Restriction map for
pFN21AB8649
(Circular) (Six-base) MAPSORT of: pFN21AB8649.seq Check: 9744 from: 1 to: 5761
pFN21AB8649 5761 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2650 2704 2912 2650
Size: 54 208 5499
Fragments arranged by size:
5499 208 54
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2352 3104 1937
Size: 415 752 4594
Fragments arranged by size:
4594 752 415
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 2751 2822 3824 5208 5476 1632
Size: 788 331 71 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1002 788 331 268 71
AfeI AGC'GCT
Cuts at: 2864 2864
Size: 5761
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2844 1184
Size: 1660 4101
AlwNI CAG_nnn'CTG
Cuts at: 2266 2524 3504 5076 2266
Size: 258 980 1572 2951
Fragments arranged by size:
2951 1572 980 258
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2404 2531 3070 3296 3448 5557 1493
Size: 911 127 539 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 539 226 152 127
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2737 3086 1870
Size: 73 152 642 349 4545
Fragments arranged by size:
4545 642 349 152 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 2881 3082 4537 618
Size: 325 217 627 845 249 201 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 249 217 201
BanII G_rGCy'C
Cuts at: 1391 2425 3080 1391
Size: 1034 655 4072
Fragments arranged by size:
4072 1034 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2810 928
Size: 971 911 3879
Fragments arranged by size:
3879 971 911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2260 2294 3952 3986 1903
Size: 34 50 34 239 34 1658 34 3678
Fragments arranged by size:
3678 1658 239 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BfrBI ATG'CAT
Cuts at: 2959 2959
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2315 2972 3178 2315
Size: 657 206 4898
Fragments arranged by size:
4898 657 206
Bme1580I G_kGCm'C
Cuts at: 1790 2637 3822 4978 1790
Size: 847 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 847
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 4421 1239
Size: 1655 1527 2579
Fragments arranged by size:
2579 1655 1527
Bpu10I CC'TnA_GC
Cuts at: 2750 2750
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3886 4751 5049 3017
Size: 869 865 298 3729
Fragments arranged by size:
3729 869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 2387 3037 4424 882
Size: 617 678 210 650 1387 2219
Fragments arranged by size:
2219 1387 678 650 617 210
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 3568 3950 4585
Size: 53 83 186 936 1386 649 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1451 1386 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2028 2205 4194 4592 4866 5013 5424 2028
Size: 177 1989 398 274 147 411 2365
Fragments arranged by size:
2365 1989 411 398 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 2695 3029 1186
Size: 1303 206 334 3918
Fragments arranged by size:
3918 1303 334 206
BseYI C'CCAG_C
Cuts at: 2266 2855 4964 5460 2266
Size: 589 2109 496 2567
Fragments arranged by size:
2567 2109 589 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 2751 3822 3907 4978 2682
Size: 69 1071 85 1071 3465
Fragments arranged by size:
3465 1071 1071 85 69
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 3011 3011
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 2637 2682 2751 3080 3822 3907
Size: 399 635 212 45 69 329 742 85
Cuts at: 3907 4978 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 635 399 329 212 85
69 45
BspEI T'CCGG_A
Cuts at: 2028 2205 5424 2028
Size: 177 3219 2365
Fragments arranged by size:
3219 2365 177
BspHI T'CATG_A
Cuts at: 2466 3650 5380 2466
Size: 1184 1730 2847
Fragments arranged by size:
2847 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2650 2704 2912 3116 844
Size: 1806 54 208 204 3489
Fragments arranged by size:
3489 1806 208 204 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 2683 3821 4833 2683
Size: 1138 1012 3611
Fragments arranged by size:
3611 1138 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2727 3091 3458 3843 3860 5312 5398 5410
Size: 755 364 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 755 385 367 364 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2073 2886 2073
Size: 813 4948
BtgI C'CryG_G
Cuts at: 513 1065 2439 2691 2698 513
Size: 552 1374 252 7 3576
Fragments arranged by size:
3576 1374 552 252 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3053 487
Size: 683 56 1827 3195
Fragments arranged by size:
3195 1827 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 2881 2881
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2054 4768 809
Size: 507 738 2714 1802
Fragments arranged by size:
2714 1802 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726
Size: 54 1425 50 275 18 1378 889 1629
Cuts at: 5726 8
Size: 43
Fragments arranged by size:
1629 1425 1378 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2751 2822 2894 3824 4421 5208
Size: 393 788 331 71 72 930 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 930 788 787 597 393 331 268
72 71
EcoO109I rG'GnC_Cy
Cuts at: 2154 2154
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2291 2866 4908 1678
Size: 613 575 2042 2531
Fragments arranged by size:
2531 2042 613 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2993 3025 4402 4434 4476
Size: 32 135 32 1421 32 1377 32 42
Cuts at: 4476 4508 1373
Size: 32 2626
Fragments arranged by size:
2626 1421 1377 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2244 3719 4078 4237 518
Size: 648 1078 1475 359 159 2042
Fragments arranged by size:
2042 1475 1078 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2342 2551 2700 3177 3854 5002 5247
Size: 480 256 209 149 477 677 1148 245
Cuts at: 5247 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 480 477 256 245 209
149
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NsiI A_TGCA'T
Cuts at: 2961 2961
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2963 3119 4664 1132
Size: 97 1734 156 1545 2229
Fragments arranged by size:
2229 1734 1545 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 3830 3862 5343 5375 2717
Size: 32 1081 32 1481 32 3103
Fragments arranged by size:
3103 1481 1081 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2154 2154
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2673 2715 3027 3113 830
Size: 1843 42 312 86 3478
Fragments arranged by size:
3478 1843 312 86 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2086 2342 2551 2086
Size: 256 209 5296
Fragments arranged by size:
5296 256 209
SacII CC_GC'GG
Cuts at: 2701 2701
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2154 2154
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 2715 3113 2715
Size: 398 5363
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2669 2711 2947 3023 3109 4244 4925
Size: 219 1624 42 236 76 86 1135 681
Cuts at: 4925 5116 826
Size: 191 1471
Fragments arranged by size:
1624 1471 1135 681 236 219 191 86
76 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2739 3088 1872
Size: 867 349 4545
Fragments arranged by size:
4545 867 349
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 3865 4766 5028 820
Size: 197 926 1089 833 901 262 1553
Fragments arranged by size:
1553 1089 926 901 833 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 2963 3119 1132
Size: 1831 156 3774
Fragments arranged by size:
3774 1831 156
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 3008 1517
Size: 1491 4270
StyI C'CwwG_G
Cuts at: 513 1065 2166 3200 513
Size: 552 1101 1034 3074
Fragments arranged by size:
3074 1101 1034 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 3805 3958 4143 4588 325
Size: 1255 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 4007 5719
Size: 274 80 33 51 1443 159 1871 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1871 1712 1443 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 5721 5753 2672
Size: 32 3017 32 2680
Fragments arranged by size:
3017 2680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2737 3086 1870
Size: 867 349 4545
Fragments arranged by size:
4545 867 349
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI
SfoI SrfI SwaI XcmI