 742 85 1071 2174 Fragments arranged by size: 2174 1146 1071 742 399 191 115 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2433 2824 3278 844 Size: 1589 391 454 3489 Fragments arranged by size: 3489 1589 454 391 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2401 2590 2794 4418 4592 65 Size: 1183 1153 189 204 1624 174 1396 Fragments arranged by size: 1624 1396 1183 1153 204 189 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2274 3253 3620 4005 4022 5474 5560 5572 Size: 302 979 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 979 385 367 346 302 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2046 2046 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2118 2793 3099 513 Size: 552 1053 675 306 3337 Fragments arranged by size: 3337 1053 675 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2620 3227 2620 Size: 607 5316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 3370 4259 Size: 54 1425 50 275 18 402 1138 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1138 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2084 3203 3853 1353 Size: 665 66 1119 650 3423 Fragments arranged by size: 3423 1119 665 650 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2300 2333 2966 3986 4583 5370 Size: 393 653 15 33 633 1020 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 1020 787 653 633 597 393 268 33 15 EcoO109I rG'GnC_Cy Cuts at: 2052 2101 2166 2284 2773 3162 2052 Size: 49 65 118 489 389 4813 Fragments arranged by size: 4813 489 389 118 65 49 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 2638 748 Size: 1890 4033 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 2705 3248 2705 Size: 543 5380 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 10 Size: 54 1425 343 402 3699 Fragments arranged by size: 3699 1425 402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2222 2267 3881 4240 4399 518 Size: 648 946 110 45 1614 359 159 2042 Fragments arranged by size: 2042 1614 946 648 359 159 110 45 MspA1I CmG'CkG Cuts at: 1606 2180 2383 2944 3092 3339 4016 5164 5409 Size: 574 203 561 148 247 677 1148 245 Cuts at: 5409 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 574 561 247 245 203 148 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 2245 1348 Size: 897 5026 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 2277 2739 2277 Size: 462 5461 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2773 2101 Size: 672 5251 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspXI vC'TCGA_Gb Cuts at: 2900 2900 Size: 5923 PstI C_TGCA'G Cuts at: 830 2430 2503 2762 3275 830 Size: 1600 73 259 513 3478 Fragments arranged by size: 3478 1600 513 259 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2383 2944 2383 Size: 561 5362 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2773 2773 Size: 5923 SapI GCTCTTCn'nnn_ Cuts at: 2018 2018 Size: 5923 SbfI CC_TGCA'GG Cuts at: 2430 2762 3275 2430 Size: 332 513 5078 Fragments arranged by size: 5078 513 332 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 2412 2412 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 2161 2426 2499 2758 3271 4406 5087 Size: 219 1116 265 73 259 513 1135 681 Cuts at: 5087 5278 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1116 681 513 265 259 219 191 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2078 3250 1872 Size: 206 1172 4545 Fragments arranged by size: 4545 1172 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 2876 2900 4027 4928 5190 Size: 197 926 624 309 24 1127 901 262 Cuts at: 5190 820 Size: 1553 Fragments arranged by size: 1553 1127 926 901 624 309 262 197 24 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3111 3847 5 Size: 47 3059 736 2081 Fragments arranged by size: 3059 2081 736 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2169 2782 3202 3362 513 Size: 552 1104 613 420 160 3074 Fragments arranged by size: 3074 1104 613 552 420 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 3950 3967 4120 4305 4750 325 Size: 1255 1092 1278 17 153 185 445 1498 Fragments arranged by size: 1498 1278 1255 1092 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2369 2242 Size: 127 5796 XhoI C'TCGA_G Cuts at: 1943 2900 1943 Size: 957 4966 XmaI C'CCGG_G Cuts at: 1870 2076 3248 1870 Size: 206 1172 4545 Fragments arranged by size: 4545 1172 206 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI