(Circular) (Six-base) MAPSORT of: pFN21AE0540.seq Check: 8522 from: 1 to: 5503 pFN21AE0540 5503 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2328 2431 2328 Size: 103 5400 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3313 278 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2846 1937 Size: 909 4594 Acc65I G'GTAC_C Cuts at: 2824 2824 Size: 5503 AclI AA'CG_TT Cuts at: 1563 3630 4003 1563 Size: 2067 373 3063 Fragments arranged by size: 3063 2067 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4950 5218 1632 Size: 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5306 AflIII A'CryG_T Cuts at: 1203 4402 5379 1203 Size: 3199 977 1327 Fragments arranged by size: 3199 1327 977 AgeI A'CCGG_T Cuts at: 4334 4334 Size: 5503 AhdI GACnn_n'nnGTC Cuts at: 4232 4232 Size: 5503 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2148 1184 Size: 964 4539 AlwNI CAG_nnn'CTG Cuts at: 2095 2468 3246 4818 2095 Size: 373 778 1572 2780 Fragments arranged by size: 2780 1572 778 373 ApaI G_GGCC'C Cuts at: 2132 2168 2132 Size: 36 5467 ApaLI G'TGCA_C Cuts at: 3560 4716 3560 Size: 1156 4347 ApoI r'AATT_y Cuts at: 1493 2812 3038 3190 5299 1493 Size: 1319 226 152 2109 1697 Fragments arranged by size: 2109 1697 1319 226 152 AseI AT'TA_AT Cuts at: 160 1032 4057 160 Size: 872 3025 1606 Fragments arranged by size: 3025 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 AvaI C'yCGr_G Cuts at: 1870 1943 2510 2828 1870 Size: 73 567 318 4545 Fragments arranged by size: 4545 567 318 73 BamHI G'GATC_C Cuts at: 2833 3200 2833 Size: 367 5136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2824 4279 618 Size: 325 217 627 1037 1455 1842 Fragments arranged by size: 1842 1455 1037 627 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2132 2168 2344 2822 1391 Size: 636 105 36 176 478 4072 Fragments arranged by size: 4072 636 478 176 105 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2485 928 Size: 971 586 3946 Fragments arranged by size: 3946 971 586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3694 3728 1903 Size: 34 50 34 1673 34 3678 Fragments arranged by size: 3678 1673 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3395 4605 3395 Size: 1210 4293 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 4861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2231 2240 4114 Size: 107 122 71 631 605 559 9 1874 Cuts at: 4114 136 Size: 1525 Fragments arranged by size: 1874 1525 631 605 559 122 107 71 9 BglII A'GATC_T Cuts at: 2751 5498 2751 Size: 2747 2756 BlpI GC'TnA_GC Cuts at: 2745 2920 2745 Size: 175 5328 Bme1580I G_kGCm'C Cuts at: 1790 2132 2168 3564 4720 1790 Size: 342 36 1396 1156 2573 Fragments arranged by size: 2573 1396 1156 342 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3234 4192 442 Size: 416 53 83 399 24 1817 958 1753 Fragments arranged by size: 1817 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5503 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 2463 4163 1239 Size: 1208 16 1700 2579 Fragments arranged by size: 2579 1700 1208 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 3628 4493 4791 2039 Size: 1589 865 298 2751 Fragments arranged by size: 2751 1589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4166 882 Size: 617 2667 2219 Fragments arranged by size: 2667 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3294 493 Size: 711 2090 2702 Fragments arranged by size: 2702 2090 711 BsaBI GATnn'nnATC Cuts at: 5497 5497 Size: 5503 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3310 3692 4327 275 Size: 53 83 186 936 1777 382 635 1451 Fragments arranged by size: 1777 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2062 3936 4334 4608 4755 5166 2062 Size: 1874 398 274 147 411 2399 Fragments arranged by size: 2399 1874 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5278 Fragments arranged by size: 5278 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5503 BseYI C'CCAG_C Cuts at: 4706 5202 4706 Size: 496 5007 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 2504 1163 Size: 573 723 45 4162 Fragments arranged by size: 4162 723 573 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 2953 3714 4742 1540 Size: 275 186 730 222 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 222 186 BsiHKAI G_wGCw'C Cuts at: 3564 3649 4720 3564 Size: 85 1071 4347 Fragments arranged by size: 4347 1071 85 BsmI GAATG_Cn' Cuts at: 3014 3107 3014 Size: 93 5410 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2132 2168 2344 2822 3564 3649 Size: 399 237 105 36 176 478 742 85 Cuts at: 3649 4720 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 237 176 105 85 36 BspEI T'CCGG_A Cuts at: 5166 5166 Size: 5503 BspHI T'CATG_A Cuts at: 3392 5122 3392 Size: 1730 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2431 2700 2858 844 Size: 1484 103 269 158 3489 Fragments arranged by size: 3489 1484 269 158 103 BsrBI CCG'CTC Cuts at: 1394 1823 2515 3390 1394 Size: 429 692 875 3507 Fragments arranged by size: 3507 875 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2679 3998 4172 65 Size: 1183 1431 1319 174 1396 Fragments arranged by size: 1431 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2579 2636 4147 4334 1700 Size: 110 769 57 1511 187 2869 Fragments arranged by size: 2869 1511 769 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5503 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5503 BssSI C'ACGA_G Cuts at: 3563 4575 3563 Size: 1012 4491 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 794 Size: 1652 3851 BstBI TT'CG_AA Cuts at: 2176 4307 2176 Size: 2131 3372 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5503 BstXI CCAn_nnnn'nTGG Cuts at: 2168 2168 Size: 5503 BstYI r'GATC_y Cuts at: 1972 2751 2833 3200 3585 3602 5054 5140 5152 Size: 779 82 367 385 17 1452 86 12 Cuts at: 5152 5498 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 779 385 367 346 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 2237 2237 Size: 5503 BtgI C'CryG_G Cuts at: 513 1065 2532 513 Size: 552 1467 3484 Fragments arranged by size: 3484 1467 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4764 Fragments arranged by size: 4764 683 56 BtsI GCAGTG_nn' Cuts at: 2398 2797 3019 3813 3833 2398 Size: 399 222 794 20 4068 Fragments arranged by size: 4068 794 399 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5468 DraI TTT'AAA Cuts at: 2807 2807 Size: 5503 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2571 4510 809 Size: 507 1255 1939 1802 Fragments arranged by size: 1939 1802 1255 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 2950 3839 Size: 54 1425 50 275 18 898 222 889 Cuts at: 3839 5468 8 Size: 1629 43 Fragments arranged by size: 1629 1425 898 889 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2728 2950 1537 Size: 275 916 222 4090 Fragments arranged by size: 4090 916 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2717 3433 1353 Size: 1364 716 3423 Fragments arranged by size: 3423 1364 716 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4090 4464 4610 387 Size: 1064 138 2501 374 146 1280 Fragments arranged by size: 2501 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2463 3566 4163 4950 5218 1239 Size: 393 815 16 1103 597 787 268 1524 Fragments arranged by size: 1524 1103 815 787 597 393 268 16 EcoNI CCTnn'n_nnAGG Cuts at: 2288 2288 Size: 5503 EcoO109I rG'GnC_Cy Cuts at: 2076 2092 2106 2128 2129 2164 2165 2223 2076 Size: 16 14 22 1 35 1 58 5356 Fragments arranged by size: 5356 58 35 22 16 14 1 1 EcoRI G'AATT_C Cuts at: 2812 3190 2812 Size: 378 5125 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5503 FspI TGC'GCA Cuts at: 4009 4009 Size: 5503 HaeII r_GCGC'y Cuts at: 1678 4650 1678 Size: 2972 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373 Size: 32 135 32 2572 32 42 32 2626 Fragments arranged by size: 2626 2572 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2847 3093 669 Size: 988 281 909 246 3079 Fragments arranged by size: 3079 988 909 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5503 HpaI GTT'AAC Cuts at: 3093 3093 Size: 5503 KpnI G_GTAC'C Cuts at: 2828 2828 Size: 5503 MfeI C'AATT_G Cuts at: 3102 3102 Size: 5503 MluI A'CGCG_T Cuts at: 5379 5379 Size: 5503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4617 4801 1305 Size: 3312 184 2007 Fragments arranged by size: 3312 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3681 Fragments arranged by size: 3681 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 3461 3820 3979 518 Size: 648 1335 960 359 159 2042 Fragments arranged by size: 2042 1335 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2297 2455 2919 3596 4744 4989 1606 Size: 691 158 464 677 1148 245 2120 Fragments arranged by size: 2120 1148 691 677 464 245 158 NaeI GCC'GGC Cuts at: 2638 2638 Size: 5503 NcoI C'CATG_G Cuts at: 513 1065 2532 513 Size: 552 1467 3484 Fragments arranged by size: 3484 1467 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5503 NgoMIV G'CCGG_C Cuts at: 2636 2636 Size: 5503 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5503 NotI GC'GGCC_GC Cuts at: 2950 2950 Size: 5503 NspI r_CATG'y Cuts at: 1132 1229 2861 4406 1132 Size: 97 1632 1545 2229 Fragments arranged by size: 2229 1632 1545 97 PasI CC'CwG_GG Cuts at: 1348 2072 2080 2161 2168 1348 Size: 724 8 81 7 4683 Fragments arranged by size: 4683 724 81 8 7 PciI A'CATG_T Cuts at: 4402 4402 Size: 5503 PflMI CCAn_nnn'nTGG Cuts at: 1108 2034 2323 1108 Size: 926 289 4288 Fragments arranged by size: 4288 926 289 PfoI T'CCnGG_A Cuts at: 2547 2547 Size: 5503 PmeI GTTT'AAAC Cuts at: 2807 2807 Size: 5503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 5085 5117 3572 Size: 32 1481 32 3958 Fragments arranged by size: 3958 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2076 2106 2076 Size: 30 5473 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5503 PsiI TTA'TAA Cuts at: 3073 3073 Size: 5503 PspOMI G'GGCC_C Cuts at: 2128 2164 2128 Size: 36 5467 PstI C_TGCA'G Cuts at: 830 2137 2262 2855 830 Size: 1307 125 593 3478 Fragments arranged by size: 3478 1307 593 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5503 PvuII CAG'CTG Cuts at: 2297 2455 2297 Size: 158 5345 SalI G'TCGA_C Cuts at: 1936 2845 1936 Size: 909 4594 SanDI GG'GwC_CC Cuts at: 2076 2076 Size: 5503 SbfI CC_TGCA'GG Cuts at: 2855 2855 Size: 5503 ScaI AGT'ACT Cuts at: 3751 5463 3751 Size: 1712 3791 SfcI C'TryA_G Cuts at: 826 1045 2133 2258 2851 3986 4667 4858 826 Size: 219 1088 125 593 1135 681 191 1471 Fragments arranged by size: 1471 1135 1088 681 593 219 191 125 SfiI GGCCn_nnn'nGGCC Cuts at: 2231 2231 Size: 5503 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5503 SmaI CCC'GGG Cuts at: 1872 2830 1872 Size: 958 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 3607 4508 4770 820 Size: 197 926 111 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 262 197 111 SnaBI TAC'GTA Cuts at: 493 3294 493 Size: 2801 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5503 SphI G_CATG'C Cuts at: 1132 2861 1132 Size: 1729 3774 SspI AAT'ATT Cuts at: 5 52 3427 5 Size: 47 3375 2081 Fragments arranged by size: 3375 2081 47 StuI AGG'CCT Cuts at: 1517 2199 1517 Size: 682 4821 StyI C'CwwG_G Cuts at: 513 1065 2532 2643 2942 513 Size: 552 1467 111 299 3074 Fragments arranged by size: 3074 1467 552 299 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2362 2574 3530 3547 3700 3885 4330 Size: 1255 782 212 956 17 153 185 445 Cuts at: 4330 325 Size: 1498 Fragments arranged by size: 1498 1255 956 782 445 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 2625 3749 Size: 274 80 33 51 1443 405 243 1124 Cuts at: 3749 5461 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1124 405 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5463 5495 5463 Size: 32 5471 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5503 XbaI T'CTAG_A Cuts at: 2839 2839 Size: 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5503 XmaI C'CCGG_G Cuts at: 1870 2828 1870 Size: 958 4545 XmnI GAAnn'nnTTC Cuts at: 3632 3632 Size: 5503 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3311 276 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI